[특허]Plate and method for high throughput screening

Plate and method for high throughput screening 원문보기

IPC분류정보
국가/구분	United States(US) Patent 등록
국제특허분류(IPC7판)	B01L-003/00
출원번호	US-0157562 (2002-05-29)
등록번호	US-7326385 (2008-02-05)
발명자 / 주소	Everett,Keith
출원인 / 주소	Biolex Therapeutics, Inc.
대리인 / 주소	Alston & Bird LLP
인용정보	피인용 횟수 : 0 인용 특허 : 29

초록 ▼

A multiple well plate and method for media exchange, including a body defining a plurality of cell wells each connected via a channel to one of a plurality of aspiration holes, is provided. The cell wells contain a porous, hydrophilic frit which is suspended on a ledge above a reservoir of fluid media and supports a tissue sample. The properties of the frit wick the fluid media upwards to supply the tissue sample with nutrients for growth and proliferation. Old media is aspirated from the wells by a liquid handling device which inserts a pipette tip into the aspiration holes. The pipette tip applies a suction pressure which draws the media out of the cell well, through the channel, into the aspiration hole and out through the pipette tip. New media is dispersed through the pipette tip and directly into the cell well.

대표청구항 ▼

That which is claimed: 1. An assembly for supporting a tissue which is to be bathed in a media that is regularly aspirated and refreshed by a top-loading pipette device, said assembly comprising: a porous frit; and a body having an upper surface, said body defining: a first hole having a first hole upper edge defined by the upper surface of the body and a first hole bottom portion defined within the body and below the upper surface of the body, said first hole being configured to receive the porous frit and the tissue and to hold the media bathing the porous frit and the tissue; a crescent-shaped ledge protruding into the first hole bottom portion and configured to support the frit thereon; and a second hole having a second hole upper edge defined by the upper surface of the body and a second hole bottom portion defined within the body and below the upper surface of the body, the second hole bottom portion being connected in fluid communication with the first hole bottom portion such that the pipette device can access the first hole through the second hole upper edge to aspirate the media through vacuum application and refresh the media by pumping in fresh media without removal of the tissue and the porous frit from the plate. 2. An assembly of claim 1, wherein the top surface of the body is configured to receive a cover plate disposed thereon. 3. An assembly of claim 2, further comprising a cover plate that is transparent to light transmission and wherein the first hole upper edge is configured to allow light transmission to the tissue contained in the first hole by having a height to diameter ratio of less than 2 to 1 so that growth of the tissue is promoted. 4. An assembly of claim 1, wherein the second hole has a diameter that is compatible with pre-existing pipette tips. 5. An assembly of claim 1, wherein the body is formed from a single piece of material. 6. An assembly for supporting a tissue which is to be bathed in a media that is regularly aspirated and refreshed by a top-loading pipette device, said assembly comprising: a porous frit; a body being formed from a single piece of material and having an upper surface, said body defining: a first hole having a first hole upper edge defined by the upper surface of the body and a first hole bottom portion defined within the body and below the upper surface of the body, said first hole being configured to receive the porous frit and the tissue and to hold the media bathing the porous frit and the tissue; and a second hole having a second hole upper edge defined by the upper surface of the body and a second hole bottom portion defined within the body and below the upper surface of the body, the second hole bottom portion being connected in fluid communication with the first hole bottom portion such that the pipette device can access the first hole through the second hole upper edge to aspirate the media through vacuum application and refresh the media by pumping in fresh media without removal of the tissue and the porous frit from the plate. 7. An assembly of claim 6, wherein the porous frit is constructed of a sintered polyethylene material. 8. An assembly of claim 7, wherein the frit is hydrophilic. 9. An assembly of claim 6, wherein the frit is about 0.25 inches thick. 10. An assembly of claim 6, wherein the frit has a pore size of 90 to 130 micrometers. 11. An assembly of claim 6, wherein the body further comprises a ledge protruding into the first hole bottom portion and configured to support the frit thereon. 12. An assembly of claim 1, wherein the porous frit is constructed of a sintered polyethylene material. 13. An assembly of claim 12, wherein the frit is hydrophilic. 14. An assembly of claim 1, wherein the frit is about 0.25 inches thick. 15. An assembly of claim 1, wherein the frit has a pore size of 90 to 130 micrometers.

