Broad-range light-detection systems, including components and methods of use thereof. These systems may include apparatus and methods for detecting light with increased speed and/or detection efficiency, particularly in applications involving repeated analysis of the same sample and/or successive an
Broad-range light-detection systems, including components and methods of use thereof. These systems may include apparatus and methods for detecting light with increased speed and/or detection efficiency, particularly in applications involving repeated analysis of the same sample and/or successive analysis of different samples, and particularly when the sample or samples have a wide range of light intensities. These systems also may include apparatus and methods for detecting light with increased accuracy over a broad range of intensities. These systems also may include vapparatus and methods for automatically scaling detection range to improve detection based on the intensity of the detected light.
대표청구항▼
We claim: 1. A light detection device, comprising: a detector configured to receive light from a sample; an accumulator configured to generate an accumulator signal corresponding to the total light received by the detector from the sample during a sampling period; a timer configured to measure elap
We claim: 1. A light detection device, comprising: a detector configured to receive light from a sample; an accumulator configured to generate an accumulator signal corresponding to the total light received by the detector from the sample during a sampling period; a timer configured to measure elapsed time during the sampling period; and a controller configured to terminate the sampling period after the accumulator signal reaches a predetermined accumulator value or after the elapsed time reaches a predetermined detection time limit, whichever occurs first. 2. The device of claim 1, wherein a target measurement precision is used to calculate the predetermined accumulator value. 3. The device of claim 2, wherein a model of measurement noise further is used to calculate the predetermined accumulator value. 4. The device of claim 3, wherein the model of measurement noise is shot noise. 5. The device of claim 2, wherein an estimated measurement result further is used to calculate the predetermined accumulator value. 6. The device of claim 5, wherein the estimated measurement result is calculated using light received from the sample prior to or during the sampling period. 7. The device of claim 1, wherein the device is configured to receive light automatically and sequentially from a plurality of samples. 8. The device of claim 7, wherein the plurality of samples are disposed at different positions in a microplate or a microarray. 9. The device of claim 7 further comprising an automatic sample handler configured to advance samples for analysis, wherein the time between beginning to analyze successive samples is shorter if the accumulator signal reaches the predetermined accumulator value before the elapsed time reaches the predetermined detection time limit. 10. The device of claim 7, wherein the device is configured to receive light automatically from each of the plurality of samples during first and second sampling periods. 11. The device of claim 7, wherein the predetermined detection time limit is calculated using a user-inputted maximum total time for receiving light from the plurality of samples. 12. The device of claim 1, wherein the device is configured to receive light from the sample automatically during first and second sampling periods. 13. The device of claim 12, wherein the first and second sampling periods are sequential. 14. The device of claim 12, wherein the first and second sampling periods are at least partially overlapping. 15. The device of claim 12, wherein the controller is configured to terminate the first and second sampling periods independently after an accumulator signal for each period reaches a predetermined accumulator value or after the elapsed time for each period reaches a predetermined detection time limit, whichever occurs first. 16. The device of claim 12, wherein the duration of the second sampling period is determined at least in part by the duration of the first sampling period. 17. The device of claim 16, wherein the controller is configured to terminate the second sampling period such that it has at least substantially the same duration as the first sampling period. 18. The device of claim 16, wherein the controller is configured to terminate the second sampling period such that it has a duration equal to about twice the predetermined detection time limit minus the duration of the first sampling period. 19. The device of claim 12, wherein the device is configured such that the light received during the first and second sampling periods is of at least substantially the same wavelength. 20. The device of claim 12, wherein the device is configured such that the light received during the first and second sampling periods is of at least substantially different wavelengths. 21. The device of claim 12, wherein the device is configured such that the light received during the first and second sampling periods is of at least substantially different polarization. 22. The device of claim 12, further comprising a processor configured to compute a quantity related to the polarization of the light received during the first and second sampling periods. 23. The device of claim. 22, wherein the quantity is a luminescence polarization or a luminescence anisotropy. 24. The device of claim 12 further comprising a processor configured to compute a quantity related to resonance energy transfer efficiency in the sample based on the light received during the first and second sampling periods. 