The present invention provides improved methods and compositions for producing oocysts. The oocysts produced according to the invention find use in the manufacture of vaccines. In preferred embodiments, the present invention provides methods and compositions for the production of Eimeria oocysts. V
The present invention provides improved methods and compositions for producing oocysts. The oocysts produced according to the invention find use in the manufacture of vaccines. In preferred embodiments, the present invention provides methods and compositions for the production of Eimeria oocysts. Vaccines containing Eimeria oocysts, sporocysts and/or sporozoites produced according to the present invention may be used to immunize birds against coccidiosis either in ovo or post hatch.
대표청구항▼
What is claimed is: 1. A method of collecting viable protozoan oocysts from an infected animal comprising: (a) obtaining feces comprising oocysts from an animal infected with a protozoan, wherein the animal is shedding oocysts from the protozoan in its feces, and (b) contacting feces comprising ooc
What is claimed is: 1. A method of collecting viable protozoan oocysts from an infected animal comprising: (a) obtaining feces comprising oocysts from an animal infected with a protozoan, wherein the animal is shedding oocysts from the protozoan in its feces, and (b) contacting feces comprising oocysts from the infected animal with a composition comprising a peroxygen compound and an organic acid, said composition having an acidic pH; thereby collecting viable protozoan oocysts from an infected animal. 2. The method of claim 1, wherein the protozoan comprises a species from the genus Eimeria. 3. The method of claim 2, wherein the Eimeria species is selected from the group consisting of E. maxima, E. mitis, E. tenella, E. acervulina, E. brunetti, E. necatrix, E. praecox, E. mivati, and a combination thereof. 4. The method of claim 1, wherein the animal is a bird. 5. The method of claim 4, wherein the bird is a turkey. 6. The method of claim 4, wherein the bird is a chicken. 7. The method of claim 1, wherein the pH of said composition is from about pH 1 to about pH 3. 8. The method of claim 1, wherein the concentration of said peroxygen compound is from about 0.1% to about 10% (v/v) or (w/v). 9. The method of claim 1, wherein said peroxygen compound is selected from the group consisting of hydrogen peroxide, sodium perborate, sodium percarbonate, magnesium peroxide, calcium peroxide, zinc peroxide, urea peroxide, and a combination thereof. 10. The method of claim 1, wherein said peroxygen compound is hydrogen peroxide. 11. The method of claim 1, wherein the concentration of hydrogen peroxide in the composition is from about 0.1% (v/v) to about 3% (v/v). 12. The method of claim 1, wherein the concentration of hydrogen peroxide in the composition is from about 0.25% (v/v) to about 1% (v/v). 13. The method of claim 1 further comprising an anti-foaming agent. 14. The method of claim 1, wherein the concentration of organic acid in the composition is from about 1% to about 1% (v/v) or (w/v). 15. The method of claim 1, wherein said organic acid comprises at least citric acid. 16. The method of claim 1, wherein said organic acid comprises at least propionic acid. 17. The method of claim 1, wherein said organic acid comprises citric acid and propionic acid. 18. The method of claim 1, wherein said composition is sterile. 19. A method of collecting viable protozoan oocysts from an infected animal comprising: (a) collecting feces comprising oocysts from an animal infected with a protozoan on a conveyer belt or sloped surface, wherein the animal is shedding oocysts from the protozoan in its feces, and (b) contacting feces comprising oocysts from the infected animal with a composition comprising a peroxygen compound and an organic acid, said composition having an acidic pH; thereby collecting viable protozoan oocysts from an infected animal. 20. The method of claim 19, wherein the animal feces are contacted with the composition on the conveyer belt or sloped surface. 21. The method of claim 19, further comprising transferring the animal feces to a collection vessel comprising the composition. 22. The method of claim 19, wherein the pH of said composition is from about pH 1 to about pH 3. 23. The method of claim 19, wherein the concentration of said peroxygen compound is from about 0.1% to about 10% (v/v) or (w/v). 