초록 ▼

The invention described herein provides methods for the detection of soluble antigens. In particular, the methods provide for the detection of soluble proteins and chemicals. In addition, the invention provides methods of detecting a nucleic acid sequence in a sample. Also described is an emittor ce

The invention described herein provides methods for the detection of soluble antigens. In particular, the methods provide for the detection of soluble proteins and chemicals. In addition, the invention provides methods of detecting a nucleic acid sequence in a sample. Also described is an emittor cell comprising an Fc receptor and an emittor molecule for the detection of a target particle in a sample wherein the target particle to be detected is bound by one or more antibodies. Also provided is an optoelectronic sensor device for detecting a target particle in a plurality of samples.

대표청구항 ▼

What is claimed is: 1. A method of detecting a soluble antigen in a sample comprising the steps of: a) crosslinking the soluble antigen, thereby producing a crosslinked antigen; b) combining the crosslinked antigen with an emittor cell comprising an antibody that binds to the crosslinked antigen, w

What is claimed is: 1. A method of detecting a soluble antigen in a sample comprising the steps of: a) crosslinking the soluble antigen, thereby producing a crosslinked antigen; b) combining the crosslinked antigen with an emittor cell comprising an antibody that binds to the crosslinked antigen, wherein binding of the antibody to the crosslinked antigen results in an increase in intracellular calcium, and wherein said emittor cell further comprises an emittor molecule that emits a photon in response to the increase in intracellular calcium; and c) measuring photon emission from the emittor cell, thereby detecting a soluble antigen in a sample. 2. The method of claim 1, wherein, the antibody that binds to the crosslinked antigen is selected from the group consisting of a chimeric antibody, a single-chain antibody, a monoclonal antibody and a polyclonal antibody. 3. The method of claim 1, wherein, the soluble antigen is selected from the group consisting of a protein and a chemical. 4. The method of claim 1, wherein the soluble antigen is crosslinked using one or more agents selected from the group consisting of molecules, antibodies, chemical compounds and ligands. 5. The method of claim 4, wherein the one or more agents crosslink the soluble antigen by precipatation of the soluble antigen or by attachment of the soluble antigen to a solid phase. 6. The method of claim 1, wherein the emittor molecule is a calcium-sensitive luminescent molecule or a calcium-sensitive fluorescent molecule. 7. The method of claim 6, wherein the emittor molecule is selected from the group consisting of aequorin, obelin, thalassicolin, mitrocomin, clytin, mnemiopsin, berovin, Indo-1, Fura-2, Quin-2, Fluo-3, Rhod-2, calcium green, BAPTA, a cameleon and combinations thereof. 8. The method of claim 1, wherein the emittor cell is a cell selected from the group consisting of a prokaryotic cell, a eukaryotic cell and a non-living cell. 9. The method of claim 8, wherein the emittor cell is selected from the group consisting of a B-cell, a T-cell, a macrophage cell, a mast cell and a fibroblast. 10. A method of detecting a soluble antigen in a sample comprising the steps of: a) crosslinking the soluble antigen to a solid substrate, thereby producing a crosslinked soluble antigen bound to a solid substrate; b) adding an emittor cell to the crosslinked soluble antigen bound to the solid substrate, wherein said emittor cell comprises an antibody that binds an epitope on the soluble antigen, wherein binding of the antibody to the crosslinked soluble antigen bound to the solid support results in an increase in intracellular calcium, and wherein said emittor cell further comprises an emittor molecule that emits a photon in response to the increase in intracellular calcium; and c) measuring photon emission from the emittor cell, thereby detecting a soluble antigen in a sample. 11. The method of claim 10, wherein the soluble antigen is a protein. 12. The method of claim 10, wherein the soluble antigen is a chemical. 13. The method of claim 10, wherein the soluble antigen is crosslinked to the solid substrate using one or more agents selected from the group consisting of molecules, antibodies, chemical compounds and ligands. 14. The method of claim 10, wherein the solid substrate comprises one or more substrates selected from the group consisting of a multi-well plate, a microcentrifuge tube, a filter unit, a bead, a cell, a charged molecule and a bacterium. 15. The method of claim 10, wherein, the antibody that binds an epitope on the soluble antigen is selected from the group consisting of a chimeric antibody, a single-chain antibody, a monoclonal antibody and a polyclonal antibody. 16. The method of claim 10, wherein the emittor molecule comprises a calcium-sensitive luminescent molecule or a calcium-sensitive fluorescent molecule. 17. The method of claim 16, wherein the emittor molecule is selected from the group consisting of aequorin, obelin, thalassicolin, mitrocomin (halistaurin), clytin (phialidin), mnemiopsin, berovin, Indo-1, Fura-2, Quin-2, Fluo-3, Rhod-2, calcium green, BAPTA, a cameleon and combinations thereof. 18. The method of claim 10, wherein the emittor cell comprises a cell selected from the group consisting of a prokaryotic cell, a eukaryotic cell and a non-living cell. 19. The method of claim 18, wherein the emittor cell is selected from the group consisting of a B-cell, a T-cell, a macrophage cell, a mast cell and a fibroblast.