Transgenic algae for delivering antigens to an animal
원문보기
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
A01N-063/00
A61K-039/00
C12P-021/06
C12N-005/00
C12N-015/00
C12N-015/82
출원번호
US-0311741
(2001-06-20)
등록번호
US-7410637
(2008-08-12)
국제출원번호
PCT/US01/019643
(2001-06-20)
§371/§102 date
20021218
(20021218)
국제공개번호
WO01/098335
(2001-12-27)
발명자
/ 주소
Sayre,Richard T.
Wagner,Richard E.
Siripornadulsil,Surasak
Farias,Carlos
출원인 / 주소
Phycotransgenics, LLC
The Ohio State University
대리인 / 주소
Barnes & Thornburg, LLP
인용정보
피인용 횟수 :
23인용 특허 :
13
초록▼
Delivery systems and methods are provided for delivering a biologically active protein to a host animal. The systems and methods provided include obtaining an algal cell transformed by an expression vector, the expression vector comprising a nucleotide sequence coding for the biologically active pro
Delivery systems and methods are provided for delivering a biologically active protein to a host animal. The systems and methods provided include obtaining an algal cell transformed by an expression vector, the expression vector comprising a nucleotide sequence coding for the biologically active protein, operably linked to a promoter. In one illustrated embodiment, the biologically active protein is an antigenic epitope and upon administration to the animal the algal cell induces an immune response in the host animal.
대표청구항▼
The invention claimed is: 1. A delivery system for delivering a biologically active protein to a host animal comprising an algal cell transformed by an expression vector, the expression vector comprising a β-tubulin promoter of Chlamydomonas, a 3'-terminator from the Chlamydomonas low C2-induc
The invention claimed is: 1. A delivery system for delivering a biologically active protein to a host animal comprising an algal cell transformed by an expression vector, the expression vector comprising a β-tubulin promoter of Chlamydomonas, a 3'-terminator from the Chlamydomonas low C2-induced membrane protein gene having the sequence of SEQ ID NO. 4, and a nucleotide sequence coding for the biologically active protein fused to an end of the low CO2-induced plasma membrane protein gene SEQ ID NO. 3, said fused nucleotide sequence positioned between the β-tubulin promoter and the 3' terminator and operably linked to the β-tubulin promoter. 2. The delivery system of claim 1, wherein the algal cell is suspended in water for immersing the host animal. 3. The delivery system of claim 1 wherein the biologically active protein is selected from the group consisting of hormones and antimicrobial peptides. 4. The delivery system of claim 1 wherein the algal cell is mixed with a sample of feed for the host animal. 5. The delivery system of claim 1 wherein the animal is selected from the group consisting of mammals, fish, birds, and crustaceans. 6. The delivery system of claim 1 wherein the biologically active protein is a peptide derived from the group consisting of bactericidal proteins, insecticidal proteins, growth hormones, and antigens. 7. The delivery system of claim 1, wherein the nucleotide sequence is cloned into a multicloning site of the expression vector, the multicloning site comprising NaeI, KpnI, Apal, XhoI, SalI, ClaI, HindIII, EcoRV, EcoRI, PstI, SmaI, BamHI, SpeI, XbaI, NotI, and NarI. 8. A method for delivering antigens to a host animal to induce an immune response in the host animal, the method comprising the steps of administering an immunogenic composition to the host animal wherein the composition comprises i) an algal cell transformed by an expression vector, and ii) the expression vector comprising a nucleotide sequence coding for an antigen comprising a holoprotein or protein fragment from a microorganism selected from the group consisting of a bacterium, a virus, a fungus, and a parasite, and inducing the immune response in the host animal. 9. The method of claim 8, wherein the expression vector further comprises a promoter operably linked to the nucleotide sequence coding for the antigen. 10. The method of claim 9, wherein the expression vector further comprises a terminator for terminating transcription. 