초록 ▼

Method for isolating DNA contained in a biological sample. The method includes combining in a solution a DNA-containing biological sample, a salt, a cationic surfactant, and a DNA-binding carrier, the solution having a salt concentration higher than the DNA precipitation inhibition-initiating concen

Method for isolating DNA contained in a biological sample. The method includes combining in a solution a DNA-containing biological sample, a salt, a cationic surfactant, and a DNA-binding carrier, the solution having a salt concentration higher than the DNA precipitation inhibition-initiating concentration, to lyse the DNA-containing biological sample and to bind DNA to the DNA-binding carrier while in the solution to form a bound DNA-carrier. The method also includes separating the DNA-bound carrier from other components. The method further includes dissociating the bound DNA from the DNA-binding carrier. The method still further includes recovering dissociated DNA.

대표청구항 ▼

1. A method for isolating DNA contained in a biological sample, comprising:combining in a solution a DNA-containing biological sample, a salt, a cationic surfactant, and a DNA-binding carrier which is not hydroxyapatite, the solution having a salt concentration higher than the DNA precipitation inhi

1. A method for isolating DNA contained in a biological sample, comprising:combining in a solution a DNA-containing biological sample, a salt, a cationic surfactant, and a DNA-binding carrier which is not hydroxyapatite, the solution having a salt concentration higher than the DNA precipitation inhibition-initiating concentration, to lyse the DNA-containing biological sample and to bind DNA to the DNA-binding carrier while in the solution of the DNA-containing biological sample, the salt, the cationic surfactant, and the DNA-binding carrier to form a bound DNA-carrier; separating the DNA-bound carrier from other components; dissociating the bound DNA from the DNA-binding carrier; and recovering dissociated DNA. 2. The method of claim 1, wherein the DNA-containing biological sample comprises blood.3. The method of claim 1, wherein the DNA-containing biological sample comprises a whole biological sample.4. The method of claim 1, wherein the DNA-containing biological sample is not pretreated by centrifugation.5. The method of claim 1, wherein the dissociation of the bound DNA is conducted without phosphate.6. The method of claim 1, wherein the DNA-containing biological sample comprises a cell.7. The method of claim 1, wherein the DNA-containing biological sample comprises a biological tissue.8. A method for isolating DNA contained in a biological sample, comprising:including a salt, a cationic surfactant, and a DNA-binding carrier which is not hydroxyapatite in a solution; including a DNA-containing biological sample in the solution of the salt, the cationic surfactant and the DNA-binding carrier so that the solution has a salt concentration higher than the DNA precipitation inhibition-initiating concentration so as to lyse the DNA-containing biological sample and to bind DNA to the DNA-binding carrier while in the solution of the DNA-containing biological sample, the salt, the cationic surfactant, and the DNA-binding carrier to form a bound DNA-carrier; separating the DNA-bound carrier from other components; dissociating the bound DNA from the DNA-binding carrier; and recovering dissociated DNA. 9. The method of claim 8, wherein the DNA-containing biological sample comprises blood.10. The method of claim 8, wherein the DNA-containing biological sample comprises a whole biological sample.11. The method of claim 8, wherein the DNA-containing biological sample is not pretreated by centrifugation.12. The method of claim 8, wherein the dissociation of the bound DNA is conducted without phosphate.13. The method of claim 8, wherein the DNA-containing biological sample comprises a cell.14. The method of claim 8, wherein the DNA-containing biological sample comprises a biological tissue.