25. The device of claim 12 further comprising a processor configured to compute a quantity related to a ratio of the light received during the first and second periods. 26. The device of claim 1 further comprising an excitation source configured to excite emission of light from the sample. 27. The device of claim 26, wherein the excitation source is a light source. 28. The device of claim 26, wherein the excitation source is an electrical potential or current. 29. The device of claim 1, the detector, accumulator, accumulator signal, and sampling period being a first detector, a first accumulator, a first accumulator signal, and a first sampling period, respectively, further comprising: a second detector configured to receive light from the sample; and a second accumulator configured to generate a second accumulator signal corresponding to the total light received by the second detector from the sample during a second sampling period. 30. The device of claim 1, wherein the accumulator is discrete and generates the accumulator signal by counting pulses from the detector corresponding to quanta of detected light. 31. The device of claim 1, wherein the accumulator is analog and generates the accumulator signal by charging an integration capacitor, the charge on the capacitor corresponding to the amount of detected light. 32. The device of claim 31, the accumulator including a plurality of integration capacitors having substantially different capacities from one other, wherein the amount of detected light required to generate an accumulator signal equal to the predetermined accumulator value is selectable by choosing a particular one of the integration capacitors. 33. The device of claim 31, the accumulator also including a discrete accumulator that accumulates the signal by counting pulses from the detector corresponding to quanta of detected light, wherein the device switches between the discrete and analog accumulators based on the amount of light detected. 34. The device of claim 1, wherein the accumulator is digital and generates the accumulator signal by digitally measuring and summing the output of the detector at a plurality of discrete times during the sampling period. 35. The device of claim 1 further including a plurality of accumulators simultaneously operatively connected in parallel to the detector, each accumulator being configured to generate an integration value proportional to the integrated output of the detector during the sampling period, wherein the proportionality between the accumulator signal and the amount of detected light is substantially different for the different accumulators. 36. The device of claim 1, wherein the detector is selected from a group consisting of photomultiplier tubes, photodiodes, avalanche photodiodes, and charge-coupled devices. 37. The device of claim 1, wherein the device is adapted to switch between digital and analog counting modes, depending on the amount of detected light. 38. The device of claim 1, wherein the predetermined detection time limit is measured by pulses of a flash lamp. 39. A method of detecting light from a sample, comprising: detecting photons incident on a detector during a sampling period; collecting data representative of the cumulative number of photons detected during the sampling period; and terminating the sampling period when the cumulative number of photons detected during the sampling period reaches a predetermined threshold or a predetermined sample time expires, whichever occurs first. 40. The method of claim 39, further comprising calculating the predetermined threshold based on a target measurement precision. 41. The method of claim 39, further comprising repeating the steps of detecting, collecting, and terminating for the same sample. 42. The method of claim 41, further comprising computing a polarization based on the detected light. 43. The method of claim 41, further comprising computing a resonance energy transfer efficiency based on the detected light. 44. The method of claim 41, further comprising computing a ratio based on the detected light. 45. The method of claim 39, further comprising repeating the steps of detecting, collecting, and terminating for a second sample. 46. A light detection device, comprising: means for receiving light from a sample; means for generating an accumulator signal corresponding to the total light received by the detector from the sample during a sampling period; means for measuring elapsed time during the sampling period; and means for terminating the sampling period after the accumulator signal reaches a predetermined accumulator value or after the elapsed time reaches a predetermined detection time limit, whichever occurs first.
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d\Auria Luigi (Paris FRX), Adjustable distributor device for shared transmission of radiant energy.
Stavrianopoulos Jannis (New York NY) Rabbani Elazar (New York NY) Abrams Samuel B. (New York NY) Wetmur James G. (Scardsdale NY), Analyte detection by means of energy transfer.
Herron James N. (Salt Lake City UT) Christensen Douglas A. (Salt Lake City UT) Wang Hsu-Kun (Salt Lake City UT) Caldwell Karin D. (Salt Lake City UT) Janatova Vera (Prague CSX) Huang Shao-Chie (Salt , Apparatus and methods for multi-analyte homogeneous fluoro-immunoassays.
Massey Richard J. ; Blackburn Gary F. ; Wilkins Elizabeth W. ; Shah Haresh P., Apparatus for improved luminescence assays using particle concentration and chemiluminescence detection.
Massey Richard J. ; Blackburn Gary F. ; Wilkins Elizabeth W. ; Leland Jonathan K., Apparatus for improved luminescence assays using particle concentration, electrochemical generation of chemiluminescence.
De Maeyer ; Leo C. M. ; Rabl ; Carl-Roland ; Jovin ; Thomas M. ; Riesne r ; Detlev ; Rigler ; Rudolf ; Veil ; Lutz Bodo ; Lehrach ; Hans, Apparatus for investigating fast chemical reactions by optical detection.
