Polyhydroxyalkanoate-containing structure and manufacturing method thereof
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
C12P-007/62
C12P-007/16
C12P-011/00
출원번호
US-0191540
(2002-07-10)
우선권정보
JP-0172978 (2002-06-13); JP-0210052 (2001-07-10)
발명자
/ 주소
Nomoto, Tsuyoshi
Yano, Tetsuya
Kozaki, Shinya
Honma, Tsutomu
출원인 / 주소
Canon Kabushiki Kaisha
대리인 / 주소
Fitzpatrick, Cella, Harper and Scinto
인용정보
피인용 횟수 :
13인용 특허 :
1
초록▼
A method for manufacturing polyhydroxyalkanoate-containing structure, at least a part of a base material surface of the structure being coated with polyhydroxyalkanoate, the method comprises the steps of immobilizing a polyhydroxyalkanoate synthase on the base material surface, synthesizing, on the
A method for manufacturing polyhydroxyalkanoate-containing structure, at least a part of a base material surface of the structure being coated with polyhydroxyalkanoate, the method comprises the steps of immobilizing a polyhydroxyalkanoate synthase on the base material surface, synthesizing, on the base material surface, polyhydroxyalkanoate using a 3-hydroxyacyl coenzyme A to become the substrate of the synthase and the synthase and coating at least a part of the base material surface with the synthesized polyhydroxyalkanoate, wherein the synthase contains an amino acid sequence capable of binding to the base material. A polyhydroxyalkanoate-containing structure, at least a part of a base material surface of the structure being coated with a polyhydroxyalkanoate, comprises the base material, a polyhydroxyalkanoate synthase immobilized on the base material surface, and the polyhydroxyalkanoate with which at least a part of the base material surface is coated, wherein the synthase contains an amino acid sequence capable of binding to the base material.
대표청구항▼
1. A method for manufacturing polyhydroxyalkanoate-containing structure, at least a part of a base material surface of the structure being coated with polyhydroxyalkanoate, the method comprising the steps of:immobilizing a polyhydroxyalkanoate synthase on said base material surface, synthesizing, on
1. A method for manufacturing polyhydroxyalkanoate-containing structure, at least a part of a base material surface of the structure being coated with polyhydroxyalkanoate, the method comprising the steps of:immobilizing a polyhydroxyalkanoate synthase on said base material surface, synthesizing, on said base material surface, polyhydroxyalkanoate using a 3-hydroxyacyl coenzyme A to become the substrate of said synthase and said synthase and coating at least a part of said base material surface with the synthesized polyhydroxyalkanoate, wherein said synthase contains an amino acid sequence capable of binding to said base material. 2. The manufacturing method according to claim 1, wherein the polyhydroxyalkanoate is comprised of at least one selected from the group consisting of monomer units expressed by Formulas [1] to [10], and each corresponding 3-hydroxyacyl coenzyme A is selected from the group consisting of 3-hydroxyacyl coenzymes A expressed by Formulas [12] to [21]: (wherein symbol “a” represents an integer, and the combination of R1 and “a” is selected from the group consisting of a combination of a hydrogen atom and any one integer selected from the group consisting of 0 to 10;a combination of a halogen atom and any one integer selected from the group consisting of 1 to 10; a combination of a chromophoric group and any one integer selected from the group consisting of 1 to 10; a combination of a carboxyl group or a salt thereof and any one integer selected from the group consisting of 1 to 10; and a combination of and any one integer selected from the group consisting of 1 to 7), (wherein b represents