24. The method of claim 19, wherein said peroxygen compound is hydrogen peroxide. 25. The method of claim 24, wherein the concentration of hydrogen peroxide in the composition is from about 0.1% (v/v) to about 3% (v/v). 26. The method of claim 19, wherein the concentration of organic acid in the composition is from about 1% to about 1% (v/v) or (w/v). 27. The method of claim 19, wherein said organic acid comprises at least citric acid. 28. The method of claim 19, wherein said organic acid comprises at least propionic acid. 29. The method of claim 19, wherein said composition is sterile. 30. The method of claims 19, wherein the protozoan comprises a species from the genus Eimeria. 31. The method of claim 30, wherein the Eimeria species is selected from the group consisting of E. maxima, E. mitis, E. tenella, E. acervulina, E. brunetti, E. necatrix, E. praecox, E. mivati, and a combination thereof. 32. The method of claim 19, wherein the animal is a bird. 33. The method of claim 32, wherein the bird is a turkey. 34. The method of claim 32, wherein the bird is a chicken. 35. A method of collecting viable protozoan oocysts from an infected bird comprising: collecting feces comprising oocysts from a bird infected with a protozoan in a the composition comprising a peroxygen compound and an organic acid, said composition having an acidic pH, wherein the bird is shedding oocysts from the protozoan in its feces, thereby collecting viable protozoan oocysts from an infected bird. 36. The method of claim 35, wherein the pH of said composition is from about pH 1 to about pH 3. 37. The method of claim 35, wherein the concentration of said peroxygen compound is from about 0.1% to about 10% (v/v) or (w/v). 38. The method of claim 35, wherein said peroxygen compound is hydrogen peroxide. 39. The method of claim 38, wherein the concentration of hydrogen peroxide in the composition is from about 0.1% (v/v) to about 3% (v/v). 40. The method of claim 35, wherein the concentration of organic acid in the composition is from about 1% to about 1% (v/v) or (w/v). 41. The method of claim 35, wherein said organic acid comprises at least citric acid. 42. The method of claim 35, wherein said organic acid comprises at least propionic acid. 43. The method of claim 35, wherein the bird is a turkey. 44. The method of claims 35, wherein the protozoan comprises a species from the genus Eimeria. 45. The method of claim 44, wherein the Eimeria species is selected from the group consisting of E. maxima, E. mitis, E. tenella, E. acervulina, E. brunetti, E. necatrix, E. praecox, E. mivati, and a combination thereof. 46. The method of claim 35, wherein said composition is sterile. 47. The method of claim 35, wherein the bird is a chicken.
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이 특허에 인용된 특허 (50)
Anderson David M. (Rockville MD) McCandliss Russell J. (Gaithersburg MD) Strausberg Susan L. (Silver Spring MD) Strausberg Robert L. (Silver Spring MD) Ruff Michael D. (Bowie MD) Danforth Harry D. (S, 25 KD coccidial antigen of eimeria tenella.
Murray Peter K. (Red Bank NJ) Bhogal Balbir S. (Avenel NJ) Jacobson Ethel B. (New York NY) Crane Mark S. (Westfield NJ) Schmatz Dennis M. (Cranford NJ) Galuska Stefan (North Plainfield NJ), Eimeria acervulina immunogens.
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Miller Timothy J. (Lincoln NE) Clare Robert A. (Malvern PA) Lufburrow Patricia (Sacramento CA), Continuous cell line and vaccine against avian coccidia.
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Bhogal Balbir S. (Midlothian VA) Williams Michael G. (Midlothian VA) Miller Glenn A. (Richmond VA), Live vaccine for coccidiosis utilizing coccidial sporozoites.
Phelps Patricia V. (Raleigh NC) Tyczkowski Julius K. (Cary NC) O\Connell Toni O. (Zebulon NC) Gore Ann B. (Raleigh NC), Method of introducing material into eggs during early embryonic development.
Becker Dieter J. (30 ; Hohenloher Ring D 2081 Bnningstedt DEX), Process for the purification of sewage while recapturing the fatty and albuminous matter in reusable form.
Binger Mary-Helen (Hopewell NJ) Chizzonite Richard Anthony (South Kent CT) Kramer Richard Allen (West Orange NJ) Lomedico Peter Thomas (Montclair NJ) McAndrew Stephen J. (Meriden CT) Altenburger Wern, Recombinant coccidiosis vaccines.
McDonald Vincent (Cambridge GBX) Shirley Martin W. (Buckden GBX), Vaccines for coccidiosis comprising live sporulated oocysts from strains of eimeria species.
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