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A mixing vessel contains a septum, dividing a first chamber from a second chamber. By relatively moving the septum with respect to the ends of the vessel, and flowing a fluid through the septum, the fluid is treated as it moves from the first chamber to the second chamber. The particular configurati

A mixing vessel contains a septum, dividing a first chamber from a second chamber. By relatively moving the septum with respect to the ends of the vessel, and flowing a fluid through the septum, the fluid is treated as it moves from the first chamber to the second chamber. The particular configuration of the septum allows for treatments such as mixing, reacting, heating, and cooling, as well as filtering of the fluid.

대표청구항 ▼

We claim: 1. A vessel comprising a septum spaced intermediate a first and second opposite ends, said septum being attached to at least one wall of said vessel so as to divide the vessel into at least two chambers and having an open area expressed as percentage of the surface, between 10 to 90%, whe

We claim: 1. A vessel comprising a septum spaced intermediate a first and second opposite ends, said septum being attached to at least one wall of said vessel so as to divide the vessel into at least two chambers and having an open area expressed as percentage of the surface, between 10 to 90%, wherein at least one of said first and second ends comprises a piston, said piston arranged to moved said first or second chambers. 2. The vessel of claim 1, wherein said vessel comprises at least one port for introducing or removing components. 3. The vessel of claim 1, wherein said septum is relatively moveable with respect to said first and second ends. 4. The vessel of claim 1, wherein the septum comprises at least one element selected from the group consisting of micropores, a vacuum device, and moveable tubes embedded therein. 5. The vessel of claim 1, wherein each of said first and second ends comprise a piston. 6. The vessel of claim 1, wherein the septum is formed from at least one material selected from the group consisting of fabric, metal, plastic and ceramic. 7. The vessel of claim 1, wherein the septum is rigid. 8. The vessel of claim 1, wherein said septum has an open area, as a percentage of total area of said septum, between approximately 30 and approximately 75%. 9. A device comprising the vessel of claim 1, wherein the vessel is cylindrical. 10. A vessel comprising a septum spaced intermediate first and second opposite ends, said septum being attached to at least one wall of said vessel so as to divide the vessel into at least two chambers, wherein at least one of said first and second ends comprises a piston, further comprising at least one perfusion filter positioned at least one of the chambers in said vessel. 11. A method comprising: repeatedly relatively passing a fluid from a first chamber to a second chamber in a vessel, including passing said fluid through a septum by moving said septum with respect to ends of said first and second chambers, by a piston provided at least at one end of the vessel and moving an end of at least one of said first and second chambers. 12. The method of claim 11, further comprising removing gas from at least one of the first chamber and the second chamber. 13. The method of claim 11, further comprising introducing an inert atmosphere to at least one of the first chamber and the second chamber. 14. The method of claim 11, wherein said method is at least one selected from the group consisting of mixing, reacting, fermenting, filtering, sterilizing, lysing and ion exchanging. 15. The method of claim 11, wherein said passing comprises moving the septum through the fluid. 16. The method of claim 11, wherein said passing comprises moving at least one piston to provide pressure to said fluid. 17. The method of claim 16, wherein said pressure is a negative pressure. 18. The method of claim 16, wherein said passing comprises moving two pistons to provide pressure to said fluid. 19. A method comprising: repeatedly relatively passing a fluid from a first chamber to a second chamber in a vessel, including passing said fluid through a septum by moving said septum with respect to ends of said first and second chambers said septum is attached to at least one wall of the vessel, the method further comprising at least one step of heating and cooling the fluid in the vessel. 20. The method of claim 19, wherein said heating or cooling comprises passing a heating or cooling fluid through the septum. 21. A method comprising: providing a vessel having a first and a second chambers divided by a septum attached to at least one wall of the vessel; introducing a fluid into the vessel; repeatedly relatively passing the fluid through the septum by moving the septum with respect to the end of said first and second chambers, and further introducing a second component into the vessel. 22. The method of claim 21 wherein the second component is a pigment.