Cercek Boris (4318 Camphor Ave. Yorba Linda CA 92686) Cercek Lea (4318 Camphor Ave. Yorba Linda CA 92686), Apparatus for measuring polarization of bathochromically shifted fluorescence.
Zoha Steven J. (Jarrettsville MD) Davis James E. (Wilmington) Craig Alan R. (Wilmington DE) Hochberg Alan M. (Hockessin DE), Apparatus for performing fluorescent assays which separates bulk and evanescent fluorescence.
Aslund Nils R. D. (Skontorpsvagen 126 ; 9 tr S-121 65 Johanneshov ; Stockholm SEX) Carlsson Kjell S. (Malmbodavagen 17 S-186 42 Vallentuna SEX), Apparatus for quantitative imaging of multiple fluorophores using dual detectors.
Ullman Edwin F. (Atherton CA) Kirakossian Hrair (San Jose CA) Pease John S. (Los Altos CA) Daniloff Yuri (Mountain View CA) Wagner Daniel B. (Sunnyvale CA), Assay method utilizing photoactivated chemiluminescent label.
Akong Michael Anthony (San Diego CA) Harpold Michael Miller (El Cajon CA) Velicelebi Gonul (San Diego CA) Brust Paul (San Diego CA), Automated analysis equipment and assay method for detecting cell surface protein and/or cytoplasmic receptor function us.
Pfoutz Ray W. (Sunnyvale CA) Mauritz Karl H. (Santa Clara CA) Skurla John P. (Los Gatos CA), Automatic exposure control for a luminous object monitor system.
Baetge Edward E. (Barrington RI) Hammang Joseph P. (Barrington RI) Gentile Frank T. (Warwick RI) Lindner Mark D. (Bristol RI) Winn Shelley R. (Smithfield RI) Emerich Dwaine F. (Providence RI), Compositions and methods for the delivery of biologically active molecules using genetically altered cells contained in.
Hui Raymond A. (Lyndhurst NJ) Schwenzer Kathryn S. (Yardley PA), Compounds having improved fluorescence in fluorescence polarization immunoassays and immunoassays utilizing same.
Hafeman Dean G. (Hillsborough CA) Parce John W. (Palo Alto CA) McConnell Harden M. (Stanford CA), Device for photoresponsive detection and discrimination.
Hafeman Dean (San Bruno CA) Parce John W. (Winston-Salem NC) McConnell Harden M. (Stanford CA), Device having photoresponsive electrode for determining analytes including ligands and antibodies.
Fletcher ; James C. Administrator of the National Aeronautics and Space ; Administration ; with respect to an invention of ; Shumate ; Michael S., Differential optoacoustic absorption detector.
Kikinis Dan (Saratoga CA) Dornier Pascal (Sunnyvale CA) Seiler William J. (Scotts Valley CA), Digital assistant system having a host computer with a docking bay and a moveable heat sink for cooling a docked module.
Walker G. Terrance (Chapel Hill NC) Nadeau James G. (Chapel Hill NC) Linn C. Preston (Durham NC), Fluorerscence polarization detection of nucleic acid amplication.
Linn Carl Preston (Durham NC) Walker G. Terrance (Chapel Hill NC) Spears Patricia Anne (Raleigh NC), Fluorescence polarization detection of nucleic acids.
Berndt Klaus W. (Baltimore) Gryczynski Ignacy (Baltimore) Lakowicz Joseph R. (Columbia MD), Fluorometry method and apparatus using a semiconductor laser diode as a light source.
Brooker Gary (9212 Bentridge Ave. Potomac MD 20854), High-speed multiple wavelength illumination source, apparatus containing the same, and applications thereof to methods o.
Modlin Douglas N. ; Edwards Glenn R. ; Taylor Michael T. ; Marquiss Samuel A. ; El-Hage Amer ; Barker Craig S. ; Bechtel Lorne B. ; Stellmacher Rick V. ; Granieri ; Jr. Philip A. ; Lembi ; Sr. Robert, Light detection device having an optical-path switching mechanism.
Chow Herbert S. (Palatine IL) Kotlarik John J. (McHenry IL) Wroblewski Mieczyslaw (Lake Forest IL) Wilson Thomas J. (Antioch IL) McCoy Jimmy D. (Keller TX), Liquid level sensing method and device.
James Douglas R. (London CAX) Ware William R. (London CAX), Luminescence system and method for determining the nature of substances by measuring fluorescence and phosphorescence pr.
Andreotti Peter E. (Lauderhill FL) Morse Irwin S. (Miami FL) Thornthwaite Jerry T. (Miami FL) Heimer Malcolm L. (Miami FL) Salinger Jorge D. (Miami FL) Sobodowski Joseph J. (Miami FL), Luminometer.
