Method and apparatus for controlling the uniform smearing of a biological liquid over a substrate
원문보기
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
G01N-033/555
G01N-033/554
출원번호
US-0989172
(2004-11-15)
등록번호
US-7300804
(2007-11-27)
발명자
/ 주소
Sellek Prince,Jose
출원인 / 주소
Beckman Coulter, Inc.
인용정보
피인용 횟수 :
15인용 특허 :
6
초록▼
A method and apparatus for controlling the processing of a liquid sample, e.g., a blood sample, based on a measurement related to the sample's ability to coat a surface against which the sample is brought into contact while being routinely transported. Preferably, the sample is advanced through a tu
A method and apparatus for controlling the processing of a liquid sample, e.g., a blood sample, based on a measurement related to the sample's ability to coat a surface against which the sample is brought into contact while being routinely transported. Preferably, the sample is advanced through a tube, and the amount of sample residue remaining within the tube is measured and correlated to coating process subsequently carried out on the sample, e.g., a process for producing blood-smears on a microscope slide. In a preferred embodiment, the amount of sample residue in a blood-transport tube is used to control the motion profile (i.e., acceleration and velocity) of a drop-spreading member used to spread a blood drop on a microscope slide in an automated slide-making instrument.
대표청구항▼
What is claimed is: 1. A method for controlling the application of a coating of a liquid sample on a substrate based on the surface-coating characteristics of said liquid sample for said substrate, said method comprising the steps of (a) advancing a volume of liquid sample completely through an ope
What is claimed is: 1. A method for controlling the application of a coating of a liquid sample on a substrate based on the surface-coating characteristics of said liquid sample for said substrate, said method comprising the steps of (a) advancing a volume of liquid sample completely through an open-ended tubular member, said tubular member having coating retention characteristics at least similar to those of said substrate; (b) determining the amount of a residue of the liquid sample remaining on the interior walls of said tubular member after such volume of the liquid sample has been advanced completely through the tubular member; (c) determining from said amount of residue the surface-coating characteristics of the liquid sample specific for the coating-retention characteristics of said tubular member and said substrate; and (d) controlling subsequent coating of said liquid sample on the substrate based on the determined surface-coating characteristics of said liquid sample for said substrate. 2. The method as defined by claim 1 wherein the amount of said residue is optically detected. 3. The method as defined by claim 2 wherein said amount of residue is directly detected optically by irradiating said tubular member with electromagnetic radiation of a wavelength substantially absorbed by said liquid sample, said tubular member being substantially transparent to said radiation, and detecting the respective levels of radiation absorbed by said tubular member with and without a liquid residue present therein. 4. The method as defined by claim 3 wherein said tubular member is positioned within a light-integrating sphere in which said radiation can be introduced and detected. 5. The method as defined by claim 3 wherein said tubular member is cleansed with a liquid that is substantially transparent to said radiation prior to said step of detecting said respective levels of radiation absorbed by said tubular member. 6. A method for controlling the motion profile of a movably-mounted drop-spreading member used in an automated slide-making instrument to spread a drop of a biological liquid sample atop a substrate, said method comprising the steps of; (a) advancing a volume of sample liquid completely through an open-ended tubular member, such volume containing a drop of biological liquid sample to be spread by movement of the drop-spreading member atop said substrate, said tubular member having coating retention characteristics at least similar to those of said substrate; (b) determining the amount of a residue of the liquid sample remaining on the interior walls of said tubular member after such volume of the liquid sample has been advanced completely through the tubular member; (c) determining from said amount of residue the surface-coating characteristics of the liquid sample specific for the coating-retention characteristics of said tubular member and said substrate, and; (d) controlling the motion profile of the drop-spreading member to spread the drop of biological liquid sample atop said substrate based on the determined surface-coating characteristics of said biological liquid sample for said substrate. 7. The method as defined by claim 6 wherein said biological liquid sample comprises a blood sample. 8. The method as defined by claim 7 wherein the residue of said blood sample is optically detected. 9. The method as defined by claim 8 wherein said optical detection step is effected by irradiating said tubular member with electromagnetic radiation of a wavelength substantially absorbed by said blood sample, said tubular member being substantially transparent to said radiation, and detecting the respective levels of radiation absorbed by said tubular member with and without a residue of blood sample therein. 10. The method as defined by claim 9 wherein said tubular member is positioned within a light-integrating sphere in which said radiation can be introduced and detected. 