이 특허에 인용된 특허 (29)

Lyman George (Cape Porpoise ME) Mathus Gregory (Concord MA) Root David (Lexington MA), Apparatus for growing tissue cultures in vitro.
상세보기
Lyman George (Cape Porpoise ME) Mathus Gregory (Concord MA) Root David (Lexington MA), Apparatus for growing tissue cultures in vitro.
상세보기
Lyman George (Cape Porpoise ME) Mathus Gregory (Concord MA) Root David (Lexington MA), Apparatus for growing tissue cultures in vitro.
상세보기
Tanny Gerald B. (Rehovot ILX), Apparatus for plant cell tissue culture.
상세보기
Leighton Stephen B. (Maplewood NJ) Brownstein Michael J. (Rockville MD), Array-type multiple cell injector.
상세보기
Stoppini Luc,CHX ; Correges Philippe,FRX, Cell culture device.
상세보기
Barker Susan (Tenafly NJ) Chu I-Hsi (West Orange NJ) Fedun Oresta N. (Wanaque NJ) Tyndorf Tadeusz A. (Manalapan NJ), Cell culture insert.
상세보기
Larsson Borg,CHX, Cell culture microchambers in a grid matrix sandwiched between a planar base and semipermeable membrane.
상세보기
Wolf Martin L. ; Wilson John R., Compartmentalized multiple well tissue culture plate.
상세보기
Tanklevsky Alexander,ILX ; Tanklevsky Michael,ILX ; Tanklevsky Alla,ILX, Container for explant culture, method of using same, and process, mold and apparatus of making same.
상세보기
Matsui Shigeaki (Hirakata JPX) Hirai Eiichi (Takarazuka JPX) Nakamura Junji (Suita JPX), Culture cell.
상세보기
Stevens Timothy A. (Madison NJ) Mussi Edward F. (Hewitt NJ) Henderson Douglas P. (Morristown NJ) Tyndorf Tadeusz A. (Manalapan Township ; Monmouth County NJ), Culture vessel.
상세보기
Pandey Ramesh C. ; Nathan Swami, Device and method for evaluating microorganisms.
상세보기
Carter Daniel C. (Decatur AL), Device and method for screening crystallization conditions in solution crystal growth.
상세보기
Jeffs David H. (Salt Lake City UT), Dispensing reagents in a specimen well.
상세보기
Okuno Noboru (2-10-1 Fujimino Hiratsuka-shi ; Kanagawa-ken JPX) Sei Masahiro (58-2 ; Shimo-Ochiai Isehara-shi ; Kanagawa-ken JPX) Abe Hiroshi (4-34-5 ; Soshi-gaya Setagaya-ku ; Tokyo JPX), Liquid culture apparatus.
상세보기
Miyake Shinichi (Osaka JPX) Miyasaka Shinji (Osaka JPX), Method and apparatus for incubating cells.
상세보기
Henderson Douglas P., Multi-well culture dish assembly.
상세보기
Mathus Gregory, Multi-well plate.
상세보기
Grass George M., Packing device for transporting confluent cell monolayers.
상세보기
Young Roy E. (Six Mile SC) Adelberg Jeffrey W. (Clemson SC), Plant propagation system.
상세보기
Young Roy E. (Six Mile SC) Adelberg Jeffrey W. (Clemson SC), Plant propagation system and method.
상세보기
Yang Ning-Sun (Verona WI) Paau Alan (Middleton WI), Plant tissue culture device.
상세보기
Churchouse Stephen J. (Banbury GB2) Scamans Elizabeth M. (Banbury GB2), Porous inorganic membrane support and method.
상세보기
Liner ; John, Slips for specimen growth and microscopic examination.
상세보기
Pacheco Manuel M. (Billerica MA) Pacheco John F. (Billerica MA), Supported microporous membrane.
상세보기
Root David (Lexington MA), System for growing and manipulating tissue cultures using 96-well format equipment.
상세보기
Clark Phillip (Stoneham MA), Test plate apparatus.
상세보기
Pitt Aldo M. (18 Partridge La. Sudbury MA 01776) Steuck Michael J. (89 Central St. N. Reading MA 01864), Transparent porous membrane having hydrophilic surface and process.
상세보기

