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Methods and reagents for combined PCR amplification

IPC분류정보
국가/구분 United States(US) Patent 등록
국제특허분류(IPC7판)
  • B01L-003/00
  • C07H-021/04
  • C07H-021/00
  • C12Q-001/68
  • C12P-019/34
  • C12P-019/00
출원번호 US-0775151 (2007-07-09)
등록번호 US-7413708 (2008-08-19)
발명자 / 주소
  • Mayrand,Paul E.
출원인 / 주소
  • Applied Biosystems Inc.
대리인 / 주소
    Bortner,Scott R.
인용정보 피인용 횟수 : 7  인용 특허 : 27

초록

An oligonucleotide probe is disclosed, the probe including an oligonucleotide, a fluorescer molecule attached to a first end of the oligonucleotide and a quencher molecule attached to the opposite end of the oligonucleotide. The probe is rendered impervious to digestion by the 5'→3' exonucleas

대표청구항

I claim: 1. A kit for performing a combined PCR amplification and hybridization probing comprising: (a) an oligonucleotide probe comprising: an oligonucleotide capable of hybridizing to a target polynucleotide sequence; a fluorescer molecule attached to a first location on the oligonucleotide; a qu

이 특허에 인용된 특허 (27)

  1. Harris Raymond John,AUX ; Morris Charles Phillip,AUX, Amplification and detection process.
  2. Goodman Thomas C. (Mountain View CA) Becker Martin (Palo Alto CA) Ullman Edwin F. (Atherton CA) Rose Samuel (Mountain View CA), Amplification method for polynucleotide detection assays.
  3. Maggio Edward T. (Redwood City CA), Chemically induced fluorescence immunoassay.
  4. Tyagi Sanjay ; Kramer Fred R. ; Lizardi Paul M.,MXX, Detectably labeled dual conformation oligonucleotide probes, assays and kits.
  5. Tyagi Sanjay ; Kramer Fred R. ; Lizardi Paul M.,MXX, Detectably labeled, dual conformation oligonucleotide probes, assays and kits.
  6. Sorenson George D., Detection of soluble gene sequences in biological fluids.
  7. Gelfand David H. ; Holland Pamela M. ; Saiki Randall K. ; Watson Robert M., Homogeneous assay system.
  8. Gelfand David H. (Oakland CA) Holland Pamela M. (Oakland CA) Saiki Randall K. (Richmond CA) Watson Robert M. (Berkeley CA), Homogeneous assay system using the nuclease activity of a nucleic acid polymerase.
  9. Livak Kenneth J. ; Flood Susan J. A. ; Mamoro Jeffrey ; Mullah Khairuzzaman Bashar, Hybridization assay using self-quenching fluorescence probe.
  10. Livak Kenneth J. ; Flood Susan J. A. ; Marmaro Jeffrey ; Mullah Khairuzzaman Bashar, Hybridization assay using self-quenching fluorescence probe.
  11. Atwood John G. (West Redding CT) Haff Lawrence A. (Wilton CT), In situ PCR amplification system.
  12. Kidwell David A. (Alexandria VA), Light emission-or absorbance-based binding assays for polynucleic acids.
  13. Okamoto Tadashi,JPX ; Tomida Yoshinori,JPX ; Yamamoto Nobuko,JPX ; Kawaguchi Masahiro,JPX ; Makino Keisuke,JPX ; Murakami Akira,JPX, Method for detecting a target nucleic acid by using an interaction of two kinds of reagents.
  14. Livak Kenneth J. (San Jose CA) Flood Susan J. A. (Fremont CA) Marmaro Jeffrey (Foster City CA), Method for detecting nucleic acid amplification using self-quenching fluorescence probe.
  15. Demers Daniel B. (Broad Run VA), Method for enhancing amplification in the polymerase chain reaction employing peptide nucleic acid (PNA).
  16. Demers Daniel B. (Broad Run VA), Method for enhancing amplification in the polymerase chain reaction employing peptide nucleic acid (PNA).
  17. Paul E. Mayrand, Methods and reagents for combined PCR amplification and hybridization probing.
  18. Mayrand Paul E. (Pacifica CA), Methods for combined PCR amplification and hybridization probing using doubly labeled fluorescent probes.
  19. Erlich Henry A. (Oakland CA) Higuchi Russell G. (San Francisco CA), Methods for nucleic acid amplification.
  20. Stanley John Christopher,GBX, Nucleic acid analogue assay procedures and kits.
  21. Gelfand David H. (Oakland CA) Holland Pamela M. (Seattle WA) Saiki Randall K. (Richmond CA) Watson Robert M. (Berkeley CA), Nucleic acid detection by the 5′-3′exonuclease activity of polymerases acting on adjacently hybridized oligonucleotide.
  22. Link John R. (Springfield VA) Gudibande Satyanarayana R. (Rockville MD) Kenten John H. (Gaithersburg MD), Rapid assays for amplification products.
  23. Gelfand David H. ; Holland Pamela M. ; Saiki Randall K. ; Watson Robert M., Reaction mixtures for detection of target nucleic acids.
  24. Livak Kenneth J. ; Flood Susan J.A. ; Marmaro Jeffrey ; Mullah Khairuzzaman Bashar, Self-quenching fluorescence probe.
  25. Walker George T. (Chapel Hill NC) Nadeau James G. (Chapel Hill NC) Little Michael C. (Raleigh NC), Simultaneous amplification of multiple targets.
  26. Buchardt ; deceased Ole,DKX ; Buchardt ; legal representative by Dorte,DKX ; Egholm Michael ; Nielsen Peter Eigil,DKX ; Berg Rolf Henrik,DKX ITX DK-2000 ; Stanley Christopher John,GBX ITX 4JB, Use of nucleic acid analogues in the inhibition of nucleic acid amplification.
  27. Buchardt ; deceased Ole,DKX ITX by Dante Buchardt legal representative ; Egholm Michael ; Nielsen Peter Eigil,DKX ; Berg Rolf Henrik,DKX ; Stanley Christopher John,GB3, Use of nucleic acid analogues in the inhibition of nucleic acid amplification.

이 특허를 인용한 특허 (7)

  1. Akirav, Eitan Moshe, Method for using probe based PCR detection to measure the levels of circulating demethylated beta cell derived DNA as a measure of beta cell loss in diabetes.
  2. Akirav, Eitan Moshe, Method for using probe based PCR detection to measure the levels of circulating demethylated beta cell derived DNA as a measure of beta cell loss in diabetes.
  3. Akirav, Eitan Moshe, Method for using probe based PCR detection to measure the levels of circulating demethylated β cell derived DNA as a measure of β cell loss in diabetes.
  4. Liu, Guozhen; Peng, Quan; Wang, Yexun; Rulli, Samuel J.; Lo, Jing-Yi; Gardner, Rachel; Kim, Grace J., Methods and compositions for nucleic acid detection.
  5. Rajagopal, Aditya; Kartalov, Emil P., Nucleic acid reactions and related methods and compositions.
  6. Kartalov, Emil; Rajagopal, Aditya; Scherer, Axel, Signal encoding and decoding in multiplexed biochemical assays.
  7. Kartalov, Emil; Rajagopal, Aditya; Scherer, Axel, Signal encoding and decoding in multiplexed biochemical assays.
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