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The invention provides methods for isolating and releasing genetic material from a solid medium, such as a DNA array, a filter, a card, or a multiple-well plate, using a wash solution comprising a photolytic agent, followed by subsequent exposure to a light source to release the genetic material, su

The invention provides methods for isolating and releasing genetic material from a solid medium, such as a DNA array, a filter, a card, or a multiple-well plate, using a wash solution comprising a photolytic agent, followed by subsequent exposure to a light source to release the genetic material, such as genomic DNA. The invention also includes devices and kits for practicing these methods.

대표청구항 ▼

1. A method for releasing genetic material from a solid medium, wherein the method comprises:a. providing a solid medium comprising a matrix comprising genetic material;b. washing the solid medium, under conditions of limited light or in the dark, with a wash solution comprising:i. a buffer; andii.

1. A method for releasing genetic material from a solid medium, wherein the method comprises:a. providing a solid medium comprising a matrix comprising genetic material;b. washing the solid medium, under conditions of limited light or in the dark, with a wash solution comprising:i. a buffer; andii. a photolytic agent;c. exposing the washed solid medium to a light source to release the genetic material; andd. eluting the released genetic material from the solid medium.2. The method of claim 1, wherein the genetic material comprises DNA.3. The method of claim 2, wherein the genetic material comprises genomic DNA.4. The method of claim 1, wherein the wash solution of step b further comprises a co-releasing agent.5. The method of claim 1, wherein the solid medium comprises a DNA array or a multiple-well plate.6. The method of claim 1, wherein the light source is selected from the group consisting of an incandescent lamp, a fluorescent lamp, an ultraviolet lamp, a heat lamp, a quartz lamp, a laser beam, and combinations thereof.7. The method of claim 1, wherein the buffer has a pH range between 6.0 and 10.0.8. The method of claim 1, wherein the photolytic agent is selected from the group consisting of hematoporphyrin, imidazole, methylene blue, riboflavin, ethidium, EDTA, polyacrylate, polyvinyl sulfate, and combinations thereof.9. The method of claim 4, wherein the co-releasing agent is selected from the group consisting of sodium dodecyl sulfate, sodium lauryl sulfate, alkyl aryl sulfonates, long chain (fatty) alcohol sulfates, olefine sulfates, sulfosuccinates, phosphate esters, sodium 2-ethylhexysulfate, polyvinyl sulfate, polyacrylate, polyphosphate, sodium polyacrylate, sodium polyvinyl sulfate, and combinations thereof.10. A method for isolating genetic material from a biological sample, wherein the method comprises:a. applying the biological sample comprising genetic material to a solid medium comprising a matrix;b. retaining the genetic material with the solid medium;c. washing the solid medium, under conditions of limited light or in the dark, with a wash solution comprising:i. a buffer; andii. a photolytic agent;d. removing at least a portion of the non-genetic components of the biological sample from the solid medium;e. exposing the washed solid medium to a light source to release the genetic material; andf. eluting the released genetic material from the solid medium.11. The method of claim 10, wherein the genetic material comprises DNA.12. The method of claim 11, wherein the genetic material comprises genomic DNA.13. The method of claim 10, wherein the wash solution of step c further comprises a co-releasing agent.14. The method of claim 10, wherein the solid medium comprises a DNA array or a multiple-well plate.15. The method of claim 10, wherein the light source is selected from the group consisting of an incandescent lamp, a fluorescent lamp, an ultraviolet lamp, a heat lamp, a quartz lamp, a laser beam, and combinations thereof.16. The method of claim 10, wherein the buffer has a pH range between 6.0 and 10.0.17. The