Microfabricated crossflow devices and methods
원문보기
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
C12M-001/34
G01N-033/50
출원번호
US-0953103
(2001-09-14)
등록번호
US-7294503
(2007-11-13)
발명자
/ 주소
Quake,Stephen R.
Thorsen,Todd
출원인 / 주소
California Institute of Technology
대리인 / 주소
Townsend and Townsend and Crew LLP
인용정보
피인용 횟수 :
240인용 특허 :
83
초록▼
A microfluidic device for analyzing and/or sorting biological materials (e.g., molecules such as polynucleotides and polypeptides, including proteins and enzymes; viruses and cells) and methods for its use are provided. The device and methods of the invention are useful for sorting particles, e.g.
A microfluidic device for analyzing and/or sorting biological materials (e.g., molecules such as polynucleotides and polypeptides, including proteins and enzymes; viruses and cells) and methods for its use are provided. The device and methods of the invention are useful for sorting particles, e.g. virions. The invention is also useful for high throughput screening, e.g. combinatorial screening. The microfluidic device comprises a main channel and an inlet region in communication with the main channel at a droplet extrusion region. Droplets of solution containing the biological material are deposited into the main channel through the droplet extrusion region. A fluid different from and incompatible with the solution containing the biological material flows through the main channel so that the droplets containing the biological material do not diffuse or mix. Biological material within the droplets can be analyzed and/or sorted by detecting a predetermined characteristic of the biological sample in each droplet and sorting the droplet accordingly.
대표청구항▼
What is claimed is: 1. A microfluidic product comprising: (a) a main channel with a diameter of between about 2 and 100 microns or cross-sectional dimensions in the range of 1 to 100 microns in fluidic communication with a source of extrusion fluid passing therethrough; (b) a second microfluidic ch
What is claimed is: 1. A microfluidic product comprising: (a) a main channel with a diameter of between about 2 and 100 microns or cross-sectional dimensions in the range of 1 to 100 microns in fluidic communication with a source of extrusion fluid passing therethrough; (b) a second microfluidic channel, wherein said channel has a diameter in the range of 2 to 100 microns or cross-sectional dimensions in the range of 1 to 100 microns and is in fluidic communication with a source of sample fluid, and said sample fluid is immiscible with the extrusion fluid; and (c) said second microfluidic channel having at least one inlet region in communication with the main channel at a droplet extrusion region, the inlet region having a particle containing sample fluid immiscible with the extrusion fluid passing therethrough and constructed and arranged so that droplets of the sample fluid are sheared into the main channel at the extrusion region resulting in discrete sample fluid droplets containing particles in the extrusion fluid stream. 2. The product of claim 1 wherein the extrusion fluid is a non-polar solvent. 3. The product of claim 2 wherein the extrusion fluid is decane. 4. The product of claim 2 wherein the extrusion fluid is selected from the group consisting of tetradecane and hexadecane. 5. The product of claim 1 wherein the sample solution comprises an aqueous solution. 6. The product of claim 5 wherein the aqueous solution comprises ultrapure water, TE buffer, phosphate buffered saline or acetate. 7. The product of claim 1 wherein the particles are biological material. 8. The product of claim 7 wherein at least one of the droplets contains no more than one particle of the biological material. 9. The product of claim 8 wherein the biological material comprises viral particles and at least one of the droplets contains no more than one viral particle. 10. The product of claim 7 wherein the biological material comprises one or more cells. 11. The product of claim 7 wherein the biological material comprises one or more viral particles. 12. The product of claim 1 wherein the droplet extrusion region comprises a T-shaped junction between the inlet region and the main channel. 13. The product of claim 1 further comprising: a detection region within or coincident with at least a portion of the main channel at or downstream from the droplet extrusion region, and a detector associated with the detection region. 14. The product of claim 13 wherein the detector is an optical detector. 15. The product of claim 13 further comprising: a discrimination region downstream from the detection region, and a flow control system responsive to the detector and adapted to direct droplets through the discrimination region into a branch channel. 16. The product of claim 15, wherein the flow control comprises the application of radiation pressure. 17. The product of claim 15, wherein the flow control comprises an optical beam. 18. The product of claim 1 wherein the main channel resides in a layer of elastomeric material. 19. The product of claim 18 wherein the elastomeric layer is adjacent to a substrate layer. 