초록 ▼

In one aspect, the invention provides new variants of the Fad3 enzyme, including amino acid substitutions, as well as nucleic acid sequences encoding such peptides. Other aspects of the invention include transgenic plants and plant parts. Vectors capable of transforming plant cells are provided, inc

In one aspect, the invention provides new variants of the Fad3 enzyme, including amino acid substitutions, as well as nucleic acid sequences encoding such peptides. Other aspects of the invention include transgenic plants and plant parts. Vectors capable of transforming plant cells are provided, including the nucleic acids of the invention, including Fad3 coding sequences. Corresponding methods are provided for obtaining the transgenic plants of the invention. Methods are provided for using the plants of the invention, including selected plants and transgenic plants, to obtain plant products. Amplification primers for identifying the Fad3 alleles of the invention are provided, together with methods of obtaining plants using the Fad3 alleles of the invention as markers.

대표청구항 ▼

What is claimed is: 1. A recombinant nucleic acid encoding a plant fatty acid desaturase, wherein the nucleic acid sequence encodes an amino acid substitution in the motif SYLRGGL (SEQ ID NO: 60) in the desaturase at the position corresponding to position 275 of Apollo Fad3 (SEQ ID NO: 1) and where

What is claimed is: 1. A recombinant nucleic acid encoding a plant fatty acid desaturase, wherein the nucleic acid sequence encodes an amino acid substitution in the motif SYLRGGL (SEQ ID NO: 60) in the desaturase at the position corresponding to position 275 of Apollo Fad3 (SEQ ID NO: 1) and wherein the substitution is the replacement of the arginine residue with an amino acid selected from the group consisting of Ile, Val, Leu, Cys, and Phe. 2. The recombinant nucleic acid of claim 1, wherein the substitution is the replacement of the arginine residue with a cysteine. 3. The recombinant nucleic acid of claim 1, wherein the nucleic acid is capable of altering the fatty acid composition of a plant. 4. An isolated vector comprising the nucleic acid of claim 1. 5. A method of modifying a plant comprising transforming the plant with a recombinant nucleic acid encoding a plant fatty acid desaturase, wherein the nucleic acid sequence encodes an amino acid substitution in the motif SYLRGGL (SEQ ID NO: 60) in the desaturase at the position corresponding to amino acid 275 of Apollo Fad3A (SEQ ID NO: 1), and wherein the substitution is the replacement of the arginine residue with an amino acid selected from the group consisting of Ile, Val, Leu, Cys, and Phe. 6. The method of claim 5, wherein the plant is selected from the group consisting of Cruciferae family: canola, rapeseed (Brassica spp.), crambe (Crambe spp.), honesty (Lunaria spp.) lesquerella (Lesquerella spp.); the Compositae family: sunflower (Helianthus spp.), safflower (Carthamus spp.), niger ( Guizotia spp.); the Palmae family: palm (Elaeis spp.), coconut (Cocos spp.), the Leguminosae family: peanut (Arachis spp.), soybean (Glycine spp.); and plants of other families such as maize (Zea spp.), cotton (Gossypium spp.), jojoba ( Simmondsia spp.), flax (Linum spp.), sesame (Sesamum spp.), castor bean (Ricinus spp.), olive (Olea spp.), poppy (Papaver spp.), spurge (Euphorbia, spp.), meadow foam ( Limnanthes spp.), mustard (Sinapis spp.) and cuphea (Cuphea spp.). 7. The method of claim 5, wherein the plant is selected from the group consisting of members of the Cruciferae family, including canola, rapeseed (Brassica spp.), crambe (Crambe spp.), honesty (Lunaria spp.) and lesquerella (Lesquerella spp.). 8. The method of claim 5, wherein the plant is a Brassica. 9. The method of claim 5, wherein the plant is canola. 10. A plant, or a part of the plant, comprising the recombinant nucleic acid of claim 1. 11. The plant or part of the plant of claim 10, wherein the plant is selected from the group consisting of Cruciferae family: canola, rapeseed (Brassica spp.), crambe (Crambe spp.), honesty ( Lunaria spp.) lesquerella (Lesquerella spp.); the Compositae family: sunflower (Helianthus spp.), safflower (Carthamus spp.), niger (Guizotia spp.); the Palmae family: palm (Elaeis spp.), coconut (Cocos spp.); the Leguminosae family: peanut (Arachis spp.), soybean (Glycine spp.); and plants of other families such as maize (Zea spp.), cotton (Gossypium spp.), jojoba (Simmondsia spp.), flax (Linum spp.), sesame ( Sesamum spp.), castor bean (Ricinus spp.), olive (Olea spp.), poppy (Papaver spp.), spurge (Euphorbia, spp.), meadow foam (Limnanthes spp.), mustard (Sinapis spp.) and cuphea (Cuphea spp.). 