IPC분류정보
국가/구분 |
United States(US) Patent
등록
|
국제특허분류(IPC7판) |
|
출원번호 |
US-0220280
(2001-03-09)
|
우선권정보 |
JP-2000-066896(2000-03-10) |
국제출원번호 |
PCT/JP01/001857
(2001-03-09)
|
§371/§102 date |
20021114
(20021114)
|
국제공개번호 |
WO01/066714
(2001-09-13)
|
발명자
/ 주소 |
- Makino,Takashi
- Aiyama,Ritsuo
- Deguchi,Yoriko
- Watanuki,Masaaki
- Nakazawa,Masako
- Mizukoshi,Harumi
- Nagaoka,Masato
- Harada,Katsuhisa
- Osada,Kuniko
|
출원인 / 주소 |
- Kabushiki Kaisha Yakult Honsha
|
대리인 / 주소 |
Oblon, Spivak, McClelland, Maier &
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인용정보 |
피인용 횟수 :
3 인용 특허 :
4 |
초록
▼
The present invention relates to an α-amylase activity inhibitor, and to food and beverages comprising the α-amylase activity inhibitor. The α-amylase activity inhibitor of the present invention exhibits remarkably excellent α-amylase inhibitory activity as compared with guava ex
The present invention relates to an α-amylase activity inhibitor, and to food and beverages comprising the α-amylase activity inhibitor. The α-amylase activity inhibitor of the present invention exhibits remarkably excellent α-amylase inhibitory activity as compared with guava extract. Accordingly, food and beverages containing the α-amylase activity inhibitor are diet food and beverages suitable for people of high blood-sugar level or hyderlipidemia.
대표청구항
▼
What is claimed is: 1. An α-amylase activity inhibitor obtained from a process which comprises: extracting guava leaves and/or guava fruit with one or more solvents selected from among water and hydrophilic solvents to obtain a resultant extract; subjecting the resultant extract to ultrafiltra
What is claimed is: 1. An α-amylase activity inhibitor obtained from a process which comprises: extracting guava leaves and/or guava fruit with one or more solvents selected from among water and hydrophilic solvents to obtain a resultant extract; subjecting the resultant extract to ultrafiltration, to thereby remove substances having a molecular weight of less than 5,000 to thereby obtain a fraction; applying the fraction to a butyl support hydrophobic chromatography column, and eluting at least one substance with an aqueous solution comprising sodium dihydrogenphosphate (0.02 mol/L) and an aqueous solution comprising sodium phosphate (0.02 mol/L) (flow rate: 1 mL/minute) under a pH gradient between the two solutions; and recovering the said at least one eluted substance, which fraction corresponds to the third single peak of an elution curve obtained when the absorbance of the substance is measured at 260 nm. 2. The α-amylase activity inhibitor according to claim 1, wherein said inhibitor comprises polyphenols which comprise the following physicochemical properties: (a) containing carbon (49.6%), hydrogen (4.6%), and nitrogen (0.6%); (b) having a molecular weight of 5,000-100,000; (c) exhibiting infrared absorption peaks at about 3,428 cm-1, 1,705 cm-1, 1,615 cm-1, and 1,220 cm-1 ; (d) exhibiting solid carbon nuclear magnetic resonance spectra corresponding to a sugar signal at 76 ppm, an aromatic signal at 115.0 ppm, phenol signals at 144 and 156 ppm, and an ester carbonyl signal at 168 ppm; and (e) exhibiting a single peak at around 10 minutes when subjected to liquid chromatography under the following conditions: column: a column packed with reversed phase synthetic polymer gel having butyl groups; flow rate: 1.5 mL/minute; column temperature: a constant temperature at about 40째 C.; detector: UV photometer (wavelength: 260 nm); mobile phase: solution A: a solution mixture comprising acetonitrile, NaH2PO4 (0.02 mol/L), and water (15:85 (v/v)) (pH=4.6), and solution B: a solution mixture comprising acetonitrile, Na 3PO4 (0.02 mol/L), and water (15:85 (v/v)) (pH=11.4); and analysis method: a step gradient analysis performed on the basis of the data listed in the following Table Analysis time (min) Solution A (%) Solution B (%) 0 ~ 4 100 0 4 ~ 8 65 35 8 ~ 12 0 100 12 ~ 20 100 0. 3. An α-amylase activity inhibitor comprising,-polyphenols having the following physicochemical properties: (a) containing carbon (49.6%), hydrogen (4.6%), and nitrogen (0.6%); (b) having a molecular weight of 5,000-100,000; (c) exhibiting infrared absorption peaks at about 3,428 cm-1, 1,705 cm-1, 1,615 cm-1, and 1,220 cm-1 ; (d) exhibiting solid carbon nuclear magnetic resonance spectra corresponding to a sugar signal at 76 ppm, an aromatic signal at 115.0 ppm, phenol signals at 144 and 156 ppm, and an ester carbonyl signal at 168 ppm; and (e) exhibiting a single peak at around 10 minutes when subjected to liquid chromatography under the following conditions: column: a column packed with reversed phase synthetic polymer gel having butyl groups; flow rate: 1.5 mL/minute; column temperature: a constant temperature of about 40째 C.; detector: UV photometer (wavelength: 260 nm); mobile phase: solution A: a solution mixture comprising acetonitrile, NaH2PO4 (0.02 mol/L), and water (15:85 (v/v)) (pH=4.6), and solution B: a solution mixture comprising acetonitrile, Na 3PO4 (0.02 mol/L), and water (15:85 (v/v)) (pH=11.4); and analysis method: a step gradient analysis performed on the basis of the data listed in the following Table Analysis time (min) Solution A (%) Solution B (%) 0 ~ 4 100 0 4 ~ 8 65 35 8 ~ 12 0 100 12 ~ 20 100 0. 4. A composition, comprising the α-amylase activity inhibitor according to any one of claims 1 through 3, which is capable of inhibiting an α-glucosidase. 5. A food or beverage comprising the α-amylase activity inhibitor as recited in any one of claims 1 through 3. 6. A method for producing a diet food or a diet beverage, which comprises: adding the α-amylase inhibitor as recited in any one of claims 1 through 3 to a food or beverage. 7. A dieting method comprising consuming the food, the beverage, or both the food and beverage as recited in claim 5. 8. A food or beverage comprising the α-amylase activity inhibitor as recited in claim 4.
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