20. The product of claim 1, further comprising: a first inlet region in communication with the main channel at a first droplet extrusion region; and a second inlet region in communication with the main channel at a second droplet extrusion region. 21. The product of claim 20 wherein: a first fluid containing a first particle passes through the first inlet region, so that a droplet of the fluid containing the first particle is sheared into the main channel; and a second fluid passes through the second inlet region, so that a droplet of the second fluid is sheared into the main channel. 22. The product of claim 21 wherein droplets containing the first particle mix with droplets of the second fluid. 23. The product of claim 22 wherein the first particle interacts with the second fluid upon said mixing. 24. The product of claim 23 wherein the interaction produces a detectable signal. 25. The product of claim 24, wherein the detectable signal is at least one of fluorescence and color change. 26. The product of claim 21, wherein the passage of each fluid through an inlet region is independently controlled. 27. The product of claim 26, wherein the passage of each fluid is controlled by at least one valve. 28. The product of claim 21, wherein droplets of the first fluid are sheared into the main channel ahead of droplets of the second fluid. 29. The product of claim 21, wherein the passage of each fluid is controlled by at least one valve. 30. The product of claim 1, wherein the sample fluid and the extrusion fluid have different refractive indexes. 31. The product of claim 1, wherein the refractive index of the sample fluid is lower than the refractive index of the extrusion fluid. 32. A product for sorting biological material comprising: (a) a microfabricated substrate having at least one main channel with a diameter of between about 2 and 100 microns or cross-sectional dimensions in the range of 1 to 100 microns in fluidic communication with a source of extrusion fluid passing therethrough, an inlet region which meets the main channel at a droplet extrusion region in fluidic communication with a source of sample fluid, wherein said sample fluid is immiscible with the extrusion fluid and said droplet extrusion region is constructed and arranged so that droplets of the sample fluid are sheared into the main channel, and at least two branch channels meeting at a junction downstream from the droplet extrusion region; (b) a detection region within or coincident with at least a portion of the main channel and associated with a detector; and (c) a flow control system responding to the detector and adapted to direct biological material into a branch channel. 33. The product of claim 32 wherein: an extrusion fluid passes through the main channel; and a sample fluid, which is incompatible with the first fluid and contains the biological material, passes through the inlet region, so that droplets of the second fluid and containing the biological material are sheared into the main channel. 34. The product of claim 33 wherein the flow control is adapted to direct the droplets into a branch channel. 35. The product of claim 33 wherein: the extrusion fluid is a non-polar solvent, and the sample fluid is an aqueous solution. 36. The product of claim 35 wherein the extrusion fluid is decane. 37. The product of claim 35 wherein the extrusion fluid is selected from the group consisting of tetradecane and hexadecane. 38. The product of claim 35 wherein the aqueous solution comprises ultra pure water, TE buffer, phosphate buffered saline, or acetate buffer. 39. The product of claim 33 wherein at least one droplet contains no more than one particle of the biological material. 40. The product of claim 39 wherein the biological material comprises viral, cell, cell component, organelle, nucleic acid, protein, enzyme, or bead particles and at least one droplet contains no more than one viral, cell, cell component, organelle, nucleic acid, protein, enzyme or bead particle. 41. The product of claim 33 wherein the biological material comprises one or more viral, cell, cell component, organelle, nucleic acid, protein, enzyme or bead particles. 42. The product of claim 33, wherein the sample fluid and the extrusion fluid have different refractive indexes. 43. The product of claim 33, wherein the refractive index of the sample fluid is lower than the refractive index of the extrusion fluid. 44. The product of claim 32, wherein the flow control comprises the application of radiation pressure. 45. The product of claim 32, wherein the flow control comprises an optical beam. 46. A microfluidic product comprising: (a) a main channel with a diameter of between about 2 and 100 microns or cross-sectional dimensions in the range of 1 to 100 microns in fluid communication with a source of extrusion fluid and having a stream of extrusion fluid passing therethrough, and (b) at least one inlet region in communication with the main channel at a droplet extrusion region, the inlet region in fluid communication with a source of particle containing sample fluid and having a particle containing sample fluid immiscible with the extrusion fluid passing therethrough and constructed and arranged so that droplets of the sample fluid are sheared into the main channel at the extrusion region resulting in discrete sample fluid droplets containing particles in the extrusion fluid stream, wherein the main channel resides in a layer of elastomeric material. 