Method and apparatus for concurrently detecting pathogenic organisms and antimicrobial susceptibility
원문보기
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
C12Q-001/04
C12Q-001/18
출원번호
US-0942369
(1997-10-02)
발명자
/ 주소
Chen,Chun Ming
Carpenter,Charles R.
Gu,Haoyi
Naqui,Ali
출원인 / 주소
IDEXX Laboratories, Inc.
대리인 / 주소
McDonnell Boehnen Hulbert &
인용정보
피인용 횟수 :
3인용 특허 :
14
초록▼
The present invention relates to a method of detecting the presence of target microorganisms in a biological sample and of simultaneously determining the susceptibility of the microorganisms to antimicrobial agents. The target microbial organisms may be urinary pathogens. The methods include the st
The present invention relates to a method of detecting the presence of target microorganisms in a biological sample and of simultaneously determining the susceptibility of the microorganisms to antimicrobial agents. The target microbial organisms may be urinary pathogens. The methods include the steps of providing a multicompartment assay device with at least one compartment containing a medium capable of sustaining the growth of total viable microorganisms, at least one compartment containing a medium capable of sustaining the growth of target microorganisms, and at least one compartment containing an antimicrobial susceptibility interpretation medium. A biological sample is placed in each compartment and the presence and antimicrobial susceptibility of the target microorganisms which may be present is determined by analyzing which compartments exhibit microbial growth.
대표청구항▼
What is claimed is: 1. A method of detecting the presence or urinary pathogens in a biological sample and of simultancously determining the susceptibility of the urinary pathogens to antimicrobial agents, said method comprising: providing a multicompartment assay device comprising: at least one com
What is claimed is: 1. A method of detecting the presence or urinary pathogens in a biological sample and of simultancously determining the susceptibility of the urinary pathogens to antimicrobial agents, said method comprising: providing a multicompartment assay device comprising: at least one compartment comprising a medium capable of sustaining growth of total microbial organisms; at least one compartment comprising a uropathogenic specific medium; and, at least one compartment comprising an antimicrobial susceptibility interpretation medium; placing a portion of the biological sample respectively in said at least one compartment comprising a medium capable of sustaining growth of total microbial organisms; said at least one compartment comprising a uropathogenic specific medium; and, said at least one compartment comprising an antimicrobial susceptibility interpretation medium comprising an antimicrobial agent; whereby metabolism of a signal generating substrate and production of a detectable signal in said at least one compartment comprising a medium capable of sustaining growth of total microbial organisms indicates the presence of microbial organisms in the sample; metabolism of a signal generating substrate and production of a detectable signal in said at least one compartment comprising a uropathogenic specific medium indicates the presence of urinary pathogens in the sample; and metabolism of a signal generating substrate and production of a detectable signal in said at least one compartment comprising an antimicrobial susceptibility interpretation medium indicates that the organisms lack susceptibility to the antimicrobial agent comprised in said antimicrobial susceptibility interpretation medium; and examining the compartments to determine the presence of urinary pathogens in said biological sample and the susceptibility of said urinary pathogens to said antimicrobial agents. 2. The method of claim 1, wherein the biological fluid is urine. 3. The method of claim 2, wherein the urinary pathogens are primary gram negative urinary pathogens. 4. The method of claim 3 wherein the primary gram negative urinary pathogens comprise Enterobacteriacae. 5. The method of claim 3 wherein the primary gram negative urinary pathogens are selected from the group consisting of: Escherichia coli, Klebsiella spp., Enterobacter spp., Proteus mirabilis Proteus vulgaris, Morganella morganii, Providencia retteri, and Acinetobacter spp. 6. The method of claim 1 wherein the at least one antimicrobial susceptibility interpretation medium comprises amoxicillin, clavulanic acid/amoxicillin, or enrofloxacin. 7. The method of claim 1 wherein the signal generating substrate is fluorogenic or chromogenic. 8. A method of detecting the presence of urinary pathogens in a biological sample and of simultaneously determining the susceptibility of the urinary pathogens to antimicrobial agents, said method comprising: providing a multicompartment assay device comprising: at least one compartment comprising a medium capable of sustaining growth of total microbial organisms; at least one compartment comprising a uropathogenic specific medium comprising a methyl-umbelliferyl substrate; and, at least one compartment comprising an antimicrobial susceptibility interpretation medium; placing a portion of the biological sample respectively in said at least one compartment comprising a medium capable of sustaining growth of total microbial organisms; said at least one compartment comprising a uropathogenic specific medium comprising a methyl-umbelliferyl substrate; and, said at least one compartment comprising an antimicrobial susceptibility interpretation medium comprising an antimicrobial agent; whereby metabolism of a signal generating substrate and production of a detectable signal in said at least one compartment comprising a medium capable of sustaining growth of total microbial organisms indicates the presence of microbial organisms in the sample; metabolism of a methyl-umbelliferyl signal generating substrate and production of a detectable signal in said at least one compartment comprising a uropathogenic specific medium indicates the presence of urinary pathogens in the sample; and metabolism of a signal generating substrate and production of a detectable signal in said at least one compartment comprising an antimicrobial susceptibility interpretation medium indicates that the organisms lack susceptibility to the antimicrobial agent comprised in said antimicrobial susceptibility interpretation medium; and examining the compartments to determine the presence of urinary pathogens in said biological sample and the susceptibility of said urinary pathogens to said antimicrobial agents. 