초록 ▼

A reagent is suitable for measuring the concentration of an analyte in a hemoglobin-containing biological fluid, such as whole blood. The reagent comprises a flavin-dependent enzyme that has specificity for the analyte, a flavin cofactor if, and only if, a flavin is not bound to the enzyme, a tetra

A reagent is suitable for measuring the concentration of an analyte in a hemoglobin-containing biological fluid, such as whole blood. The reagent comprises a flavin-dependent enzyme that has specificity for the analyte, a flavin cofactor if, and only if, a flavin is not bound to the enzyme, a tetrazolium dye precursor, an electron transfer agent, and a nitrite salt. The reagent causes dye formation that is a measure of the analyte concentration. The nitrite salt suppresses interfering dye formation caused non-enzymatically by the hemoglobin. Preferably, the reagent is used in a dry strip for measuring glucose in whole blood.

대표청구항 ▼

We claim: 1. A reagent for measuring a concentration of an analyte in a hemoglobin-containing biological fluid, comprising a) a flavin-dependent enzyme that has a flavin bound to it and that has specificity for the analyte, b) a tetrazolium dye precursor, c) an electron transfer agent, and d) a

We claim: 1. A reagent for measuring a concentration of an analyte in a hemoglobin-containing biological fluid, comprising a) a flavin-dependent enzyme that has a flavin bound to it and that has specificity for the analyte, b) a tetrazolium dye precursor, c) an electron transfer agent, and d) a nitrite salt. 2. The reagent of claim 1 in which the analyte is glucose and the enzyme is glucose oxidase. 3. The reagent of claim 1 in which the dye precursor is water-soluble. 4. The reagent of claim 1 in-which the electron transfer agent comprises phenazine methosulfate (PMS)or an analog thereof. 5. The reagent of claim 1 further comprising a divalent metal stabilizer.