method of claim 10, wherein the photolytic agent is selected from the group consisting of hematoporphyrin, imidazole, methylene blue, riboflavin, ethidium, EDTA, polyacrylate, polyvinyl sulfate, and combinations thereof.18. The method of claim 13, wherein the co-releasing agent is selected from the group consisting of sodium dodecyl sulfate, sodium lauryl sulfate, alkyl aryl sulfonates, long chain (fatty) alcohol sulfates, olefine sulfates, sulfosuccinates, phosphate esters, sodium 2-ethylhexysulfate, polyvinyl sulfate, polyacrylate, polyphosphate, sodium polyacrylate, sodium polyvinyl sulfate, and combinations thereof.19. A method for isolating genetic material from a biological sample, wherein the method comprises:a. providing a dry solid medium comprising a matrix, having a composition sorbed thereto, wherein the composition comprises:i. a weak base;ii. a chelating agent; andiii. an anionic detergent or surfactant;b. applying the biological sample comprising genetic material to the solid medium;c. retaining the genetic material with the solid medium;d. washing the solid medium, under conditions of limited light or in the dark, with a wash solution comprising:i. a buffer;ii. a co-releasing agent; andiii. a photolytic agent;e. removing at least a portion of the non-genetic components of the biological sample from the solid medium;f. exposing the washed solid medium to a light source to release the genetic material; andg. eluting the released genetic material from the solid medium.20. The method of claim 19, wherein the genetic material comprises DNA.21. The method of claim 20, wherein the genetic material comprises genomic DNA.22. The method of claim 19, wherein the solid medium comprises a DNA array or a multiple-well plate.23. The method of claim 19, wherein the light source is selected from the group consisting of an incandescent lamp, a fluorescent lamp, an ultraviolet lamp, a heat lamp, a quartz lamp, a laser beam, and combinations thereof.24. The method of claim 19, wherein the buffer has a pH range between 6.0 and 10.0.25. The method of claim 19, wherein the photolytic agent is selected from the group consisting of hematoporphyrin, imidazole, methylene blue, riboflavin, ethidium, EDTA, polyacrylate, polyvinyl sulfate, and combinations thereof.26. The method of claim 19, wherein the co-releasing agent is selected from the group consisting of sodium dodecyl sulfate, sodium lauryl sulfate, alkyl aryl sulfonates, long chain (fatty) alcohol sulfates, olefine sulfates, sulfosuccinates, phosphate esters, sodium 2-ethylhexysulfate, polyvinyl sulfate, polyacrylate, polyphosphate, sodium polyacrylate, sodium polyvinyl sulfate, and combinations thereof.27. A device for isolating genetic material from a biological sample, wherein the device comprises:a. a solid medium comprising a matrix;b. a composition sorbed to the matrix, wherein the composition comprises:i. a weak base;ii. a chelating agent; andiii. an anionic detergent or surfactant;c. a covering capable of limiting exposure of the solid matrix to light; andd. a light source capable of activating a photolytic agent to release genetic material from the solid matrix.28. The device of claim 27, wherein the light source is selected from the group consisting of an incandescent lamp, a fluorescent lamp, an ultraviolet lamp, a heat lamp, a quartz lamp, a laser beam, and combinations thereof.29. A device for isolating genetic material from a biological sample, wherein the device comprises:a. a DNA array or a multiple-well plate;b. a covering capable of limiting exposure of the solid matrix to light; andc. a light source capable of activating a photolytic agent to release genetic material from the solid matrix.30. The device of claim 29, wherein the light source is selected from the group consisting of an incandescent lamp, a fluorescent lamp, an ultraviolet lamp, a heat lamp, a quartz lamp, a laser beam, and combinations thereof.31. A kit for isolating genetic material, wherein the kit comprises:a. a solid medium comprising a matrix;b. a composition sorbed to the matrix, wherein the composition comprises:i. a weak base;ii. a