IPC분류정보
국가/구분 |
United States(US) Patent
등록
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국제특허분류(IPC7판) |
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출원번호 |
US-0830863
(2004-04-22)
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발명자
/ 주소 |
- Toledo Pereyra,Luis H.
- Lopez Neblina,Fernando
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출원인 / 주소 |
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대리인 / 주소 |
Wilson Sonsini Goodrich &
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인용정보 |
피인용 횟수 :
11 인용 특허 :
17 |
초록
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A method of preserving, storing and transplanting mammalian donor organs. The method includes the cooling of refrigeration preservation, loading pre-freezer preservation, cryopreservation and washing solutions at least containing polyvinylpyrrolidone, a calcium channel blocker, a nucleoside, potassi
A method of preserving, storing and transplanting mammalian donor organs. The method includes the cooling of refrigeration preservation, loading pre-freezer preservation, cryopreservation and washing solutions at least containing polyvinylpyrrolidone, a calcium channel blocker, a nucleoside, potassium chloride, polyethylene glycol, at least one amino acid, and a steroid to a temperature of 2째 to 4째 C. and/or of 0째 to 2째 C., harvesting a donor organ, perfusing it with one or more of the solution, immersing it in one or more of the solutions and storing it at a temperature above 0째 C. or at a temperatures below 0째 C. The cryopreservation solution also contains cryopreservative agents. Preserved organs may be transplanted directly from refrigeration storage or from freezer storage by cooling the washing refrigeration preservation solutions to 2째 to 4째 C., perfusing the organ with washing solution and then preservation solution, and transplanting it.
대표청구항
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What is claimed is: 1. A method of preserving at freezer temperatures mammalian donor organs for transplantation comprising: cooling a loading, pre-freezer preservation solution at least containing polyvinylpyrrolidone-40, a calcium channel blocker, a nucleoside, potassium chloride, polyethylene gl
What is claimed is: 1. A method of preserving at freezer temperatures mammalian donor organs for transplantation comprising: cooling a loading, pre-freezer preservation solution at least containing polyvinylpyrrolidone-40, a calcium channel blocker, a nucleoside, potassium chloride, polyethylene glycol, at least one amino acid and a steroid to a temperature between 2째 C. and 4째 C.; cooling a quantity of the loading, prefreezer preservation solution to a temperature between 0째 C. and 2째 C.; cooling a cryopreservation solution at least containing polyvinylpyrrolidone-40, a calcium channel blocker, a nucleoside, potassium chloride, polyethylene glycol, at least one amino acid, a steroid, glycerol, propanediol, an alcohol and an alkyl sulfoxide to a temperature between 0째 C. and 2째 C.; inserting a needle into the isolated arterial system of the donor organ before removal from a donor; infusing the donor organ with the loading, pre-freezer preservation solution cooled to a temperature between 2째 C. and 4째 C. through the needle; harvesting the donor organ; immersing for 10 minutes or longer the donor organ in the loading, pre-freezer preservation solution cooled to a temperature between 0째 to 2째 C.; infusing the donor organ with the cryopreservation solution cooled to a temperature between 0째 to 2째 C. through the needle in a gradual manner; immersing for 10 minutes or longer the donor organ in the cryopreservation solution cooled to a temperature of 0째 C. to 2째 C.; storing the donor organ in the cryopreservation solution at a temperature below 0째 C. 2. The method of claim 1 including the further steps of storing the donor organ at a temperature below 0째 C. for 8 hours or more; and transferring the donor organ to a cryofreezer temperature of about-80째 C. 3. The method of claim 1 wherein the calcium channel blocker is verapamil. 4. The method of claim 1 wherein the nucleoside is adenosine. 5. The method of claim 1 wherein the amino acid or amino acids are selected from a group consisting of N-acetylcysteine, glycine, arginine, proline, glutamate, serine, alanine, histidine, leucine, methionine, phenylalanine and tryptophan. 6. The method of claim 1 wherein the steroid is dexamethasone. 7. The method of claim 1 wherein the loading pre-freezer preservation solution and the cryopreservation solution each have a pH between 7.0 and 7.5. 8. The method of claim 1 wherein the donor organ is a mammalian heart, liver, kidney, or pancreas. 9. A method of transplanting a donor organ that has been preserved according to claim 1, comprising cooling a washing solution at least containing (PVP-40), a calcium channel blocker, a nucleoside, potassium chloride, polyethylene glycol, at least one amino acid, a steroid, glycerol, propanediol, an alcohol and an alkyl sulfoxide to a temperature between 2째 and 4째 C. cooling a refrigeration preservation solution to a temperature between 2째 and 4째 C.; removing the donor organ from storage at a temperature below 0째 C.; perfusing the donor organ with the washing solution cooled at 2째 to 4째 C.; perfusing the donor organ with the refrigeration preservation solution cooled at 2째 and 4째 C.; and transplanting the donor organ. 10. The method of claim 9 wherein the first step of cooling of a washing solution is preceded by the transfer of a donor organ stored at cryofreezer temperatures to storage at freezer temperatures for a period of 8 hours or more. 11. The method of claim 9 wherein the calcium channel blocker is verapamil. 12. The method of claim 9 wherein the nucleoside is adenosine. 13. The method of claim 9 wherein the amino acid or amino acids are selected from a group consisting of N-acetylcysteine, glycine, arginine, proline, glutamate, serine, alanine, histidine, leucine, methionine, phenylalanine and tryptophan. 14. The method of claim 9 wherein the steroid is dexamethasone. 15. The method of claim 9 wherein the washing solution has a pH between 7.0 and 7.5.
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