any one integer selected from the group consisting of 0 to 7, and R2 is selected from the group consisting of a hydrogen atom (H), a halogen atom, ?CN, ?NO2, ?CF3, ?C2F5 and ?C3F7), (wherein c represents any one integer selected from the group consisting of 1 to 8, and R3 is selected from the group consisting of a hydrogen atom (H), a halogen atom, ?CN, ?NO2, ?CF3, ?C2F5 and ?C3F7), (wherein d represents any one integer selected from the group consisting of 0 to 7, and R4 is selected from the group consisting of a hydrogen atom (H), a halogen atom, ?CN, ?NO2, ?CF3, ?C2F5 and ?C3F7), (wherein e represents any one integer selected from the group consisting of 1 to 8, and R5 is selected from the group consisting of a hydrogen atom (H), a halogen atom, ?CN, ?NO2, ?CF3, ?C2F5, C3F7, ?CH3, ?C2H5 and ?C3H7), (wherein f represents any one integer selected from the group consisting of 0 to 7), (wherein g represents any one integer selected from the group consisting of 1 to 8), (wherein h represents any one integer selected from the group consisting of 1 to 7, and R6 is selected from the group consisting of a hydrogen atom (H), a halogen atom, ?CN, ?NO2, ?COOR′, ?SO2R″, ?CH3, ?C2H5, ?C3H7, ?CH(CH3)2 and ?C(CH3)3, wherein R′ is selected from the group consisting of a hydrogen atom (H), Na, K, ?CH3 and ?C2H5, and R″ is selected from the group consisting of ?OH, ONa, ?OK, a halogen atom, ?OCH3 and ?OC2H5), (wherein i represents any one integer selected from the group consisting of 1 to 7, and R7 is selected from the group consisting of a hydrogen atom (H), a halogen atom, ?CN, ?NO2, ?COOR′ and ?SO2R″, wherein R′ is selected from the group consisting of a hydrogen atom (H), Na, K, ?CH3 and ?C2H5, and R″ is selected from the group consisting of ?OH, ?ONa, ?OK, a halogen atom, ?OCH3 and ?OC2H5), and (wherein j represents any one integer selected from the group consisting of 1 to 9), (wherein ?SCoA represents a CoA bound to alkanoic acid, symbol “a” represents an integer, and the combination of R1 and a is defined as the same as the combination of R1 and a in the monomer unit expressed by the above described Formula [1]), (wherein ?SCoA represents a CoA bound to alkanoic acid, and b and R2 are respectively defined as the same as b and R2 in the monomer unit expressed by the above described Formula [2]), (wherein ?SCoA represents a CoA bound to alkanoic acid, and c and R3 are respectively defined as the same as c and R3 in the monomer unit expressed by the above described Formula [3]), (wherein ?SCoA represents a CoA bound to alkanoic acid, and d and R4 are respectively defined as the same as d and R4 in the monomer unit expressed by the above described Formula [4]), (wherein ?SCoA represents a CoA bound to alkanoic acid, and e and R5 are respectively defined as the same as e and R5 in the monomer unit expressed by the above described Formula [5]), (wherein ?SCoA represents a CoA bound to alkanoic acid, and f is defined as the same as f in the monomer unit expressed by the above described Formula [6]), (wherein ?SCoA represents a CoA bound to alkanoic acid, and g is defined as the same as g in the monomer unit expressed by the above described Formula [7]), (wherein ?SCoA represents a CoA bound to alkanoic acid, and h and R6 are respectively defined as the same as h and R6 in the monomer unit expressed by the above described Formula [8]), (wherein ?SCoA represents a CoA bound to alkanoic acid, and i and R7 are respectively defined as the same as i and R7 in the monomer unit expressed by the above described Formula [9]), and (wherein ?SCoA represents a CoA bound to alkanoic acid, and j is defined as the same as j in the monomer unit expressed by the above described Formula [10]).3. The