chelating agent; andiii. an anionic detergent or surfactant; andc. a wash solution comprising:i. a buffer; andii. a photolytic agent.32. The kit of claim 31, wherein the wash solution of part c further comprises a co-releasing agent.33. The kit of claim 31, wherein the buffer has a pH range between 6.0 and 10.0.34. The kit of claim 31, wherein the photolytic agent is selected from the group consisting of hematoporphyrin, imidazole, methylene blue, riboflavin, ethidium, EDTA, polyacrylate, polyvinyl sulfate, and combinations thereof.35. The kit of claim 32, wherein the co-releasing agent is selected from the group consisting of sodium dodecyl sulfate, sodium lauryl sulfate, alkyl aryl sulfonates, long chain (fatty) alcohol sulfates, olefine sulfates, sulfosuccinates, phosphate esters, sodium 2-ethylhexysulfate, polyvinyl sulfate, polyacrylate, polyphosphate, sodium polyacrylate, sodium polyvinyl sulfate, and combinations thereof.36. A kit for isolating genetic material, wherein the kit comprises:a. a DNA array or multiple-well plate, wherein the DNA array or multiple-well plate comprises a dry solid medium comprising:i. a matrix; andii. a composition sorbed to the matrix, wherein the composition comprises an anionic surfactant or detergent; andb. a wash solution comprising:i. a buffer; andii. a photolytic agent.37. The kit of claim 36, wherein the wash solution of part b further comprises a co-releasing agent.38. The kit of claim 36, wherein the buffer has a pH range between 6.0 and 10.0.39. The kit of claim 36, wherein the photolytic agent is selected from the group consisting of hematoporphyrin, imidazole, methylene blue, riboflavin, ethidium, EDTA, polyacrylate, polyvinyl sulfate, and combinations thereof.40. The kit of claim 37, wherein the co-releasing agent is selected from the group consisting of sodium dodecyl sulfate, sodium lauryl sulfate, alkyl aryl sulfonates, long chain (fatty) alcohol sulfates, olefine sulfates, sulfosuccinates, phosphate esters, sodium 2-ethylhexysulfate, polyvinyl sulfate, polyacrylate, polyphosphate, sodium polyacrylate, sodium polyvinyl sulfate, and combinations thereof.41. The method of claim 1, wherein the solid medium further comprises a dry solid medium comprising a composition sorbed to the matrix, wherein the composition comprises an anionic surfactant or detergent.42. The method of claim 41, wherein the composition further comprises:a. a weak base; andb. a chelating agent.43. The method of claim 42, wherein:a. the weak base comprises a Tris base;b. the chelating agent comprises ethylenediamine tetra-acetic acid (EDTA); andc. the anionic surfactant or detergent comprises sodium dodecyl sulfate (SDS).44. The method of claim 10, wherein the solid medium comprises a dry solid medium further comprising a composition sorbed to the matrix, wherein the composition comprises an anionic surfactant or detergent.45. The method of claim 44, wherein the composition further comprises:a. a weak base; andb. a chelating agent.46. The method of claim 45, wherein:a. the weak base comprises a Tris base;b. the chelating agent comprises ethylenediamine tetra-acetic acid (EDTA); andc. the anionic surfactant or detergent comprises sodium dodecyl sulfate (SDS).47. The device of claim 29, wherein the wherein the DNA array or multiple-well plate comprises a dry solid medium comprisingi. a matrix; andii. a composition sorbed to the matrix, wherein the composition comprises an anionic surfactant or detergent.48. The device of claim 47, wherein the composition further comprises:a weak base; anda chelating agent.49. The device of claim 48, wherein:a. the weak base comprises a Tris base;b. the chelating agent comprises ethylenediamine tetra-acetic acid (EDTA); andc. the anionic surfactant or detergent comprises sodium dodecyl sulfate (SDS).50. The kit of claim 35, wherein the composition further comprises:a. a weak base; andb. a chelating agent.51. The kit of claim 50, wherein:a. the weak base comprises a Tris base;b. the chelating agent comprises ethylenediamine tetra-acetic acid (EDTA); andc. the anionic surfactant or detergent comprises sodium dodecyl sulfate (SDS).