Removal of N-terminal methionine from proteins by engineered methionine aminopeptidase
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
C12N-009/52
C12N-015/57
C12N-015/70
C12P-021/06
출원번호
US-0813549
(2004-03-29)
발명자
/ 주소
Liao,You Di
출원인 / 주소
Academia Sinica
대리인 / 주소
Fish &
인용정보
피인용 횟수 :
6인용 특허 :
13
초록▼
The present invention provides methionine aminopeptidases (MetAPs) with a broad substrate range, particularly those capable of removing the N-terminal Met from bulky or acidic penultimate residues. In preferred embodiments, these MetAPs have mutations at the 233, 206 and/or 168 positions of SEQ ID N
The present invention provides methionine aminopeptidases (MetAPs) with a broad substrate range, particularly those capable of removing the N-terminal Met from bulky or acidic penultimate residues. In preferred embodiments, these MetAPs have mutations at the 233, 206 and/or 168 positions of SEQ ID NO:1. Preferably, amino acids at these residues are substituted with glycine or threonine. Also provided are cells comprising the MetAPs, DNA encoding the MetAPs, and methods of using the MetAPs.
대표청구항▼
What is claimed is: 1. An isolated cell comprising a methionine aminopeptidase that comprises an engineered version of SEQ ID NO:1, wherein residue 206 or 233 of SEQ ID NO:1 is substituted with an amino acid selected from the group consisting of Gly, Thr, Asp, Val and Asn, and wherein the methionin
What is claimed is: 1. An isolated cell comprising a methionine aminopeptidase that comprises an engineered version of SEQ ID NO:1, wherein residue 206 or 233 of SEQ ID NO:1 is substituted with an amino acid selected from the group consisting of Gly, Thr, Asp, Val and Asn, and wherein the methionine aminopeptidase is at least 90% identical to SEQ ID NO:1 outside of residues 168, 206 and 233. 2. The cell of claim 1 wherein the methionine aminopeptidase is at least 95% identical to SEQ ID NO:1 outside of residues 168, 206 and 233. 3. The cell of claim 1 that is a bacterial cell. 4. The cell of claim 1 that is an E. coli. 5. The cell of claim 1 that is an E. coli BL21(DE3) cell. 6. The cell of claim 1 that is a eukaryotic cell. 7. An isolated nucleic acid molecule comprising a sequence that encodes a methionine aminopeptidase that comprises an engineered version of SEQ ID NO:1, wherein residue 206 or 233 of SEQ ID NO:1 is substituted with an amino acid selected from the group consisting of Gly, Thr, Asp, Val and Asn, and wherein the methionine aminopeptidase is at least 90% identical to SEQ ID NO:1 outside of residues 168, 206 and 233. 8. The nucleic acid molecule of claim 7 wherein the methionine aminopeptidase is at least 95% identical to SEQ ID NO:1 outside of residues 168, 206 and 233. 9. The nucleic acid molecule of claim 7 wherein residue 233 of the polypeptide is substituted with Gly or Thr. 10. The DNA nucleic acid molecule of claim 7 wherein residue 206 of the polypeptide is substituted with Gly, Thr or Val. 11. The DNA nucleic acid molecule of claim 7 wherein both residues 206 and 233 of the polypeptide are substituted. 12. The DNA nucleic acid molecule of claim 7 wherein the polypeptide comprises the following substitutions at residues 206 and 233: (a) residue 206 is substituted with Gly and residue 233 is substituted with Gly; (b) residue 206 is substituted with Thr and residue 233 is substituted with Gly; (c) residue 206 is substituted with Thr and residue 233 is substituted with Thr; or (d) residue 206 is substituted with Val and residue 233 is substituted with Thr. 13. The nucleic acid molecule of claim 7 wherein the polypeptide further comprises a substitution at residue 168 of SEQ ID NO:1. 14. The DNA nucleic acid molecule of claim 13 wherein residue 168 of the polypeptide is substituted with an amino acid selected from the group consisting of Gly, Ser, Thr, Val, Asp and Glu. 15. The DNA nucleic acid molecule of claim 13 wherein residue 168 of the polypeptide is substituted with Gly or Thr. 16. The nucleic acid molecule of claim 7 that is an expression vector. 17. An isolated cell comprising the nucleic acid molecule of claim 7. 18. The cell of claim 17 that is a bacterial cell. 19. The cell of claim 17 that is an E. coli. 20. The cell of claim 17 that is an E. coli BL21(DE3) cell. 21. The cell of claim 17 that is a eukaryotic cell. 22. A method of removing the N-terminal methionine from a target protein comprising (i) introducing the nucleic acid molecule of claim 7 into a cell, wherein the cell comprises a nucleic acid molecule that encodes the target protein, and (ii) permitting the expression of the nucleic acid molecule of claim 14, whereby the target protein is cleaved. 23. A method of removing the N-terminal methionine from a target protein, comprising (i) introducing into a cell the nucleic acid molecule of claim 7 and also introducing into the cell a nucleic acid molecule that encodes the target protein, and (ii) permitting the expression of the nucleic acid molecule of claim 14 and the nucleic acid molecule that encodes the target protein, whereby the target protein is cleaved. 24. The method of removing the N-terminal methionine from a target protein of claim 23, wherein the nucleic acid molecule of claim 7 further comprises a second nucleic acid sequence that encodes the target protein. 25. The method of claim 22 wherein the amino acid residue next to the N-terminal methionine in the target protein is selected from the group consisting of Gln, Asn, Leu, Ile, Met and His. 26. The method of claim 22 wherein the amino acid residue next to the N-terminal methionine in the target protein is selected from the group consisting of Phe, Tyr and Trp. 27. The method of claim 22 wherein the amino acid residue next to the N-terminal methionine in the target protein is selected from the group consisting of Asp and Glu.
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이 특허에 인용된 특허 (13)
Rosana Kapeller-Libermann ; Mark Williamson, 17867 a novel human aminopeptidase.
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