manufacturing method according to claim 2, whereinsaid polyhydroxyalkanoate has a carboxyl group and is comprised of at least one selected from the group consisting of monomer units expressed by Formula [11], and each corresponding 3-hydroxyacyl coenzyme A is selected from the group consisting of 3-hydroxyacyl coenzymes A expressed by Formula [22], said Formulas being: wherein k represents an integer of 1 to 10, and wherein ?SCoA in the formula represents coenzyme A binding to alkanoic acid, and k in the formula is defined as in Formula [11].4. The manufacturing method according to claim 1, whereinthe 3-hydroxyalkanoic acid unit composition of said polyhydroxyalkanoate is made to change in a lamination direction of said polyhydroxyalkanoate-containing structure by varying the composition of said 3-hydroxyacyl coenzyme A with time. 5. The manufacturing method according to claim 1, the method further comprising a step of conducting chemical modification on at least a part of polyhydroxyalkanoate, said base material being coated with the polyhydroxyalkanoate.6. The manufacturing method according to claim 5, whereinsaid step of conducting chemical modification is a step of adding a graft chain to at least a part of said polyhydroxyalkanoate. 7. The manufacturing method according to claim 6, whereinsaid step of adding a graft chain is a step of reacting at least a part of said polyhydroxyalkanoate with a compound having a reactive functional group at an end thereof. 8. The manufacturing method according to claim 6, whereinsaid polyhydroxyalkanoate is comprised of at least a monomer unit having an epoxy group. 9. The manufacturing method according to claim 7, whereinsaid compound having a reactive functional group at an end thereof is a compound having an amino group. 10. The manufacturing method according to claim 9, whereinsaid compound having an amino group is a terminal amino-modified compound. 11. The manufacturing method according to claim 10, whereinsaid terminal amino-modified compound is at least one selected from the group consisting of polyvinylamine, polyethyleneimine and terminal amino-modified polysiloxane. 12. The manufacturing method according to claim 5, whereinsaid step of conducting chemical modification is a step of crosslinking at least a part of polyhydroxyalkanoate. 13. The manufacturing method according to claim 12, whereinsaid step of crosslinking is a step of reacting at least a part of said polyhydroxyalkanoate with a crosslinking agent. 14. The manufacturing method according to claim 12, whereinsaid polyhydroxyalkanoate is comprised of at least a monomer unit having an epoxy group. 15. The manufacturing method according to claim 13, whereinsaid crosslinking agent is at least one selected from the group consisting of a diamine compound, succinic anhydride and 2-methyl-4-methylimidazole. 16. The manufacturing method according to claim 15, whereinsaid diamine compound is hexamethylenediamine. 17. The manufacturing method according to claim 12, whereinsaid step of crosslinking is a step of irradiating said polyhydroxyalkanoate with electron rays. 18. The manufacturing method according to claim 1, whereinsaid amino acid sequence capable of binding to the base material is an amino acid sequence determined by screening of a random peptide library. 