47. The product of claim 46 wherein the elastomeric layer is adjacent to a substrate layer. 48. A microfluidic product comprising: (a) a main channel having a stream of extrusion fluid passing therethrough, wherein said channel has a diameter in the range of 2 to 100 microns or cross-sectional dimensions in the range of 1 to 100 microns; (b) a source of extrusion fluid provided in communication with the main channel; (c) an inlet region in communication with the main channel at a droplet extrusion region, the inlet region having a particle containing sample fluid immiscible with the extrusion fluid passing therethrough and constructed and arranged so that droplets of the sample fluid are sheared into the main channel at the extrusion region resulting in discrete sample fluid droplets containing particles in the extrusion fluid stream; (d) a source of particle containing sample fluid provided in communication with the inlet region; wherein the particle containing sample fluid comprises (i) viral particles or (ii) animal cells. 49. A microfluidic product comprising: (a) a first microfluidic channel, wherein said channel, wherein said channel has a diameter in the range of 2 to 100 microns or cross-sectional dimensions in the range of 1 to 100 microns and is in fluidic communication with a source of extrusion fluid; (b) a second microfluidic channel, wherein said channel has a diameter in the range of 2 to 100 microns or cross-sectional dimensions in the range of 1 to 100 microns and is in fluidic communication with a source of sample fluid, and said sample fluid is immiscible with the extrusion fluid; (c) a junction at which the first channel is in fluidic communication with the second channel and said junction is constructed and arranged so that droplets of the sample fluid are sheared into the first microfludic channel; wherein when the extrusion fluid is flowed through the first channel at a first pressure and the sample fluid is flowed through the second channel at a second pressure lower than the first pressure, monodisperse droplets of aqueous solution are formed at the junction, said droplets having a diameter less than 60 microns. 50. A microfluidic product comprising: (a) a first input channel in communication with a source of an aqueous dispersed phase containing reagents to be dispersed; (b) a second input channel in communication with a source of a continuous phase that is immiscible with the dispersed phase; (c) a junction at which the first channel is in fluidic communication with the second channel; and, (d) an output channel in communication with the junction, wherein said junction is constructed and arranged so that droplets of the aqueous dispersed phase are sheared into the output channel, and wherein said first and second input channels and said output channel have a diameter in the range of 2 to 100 microns or cross-sectional dimensions in the range of 1 to 100 microns. 51. The microfluidic product of claim 50 wherein when the continuous phase is flowed through the first channel at a first pressure and the discontinuous phase is flowed through the second channel at a second pressure lower than the first pressure, monodisperse droplets of the dispersed phase having a diameter less than 60 microns are formed at the junction and flow through the output channel. 52. The microfluidic product of claim 50 wherein said first and second input channels and said output channel are from 50-100 microns wide and from 2-10 microns deep.
연구과제 타임라인
LOADING...
LOADING...
LOADING...
LOADING...
LOADING...
이 특허에 인용된 특허 (83)
Sanjoh Akira,JPX, Apparatus for crystal growth and crystal growth method employing the same.
Horres ; Jr. Charles R. (Del Mar CA), Electrochemical controlled dispensing assembly and method for selective and controlled delivery of a dispensing fluid.
Wise Kensall D. (Ann Arbor MI) Robertson Janet K. (Ann Arbor MI) Ji Jin (White Plains NY), Integrated microvalve structures with monolithic microflow controller.
Stabile Paul J. ; Ludington David Norman ; York Pamela Kay ; Rosen Arye ; Cherukuri Satyam Choudary ; Zanzucchi Peter John ; Heaney Paul, Massively parallel detection.
Feng Xiangdong ; Liu Jun ; Liang Liang, Method of bonding functional surface materials to substrates and applications in microtechnology and antifouling.
Benecke Wolfgang (Berlin DEX) Wagner Bernd (Berlin DEX) Hagedorn Rolf (Berlin DEX) Fuhr Gnter (Berlin DEX) Mller Torsten (Berlin DEX), Method of continuously separating mixtures of microscopic dielectric particles and apparatus for carrying through this m.
Santarsiero Bernard D. ; Stevens Raymond C. ; Shultz Peter G. ; Jaklevic Joseph M. ; Yegian Derek T. ; Cornell Earl ; Nordmeyer Robert A. ; Jin Jian ; Kolbe William F. ; Jones Arthur L. ; Uber Donald, Methods and apparatus for performing array microcrystallizations.
Faris Sadeg M. (24 Pocantico River Rd. Pleasantville NY 10570) Jain Kanti (18 Algonquian Trail Briarcliff Manor NY 10510), Microchannel plate technology.
