초록 ▼

The present invention is directed to the use of a light absorbing wall material to eliminate stray light paths in light-guiding structures, such as those used for HPLC absorbance detection. More specifically, the present invention relates to the use of carbon-doped Teflon�� AF, or "black Teflon�� AF

The present invention is directed to the use of a light absorbing wall material to eliminate stray light paths in light-guiding structures, such as those used for HPLC absorbance detection. More specifically, the present invention relates to the use of carbon-doped Teflon�� AF, or "black Teflon�� AF," for all or part of the walls of a light-guiding flowcell adapted for use in HPLC absorbance detection.

대표청구항 ▼

The invention claimed is: 1. A set of light-guiding flowcells for a fluid comprising: a set of channels formed in a substrate of material having an index of refraction lower than an index of said fluid and an absorption coefficient sufficient to substantially attenuate light propagating through sai

The invention claimed is: 1. A set of light-guiding flowcells for a fluid comprising: a set of channels formed in a substrate of material having an index of refraction lower than an index of said fluid and an absorption coefficient sufficient to substantially attenuate light propagating through said material; a set of channel covers formed in a section of material having an index of refraction identical to said substrate material and an absorption coefficient sufficient to substantially attenuate light propagating through said material, said set of channel covers fixed to said set of channels forming a set of covered channels; at least one covered channel having a fluid inlet and outlet port configured off an axis of said covered channel; at least one covered channel having a source of light at a light entrance end and having a light exit end; whereby said at least one covered channel and fluid effect guiding of said light by attenuated total reflection and said at least one covered channel has minimal absorptive effect on said light guided by internal reflection. 2. The set of light-guiding flowcells of claim 1 further comprising an optical fiber bonded to at least one covered channel providing said light source of light. 3. The set of light-guiding flowcells of claim 1 whereby the material forming said set of covered channels prevents light crosstalk between flowcells. 4. The set of light-guiding flowcells of claim 1 wherein at least one covered channel is configured as a separation column. 5. The set of light-guiding flowcells of claim 1 wherein at least one covered channel is configured as a fluidic connection. 6. The set of light-guiding flowcells of claim 1 wherein said substrate and said set of covered channels are formed of an amorphous fluoropolymer doped with a black dopant at a concentration of between 0.01-0.1% of the fluoropolymer. 7. The set of light-guiding flow cells of claim 6 wherein the back dopant is carbon black.