Particle-mediated transformation of vertebrate tissue cells
원문보기
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
A01N-043/04
A01N-043/02
A61K-031/70
C12N-015/87
출원번호
US-0610651
(2003-07-02)
등록번호
US-7449449
(2008-11-11)
발명자
/ 주소
Johnston,Stephen A.
Sanford,John C.
출원인 / 주소
Duke University
Cornell University
대리인 / 주소
Crane,Sharon E.
인용정보
피인용 횟수 :
0인용 특허 :
18
초록▼
A method of transferring a gene to vertebrate cells is disclosed. The method comprises the steps of: (a) providing microprojectiles, the microprojectiles carrying polynucleic acid sequences, the sequences comprising, in the 5' to 3' direction, a regulatory sequence operable in the tissue cells and a
A method of transferring a gene to vertebrate cells is disclosed. The method comprises the steps of: (a) providing microprojectiles, the microprojectiles carrying polynucleic acid sequences, the sequences comprising, in the 5' to 3' direction, a regulatory sequence operable in the tissue cells and a gene positioned downstream of the regulatory sequence and under the transcriptional control thereof; and (b) accelerating the microprojectiles at the cells, with the microprojectiles contacting the cells at a speed sufficient to penetrate the cells and deposit the polynucleic acid sequences therein. Preferably, the target cells reside in situ in the animal subject when they are transformed. Preferred target cells are dermis or hypodermis cells, and preferred genes for insertion into the target cells are genes which code for proteins or peptides which produce a physiological response in the animal subject.
대표청구항▼
That which is claimed is: 1. An agent for genetically transforming tissue cells of a living vertebrate, said agent comprising microparticles coated with stabilizing agent and carrying a polynucleotide sequence, said polynucleotide sequence comprising, in the 5' to 3' direction, (1) a regulatory seq
That which is claimed is: 1. An agent for genetically transforming tissue cells of a living vertebrate, said agent comprising microparticles coated with stabilizing agent and carrying a polynucleotide sequence, said polynucleotide sequence comprising, in the 5' to 3' direction, (1) a regulatory sequence operable in vertebrate tissue; and positioned downstream of said regulatory sequence and under the transcriptional control thereof, (2) a nucleotide sequence encoding a protein or peptide immunogen from a vertebrate pathogen against which an immune response is desired, wherein upon expression of said nucleotide sequence encoding the protein or peptide immunogen in the tissue cells, said vertebrate exhibits an immune response to said protein or peptide immunogen. 2. An agent according to claim 1, wherein the microparticles are metallic. 3. An agent according to claim 1, wherein the microparticles are gold. 4. An agent according to claim 1, wherein the microparticles are a polymer material. 5. An agent according to claim 1, wherein the microparticles are a polymer material selected from the group of polyethylene, polypropylene, and polycarbonate. 6. An agent according to claim 1, wherein the microparticles have diameters of from 1 micron to 3 microns. 7. An agent according to claim 1, wherein the vertebrate tissue is muscle tissue. 8. An agent according to claim 1, wherein the vertebrate tissue is skin tissue. 9. An agent according to claim 1, wherein the skin tissue is selected from the group of dermis tissue and hypodermis tissue. 10. An agent according to claim 1, wherein the vertebrate is a human. 11. An agent according to claim 1, wherein the polynucleotide sequence comprises a further regulatory sequence selected from the group of enhancers, termination sequences, and polyadenylation sites. 12. An agent according to claim 1, wherein the regulatory sequence is operable in only one cell type in the vertebrate tissue. 13. An agent according to claim 1, wherein the nucleotide sequence encoding a protein or peptide immunogen codes for subunit vaccine. 14. An agent according to claim 13, wherein the nucleotide sequence encoding a protein or peptide immunogen codes for the surface antigen of the Hepatitis B virus. 15. An agent according to claim 1, wherein the polynucleotide sequence is immobilized on the microparticles by precipitation. 16. An agent according to claim 1, wherein the microparticles are coated with polylysine. 17. An agent according to claim 1, wherein the vertebrate pathogen is a virus.
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이 특허에 인용된 특허 (18)
Harnden Michael Raymond (Horsham EN), Biologically active material.
Ladner Robert C. (3827 Green Valley Iamsville MD 21754), Computer based system and method for determining and displaying possible chemical structures for converting double- or m.
Felgner Philip L. (Rancho Santa Fe CA) Wolff Jon A. (Madison WI) Rhodes Gary H. (Leucadia CA) Malone Robert W. (Chicago IL) Carson Dennis A. (Del Mar CA), Induction of a protective immune response in a mammal by injecting a DNA sequence.
Sanford John C. (Geneva) DeVit Michael J. (Geneva NY) Bruner Ronald F. (Sewell NJ) Johnston Stephen A. (Durham NC), Method and apparatus for introducing biological substances into living cells.
Sanford John C. (Geneva NY) Wolf Edward D. (Ithaca NY) Allen Nelson K. (Newfield NY), Method for transporting substances into living cells and tissues.
Sanford John C. (Geneva NY) Wolf Edward D. (Ithaca NY) Allen Nelson K. (Newfield NY), Method for transporting substances into living cells and tissues and apparatus therefor.
Szoka ; Jr. Francis C. (76 Summit St. Waltham MA 02154) Papahadjopoulos Demetrios P. (3170 Condit St. Lafayette CA 94549), Method of inserting DNA into living cells.
Evans Ronald M. (La Jolla CA) Palmiter Richard D. (Seattle WA) Brinster Ralph L. (Gladwyne PA), Method of obtaining gene product through the generation of transgenic animals.
Johnston Stephen A. ; Williams R. Sanders ; Sanford John C. ; McElligott Sandra G., Particle-mediated bombardment of DNA sequences into tissue to induce an immune response.
Viza Dimitri (Bourg-la-Reine FRX) Adamopoulos Dimitri (Athens GRX) Phillips John (L\Hay les Romes FRX), Production of specific immune nucleic acids cell dialysates and antibodies.
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