Ekstrm Bjrn (Upsala SEX) Jacobson Gunilla (Upsala SEX) Ohman Ove (Upsala SEX) Sjdin Hakan (Upsala SEX), Microfluidic structure and process for its manufacture.
Aine Harry E. (30600 Page Mill Rd. Los Altos CA 94022) Block Barry (30610 Page Mill Rd. Los Altos CA 94022), Miniature valve and method of making same.
Bouteille Daniel (Ville d\Avray FRX) Duclos Michel (Verneuil l\Etang FRX) Marguet Hugues (Paris FRX) Nicolas Michel (Plaisir FRX) Petrimaux Eric (Evreux FRX), Pneumatic logic circuit.
Fujita Shozo (Atsugi JPX) Asano Koji (Ebina JPX) Hashitani Takafumi (Atsugi JPX), Process and apparatus for preparation of single crystal of biopolymer.
Anne R. Kopf-Sill ; Andrea W. Chow ; Peter C. Jann ; Morten J. Jensen ; Michael Spaid ; Colin B. Kennedy ; Michael J. Kennedy, Ultra high throughput microfluidic analytical systems and methods.
Ismagilov, Rustem F.; Shen, Feng; Kreutz, Jason E.; Sun, Bing; Du, Wenbin, Analysis devices, kits, and related methods for digital quantification of nucleic acids and other analytes.
Sun, Gang; Harris, Greg; May, Andy; Self, Kyle; Farrell, Kevin; Wyatt, Paul, Analysis engine and database for manipulating parameters for fluidic systems on a chip.
Quake, Stephen R.; Unger, Marc A.; Chou, Hou-Pu; Thorsen, Todd A.; Scherer, Axel, Apparatus and methods for conducting assays and high throughput screening.
Quake, Stephen R.; Unger, Marc A.; Chou, Hou-Pu; Thorsen, Todd A.; Scherer, Axel, Apparatus and methods for conducting assays and high throughput screening.
Quake, Stephen R.; Unger, Marc A.; Chou, Hou-Pu; Thorsen, Todd A.; Scherer, Axel, Apparatus and methods for conducting assays and high throughput screening.
Quake, Stephen R.; Unger, Mark A.; Chou, Hou-Pu; Thorsen, Todd A.; Scherer, Axel, Apparatus and methods for conducting assays and high throughput screening.
Ness, Kevin Dean; Burd, Samuel; Hindson, Benjamin Joseph; Belgrader, Phillip; Colston, Jr., Billy W., Cartridge with lysis chamber and droplet generator.
Ismagilov, Rustem F.; Chen, Delai; Liu, Weishan; Du, Wenbin, Chemistrode, a plug-based microfluidic device and method for stimulation and sampling with high temporal, spatial, and chemical resolution.
Colston, Jr., Billy Wayne; Hindson, Benjamin Joseph; Ness, Kevin Dean; Masquelier, Donald Arthur; Milanovich, Fred Paul; Modlin, Douglas N.; Riot, Vincent; Burd, Samuel; Makarewicz, Jr., Anthony Joseph; Belgrader, Phillip, Droplet-based assay system.
Pamula, Vamsee K.; Sista, Ramakrishna; Srinivasan, Vijay; Pollack, Michael G.; Eckhardt, Allen, Manipulation of beads in droplets and methods for manipulating droplets.
Pamula, Vamsee K.; Sista, Ramakrishna; Srinivasan, Vijay; Pollack, Michael G.; Eckhardt, Allen, Manipulation of beads in droplets and methods for manipulating droplets.
Fraden, Seth; Boukellal, Hakim; Jia, Yanwei; Selimovic, Seila; Rowat, Amy; Agresti, Jeremy; Weitz, David A., Manipulation of fluids, fluid components and reactions in microfluidic systems.
Fraden, Seth; Boukellal, Hakim; Jia, Yanwei; Selimovic, Seila; Rowat, Amy; Agresti, Jeremy; Weitz, David A., Manipulation of fluids, fluid components and reactions in microfluidic systems.
Stone, Howard A.; Anna, Shelley L.; Bontoux, Nathalie; Link, Darren R.; Weitz, David A.; Gitlin, Irina; Kumacheva, Eugenia; Garstecki, Piotr; Diluzio, Willow; Whitesides, George M., Method and apparatus for fluid dispersion.
Stone, Howard A.; Anna, Shelley L.; Bontoux, Nathalie; Link, Darren Roy; Weitz, David A.; Gitlin, Irina; Kumacheva, Eugenia; Garstecki, Piotr; Diluzio, Willow R.; Whitesides, George M., Method and apparatus for fluid dispersion.