Baba Shigeo (3-31-8 ; Hirayama Hino-shi ; Tokyo JPX), Method and apparatus for determining b
상세보기
Lakowicz Joseph R. (9142 Emersons Reach Columbia MD 21045) Berndt Klaus W. (Baltimore MD) Nowaczyk Kazimierz (Baltimore MD) Szmacinski Henryk (Baltimore MD) Johnson Michael L. (Charlottesville VA), Method and apparatus for multi-dimensional phase fluorescence lifetime imaging.
Lakowicz Joseph R. (10037 Fox Den Rd. Ellicott City MD 21042) Berndt Klaus W. (Baltimore MD) Hoffman Robert A. (Livermore CA) Pinsky Bertram G. (Hayward CA), Method and apparatus for performing phase fluorescence lifetime measurements in flow cytometry.
Zuckerman Ralph (226 W. Rittenhouse Square Philadelphia PA 19103), Method and apparatus for the measurement of analyte concentration levels by the steady-state determination of fluorescen.
Chao Yong-Sheng (Glaston CT) Fernandez Salvador M. (Hartford CT) Guignon Ernest F. (Canton CT), Method and appartatus for improved time-resolved fluorescence spectroscopy.
Seidel Claus,DEX ; Brand Leif,DEX ; Gunther Rolf,DEX, Method and device for determining predetermined properties of target particles of a sample medium.
Feddersen Brett A. (Urbana IL) Gratton Enrico (Urbana IL) Piston David W. (Ithaca NY), Method and means for parallel frequency acquisition in frequency domain fluorometry.
Rounbehler David P. (Bedford MA) Fine David H. (Sudbury MA) Achter Eugene K. (Lexington MA) MacDonald Stephen J. (Salem NH) Dennison Daniel B. (Kennesaw GA), Method and system for sampling and determining the presence of compounds.
Siddigi Iqbal W. (Brea CA) Sternberg James C. (Fullerton CA), Method for detecting an analyte using an electrochemical luminescent transition metal label.
Tguunanen Jukka (Helsinki FIX) Kainiemi Aimo (Espoo FIX), Method for photometrically measuring light transmitted to and through cuvettes disposed in a row.
Mabile Michel (La Celle Saint Cloud FRX) Mathis Grard (Bagnols Sur Ceze FRX) Jolu Etienne J.-P. (Bagnols Sur Ceze FRX) Pouyat Dominique (Roquemaure FRX) Dumont Christophe (Thourotte FRX), Method of measuring the luminescence emitted in a luminescent assay.
Manns Roy L. (Marshfield Hills MA) Kolb Alfred J. (Madison CT) Effertz Bernard S. (Meriden CT), Microplate farming wells with transparent bottom walls for assays using light measurements.
Manns Roy L. (Marshfield Hills MA) Kolb Alfred J. (Madison CT) Effertz Bernard S. (Meriden CT), Microplate forming wells with transparent bottom walls for assays using light measurements.
Leytes Lev J. ; Burton William G. ; Paik Yong ; Edwards Glenn R. ; Modlin Douglas N. ; El-Hage Amer, Moveable control unit for high-throughput analyzer.
Butler Michael A. ; Ricco Antonio J. ; Sinclair Michael B. ; Senturia Stephen D., Optical apparatus for forming correlation spectrometers and optical processors.
Haberland Detlef (Steinebach DEX) Langenwalter Michael (Stockdorf DEX) Panzer Klaus (Munich DEX) Rosen Hans G. (Hohenschaeftlarn DEX) Spaeter Lothar (Annweiler DEX) Spaeth Werner (Holzkirchen DEX) Se, Opto-electronic module housing.
Klein Gerald L. (Edmonds WA) Russell Gene D. (Seattle WA) Day Steven R. (Bothell WA) Liebermann Jerrold D. (Seattle WA), Photodensitometer for minimizing the refractive effects of a fluid sample.
Wechsler Mark (San Mateo CA) Barney Howard H. (Portland OR) Kaye Roger A. (Mountain View CA) Ogle David G. (Los Altos CA) Lacy Michael M. (Ben Lomond CA) Chow Calvin Y. (Portola Valley CA) Crawford K, Photometric device.
Weyrauch Bruce (Newman Lake WA) Kelln Norman (Spokane WA) Schmidt Leon (Spokane WA) Butts Charles (Spokane WA) Clark James (Spokane WA) Loughlin Kelsey (Spokane WA) Richardson Gary (Mica WA), Reagent bottle identification and reagent monitoring system for a chemical analyzer.
