Compilations of nucleic acids and arrays and methods of using them
원문보기
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
C12Q-001/68
C12N-015/00
C12N-015/11
출원번호
US-0273399
(2002-10-15)
등록번호
US-7335470
(2008-02-26)
발명자
/ 주소
Mohammed,Mansoor
Kang,Jason
Shah,Shishir
Cai,Wei Wen
출원인 / 주소
PerkinElmer, LAS, Inc.
대리인 / 주소
Lowrie, Lando & Anastasi, LLP
인용정보
피인용 횟수 :
3인용 특허 :
130
초록▼
In one aspect the invention provides compilations of nucleic acids, articles of manufacture, e.g., arrays, and methods for the detection of chromosomal abnormalities, such as a chromosomal aneuploidies, deletions, amplifications, and the like, and the diagnosis or prognosis of syndromes associated w
In one aspect the invention provides compilations of nucleic acids, articles of manufacture, e.g., arrays, and methods for the detection of chromosomal abnormalities, such as a chromosomal aneuploidies, deletions, amplifications, and the like, and the diagnosis or prognosis of syndromes associated with a contiguous gene abnormality. Kits are also provided.
대표청구항▼
What is claimed is: 1. An array comprising: a plurality of nucleic acid segments, wherein each nucleic acid segment is immobilized to a discrete and known spot on a substrate surface to form an array of nucleic acids, and each spot comprises a segment of genomic nucleic acid selected to detect a ge
What is claimed is: 1. An array comprising: a plurality of nucleic acid segments, wherein each nucleic acid segment is immobilized to a discrete and known spot on a substrate surface to form an array of nucleic acids, and each spot comprises a segment of genomic nucleic acid selected to detect a genetic syndrome, wherein the array-immobilized genomic nucleic acid segments are covalently bound to the substrate surface through a compound having the general formula: R1--X--R2, and wherein R1 is a cyclic ether, an aldehyde, or a chloromethylphenyl moiety; X is a moiety chemically suitable for linking the R1 moiety to the R2 moiety, and the R2 moiety has the general formula wherein R3, R4 and R5 comprise identical or different alkoxy groups or chloro groups. 2. The away of claim 1, further comprising at least one spot comprising a nucleic acid segment acting as a positive control. 3. The array of claim 1, further comprising at least one spot comprising a nucleic acid segment acting as a negative control. 4. The array of claim 1, wherein the array-immobilized genomic nucleic acid segments in a first spot are non-overlapping in sequence compared to the array-immobilized genomic nucleic acid segments in a second spot. 5. The array of claim 4, wherein the array-immobilized genomic nucleic acid segments in the first spot are non-overlapping in sequence compared to the array-immobilized genomic nucleic acid segments in all other genomic nucleic acid-comprising spots on the array. 6. The array of claim 1, wherein at least one genomic nucleic acid segment is spotted in duplicate or triplicate on the array. 7. The array of claim 1, wherein the duplicate spot or triplicate spot has a different amount of nucleic acid segments immobilized. 8. The array of claim 6, wherein all the genomic nucleic acid segments are spotted in duplicate or triplicate on the array. 9. The array of claim 1, wherein about 95% of the array-immobilized genomic nucleic acid segments comprise a label. 10. The array of claim 1, wherein about 98% of the array-immobilized genomic nucleic acid segments comprise a label. 11. The array of claim 1, wherein 100% of the array-immobilized genomic nucleic acid segments comprise a label. 12. The array of claim 1, wherein at least one nucleic acid segment is cloned in a construct comprising an artificial chromosome. 13. The array of claim 12, wherein the artificial chromosome comprises a bacterial artificial chromosome (BAC). 14. The array of claim 12, wherein the artificial chromosome is selected from the group consisting of a human artificial chromosome (HAC) a yeast artificial chromosome (YAC), a transformation-competent artificial chromosome (TAC) and a bacteriophage P1-derived artificial chromosome (PAC). 15. The array of claim 1, wherein at least one nucleic acid segment is cloned in a construct comprising a vector selected from the group consisting of a cosmid vector, a plasmid vector and a viral vector. 