11. The method as defined by claim 9 wherein said tubular member is cleansed with a liquid that is substantially transparent to said radiation prior to said step of detecting said respective levels of radiation absorbed by said tubular member. 12. Apparatus for controlling the application of a coating of a liquid sample on a substrate based on the surface-coating characteristics of said liquid sample for said substrate, said apparatus comprising: (a) a pump for advancing a volume of liquid sample completely through an open-ended tubular member, said tubular member having coating-retention characteristics at least similar to those of said substrate; (b) a detector for detecting the amount of a residue of the liquid sample remaining on the walls of the tubular member after such volume of sample has been advanced completely through the tubular member; and (c) a controller for (a) determining from said amount of said residue the surface-coating characteristics of said liquid sample vis-횪-vis the coating-retention of said tubular member and said substrate, and (b) for controlling the subsequent coating of said liquid sample on said substrate based on the detennined surface-coating characteristics of said liquid sample for said substrate [controlling the subsequent processing of the liquid sample based on the residue detected by said detector]. 13. The apparatus as defined by claim 12 wherein said detector comprises (i) a light-integrating sphere positioned to enclose an optically transparent portion of said tubular member through which said liquid sample containing a sample drop to be coated is advanced, (ii) a light-transmitter for introducing into the interior of said integrating spheie radiation of a wavelength absorbed by the sample liquid, (iii) a light-sensor for sensing the intensity of light within the integrating sphere, and (iv) a logic and control unit operatively coupled to said light-sensor for producing a control signal representing the difference of the light intensity within said light-integrating sphere before and after said sample liquid has passed completely therethrough. 14. Apparatus for controlling the movement of a drop-spreading member in an automated slide-making instrument of the type in which a drop of a biological liquid sample is spread upon a substrate to produce a sample-smear adapted to be visually analyzed, said apparatus comprising: (a) a pump for advancing a volume of sample liquid completely through an open-ended tubular member, said volume containing a drop of sample liquid to be spread by movement of said drop-spreading member, and said tubular member having coating-retention characteristics at least similar to those of said substrate; (b) a detector for detecting the amount of residue of the sample remaining said tubular member after said volume of sample has been advanced completely through said tubular member; and (c) a logic and control unit, operatively coupled to said detector, for controlling the movement of said drop-spreading member to spread said drop of sample atop a drop-supporting surface on the basis on the amount of sample residue detected. 15. The apparatus as defined by claim 14 wherein said sample liquid comprises a blood sample. 16. The apparatus as defined by claim 15 wherein the residue of said blood sample is optically detected. 17. The apparatus as defined by claim 15 wherein said detector comprises a source of electromagnetic radiation for irradiating said tubular member with electromagnetic radiation of a wavelength substantially absorbed by said blood sample, said tubular member being substantially transparent to said radiation, and a radiation sensor for detecting the respective levels of radiation absorbed by said tubular member with and without a residue of blood sample therein. 18. The apparatus as defined by claim 17 wherein said tubular member is positioned within a light-integrating sphere in which said radiation can be introduced and sensed. 19. The apparatus as defined by claim 17 wherein said tubular member is cleansed with a liquid that is substantially transparent to said radiation prior to detecting said respective levels of radiation absorbed by said tubular member. 20. The apparatus as defined by claim 17 wherein said electromagnetic radiation is blue or green optical radiation.
Sperber Cynthia J. (Fort Lauderdale FL) Gao Daniel Dashui (Miami FL) Graham Marshall D. (Nicholasville KY), Blood analysis system having blood storage, transport and automatic slide-making capabilities.
Jablonski Joseph W. (Pembroke NH) Carr Kevin F. (Sunapee NH), Highly efficient collection optical systems for providing light detectors such as photodetectors and the like with hemis.
Kram, Brian Howard; Marshall, Kevin David; Otter, Michael; Martin, Carl David; Jones, Jessica, Arcuate member for moving liquids along a microscope slide.
Parikh, Bhairavi; Brody, Michael D.; Stone, James; Halderman, Jonathan D., Method and device for identification of nucleated red blood cells from a maternal blood sample.
Parikh, Bhairavi; Brody, Michael D.; Stone, James; Halderman, Jonathan D., Method and device for identification of nucleated red blood cells from a maternal blood sample.
Otter, Michael; Kram, Brian Howard; Martin, Carl David; Jones, Jessica; Marshall, Kevin David; Tse, Christine; Harrison, Josh, Opposables and automated specimen processing systems with opposables.
Winkelman, James; Tanasijevic, Milenko; Zahniser, David, Systems and methods for determining a complete blood count and a white blood cell differential count.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.