Multi-analyte diagnostic system and computer implemented process for same
원문보기
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
G01N-021/29
G01N-021/25
G01N-021/41
G01B-005/28
G01F-001/00
G01F-017/00
출원번호
US-0442130
(2003-05-21)
등록번호
US-7267798
(2007-09-11)
발명자
/ 주소
Chandler,Van S.
출원인 / 주소
Luminex Corporation
대리인 / 주소
Daffer McDaniel, LLP
인용정보
피인용 횟수 :
35인용 특허 :
22
초록▼
A multi-analyte diagnostic system for use with a computer. The diagnostic system includes a flow analyzer including a co-planar light source-optical detector array, the flow analyzer being communicatable with the computer. The diagnostic system also includes a memory medium readable by the computer
A multi-analyte diagnostic system for use with a computer. The diagnostic system includes a flow analyzer including a co-planar light source-optical detector array, the flow analyzer being communicatable with the computer. The diagnostic system also includes a memory medium readable by the computer and storing computer instructions. The instructions include the following steps. A biological sample is run through the flow analyzer. The identity and quantity of at least one analyte of interest in the biological fluid is determined substantially simultaneously to the sample-running step.
대표청구항▼
What is claimed is: 1. A multi-analyte diagnostic system for use with a computer, the diagnostic system comprising: a flow analyzer including a substantially co-planar optical assembly having at least one light source and at least one optical detector, said flow analyzer being communicable with the
What is claimed is: 1. A multi-analyte diagnostic system for use with a computer, the diagnostic system comprising: a flow analyzer including a substantially co-planar optical assembly having at least one light source and at least one optical detector, said flow analyzer being communicable with the computer; and a memory medium readable by the computer and storing computer instructions, the instructions including: processing a biological sample using said flow analyzer, wherein said processing instruction includes: (a) exposing a pooled population of subsets of particles to the biological sample, the particles in each subset having (i) at least one classification parameter that distinguishes the particles of one subset from those of another subset, and (ii) a reactant specific for each of at least one analyte of interest; and (b) passing the exposed pooled population of subsets of particles through an examination zone; and determining a presence and quantity of each of said at least one analyte of interest in the biological sample substantially simultaneously to said processing step, wherein said determining instruction comprises assessing the identity and quantity of each of said at least one analyte of interest, if present, in the sample by substantially contemporaneously: (a) collecting data relating to the at least one classification parameter, including particle subset data on fluorescence emission intensities; (b) collecting data relating to a presence or absence of a complex formed between the reactant and an analyte of interest specific to the reactant, including analyte data on fluorescence emission intensities, wherein the particle subset data and the analyte data exhibit spectral overlap; (c) classifying, without relying exclusively, if at all, on differences in particle size, each particle according to its subset, wherein said classifying step includes reducing the spectral overlap sufficiently to identify said each particle according to its subset; and (d) quantifying an amount of complex associated with each subset. 2. The multi-analyte diagnostic system according to claim 1, wherein said flow analyzer further comprises: a plurality of light sources and a plurality of optical detectors, said plurality of light sources including overlapping focal regions; and a plurality of magnification lenses for magnification of light emissions or reflections detected by said at least one of the plurality of optical detectors each of the plurality of magnification lenses having a magnification factor of at least 15X. 3. The multi-analyte diagnostic system according to claim 2, wherein said plurality of light sources includes a plurality of laser diodes emitting continuous wave light. 4. The multi-analyte diagnostic system according to claim 3, wherein said plurality of laser diodes includes laser diodes emitting a plurality of wavelengths of continuous wave light. 5. The multi-analyte diagnostic system according to claim 1, wherein said flow analyzer includes a cuvette having a flat air-to-glass interface. 6. The multi-analyte diagnostic system according to claim 1, further comprising at least one of a vertically and horizontally moveable platform, wherein said flow analyzer further comprises a vertically moveable aspirator, said platform cooperating with said aspirator. 7. The multi-analyte diagnostic system according to claim 6, wherein said platform supports one of a microtiter plate and said flow analyzer. 8. The multi-analyte diagnostic system according to claim 1, wherein said flow analyzer further comprises an aspirator moveable in either one of vertical and horizontal directions. 9. The multi-analyte diagnostic system according to claim 1, further comprising a circular memory buffer communicable with said flow analyzer. 10. The multi-analyte diagnostic system according to claim 9, wherein said circular memory buffer includes a first movable pointer in operation, pointing to a storage position available for storing new data, and a second movable pointer, in operation, pointing to a storage position having unanalyzed data. 11. The multi-analyte diagnostic system according to claim 1, wherein the computer instructions further provides at least one of: a main menu; a results table; a system monitor; a dot plot display including at least one of a density dot plot and a decaying dot plot; a histogram tab; an optical amplifier control tab; a color compensation control tab; and a doublet discriminator control tab. 12. The multi-analyte diagnostic system according to claim 5, wherein said cuvette includes a substantially flat glass-to-fluid interface. 13. The multi-analyte diagnostic system according to claim 12, wherein said cuvette includes a neck region having one of an internal rectangular cross-section and an internal square cross-section. 14. The multi-analyte diagnostic system of claim 2, wherein at least one of the plurality of magnification lenses has a magnification factor substantially within a range between 20X and 40X. 15. The multi-analyte diagnostic system of claim 14, wherein two of the plurality of magnification lenses each has a magnification factor substantially equal to 25X.
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이 특허에 인용된 특허 (22)
Fleming Michael E. (Vancouver WA) Anderson Eric C. (Vancouver WA), Address generator for multi-channel circular-buffer style processing.
Wu Hai-Ping (Chapel Hill NC) Sage ; Jr. Burton H. (Raleigh NC) Adrion Robert F. (Cary NC), Apparatus and method for the detection and classification of articles using flow cytometry techniques.
Hansen W. Peter (Middleboro MA) Hoffman Robert A. (Mansfield MA), Method and apparatus for automated identification and enumeration of specified blood cell subclasses.
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Drobkov, Vladamir; Melnikov, Vladamir; Shustov, Andrey, Device and method for determining a flow velocity of a fluid or a fluid component in a pipeline.
Pensak, Jr., Stanley P.; Heden, John; Ciavarini, Steven J.; Lamoureux, John; Soares, Miguel, Devices, systems and methods for flow-compensating pump-injector synchronization.
Goddard, Gregory R; Kaduchak, Gregory; Jett, James H; Graves, Steven W, Method for non-contact particle manipulation and control of particle spacing along an axis.
Goddard, Gregory Russ; Kaduchak, Gregory; Jett, James Hubert; Graves, Steven Wayde, Method for non-contact particle manipulation and control of particle spacing along an axis.
Graves, Steven W.; Habbersett, Robert C., System and method for measuring particles in a sample stream of a flow cytometer using low-power laser source.
Graves, Steven W.; Habbersett, Robert C., System and method for measuring particles in a sample stream of a flow cytometer using low-power laser source.
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