16. The array of claim 1, wherein at least one of the nucleic acid segments is between about 50 kilobases to about 500 kilobases in length. 17. The array of claim 16, wherein the at least one nucleic acid segment is between about 100 kilobases to about 400 kilobases in length. 18. The array of claim 17, wherein the at least one nucleic acid segment is about 300 kilobases in length. 19. A compilation of nucleic acids for diagnosis of a genetic syndrome, the compilation comprising a plurality of nucleic acid segments, wherein each nucleic acid segment comprises a segment of genomic nucleic acid selected to detect a genetic syndrome, wherein the nucleic acid segments further comprise a compound having the general formula description="In-line Formulae" end="lead"R1--X--Si(OR2)m(Cl)n(R) k,description="In-line Formulae" end="tail" wherein m+k is the integer 3, and n can be 0 if m is greater than 0, or n+k is the integer 3 and m can be 0 if n is greater than 0; X is an inert linker; R1 comprises a group reactive toward the biological molecule; R is an alkyl group; and R2 is an alkyl group. 20. The compilation of nucleic acids of claim 19, wherein the nucleic acid segments further comprise a cloning vehicle. 21. The compilation of nucleic acids of claim 19, wherein the nucleic acid segments are immobilized onto a surface. 22. The compilation of nucleic acids of claim 21, wherein the nucleic acid segments are immobilized on a surface as an array. 23. The compilation of nucleic acids of claim 19, wherein about 95% of the nucleic acid segments comprise a label. 24. The compilation of nucleic acids of claim 19, wherein about 98% of the nucleic acid segments comprise a label. 25. The compilation of nucleic acids of claim 19, wherein 100% of the nucleic acid segments comprise a label. 26. The compilation of nucleic acids of claim 19, wherein at least one nucleic acid segment is cloned in a construct comprising an artificial chromosome. 27. The compilation of nucleic acids of claim 26, wherein the artificial chromosome comprises a bacterial artificial chromosome (BAC). 28. The compilation of nucleic acids of claim 26, wherein the artificial chromosome is selected from the group consisting of a human artificial chromosome (HAC), a yeast artificial chromosome (YAC), a transformation-competent artificial chromosome (TAC) and a bacteriophage P1-derived artificial chromosome (PAC). 29. The compilation of nucleic acids of claim 19, wherein at least one nucleic acid segment is cloned in a construct comprising a vector selected from the group consisting of a cosmid vector, a plasmid vector and a viral vector. 30. The compilation of nucleic acids of claim 19, wherein the nucleic acid segments are between about 50 kilobases to about 500 kilobases in length. 31. The compilation of nucleic acids of claim 30, wherein the nucleic acid segments are between about 100 kilobases to about 400 kilobases in length. 32. The compilation of nucleic acids of claim 31, wherein the cloned nucleic acid segments are between about 150 kilobases and about 300 kilobases in length. 33. An array comprising: a plurality of nucleic acid segments, wherein each nucleic acid segment is immobilized to a discrete and known spot on a substrate surface to form an array of nucleic acids, and each spot comprises a segment of genomic nucleic acid selected to detect a genetic syndrome, wherein the array-immobilized genomic nucleic acid segments are covalently bound to the substrate surface through a compound having the general formula: R1--X--R2, wherein R1 is an amino group, R2 is an alkoxysilane group or a chlorohalide group; and X is a moiety chemically suitable for linking the R1 group and the R2 group. 34. The array of claim 31, further comprising at least one spot comprising a nucleic acid segment acting as a positive control. 35. The array of claim 31, further comprising at least one spot comprising a nucleic acid segment acting as a negative control. 