12. The plant or part of the plant of claim 10, wherein the plant is selected from the group consisting of members of the Cruciferae family, including canola, rapeseed (Brassica spp.), crambe ( Crambe spp.), honesty (Lunaria spp.) and lesquerella ( Lesquerella spp.). 13. The plant or part of the plant of claim 10, wherein the plant is a Brassica. 14. The plant or part of the plant of claim 10, wherein the plant is canola. 15. A method of plant selection comprising: a) obtaining a progeny plant by transformation of a parent plant with the nucleic acid of claim 1, crossing parent plant lines or self crossing of the parent plant; and, b) identifying progeny plants that comprise said nucleic acid. 16. The method of claim 15, wherein the progeny plant is selected from the group consisting of Cruciferae family: canola, rapeseed (Brassica spp.), crambe (Crambe spp.), honesty (Lunaria spp.) lesquerella (Lesquerella spp.); the Compositae family: sunflower (Helianthus spp.), safflower (Carthamus spp.), niger (Guizotia spp.); the Pa/mae family: palm (Elaeis spp.) , coconut (Cocos spp.); the Leguminosae family: peanut (Arachis spp.), soybean (Glycine spp.); and plants of other families such as maize (Zea spp.), cotton (Gossypium spp.), jojoba (Simmondsia spp.), flax (Linum spp.), sesame (Sesamum spp.), castor bean (Ricinus spp.), olive (Olea spp.), poppy (Papaver spp.), spurge (Euphorbia, spp.), meadow foam (Limnanthes spp.), mustard (Sinapis spp.) and cuphea ( Cuphea spp.). 17. The method of claim 15, wherein the progeny plant is selected from the group consisting of members of the Cruciferae family, including canola, rapeseed (Brassica spp.), crambe (Crambe spp.), honesty (Lunaria spp.) and lesquerella (Lesquerella spp.). 18. The method of claim 15, wherein the progeny plant is a Brassica. 19. The method of claim 15, wherein the progeny plant is canola. 20. A method of plant selection comprising: a) obtaining a progeny plant by (i) transformation of a parent plant with a nucleic acid that encodes an amino acid substitution in the motif SYLRGGL (SEQ ID NO: 60) in the desaturase at the position corresponding to amino acid 275 of Apollo Fad3A (SEQ ID NO: 1); and wherein the substitution is the replacement of an arginine residue with an amino acid selected from the group consisting of Ile, Val, Leu, Cys, and Phe, (ii) crossing parent plant lines or (iii) self crossing of the parent plant; and, b) identifying in the progeny plants said nucleic acid. 21. The method of claim 20, wherein the progeny plant is selected from the group consisting of Cruciferae family: canola, rapeseed (Brassica spp.), crambe (Crambe spp.), honesty (Lunaria spp.) lesquerella (Lesquerella spp.); the Compositae family: sunflower (Helianthus spp.), safflower (Carthamus spp.), niger (Guizotia spp.); the Palmae family: palm (Elaeis spp.) , coconut (Cocos spp.); the Leguminosae family: peanut (Arachis spp.), soybean (Glycine spp.), and plants of other families such as maize (Zea spp.), cotton (Gossypium spp.), jojoba (Simmondsia spp.), flax (Linum spp.), sesame (Sesamum spp.), castor bean (Ricinus spp.), olive (Olea spp.), poppy (Papaver spp.), spurge (Euphorbia, spp.), meadow foam (Limnanthes spp.), mustard (Sinapis spp.) and cuphea ( Cuphea spp.). 22. The method of claim 20, wherein the progeny plant is selected from the group consisting of members of the Cruciferae family, including canola, rapeseed (Brassica spp.), crambe (Crambe spp.), honesty (Lunaria spp.) and lesquerella (Lesquerella spp.). 23. The method of claim 20, wherein the progeny plant is a Brassica. 24. The method of claim 20, wherein the progeny plant is a canola. 25. The progeny plant or a part of the progeny plant produced by the method of claim 20. 26. The method of claim 5 wherein the substitution is the replacement of the arginine residue with an isoleucine. 27. The method of claim 5 wherein the substitution is the replacement of the arginine residue with a valine. 28. The method of claim 5 wherein the substitution is the replacement of the arginine residue with leucine. 29. The method of claim 5 wherein the substitution is the replacement of the arginine residue with cysteine. 30. The method of claim 5 wherein the substitution is the replacement of the arginine residue with phenylalanine. 31. The method of claim 20 wherein the substitution is the replacement of the arginine residue with isoleucine. 32. The method of claim 20 wherein the substitution is the replacement of the arginine residue with valine. 33. The method of claim 20 wherein the substitution is the replacement of the arginine residue with leucine. 34. The method of claim 20 wherein the substitution is the replacement of the arginine residue with cysteine. 35. The method of claim 20 wherein the substitution is the replacement of the arginine residue with phenylalanine.