9. The method of claim 8, wherein the biological fluid is urine. 10. The method of claim 9, wherein the urinary pathogens are primary gram negative urinary pathogens. 11. The method of claim 10 wherein the primary gram negative urinary pathogens comprise Enterobacteriacae. 12. The method of claim 10 wherein the primary gram negative urinary pathogens are selected from the group consisting of: Escherichia coli, Klebsiella spp., Enterobacter spp., Proteus mirabilis, Proteus vulgaris, Morganella morgani, Providencia retteri, and Acinetobacter spp. 13. The method of claim 8 wherein the at least one antimicrobial susceptibility interpretation medium comprises amoxicillin, clavulanic acid/amoxicillin, or enrofloxacin. 14. A method of detecting the presence of urinary pathogens in a biological sample and of simultaneously determining the susceptibility of the urinary pathogens to antimicrobial agents, said method comprising: providing a multicompartment assay device comprising: at least one compartment comprising a medium capable of sustaining growth of total microbial organisms; at least one compartment comprising a uropathogenic specific medium comprising yeast extract; and, at least one compartment comprising an antimicrobial susceptibility interpretation medium; placing a portion of the biological sample respectively in said at least one compartment comprising a medium capable of sustaining growth of total microbial organisms; said at least one compartment comprising a uropathogenic specific medium comprising yeast extract; and, said at least one compartment comprising an antimicrobial susceptibility interpretation medium comprising an antimicrobial agent: whereby metabolism of a signal generating substrate and production of a detectable signal in said at least one compartment comprising a medium capable of sustaining growth of total microbial organisms indicates the presence of microbial organisms in the sample; metabolism of a signal generating substrate and production of a detectable signal in said at least one compartment comprising a uropathogenic specific medium comprising yeast extract indicates the presence of urinary pathogens in the sample; and metabolism of a signal generating substrate and production of a detectable signal in said at least one compartment comprising an antimicrobial susceptibility interpretation medium indicates that the organisms lack susceptibility to the antimicrobial agent comprised in said antimicrobial susceptibility interpretation medium; and examining the compartments to determine the presence of urinary pathogens in said biological sample and the susceptibility of said urinary pathogens to said antimicrobial agents. 15. The method of claim 14, wherein the biological fluid is urine. 16. The method of claim 15, wherein the urinary pathogens are primary gram negative urinary pathogens. 17. The method of claim 16 wherein the primary gram negative urinary pathogens comprise Enterobacteriacae. 18. The method of claim 16 wherein the primary gram negative urinary pathogens are selected from the group consisting of: Escherichia coli, Klebsiella spp., Enterobacter spp., Proteus mirabilis, Proteus vulgaris, Morganella morganii, Providencia retteri, and Acinetobacter spp. 19. The method of claim 14 wherein the at least one antimicrobial susceptibility interpretation medium comprises amoxicillin, clavulanic acid/amoxicillin, or enrofloxacin.
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이 특허에 인용된 특허 (14)
Himmler Thomas (Odenthal DEX) Petersen Uwe (Leverkusen DEX) Bremm Klaus-Dieter (Recklinghausen DEX) Endermann Rainer (Wuppertal DEX) Stegemann Michael (Leverkusen DEX) Wetzstein Heinz-Georg (Leverkus, 5-vinyl- and 5-ethinyl-quinolone- carboxylic acids.
Matsumura Paul M. ; Hyman Jones M. ; Jeffrey Scott R. ; Maresch Martin J. ; Thorpe Thurman C. ; Barron William G., Apparatus and method for detecting, quantifying and characterizing microorganisms.
Gibson Sandra F. (St. Louis County MO) Fadler Norman L. (St. Peters MO), Apparatus and process for determining the susceptibility of microorganisms to antibiotics.
Sussman Mark L. (Baltimore MD) Wilson Stephen G. (South Bridge MA) Tice Gregory (Lutherville MD), Device for enhancing fluorescence and kinetics and methods of using the device.
Arvesen James N. (Princeton NJ), Method and apparatus for determining the minimum concentration of antibiotic necessary to at least inhibit microorganism.
Badal Robert (Sacramento CA) Kelley Roger (Kansas City MO) Sand Theodore T. (Poway CA) Bascomb Shoshana (Davis CA), Method and composition for determining antimicrobial susceptibility of the majority clinically significant Gram postitiv.
Edberg Stephen C. (356 Woodland La. Orange CT 06477), Method and medium for use in detecting target microbes in situ in a specimen sample of a possibly contaminated material.
Carr Anthony H. (28 Manor Farm Way Sharnbrook ; Bedford GB3) Badley Robert A. (19 Well Pond Close Sharnbrook ; Bedford GB3) Jobling Ian (25 Lynford Way Rushden ; Northamptonshire GB3) Sands Thomas J., Microbial susceptibility assay using 7-N-(aminoacyl)-7-amido-4-methylcoumarins.
Chen,Chun Ming; Carpenter,Charles; Gu,Haoyi; Naqui,Ali; Madsen,Gary; O'Connor, Jr.,Thomas; Esty,Katherine Jean, Methods and devices for the detection of pathogenic microorganisms and their antimicrobial susceptibility.
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