36. The array of claim 31, wherein the array-immobilized genomic nucleic acid segments in a first spot are non-overlapping in sequence compared to the array-immobilized genomic nucleic acid segments in a second spot. 37. The array of claim 36, wherein the array-immobilized genomic nucleic acid segments in the first spot are non-overlapping in sequence compared to the array-immobilized genomic nucleic acid segments in all other genomic nucleic acid-comprising spots on the array. 38. The array of claim 31, wherein at least one genomic nucleic acid segment is spotted in duplicate or triplicate on the array. 39. The array of claim 38, wherein the duplicate spot or triplicate spot has a different amount of nucleic acid segments immobilized. 40. The array of claim 38, wherein all the genomic nucleic acid segments are spotted in duplicate or triplicate on the array. 41. The array of claim 31, wherein about 95% of the array-immobilized genomic nucleic acid segments comprise a label. 42. The array of claim 31, wherein about 98% of the array-immobilized genomic nucleic acid segments comprise a label. 43. The array of claim 31, wherein 100% of the array-immobilized genomic nucleic acid segments comprise a label. 44. The array of claim 31, wherein at least one nucleic acid segment is cloned in a construct comprising an artificial chromosome. 45. The array of claim 44, wherein the artificial chromosome comprises a bacterial artificial chromosome (BAC). 46. The array of claim 44, wherein the artificial chromosome is selected from the group consisting of a human artificial chromosome (HAC) a yeast artificial chromosome (YAC), a transformation-competent artificial chromosome (TAC) and a bacteriophage P1-derived artificial chromosome (PAC). 47. The array of claim 31, wherein at least one nucleic acid segment is cloned in a construct comprising a vector selected from the group consisting of a cosmid vector, a plasmid vector and a viral vector. 48. The array of claim 31, wherein at least one of the nucleic acid segments is between about 50 kilobases to about 500 kilobases in length. 49. The array of claim 48, wherein the at least one nucleic acid segment is between about 100 kilobases to about 400 kilobases in length. 50. The array of claim 49, wherein the at least one nucleic acid segment is about 300 kilobases in length. 51. A method for selecting a genomic nucleic acid segment for use as a hybridization target in a comparative genomic hybridization (CGH) reaction for the detection of a chromosomal aneuploidy comprising (a) selecting a chromosomal segment that hybridizes to a single locus comprising a segment of the chromosome comprising the aneuploidy to be detected; (b) selecting a chromosomal segment having at least 15% to 25% unique sequence such that at least 75% to 85% of the sequence within the chromosomal segment is repetitive, except for chromosomal segments from the X chromosome or Y chromosome, which can have up to 90% to 95% repetitive sequences; and (c) selecting a segment selected in both step (a) and step (b), thereby selecting a genomic nucleic acid segment for use as a hybridization target in a comparative genomic hybridization (CGH) reaction for the detection of a chromosomal aneuploidy. 52. The method of claim 51 comprising selecting a chromosomal segment having at least 15% unique sequence. 53. A compilation of nucleic acids for diagnosis of a genetic syndrome, the compilation comprising a plurality of nucleic acid segments, wherein each nucleic acid segment comprises a segment of genomic nucleic acid selected to detect a genetic syndrome, wherein the nucleic acid segments further comprise a compound having the general formula: R1--X--R2, wherein R1 is a cyclic ether, an aldehyde, or a chloromethylphenyl moiety; X is a moiety chemically suitable for linking the R1 moiety to the R2 moiety, and the R2 moiety has the general formula wherein R3, R4 and R5 comprise identical or different alkoxy groups or chloro groups. 54. The compilation of nucleic acids of claim 53, wherein the nucleic acid segments further comprise a cloning vehicle. 55. The compilation of nucleic acids of claim 53, wherein the nucleic acid segments are immobilized onto a surface. 56. The compilation of nucleic acids of claim 55, wherein the nucleic acid segments are immobilized on a surface as an array. 