Stone, Howard A.; Anna, Shelley L.; Bontoux, Nathalie; Link, Darren Roy; Weitz, David A.; Gitlin, Irina; Kumacheva, Eugenia; Garstecki, Piotr; Diluzio, Willow R.; Whitesides, George M., Method and apparatus for fluid dispersion.
Chiu, Daniel T.; Sun, Bingyun; Shelby, James Patrick; Edgar, John Scott; Jeffries, Gavin; Lorenz, Robert M.; Kuo, Jason S.; He, Mingyan; Allen, Peter B.; Mutch, Sarah; Kuyper, Christopher L.; Fiorini, Gina S.; Lim, David S. W., Method and device for biochemical detection and analysis of subcellular compartments from a single cell.
Quan, Emerson Chueng; Taylor, Colin Jon; Lee, Michael; Ceasar, Christopher G.; Harris, Greg; Sun, Gang, Method and system for microfluidic device and imaging thereof.
Quan, Emerson Chueng; Taylor, Colin Jon; Lee, Michael; Ceasar, Christopher; Harris, Greg; Sun, Gang, Method and system for microfluidic device and imaging thereof.
Quan, Emerson Chueng; Taylor, Colin Jon; Lee, Michael; Cesar, Christopher G.; Harris, Greg; Sun, Gang, Method and system for microfluidic device and imaging thereof.
Ismagilov, Rustem F; Tice, Joshua David; Gerdts, Cory John; Zheng, Bo, Method for conducting an autocatalytic reaction in plugs in a microfluidic system.
Griffiths, Andrew David; Abell, Chris; Hollfelder, Florian; Mastrobattista, Enrico, Method of synthesis and testing of combinatorial libraries using microcapsules.
Butler, William Frank; Chachisvilis, Mirianas; Dees, Robert; Hagen, Norbert; Marchand, Philippe; Raymond, Daniel E.; Tu, Eugene; Wang, Mark M.; Yang, Joon Mo; Yang, Rong; Zhang, Haichuan, Methods and apparatus for sorting cells using an optical switch in a microfluidic channel network.
Putnam, Martin A.; Branciforte, Jeffrey T.; Stanwood, Charles O., Methods and systems for epi-fluorescent monitoring and scanning for microfluidic assays.
Putnam, Martin A.; Branciforte, Jeffrey T.; Stanwood, Charles O., Methods and systems for manufacture of microarray assay systems, conducting microfluidic assays, and monitoring and scanning to obtain microfluidic assay results.
Fowler, Brian; Kimball, Jake; Maung, Myo Thu; May, Andrew; Norris, Michael C; Toppani, Dominique; Unger, Marc A.; Wang, Jing; West, Jason A. A., Methods for multiple single-cell capturing and processing using micro fluidics.
Fowler, Brian; Kimball, Jake; Maung, Myo Thu; May, Andrew; Norris, Michael C.; Toppani, Dominique G.; Unger, Marc A.; Wang, Jing; West, Jason A. A., Methods, systems and devices for multiple single-cell capturing and processing using microfluidics.
Fowler, Brian; Kimball, Jake; Maung, Myo Thu; May, Andrew; Norris, Michael C.; Toppani, Dominique G.; Unger, Marc A.; Wang, Jing; West, Jason A. A., Methods, systems and devices for multiple single-cell capturing and processing using microfluidics.
Fowler, Brian; Kimball, Jake; Maung, Myo Thu; May, Andrew; Norris, Michael C.; Toppani, Dominique G.; Unger, Marc A.; Wang, Jing; West, Jason A. A., Methods, systems and devices for multiple single-cell capturing and processing using microfluidics.
Putnam, Martin A.; Branciforte, Jeffrey T.; Stanwood, Charles O.; Tu, Jane M., Micro-tube particles for microfluidic assays and methods of manufacture.
Maerkl, Sebastian J.; Thorsen, Todd A.; Bao, Xiaoyan; Quake, Stephen R.; Studer, Vincent, Microfabricated structure having parallel and orthogonal flow channels controlled by row and column multiplexors.
Link, Darren R.; Boitard, Laurent; Branciforte, Jeffrey; Charles, Yves; Feke, Gilbert; Lu, John Q.; Marran, David; Tabatabai, Ahmadali; Weiner, Michael; Hinz, Wolfgang; Rothberg, Jonathan M., Microfluidic devices and methods of use in the formation and control of nanoreactors.