19. The manufacturing method according to claim 1, whereinthe base material is copper phthalocyanine and the amino acid sequence capable of binding to said base material is all or a part of at least one amino acid sequence selected from the group consisting of followings: Lys-Tyr-Asp-Ser-Arg-His-Leu-His-Thr-His-Ser-His (SEQ ID NO:24)Pro-Asn-Arg-Leu-Gly-Arg-Arg-Pro-Val-Arg-Trp-Glu (SEQ ID NO:25)Lys-Cys-Cys-Tyr-Tyr-Asp-His-Ser-His-Ala-Leu-Ser (SEQ ID NO:26)Glu-Tyr-Leu-Ser-Ala-Ile-Val-Ala-Gly-Pro-Trp-Pro (SEQ ID NO:27)Lys-Leu-Trp-Ile-Leu-Glu-Pro-Thr-Val-Thr-Pro-Thr (SEQ ID NO:28)Gln-Ser-Asn-Leu-Lys-Val-Ile-Pro-Ser-Trp-Trp-Phe (SEQ ID NO:29)Trp-Ile-Pro-Pro-Gln-Trp-Ser-Arg-Leu-Ile-Glu-Pro (SEQ ID NO:30)Asp-His-Pro-Gln-Ala-Lys-Pro-Asn-Trp-Tyr-Gly-Val (SEQ ID NO:31)Gly-Leu-Pro-Pro-Tyr-Ser-Pro-His-Arg-Leu-Ala-Gln (SEQ ID NO:32)Lys-Leu-Thr-Thr-Gln-Tyr-Met-Ala-Arg-Ser-Ser-Ser (SEQ ID NO:33)Lys-Val-Trp-Met-Leu-Pro-Pro-Leu-Pro-Gln-Ala-Thr (SEQ ID NO:34)Asn-Val-Thr-Ser-Thr-Ala-Phe-Ile-Asp-Thr-Pro-Trp (SEQ ID NO:35)Arg-Leu-Asn-Leu-Asp-Ile-Ile-Ala-Val-Thr-Ser-Val (SEQ ID NO:36)Thr-Leu-Pro-Ser-Pro-Leu-Ala-Leu-Leu-Thr-Val-His (SEQ ID NO:37)Thr-Asn-Arg-His-Asn-Pro-His-His-Leu-His-His-Val (SEQ ID NO:38).20. The manufacturing method according to claim 19, whereinthe base material is copper phthalocyanine and the amino acid sequence capable of binding to said base material is all or a part of Lys-Tyr-Asp-Ser-Arg-His-Leu-His-Thr-His-Ser-His (SEQ ID NO:24). 21. The manufacturing method according to claim 19, whereinthe base material is copper phthalocyanine and the amino acid sequence capable of binding to said base material is all or a part of Pro-Asn-Arg-Leu-Gly-Arg-Arg-Pro-Val-Arg-Trp-Glu (SEQ ID NO:25). 22. The manufacturing method according to claim 1, whereinthe base material is Carbon Black and the amino acid sequence capable of binding to said base material is all or a part of at least one amino acid sequence selected from the group consisting of followings: Trp-Pro-His-Ala-Trp-Lys-Val-Trp-Trp-Pro-Ala-Ser (SEQ ID NO:39)Asn-Trp-Trp-Trp-Pro-Pro-Tyr-Ile-Arg-His-Gln-Pro (SEQ ID NO:40)Trp-His-Trp-Ser-Trp-Thr-Pro-Trp-Pro-Ser-His-His (SEQ ID NO:41)Trp-Pro-Trp-Ala-Trp-His-Pro-Ser-Arg-Asp-Val-Tyr (SEQ ID NO:42)Trp-His-Gly-Tyr-Trp-Tyr-Ser-Asn-Leu-Asn-Thr-Thr (SEQ ID NO:43)Trp-Trp-Thr-Pro-Trp-Met-Ser-His-Ala-Tyr-Pro-Val (SEQ ID NO:44)Trp-Pro-Asn-Pro-Tyr-Trp-Gly-Trp-Phe-Ala-Ala-Val (SEQ ID NO:45)Thr-Ser-Trp-His-Thr-Trp-Trp-Trp-Arg-Gln-Pro-Pro (SEQ ID NO:46)Asn-Ala-Trp-His-Lys-Tyr-Trp-Trp-Pro-Ile-Thr-Lys (SEQ ID NO:47)His-Pro-Asn-Asn-Asp-Trp-Ser-Lys-Ala-Pro-Gln-Phe (SEQ ID NO:48)Trp-Trp-Thr-Pro-Gln-Pro-Trp-Trp-Ser-Phe-Pro-Ile (SEQ ID NO:49)Trp-Pro-His-Thr-Ser-Trp-Trp-Gln-Thr-Pro-Leu-Thr (SEQ ID NO:50)Trp-His-Val-Asn-Trp-Asp-Pro-Met-Ala-Trp-Tyr-Arg (SEQ ID NO:51)Ser-Trp-Pro-Trp-Trp-Thr-Ala-Tyr-Arg-Val-His-Ser (SEQ ID NO:52)Trp-His-Ser-Asn-Trp-Tyr-Gln-Ser-Ile-Pro-Gln-Val (SEQ ID NO:53)Gly-Tyr-Trp-Pro-Trp-Lys-Phe-Glu-His-Ala-Thr-Val (SEQ ID NO:54)Ala-Trp-Trp-Pro-Thr-Thr-Phe-Pro-Pro-Tyr-Tyr-Tyr (SEQ ID NO:55)Asn-Pro-Trp-Trp-Ser-His-Tyr-Tyr-Pro-Arg-Ser-Val (SEQ ID NO:56)Trp-Pro-His-Asn-Tyr-Pro-Leu-Asn-His-Ser-Asn-Pro (SEQ ID NO:57)Thr-Trp-Ala-His-Pro-Leu-Glu-Ser-Asp-Tyr-Leu-Arg (SEQ ID NO:58)His-Thr-Tyr-Tyr-His-Asp-Gly-Trp-Arg-Leu-Ala-Pro (SEQ ID NO:59)Thr-Phe-Val-Gln-Thr-Pro-Leu-Ser-His-Leu-Ile-Ala (SEQ ID NO:60)Arg-Val-Pro-Pro-Ser-Lys-Leu-Thr-Arg-Pro-Pro-Phe (SEQ ID NO:61)His-Ser-Ile-Tyr-Ser-Val-Thr-Pro-Ser-Thr-Ala-Ser (SEQ ID NO:62)Leu-Asn-Thr-Gln-Asn-His-Ala-Pro-Leu-Pro-Ser-Ile (SEQ ID NO:63).23. The manufacturing method according to claim 22, whereinthe base material is Carbon Black and the amino acid sequence capable of binding to said base material is all or a part of Trp-Pro-His-Ala-Trp-Lys-Val-Trp-Trp-Pro-Ala-Ser (SEQ ID NO:39). 24. The manufacturing method according to claim 22, whereinthe base material is Carbon Black and the amino acid sequence capable of binding to said base material is all or a part of Asn-Trp-Trp-Trp-Pro-Pro-Tyr-Ile-Arg-His-Gln-Pro (SEQ ID NO:40). 