57. The compilation of nucleic acids of claim 53, wherein about 95% of the nucleic acid segments comprise a label. 58. The compilation of nucleic acids of claim 53, wherein about 98% of the nucleic acid segments comprise a label. 59. The compilation of nucleic acids of claim 53, wherein 100% of the nucleic acid segments comprise a label. 60. The compilation of nucleic acids of claim 53, wherein at least one nucleic acid segment is cloned in a construct comprising an artificial chromosome. 61. The compilation of nucleic acids of claim 60, wherein the artificial chromosome comprises a bacterial artificial chromosome (BAC). 62. The compilation of nucleic acids of claim 60, wherein the artificial chromosome is selected from the group consisting of a human artificial chromosome (HAC), a yeast artificial chromosome (YAC), a transformation-competent artificial chromosome (TAC) and a bacteriophage P1-derived artificial chromosome (PAC). 63. The compilation of nucleic acids of claim 53, wherein at least one nucleic acid segment is cloned in a construct comprising a vector selected from the group consisting of a cosmid vector, a plasmid vector and a viral vector. 64. The compilation of nucleic acids of claim 53, wherein the nucleic acid segments are between about 50 kilobases to about 500 kilobases in length. 65. The compilation of nucleic acids of claim 64, wherein the nucleic acid segments are between about 100 kilobases to about 400 kilobases in length. 66. The compilation of nucleic acids of claim 65, wherein the cloned nucleic acid segments are between about 150 kilobases and about 300 kilobases in length. 67. A compilation of nucleic acids for diagnosis of a genetic syndrome, the compilation comprising a plurality of nucleic acid segments, wherein each nucleic acid segment comprises a segment of genomic nucleic acid selected to detect a genetic syndrome, wherein the nucleic acid segments further comprise a compound having the general formula: R1--X--R2, wherein R1 is an amino group, R2 is an alkoxysilane group or a chlorohalide group; and X is a moiety chemically suitable for linking the R1 group and the R2 group. 68. The compilation of nucleic acids of claim 67, wherein the nucleic acid segments further comprise a cloning vehicle. 69. The compilation of nucleic acids of claim 67, wherein the nucleic acid segments are immobilized onto a surface. 70. The compilation of nucleic acids of claim 69, wherein the nucleic acid segments are immobilized on a surface as an array. 71. The compilation of nucleic acids of claim 67, wherein about 95% of the nucleic acid segments comprise a label. 72. The compilation of nucleic acids of claim 67, wherein about 98% of the nucleic acid segments comprise a label. 73. The compilation of nucleic acids of claim 67, wherein 100% of the nucleic acid segments comprise a label. 74. The compilation of nucleic acids of claim 67, wherein at least one nucleic acid segment is cloned in a construct comprising an artificial chromosome. 75. The compilation of nucleic acids of claim 74, wherein the artificial chromosome comprises a bacterial artificial chromosome (BAC). 76. The compilation of nucleic acids of claim 74, wherein the artificial chromosome is selected from the group consisting of a human artificial chromosome (HAC), a yeast artificial chromosome (YAC), a transformation-competent artificial chromosome (TAC) and a bacteriophage P1-derived artificial chromosome (PAC). 77. The compilation of nucleic acids of claim 67, wherein at least one nucleic acid segment is cloned in a construct comprising a vector selected from the group consisting of a cosmid vector, a plasmid vector and a viral vector. 78. The compilation of nucleic acids of claim 67, wherein the nucleic acid segments are between about 50 kilobases to about 500 kilobases in length. 79. The compilation of nucleic acids of claim 78, wherein the nucleic acid segments are between about 100 kilobases to about 400 kilobases in length. 80. The compilation of nucleic acids of claim 79, wherein the cloned nucleic acid segments are between about 150 kilobases and about 300 kilobases in length. 