초록

A resonant high-speed microscopic impedance probe useful for small scale impedance measurements and/or cell and particle counting.

대표청구항 ▼

What is claimed is: 1. A device capable of measuring impedance variances in a small region comprising: a probe electrode separated by one or more non-conducting regions from one or more shield electrodes; a detecting end of said probe electrode that can be placed in close proximity to said small re

What is claimed is: 1. A device capable of measuring impedance variances in a small region comprising: a probe electrode separated by one or more non-conducting regions from one or more shield electrodes; a detecting end of said probe electrode that can be placed in close proximity to said small region; an inductor L operatively connected to said probe electrode and to a transmission conductor connecting to measurement electronics; a signal generator for generating a radiofrequency signal; and impedance measurement electronics able to measure a reflected signal and thereby determine impedance variances at said functional end. 2. The device of claim 1 further comprising: a varactor diode that acts as a controllable capacitance Cv operatively connected to said probe electrode, said inductor and to a transmission conductor connecting to measurement electronics. 3. The device of claim 2 further wherein: a stray capacitance between said probe and said one or more shield electrodes C resonates with said inductor L and said varactor diode forming a tunable LC resonant circuit with an approximate resonance frequency f0=(��)(1/L(C+CV))1/2 wherein said resonance frequency can be tuned by adjusting the effective capacitance Cv. 4. The device of claim 2 further comprising: a second inductor L2 operatively connected to said varactor and said inductor L and a DC source that allows separate DC tuning of the varactor diode (indicated as a variable capacitor CV) and RF reflectance; wherein inductor L2 allows a DC bias circuit to be isolated from an RF part of the device. 5. The device of claim 1 further wherein: said probe electrode, said detecting end, and said inductor L are configured in a planar geometry. 6. The device of claim 2 further wherein: said probe electrode, said detecting end, said inductor L, and said varactor are configured in a planar geometry. 7. The device of claim 1 further wherein: a stray capacitance between said probe and said one or more shield electrodes C resonates with said inductor L, forming an LC resonant circuit with an approximate resonance frequency f0=(��π)(1/LC)1/2. 8. The device of claim 1 further wherein: said inductor L includes a small series resistance r, thereby generating a measured reflectance loss with a peak centered at the resonance frequency f0 such that if a sample is placed near the detecting end of the probe, so that it couples to the electromagnetic fields emanating from the probe end, the effective capacitance and resistance of the sample impedance changes the reflectance loss. 9. The device of claim 8 further wherein: a capacitive part of the sample impedance changes the resonance frequency f0; and a resistive part of the sample impedance changes the magnitude of the reflection loss. 10. A method for measuring impedance variations in a small volume comprising: placing a microfabricated probe with a detecting end in proximity to said volume; and using a tank circuit to couple said microfabricated probe to radiofrequency circuitry; and impedance measurement electronics able to measure a reflected signal and thereby determine impedance variances at said functional end of said microfabricated probe. 11. The method of claim 10 further wherein: said tank circuit comprises a lumped inductor in parallel with a stray and optionally a lumped capacitance. 12. The method of claim 10 further wherein: said probe comprises a sharp tip analogous to a scanning tunneling microscope tip, with a metal shield surrounding it. 13. The method of claim 10 further wherein: said probe comprises a planar geometry with a ground-signal-ground arrangement. 14. The method of claim 10 further wherein: said tank circuit comprises: a variable capacitor diode ("varactor") that can function as an electronically controlled tuning element to improve impedance matching. 15. The method of claim 14 further wherein: said varactor is co-fabricated on a same substrate as said microfabricated probe. 16. The method of claim 10 further wherein: said probe and related radiofrequency electronics senses changes in absolute and/or real and/or imaginary part of impedance presented by a sample; said sample comprises one or more selected from the group comprising: a solid object, possibly with mechanical degrees of freedom; a fluid, possibly with ions or other molecules dissolved in it; and a surface on which objects or molecules attach themselves and are sensed or otherwise measured. 17. The method of claim 10 further wherein: a distance between said probe and a ground connection is adjustable, thereby adjusting the particle size sensitivity. 18. The device of claim 1 further wherein: said impedance measurement electronics and said probe are constructed to detect particles in a fluid. 19. The device of claim 18 further wherein: said particles can include biological cells or labeling beads, either dielectric or metal, or other particles contained in the fluid with different electrical properties from the fluid (including inhomogeneous mixtures of two or more fluids). 20. The device of claim 18 further wherein: said probe can be used to sense materials or objects attached to the particles. 21. The device of claim 18 further wherein: said probe can be used for one or more of: measuring flow rates; measuring concentrations of particles; distinguishing particles in a mixed fluid stream; and measuring diffusion rates of particles.