초록

Genes encoding microbialβ-glucuronidases and proteins and their uses are provided.

대표청구항 ▼

We claim: 1. An isolated β-glucuronidase, comprising the amino acid sequence of SEQ ID NO: 6, or a variant thereof, wherein the variant has at least 90% amino acid identity to SEQ ID NO: 6 and wherein the variant has β-glucuronidase activity. 2. An isolated fusion protein, comprising, a

We claim: 1. An isolated β-glucuronidase, comprising the amino acid sequence of SEQ ID NO: 6, or a variant thereof, wherein the variant has at least 90% amino acid identity to SEQ ID NO: 6 and wherein the variant has β-glucuronidase activity. 2. An isolated fusion protein, comprising, a β-glucuronidase that is encoded by a nucleic acid molecule comprising nucleotides 1-1689 of SEQ ID NO: 14 or by a nucleic acid molecule that hybridizes under stringent conditions to the complement of nucleotides 1-1689 of SEQ ID NO: 14 and which encodes a functional β-glucuronidase, and a peptide. 3. The fusion protein according to claim 2, wherein the peptide is hexa-His or streptavidin. 4. An isolated fusion protein, comprising a β-glucuronidase comprising the amino acid sequence of SEQ ID NO: 6 or a variant thereof, wherein the variant has at least 90% amino acid identity to SEQ ID NO: 6 and wherein the variant has β-glucuronidase activity, and a peptide. 5. The fusion protein according to claim 4, wherein the peptide is hexa-His or streptavidin.