Link, Darren R.; Boitard, Laurent; Branciforte, Jeffrey; Charles, Yves; Feke, Gilbert; Lu, John Q.; Marran, David; Tabatabai, Ahmadali; Weiner, Michael; Hinz, Wolfgang; Rothberg, Jonathan M., Microfluidic devices and methods of use in the formation and control of nanoreactors.
Link, Darren; Boitard, Laurent; Branciforte, Jeffrey; Charles, Yves; Feke, Gilbert; Lu, John Q.; Marran, David; Tabatabai, Ahmadali; Weiner, Michael; Hinz, Wolfgang; Rothberg, Jonathan M., Microfluidic devices and methods of use in the formation and control of nanoreactors.
Cohen, David; May, Andrew; Pieprzyk, Martin; Fowler, Brian; Lee, Kim Huat; Yan, Jun; Zhou, Ming Fang; Ng, Seng Beng, Microfluidic devices with removable cover and methods of fabrication and application.
Cohen, David; May, Andrew; Pieprzyk, Martin; Fowler, Brian; Lee, Kim Huat; Yan, Jun; Zhou, Ming Fang; Ng, Seng Beng, Microfluidic devices with removable cover and methods of fabrication and application.
Ismagilov, Rustem F.; Zheng, Bo; Gerdts, Cory J., Microfluidic system with a plurality of sequential T-junctions for performing reactions in microdroplets.
Lim, Chwee Teck; Han, Jongyoon; Hou, Han Wei; Bhagat, Ali Asgar; Van Vliet, Krystyn J.; Lee, Wong Cheng, Microfluidics sorter for cell detection and isolation.
Ismagilov, Rustem F.; Shen, Feng; Kreutz, Jason E.; Du, Wenbin; Sun, Bing, Multivolume devices, kits and related methods for quantification and detection of nucleic acids and other analytes.
Ismagilov, Rustem F.; Shen, Feng; Kreutz, Jason E.; Du, Wenbin; Sun, Bing, Multivolume devices, kits and related methods for quantification and detection of nucleic acids and other analytes.
Ismagilov, Rustem F.; Shen, Feng; Kreutz, Jason E.; Du, Wenbin; Sun, Bing, Multivolume devices, kits and related methods for quantification of nucleic acids and other analytes.
Putnam, Martin A.; Leamon, John H.; Branciforte, Jeffrey T.; Stanwood, Charles O., PDMS membrane-confined nucleic acid and antibody/antigen-functionalized microlength tube capture elements, and systems employing them, and methods of their use.
Hardenbol, Paul; Patel, Pranav; Hindson, Benjamin; Wyatt, Paul William; Bjornson, Keith; Wu, Indira; Belhocine, Kamila, Processes and systems for preparation of nucleic acid sequencing libraries and libraries prepared using same.
Hindson, Benjamin Joseph; Ness, Kevin Dean; Colston, Jr., Billy Wayne; Milanovich, Fred Paul; Masquelier, Donald Arthur; Makarewicz, Jr., Anthony Joseph, System for droplet-based assays using an array of emulsions.
Hindson, Benjamin Joseph; Ness, Kevin Dean; Colston, Jr., Billy Wayne; Milanovich, Fred Paul; Masquelier, Donald Arthur; Makarewicz, Jr., Anthony Joseph, System for forming an array of emulsions.
Hiddessen, Amy L.; Masquelier, Donald A.; Ness, Kevin D.; Hindson, Benjamin J.; Makarewicz, Jr., Anthony J.; Chia, Erin R., System for forming emulsions.
Hiddessen, Amy L.; Ness, Kevin D.; Hindson, Benjamin J.; Masquelier, Donald A.; Chia, Erin R., System for generating droplets with pressure monitoring.
Hiddessen, Amy L.; Ness, Kevin D.; Hindson, Benjamin J.; Masquelier, Donald A.; Chia, Erin R., System for generating droplets with push-back to remove oil.
Hindson, Benjamin Joseph; Ness, Kevin Dean; Colston, Jr., Billy Wayne; Milanovich, Fred Paul; Masquelier, Donald Arthur; Makarewicz, Jr., Anthony Joseph, System for transporting emulsions from an array to a detector.
Unger, Marc; Manger, Ian D.; Lucero, Michael; Yi, Yong; Miyashita-Lin, Emily; Wienecke, Anja; Facer, Geoffrey, Thermal reaction device and method for using the same.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.