초록

DNA encoding a plant quinolate phosphoribosyl transferase (QPRTase) enzyme, and constructs comprising such DNA are provided. Methods of altering quinolate phosphoribosyl transferase expression are provided.

대표청구항 ▼

That which is claimed: 1. A transgenic tobacco plant of the genus Nicotiana having reduced quinolate phosphoribosyl transferase (QPRTase) production relative to a non-transformed control plant, said transgenic plant comprising transgenic plant cells containing a heterologous nucleic acid comprising

That which is claimed: 1. A transgenic tobacco plant of the genus Nicotiana having reduced quinolate phosphoribosyl transferase (QPRTase) production relative to a non-transformed control plant, said transgenic plant comprising transgenic plant cells containing a heterologous nucleic acid comprising a nucleotide sequence selected from the group consisting of: (a) the nucleotide sequence of SEQ ID NO: 1; (b) a nucleotide sequence that encodes a quinolate phosphoribosyl transferase enzyme having the amino acid sequence of SEQ ID NO:2; and (c) a nucleotide sequence that is at least 95% identical to the nucleotide sequence of (a) or (b) above and that encodes a quinolate phosphoribosyl transferase enzyme, and wherein said transgenic plant further comprises a transgene encoding a heterologous protein of interest. 2. The transgenic tobacco plant of claim 1, wherein the heterologous protein of interest is a Bacillus thuringiensis insecticidal protein. 3. A method of producing a heterologous protein of interest in a plant, comprising: a) growing the transgenic tobacco plant of claim 1; and b) collecting the heterologous protein of interest from said transgenic tobacco plant. 4. The method of claim 3, wherein the heterologous protein of interest is a Bacillus thuringiensis insecticidal protein. 5. The transgenic tobacco plant of claim 1, wherein said heterologous nucleic acid is in antisense orientation. 6. The transgenic tobacco plant of claim 1, wherein said heterologous nucleic acid is in sense orientation. 7. The method of claim 3, wherein said heterologous nucleic acid is in antisense orientation. 8. The method of claim 3, wherein said heterologous nucleic acid is in sense orientation.