81. An array comprising: a plurality of nucleic acid segments, wherein each nucleic acid segment is immobilized to a discrete and known spot on a substrate surface to form an array of nucleic acids, and each spot comprises a segment of genomic nucleic acid selected to detect a genetic syndrome, wherein the array-immobilized genomic nucleic acid segments are covalently bound to the substrate surface through a compound having the general formula description="In-line Formulae" end="lead"R1--X--Si(OR2)m(Cl)n(R) k,description="In-line Formulae" end="tail" wherein m+k is the integer 3, and n can be 0 if m is greater than 0, or n+k is the integer 3 and m can be 0 if n is greater than 0; X is an inert linker; R1 comprises a group reactive toward a biological molecule; R is an alkyl group; and, R2 is an alkyl group. 82. The array of claim 81, further comprising at least one spot comprising a nucleic acid segment acting as a positive control. 83. The array of claim 81, further comprising at least one spot comprising a nucleic acid segment acting as a negative control. 84. The array of claim 81, wherein the array-immobilized genomic nucleic acid segments in a first spot are non-overlapping in sequence compared to the array-immobilized genomic nucleic acid segments in a second spot. 85. The array of claim 84, wherein the array-immobilized genomic nucleic acid segments in the first spot are non-overlapping in sequence compared to the array-immobilized genomic nucleic acid segments in all other genomic nucleic acid-comprising spots on the array. 86. The array of claim 81, wherein at least one genomic nucleic acid segment is spotted in duplicate or triplicate on the array. 87. The array of claim 86, wherein the duplicate spot or triplicate spot has a different amount of nucleic acid segments immobilized. 88. The array of claim 86, wherein all the genomic nucleic acid segments are spotted in duplicate or triplicate on the array. 89. The array of claim 81, wherein about 95% of the array-immobilized genomic nucleic acid segments comprise a label. 90. The array of claim 81, wherein about 98% of the array-immobilized genomic nucleic acid segments comprise a label. 91. The array of claim 81, wherein 100% of the array-immobilized genomic nucleic acid segments comprise a label. 92. The array of claim 81, wherein at least one nucleic acid segment is cloned in a construct comprising an artificial chromosome. 93. The array of claim 92, wherein the artificial chromosome comprises a bacterial artificial chromosome (BAC). 94. The array of claim 92, wherein the artificial chromosome is selected from the group consisting of a human artificial chromosome (HAC) a yeast artificial chromosome (YAC), a transformation-competent artificial chromosome (TAC) and a bacteriophage P1-derived artificial chromosome (PAC). 95. The array of claim 81, wherein at least one nucleic acid segment is cloned in a construct comprising a vector selected from the group consisting of a cosmid vector, a plasmid vector and a viral vector. 96. The array of claim 81, wherein at least one of the nucleic acid segments is between about 50 kilobases to about 500 kilobases in length. 97. The array of claim 96, wherein the at least one nucleic acid segment is between about 100 kilobases to about 400 kilobases in length. 98. The array of claim 97, wherein the at least one nucleic acid segment is about 300 kilobases in length.
연구과제 타임라인
LOADING...
LOADING...
LOADING...
LOADING...
LOADING...
이 특허에 인용된 특허 (130)
Menchen Steven M. (Fremont CA) Lee Linda G. (Palo Alto CA) Connell Charles R. (Redwood City CA) Hershey N. Davis (San Carlos CA) Chakerian Vergine (San Mateo CA) Woo Sam (Redwood City CA) Fung Steven, 4,7-dichlorofluorescein dyes as molecular probes.
Garini Yuval,ILX ; Cabib Dario,ILX ; Buckwald Robert A.,ILX ; Soenksen Dirk G. ; Katzir Nir,ILX ; Wine David,ILX ; Lavi Moshe,ILX, Color display of chromosomes or portions of chromosomes.
Pinkel Daniel (Walnut Creek CA) Gray Joe W. (San Francisco CA) Kallioniemi Anne (Tampere FIX) Kallioniemi Olli-Pekka (Tampere FIX) Waldman Frederic (San Francisco CA), Comparative genomic hybridization (CGH).
Nadeau James G. ; Pitner J. Bruce ; Schram James L. ; Linn C. Preston ; Vonk Glenn P. ; Walker G. Terrance, Detection of nucleic acids by fluorescence quenching.