25. The manufacturing method according to claim 1, whereinthe base material is titanium oxide and the amino acid sequence capable of binding to said base material is all or a part of at least one amino acid sequence selected from the group consisting of followings: His-Ala-Thr-Gly-Thr-His-Gly-Leu-Ser-Leu-Ser-His (SEQ ID NO:150)Thr-Leu-Pro-Ser-Pro-Leu-Ala-Leu-Leu-Thr-Val-His (SEQ ID NO:151)Leu-Ser-Thr-His-Tyr-Val-Asn-Arg-Ser-His-Tle-Thr (SEQ ID NO:152)Ala-Tyr-His-Ile-Asn-Gln-Leu-Gly-Ala-Pro-Pro-Ala (SEQ ID NO:153)Leu-His-Leu-Thr-Pro-His-Pro-Gly-Asp-Thr-Leu-Thr (SEQ ID NO:154)Gln-Asp-Val-His-Leu-Thr-Gln-Gln-Ser-Arg-Tyr-Thr (SEQ ID NO:155)Leu-Glu-Ile-Pro-Ser-Asn-Gly-Leu-Asn-His-Lys-Ile (SEQ ID NO:156)Leu-Glu-Ile-Pro-Ser-Asn-Gly-Leu-Asn-His-Asn-Ile (SEQ ID NO:157).26. The manufacturing method according to claim 25, whereinthe base material is titanium oxide and the amino acid sequence capable of binding to said base material is all or a part of His-Ala-Thr-Gly-Thr-His-Gly-Leu-Ser-Leu-Ser-His (SEQ ID NO:150). 27. The manufacturing method according to claim 25, whereinthe base material is titanium oxide and the amino acid sequence capable of binding to said base material is all or a part of Thr-Leu-Pro-Ser-Pro-Leu-Ala-Leu-Leu-Thr-Val-His (SEQ ID NO:151). 28. The manufacturing method according to claim 1, whereinthe base material is a silicon board and the amino acid sequence capable of binding to said base material is Asp-Ser-His-Phe-Thr-Ile-Asn (SEQ ID NO:21). 29. The manufacturing method according to claim 1, whereinthe polyhydroxyalkanoate synthase is a polyhydroxyalkanoate synthase produced by a microbe having a capacity of production of said synthase or a transformer made by incorporating a gene associated with said capacity of production into a host. 30. The manufacturing method according to claim 29, whereinthe microbe having a capacity of production of polyhydroxyalkanoate synthase is a microbe belonging to Pseudomonas sp. 31. The manufacturing method according to claim 30, whereinthe microbe having a capacity of production of polyhydroxyalkanoate synthase is at least one microbe selected from the group consisting of Pseudomonas putida p91, FERM BP-7373; Pseudomonas cichorii H45, FERM BP-7374; Pseudomonas cichorii YN2, FERM BP-7375; and Pseudomonas jessenii P161, FERM BP-7376. 32. The manufacturing method according to claim 29, whereinthe microbe having a capacity of production of polyhydroxyalkanoate synthase is a microbe belonging to Burkholderia sp. 33. The manufacturing method according to claim 32, whereinthe microbe having a capacity of production of polyhydroxyalkanoate synthase is at least one microbe selected from the group consisting of Burkholderia cepacia KK01, FERM BP-4235; Burkholderia sp. OK3, FERM P-17370; and Burkholderia sp. OK4, FERM P-17371. 34. The manufacturing method according to claim 29, whereinthe microbe having a capacity of production of polyhydroxyalkanoate synthase is a microbe belonging to Alcaligenes sp. 35. The manufacturing method according to claim 34, whereinthe microbe having a capacity of production of polyhydroxyalkanoate synthase is Alcaligenes sp. TL2, FERM BP-6913. 36. The manufacturing method according to claim 29, whereinthe microbe having a capacity of production of polyhydroxyalkanoate synthase is a microbe belonging to Ralstonia sp. 37. The manufacturing method according to claim 36, whereinthe microbe having a capacity of production of polyhydroxyalkanoate synthase is Ralstonia eutropha TB64, FERM BP-6933. 38. The manufacturing method according to claim 29, whereinsaid host microbe of the transformer is Escherichia coli.
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이 특허에 인용된 특허 (1)
Sherman, David H.; Williams, Mark D.; Xue, Yongquan, Metabolic engineering of polyhydroxyalkanoate monomer synthases.
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