내보내기 메뉴

내보내기 구분

파일저장
인쇄
메일전송

구성항목

기본정보
상세정보

관리번호, 국가코드, 자료구분, 상태, 출원번호, 출원일자, 공개번호, 공개일자, 등록번호, 등록일자, 발명명칭(한글), 발명명칭(영문), 출원인(한글), 출원인(영문), 출원인코드, 대표IPC

저장형식

Text(ASCII format)
Excel format
PIAS분석(.xls)

메일정보

받는사람 (필수): @
보내는사람 (선택): @
제목
내용: KISTI 검색결과 이메일 서비스

안내

총 건의 자료가 검색되었습니다.

다운받으실 자료의 인덱스를 입력하세요. (1-10,000)

검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다.

데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요)

다운로드 파일은 UTF-8 형태로 저장됩니다.
파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오.

Text(ASCII format)
Excel format

AI-Helper ※ AI-Helper는 을 사용합니다.

AI-Helper

안녕하세요, AI-Helper입니다. 좌측 "선택된 텍스트"에서 텍스트를 선택하여 요약, 번역, 용어설명을 실행하세요.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.

IPC	Description
A	생활필수품
A62	인명구조; 소방(사다리 E06C)
A62B	인명구조용의 기구, 장치 또는 방법(특히 의료용에 사용되는 밸브 A61M 39/00; 특히 물에서 쓰이는 인명구조 장치 또는 방법 B63C 9/00; 잠수장비 B63C 11/00; 특히 항공기에 쓰는 것, 예. 낙하산, 투출좌석 B64D; 특히 광산에서 쓰이는 구조장치 E21F 11/00)
A62B-1/08	.. 윈치 또는 풀리에 제동기구가 있는 것

연합인증

Plate and method for high throughput screening 원문보기

초록 ▼

대표청구항 ▼

연구과제 타임라인

이 특허에 인용된 특허 (29)

관련 콘텐츠

특허 원문 보기

IPC 상위 출원인

AI-Helper ※ AI-Helper는 오픈소스 모델을 사용합니다.

선택된 텍스트

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 국가코드, 자료구분, 상태, 출원번호, 출원일자, 공개번호, 공개일자, 등록번호, 등록일자, 발명명칭(한글), 발명명칭(영문), 출원인(한글), 출원인(영문), 출원인코드, 대표IPC 관리번호, 국가코드, 자료구분, 상태, 출원번호, 출원일자, 공개번호, 공개일자, 공고번호, 공고일자, 등록번호, 등록일자, 발명명칭(한글), 발명명칭(영문), 출원인(한글), 출원인(영문), 출원인코드, 대표출원인, 출원인국적, 출원인주소, 발명자, 발명자E, 발명자코드, 발명자주소, 발명자 우편번호, 발명자국적, 대표IPC, IPC코드, 요약, 미국특허분류, 대리인주소, 대리인코드, 대리인(한글), 대리인(영문), 국제공개일자, 국제공개번호, 국제출원일자, 국제출원번호, 우선권, 우선권주장일, 우선권국가, 우선권출원번호, 원출원일자, 원출원번호, 지정국, Citing Patents, Cited Patents
저장형식	Text(ASCII format) Excel format PIAS분석(.xls)
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증

Plate and method for high throughput screening 원문보기

초록 ▼

대표청구항 ▼

연구과제 타임라인

전체(0) 논문(0) 특허(0) 보고서(0)

전체(0) 논문(0) 특허(0) 보고서(0)

이 특허에 인용된 특허 (29)

관련 콘텐츠

특허 원문 보기

IPC 상위 출원인

AI-Helper ※ AI-Helper는 오픈소스 모델을 사용합니다.

선택된 텍스트