11. The method of claim 10, wherein the algal cell expresses the antigen in an area selected from the group consisting of nucleus, chloroplast, mitochondria, periplasmic space, cell membrane, cytoplasm, and cell wall. 12. The method of claim 8, wherein the algal cell is selected from the group consisting of green algae, brown algae, and diatoms. 13. The method of claim 8 wherein the algal cell is Chlamydomonas reinhardtii. 14. The method of claim 8 wherein the antigen is part of a fusion protein. 15. The method of claim 8 wherein the algal cell is disabled. 16. The method of claim 8 wherein the algal cell is packaged as a food product. 17. The method of claim 16 wherein the food product is a capsule. 18. The method of claim 8 wherein multiple algal cells are incorporated into a pellet. 19. The method of claim 18 wherein the algal cells are selected from the group consisting of living and dead cells. 20. The method of claim 8 wherein the algal cell is suspended in water for immersing the host animal. 21. The method of claim 20 wherein the algal cell is dried and formed into a powder prior to being suspended in water. 22. The method of claim 8 wherein the algal cell is mixed with a liquid and packaged as a drink. 23. The method of claim 8, wherein the expression vector further comprises a 5' psbA promoter and a 3' psbA terminator, and wherein the nucleotide sequence coding for the antigen is positioned between the 5' psbA promoter and the 3' psbA terminator and is operably linked to the 5' psbA promoter. 24. The method of claim 8 wherein the nucleotide sequence coding for the antigen is fused to an end of the low CO2-induced plasma membrane protein gene. 25. The method of claim 8 wherein the expression vector is incorporated into genetic material selected from the group consisting of nuclear, chloroplast, and mitochondrial. 26. The delivery system of claim 1 wherein the algal cell is delivered by oral administration. 27. The delivery system of claim 5 wherein the algal cell is delivered by oral administration. 28. The delivery system of claim 5, wherein the animal is a fish or a crustacean and the algal cell is suspended in water for immersing the host animal. 29. The method of claim 8 wherein the algal cell is delivered by oral administration. 30. The method of claim 8 wherein the animal is selected from the group consisting of mammals, fish, birds, and crustaceans. 31. The method of claim 30 wherein the algal cell is delivered by oral administration. 32. The method of claim 30, wherein the animal is a fish or a crustacean and the algal cell is suspended in water for immersing the host animal.
연구과제 타임라인
LOADING...
LOADING...
LOADING...
LOADING...
LOADING...
이 특허에 인용된 특허 (13)
Hagiwara Hideaki,JPX ; Takeshima Yasunobu,JPX, Expression of human SOD in blue green algae.
Cramer Carole Lyn (Blacksburg VA) Weissenborn Deborah Louise (Blacksburg VA), HMG2 promoter expression system and post-harvest production of gene products in plants and plant cell cultures.
Dunahay Terri Goodman (2710 Arbor Glen Pl. Boulder CO 80304) Roessler Paul G. (15905 Ellsworth Pl. Golden CO 80401) Jarvis Eric E. (3720 Smuggler Pl. Boulder CO 80303), Method to transform algae, materials therefor, and products produced thereby.
Lowe Robert ; Joyce Joseph G. ; George Hugh A. ; Hofmann Kathryn J. ; Jansen Kathrin U. ; Lehman E. Dale ; Neeper Michael P., Production of recombinant human papillomavirus type II protein utilizing papillomavirus 6/11 hybrid DNA.
Loy, John Dustin; Bartholomay, Lyric Colleen; Harris, Delbert Linn; Scura, Ed; Kamrud, Kurt Iver, Methods and compositions to protect aquatic invertebrates from disease.
Loy, John Dustin; Bartholomay, Lyric Colleen; Harris, Delbert Linn; Scura, Ed; Kamrud, Kurt Iver, Methods and compositions to protect aquatic invertebrates from disease.
Loy, John Dustin; Bartholomay, Lyric Colleen; Harris, Delbert Linn; Scura, Ed; Kamrud, Kurt Iver, Methods and compositions to protect aquatic invertebrates from disease.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.