Giese Roger W. (Quincy MA) Ehrat Markus (Suhr MA CHX) Cecchini Douglas J. (Somerville MA), Enzyme activity amplification method for increasing assay sensitivity.
Giese Roger W. (Quincy MA) Ehrat Markus (Suhr CHX) Cecchini Douglas J. (Somerville MA), Enzyme amplification by using free enzyme to release enzyme from an immobilized enzyme material.
Lockhart David J. ; Brown Eugene L. ; Wong Gordon G. ; Chee Mark S. ; Gingeras Thomas R., Expression monitoring by hybridization to high density oligonucleotide arrays.
Cronin Maureen T. ; Miyada Charles Garrett ; Trulson Mark ; Gingeras Thomas R. ; McGall Glenn ; Robinson Claire ; Oval Michelle, Hybridization assays on oligonucleotide arrays.
Carrico Robert J. (Elkhart IN) Hatch Robert P. (Elkhart IN) Patterson William L. (Elkhart IN), Immobilization of nucleic acids on solvolyzed nylon supports.
Pirrung Michael C. (Durham NC) Read J. Leighton (Palo Alto CA) Fodor Stephen P. A. (Palo Alto CA) Stryer Lubert (Stanford CA), Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof.
Pham Andrew A. ; Coassin Peter J. ; Harootunian Alec Tate ; Mendlein John D. ; Tsien Roger Y., Low background multi-well plates and platforms for spectroscopic measurements.
Sargent Jeannine P. (22270 Kendle St. Cupertino CA 95014) Marks Jeff (4730 Cielo Vista Way San Jose CA 95129), Method and apparatus for determining the sequence of polynucleotides.
Trulson Mark (Santa Clara CA) Stern David (Mountain View CA) Fiekowsky Peter (Los Altos CA) Rava Richard (Palo Alto CA) Walton Ian (Menlo Park CA) Fodor Stephen P. A. (Palo Alto CA), Method and apparatus for imaging a sample on a device.
Cole Stewart,FRX ; Buchrieser-Brosch Roland,FRX ; Gordon Stephen,FRX ; Billault Alain,FRX, Method for isolating a polynucleotide of interest from the genome of a mycobacterium using a BAC-based DNA library application to the detection of mycobacteria.
Waggoner Alan S. (Pittsburgh PA) Ernst Lauren A. (Pittsburgh PA) Mujumdar Ratnakar B. (Glenshaw PA), Method for labeling and detecting materials employing arylsulfonate cyanine dyes.
Garini Yuval,ILX ; Cabib Dario,ILX ; Buckwald Robert A.,ILX ; Ried Thomas ; Soenksen Dirk G., Method for simultaneous detection of multiple fluorophores for in situ hybridization and multicolor chromosome painting and banding.
Cabib Dario (Timrat ILX) Friedman Zvi (Kiryat Bialik ILX) Lipson Stephen G. (Kaufman ILX) Buckwald Robert A. (Ramat Ishay ILX), Method for simultaneously measuring the spectral intensity as a function of wavelength of all the pixels of a two dimens.
Bahl Chander (Flemington NJ) Lang Rhonda (Lawrenceville NJ) Mendoza Leopoldo G. (Madison WI), Method of immobilizing nucleic acid on a solid surface for use in nucleic acid hybridization assays.
Mahendran Mailvaganam,CAX ; Goodboy Kenneth Paul ; Bai Jinhua,CAX, Method of making a dope comprising hydrophilized PVDF and .alpha.-alumina, and a membrane made therefrom.
Ried Thomas ; MacVille Merryn V. E.,NLX ; Hopman Anton H. N.,NLX, Methods for assessing genetic and phenotypic markers by simultaneous multicolor visualization of chromogenic dyes using brightfield microscopy and spectral imaging.
Zhang Linqi ; Lewin Sharon R.,AUX ; Kostrikis Leondios ; Ho David D., Methods for identifying genomic equivalent markers and their use in quantitating cells and polynucleotide sequences therein.