Weyrauch Bruce (Newman Lake WA) Kelln Norman (Spokane WA) Schmidt Leon (Spokane WA) Butts Charles (Spokane WA) Clark James (Spokane WA) Loughlin Kelsey (Spokane WA) Richardson Gary (Mica WA), Reagent bottle identification method.
Warner Brian D. (Martinez CA) Nordell Benjamin T. (Belmont CA) Richardson Bruce J. (Los Gatos CA) El-Hage Amer (Menlo Park CA), Releasable multiwell plate cover.
VanCauter Gustaaf C. (Middletown CT) Osten Donald E. (Bolingbrook IL) Tomisek John D. (Lombard IL), Scintillation counting system for in-situ measurement of radioactive samples in a multiple-well plate.
Northrup M. Allen (Berkeley CA) Mariella ; Jr. Raymond P. (Danville CA) Carrano Anthony V. (Livermore CA) Balch Joseph W. (Livermore CA), Silicon-based sleeve devices for chemical reactions.
Chow Calvin Y. H. (Portola Valley CA) Humphries Gillian M. (Los Altos CA) Kung Viola T. (Menlo Park CA) Lacy Michael M. (Ben Lomand CA) Hayter Paul (Los Altos CA), Single source multi-site photometric measurement system.
Masterson Brian K. (Placerville CA) Campbell Randolph L. (Sacramento CA) Daniel Craig M. (El Dorado Hills CA), Specimen processing and analyzing systems with associated fluid dispensing apparatus.
Monguzzi Luigi (Nova Milanese ITX) Moreni Giancarlo (Milan ITX) Simonelli Francesco (Florence ITX), Swivelling optical connector for joining optical fiber to components and sensor including such connector.
Alfano Robert R. (3777 Independence Ave. Bronx NY 10463) Liu Cheng H. (140-25 Ash Ave. Apt. #3A Flushing NY 11355) Sha Wei L. (501 W. 147th St. ; Apt. #3C New York NY 10031) Budansky Yury (736 Ramapo, Technique for determining whether a cell is malignant as opposed to non-malignant using extrinsic fluorescence spectrosc.
Gratton Enrico (Urbana IL) VandeVen Martin (Champaign IL) Barbieri Beniamino (Champaign IL), Time resolved optical array detectors and CCD cameras for frequency domain fluorometry and/or phosphorimetry.
Fernandez Salvador M. (Hartford CT) Wang Hann-Ping (Glastonbury CT) Chao Yong-Sheng (Glaston CT) Guignon Ernest F. (Canton CT), Time-resolved fluorescence immunoassay.
Zarling David A. ; Rossi Michel J.,CHX ; Peppers Norman A. ; Kane James ; Faris Gregory W. ; Dyer Mark J. ; Ng Steve Y. ; Schneider Luke V., Up-converting reporters for biological and other assays using laser excitation techniques.
Mezei ; Louis M. (Fremont CA) Albom Bradley S. (Richmond CA) Coppock ; Stan (Berkeley CA) Moehle Stephen J. (Berkeley CA) Noorda Brent S. (Pleasant Hill CA) Widunas Joseph T. (Berkeley CA) Zeitlin Ja, User controlled off-center light absorbance reading adjuster in a liquid handling and reaction system.
Herron James N. (Salt Lake City UT) Christensen Douglas A. (Salt Lake City UT) Caldwell Karin D. (Salt Lake City UT) Janatov Vera (Prague UT CSX) Huang Shao-Chie (Salt Lake City UT) Wang Hsu-Kun (Sal, Waveguide immunosensor with coating chemistry providing enhanced sensitivity.
Kochis Gary ; Embree Donald ; Meyerson Robert F. ; Lewis Calvin E., Workslate computer having modular device docking stations on horizontal and vertical side portions.
Clinton, Charles M.; Glezer, Eli N.; West, Sharon; Sigal, George; Stevens, Carl; Vock, Michael L., Continuous interleaved process for conducting an assay in a multi-well plate.
Zhang, Tuanfeng; Hurley, Neil Francis; Zhao, Weishu, Method to generate numerical pseudocores using borehole images, digital rock samples, and multi-point statistics.
Hurley, Neil Francis; Zhang, Tuanfeng; Xu, Guangping; Xu, Lili; Slim, Mirna, Method to quantify discrete pore shapes, volumes, and surface areas using confocal profilometry.
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