IPC분류정보
국가/구분 |
United States(US) Patent
등록
|
국제특허분류(IPC7판) |
|
출원번호 |
US-0614644
(2003-07-07)
|
등록번호 |
US-7390484
(2008-06-24)
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발명자
/ 주소 |
- Fraser,John K.
- Hedrick,Marc H.
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출원인 / 주소 |
- Cytori Therapeutics, Inc.
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대리인 / 주소 |
Knobbe Martens Olson & Bear LLP
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인용정보 |
피인용 횟수 :
73 인용 특허 :
41 |
초록
▼
Cells present in processed lipoaspirate tissue are used to treat patients. Methods of treating patients include processing adipose tissue to deliver a concentrated amount of stem cells obtained from the adipose tissue to a patient. The methods may be practiced in a closed system so that the stem cel
Cells present in processed lipoaspirate tissue are used to treat patients. Methods of treating patients include processing adipose tissue to deliver a concentrated amount of stem cells obtained from the adipose tissue to a patient. The methods may be practiced in a closed system so that the stem cells are not exposed to an external environment prior to being administered to a patient. Compositions that are administered to a patient include a mixture of adipose tissue and stem cells so that the composition has a higher concentration of stem cells than when the adipose tissue was removed from the patient.
대표청구항
▼
What is claimed is: 1. A self-contained adipose-derived stem cell processing unit, comprising: a tissue collection container that is configured to receive unprocessed adipose tissue that is removed from a patient, wherein said tissue collection chamber is defined by a closed system; a first filter
What is claimed is: 1. A self-contained adipose-derived stem cell processing unit, comprising: a tissue collection container that is configured to receive unprocessed adipose tissue that is removed from a patient, wherein said tissue collection chamber is defined by a closed system; a first filter that is disposed within said tissue collection container, wherein said first filter is configured to retain a first component of said unprocessed adipose tissue and pass a second component of said unprocessed adipose tissue, such that said first filter separates said first component from said second component, and wherein said first component comprises a cell population that comprises adipose-derived stem cells and said second component comprises lipid, blood, mature adipocytes, and saline; a cell collection chamber, which is configured to receive said first component comprising a cell population that comprises adipose-derived stem cells from said tissue collection container, wherein said cell collection container is within said closed system; a conduit configured to allow passage of said first component comprising a cell population comprising adipose-derived stem cells from said tissue collection chamber to said cell collection container while maintaining a closed system; a cell concentrator disposed within said cell collection chamber, which is configured to facilitate the concentration of said first component comprising a cell population that comprises adipose-derived stem cells so as to obtain a concentrated population of cells that comprise adipose-derived stem cells, wherein said cell concentrator comprises a centrifuge or a spinning membrane filter; and an outlet configured to allow the aseptic removal of said concentrated population of cells that comprise adipose-derived stem cells. 2. The cell processing unit of claim 1, wherein said tissue collection container is configured to receive lipoaspirate. 3. The cell processing unit of claim 2, wherein said tissue collection container is coupled to a cannula. 4. The cell processing unit of claim 2, wherein said tissue collection container is coupled to a suction device. 5. The cell processing unit of claim 1, wherein said tissue collection container is configured to receive adipose tissue that is removed by excisional lipectomy. 6. The cell processing unit of claim 1, wherein said first filter has a pore size of about 20 μm to about 5 mm. 7. The cell processing unit of claim 6, wherein said first filter is a mesh with a pore size of about 265 μm. 8. The cell processing unit of claim 1, wherein said tissue collection container is configured to receive an additive. 9. The cell processing unit of claim 8, wherein said tissue collection container is configured to agitate said adipose tissue and said additive. 10. The cell processing unit of claim 1, wherein said adipose-derived stem cell processing unit comprises a centrifuge. 11. The cell processing unit of claim 1, wherein said cell collection container comprises a spinning membrane filter. 12. The cell processing unit of claim 1, further comprising a second filter configured to pass said population of cells that comprises adipose stem cells and retain material larger than said population of cells. 13. The cell processing unit of claim 12, wherein said second filter is within said cell collection container. 14. The cell processing unit of claim 1, wherein said cell collection container is configured to receive an additive. 15. The cell processing unit of claim 1, wherein said cell collection container is configured to wash said concentrated population of cells that comprise adipose-derived stem cells. 16. The cell processing unit of claim 1, wherein the cell collection container is configured to separate said concentrated population of cells that comprise adipose-derived stem cells based on a difference in electrical charge. 17. The cell processing unit of claim 1, wherein the cell collection container further comprises a means to agitate said concentrated population of cells that comprise adipose-derived stem cells. 18. The cell processing unit of claim 14, wherein the cell collection container further comprises a means to agitate said concentrated population of cells that comprise adipose-derived stem cells. 19. The cell processing unit of claim 1, wherein the cell collection container further comprises a means for washing said concentrated population of cells that comprise adipose-derived stem cells. 20. The cell processing unit of claim 14, wherein the cell collection container further comprises a means for washing said concentrated population of cells that comprise adipose-derived stem cells. 21. The cell processing unit of claim 1, wherein said cell collection container comprises a means to separate said population of cells that comprise adipose-derived stem cells by density gradient. 22. The cell processing unit of claim 1, wherein said cell collection container comprises a means to separate said population of cells that comprise adipose-derived stem cells by adherence to plastic. 23. The cell processing unit of claim 1, wherein said cell collection container comprises a means to culture cells. 24. The cell processing unit of claim 1, further comprising a mixing container, wherein said mixing container is joined to said tissue collection container via a conduit configured to maintain a closed system, which wherein said mixing container comprises a port for the addition of an additive, wherein said mixing container is within said closed system, and wherein said cell collection container is positioned between said tissue collection container and said mixing chamber. 25. The cell processing unit of claim 24, wherein said second filter is positioned between said cell collection container and said mixing container. 26. The cell processing unit of claim 25, wherein said second filter has a pore size of between 20 μm and 200 μm. 27. The cell processing unit of claim 1, further comprising a temperature controller.
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