Heller Michael J. (Encinitas CA) Tu Eugene (San Diego CA) Butler William F. (Carlsbad CA), Molecular biological diagnostic systems including electrodes.
Collins George L. (Maplewood NJ) Zema Paul (Roselle Park NJ) Rashada Yusuf A. (Atlanta GA), Novel elastomer/oxymethylene polymer blends containing polymeric compatibilizing agents.
David J. Lockhart ; Mark Chee ; Kevin Gunderson ; Lai Chaoqiang ; Lisa Wodicka ; Maureen T. Cronin ; Danny Lee ; Huu M. Tran ; Hajime Matsuzaki, Nucleic acid analysis techniques.
Caruthers Marvin H. (Boulder CO) Brill Wolfgang K. D. (Schopfheim DEX) Yau Eric (Mercer Island WA) Ma Michael (New York NY) Nielsen John (Horsholm DKX), Nucleoside thiophosphoramidites.
Hollis Mark A. ; Ehrlich Daniel J. ; Murphy R. Allen ; Kosicki Bernard B. ; Rathman Dennis D. ; Mathews Richard H. ; Burke Barry E. ; Eggers Mitch D. ; Hogan Michael E. ; Varma Rajender Singh, Optical and electrical methods and apparatus for molecule detection.
Mullis Kary B. (Kensington CA) Erlich Henry A. (Oakland CA) Arnheim Norman (Woodland Hills CA) Horn Glenn T. (Emeryville CA) Saiki Randall K. (Richmond CA) Scharf Stephen J. (Berkeley CA), Process for amplifying, detecting, and/or-cloning nucleic acid sequences.
Rey-Senelonge Arielle (Rillieux la Pape FRX) Kohen Gilla (Rillieux la Pape FRX), Recombinant herpesviruses, in particular for the production of vaccines, process for preparing them, plasmids produced d.
Chu Barbara (Del Mar CA) Kramer Fred R. (New York NY) Lizardi Paul (Cuernavaca CA MXX) Orgel Leslie E. (La Jolla CA), Replicative RNA reporter systems.
Okano Kazunori (Shiki JPX) Kambara Hideki (Hachiouji JPX), Separation of polynucleotides using supports having a plurality of electrode-containing cells.
Bao Yijia ; Che Diping ; Li Wan-Liang ; Muller Uwe Richard ; Seelig Steven A. ; Shi Jufang, Simultaneous measurement of gene expression and genomic abnormalities using nucleic acid microarrays.
Goodwin Peter M. ; Jett James H. ; Keller Richard A. ; Van Orden Alan K. ; Machara Nicholas P., Single molecule identification using selected fluorescence characteristics.
Van Ness Jeffrey (Bothell WA) Petrie Charles R. (Woodinville WA) Tabone John C. (Bothell WA) Vermeulen Nicolaas M. J. (Woodinville WA), Solid supports for nucleic acid hybridization assays.
Renzoni George E. (Seattle WA) Schindele Deborah C. (Seattle WA) Theodore Louis J. (Seattle WA) Leznoff Clifford C. (North York WA CAX) Fearon Karen L. (Woodinville WA) Pepich Barry V. (Seattle WA), Tetrabenztriazaporphyrin reagents and kits containing the same.
Lindsay Stuart M. (Tempe AZ) Jing Tianwei (Tempe AZ) Lyubchenko Yuri L. (Tempe AZ) Gall Alexander A. (Bothell WA), Tip etching system and method for etching platinum-containing wire.
Demarest Keith (Flemington NJ) Schwender Charles (Califon NJ) Wustrow David (Ann Arbor MI), Trifluoromethybenzylphosphonates useful in treating osteoporosis.
Parikh, Bhairavi; Brody, Michael D.; Stone, James; Halderman, Jonathan D., Method and device for identification of nucleated red blood cells from a maternal blood sample.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.