This invention provides a kit comprising a substrate having a surface coated with a solid phase matrix for nucleic acid manipulation. The solid phase matrix exhibits sufficient hydrophilicity and electropositivity to tightly bind the nucleic acids in a sample. The manipulations include nucleic acid
This invention provides a kit comprising a substrate having a surface coated with a solid phase matrix for nucleic acid manipulation. The solid phase matrix exhibits sufficient hydrophilicity and electropositivity to tightly bind the nucleic acids in a sample. The manipulations include nucleic acid (double or single stranded DNA and RNA) capture from high volume and/or low concentration specimens, buffer changes, washes, and volume reductions, and enable the interface of solid phase bound nucleic acid with enzyme, hybridization or amplification strategies. The tightly bound nucleic acid may be used, for example, in repeated analyses to confirm results or test additional genes in both research and commercial applications. Further, a method for virus extraction, purification, and solid phase amplification from large volume plasma specimens is described.
대표청구항▼
We claim: 1. A kit for nucleic acid manipulation, comprising: (a) a substrate having a surface coated with a solid phase matrix, wherein said matrix is a specific binding material having one or more electropositive materials rendered hydrophilic; and (b) one or more containers comprising buffers an
We claim: 1. A kit for nucleic acid manipulation, comprising: (a) a substrate having a surface coated with a solid phase matrix, wherein said matrix is a specific binding material having one or more electropositive materials rendered hydrophilic; and (b) one or more containers comprising buffers and/or reagents necessary for manipulating said nucleic acid. 2. The kit of claim 1, wherein said substrate is in the shape of tubes, plates, membranes, capillaries, slides beads, microparticles, fibers, microchannels, and microarrays. 3. The kit of claim 1, wherein said substrate is a polymer or an oxide substrate. 4. The kit of claim 1, wherein said electropositive material comprises one or more elements selected from the group consisting of aluminum, titanium, zirconium, hafnium, scandium, yttrium, lanthanum, vanadium, tantalum, chromium, molybdenum, tungsten, boron, gallium, indium, germanium, tin, and lead. 5. The kit of claim 1, wherein said matrix is selected from the group consisting of aluminum oxide, titanium oxide (Ti2O 3), and modified zirconium dioxide (ZrO2). 6. The kit of claim 1, wherein said matrix is selected from the group consisting of alpha aluminum oxide, gamma aluminum oxide and an aluminum oxide thin-film of mixed composition. 7. The kit of claim 1, wherein said solid phase matrix is titanium oxide (Ti2O3). 8. The kit of claim 1, wherein said solid phase matrix is modified zirconium oxide (ZrO2). 9. The kit of claim 1, wherein said reagents include reagents for amplifying said nucleic acid.
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이 특허에 인용된 특허 (11)
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Rothberg, Jonathan M.; Hinz, Wolfgang; Johnson, Kim L.; Bustillo, James M.; Leamon, John H.; Schultz, Jonathan, Methods and apparatus for measuring analytes using large scale FET arrays.
Rothberg, Jonathan M.; Hinz, Wolfgang; Johnson, Kim L.; Bustillo, James; Leamon, John; Schultz, Jonathan, Methods and apparatus for measuring analytes using large scale FET arrays.
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Inman, Christina E.; Mastroianni, Alexander; Hinz, Wolfgang; Li, Shifeng; Benson, Scott C., Methods and systems for point of use removal of sacrificial material.
Inman, Christina; Mastroianni, Alexander; Hinz, Wolfgang; Li, Shifeng; Benson, Scott, Methods and systems for point of use removal of sacrificial material.
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