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특허 상세정보

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Direct differentiation method for making cardiomyocytes from human embryonic stem cells

특허상세정보
국가/구분 United States(US) Patent 등록
국제특허분류(IPC7판) C12N-005/00    C12N-005/02    C12N-005/06    C12N-005/10    C12N-005/08   
미국특허분류(USC) 435/377; 435/383; 435/384; 435/363; 435/366
출원번호 US-0086709 (2005-03-21)
등록번호 US-7452718 (2008-11-18)
발명자 / 주소
  • Gold,Joseph D.
  • Hassanipour,Mohammad
  • Collins,Lila R.
  • Xu,Chunhui
출원인 / 주소
  • Geron Corporation
대리인 / 주소
    Mittler,E. Stewart
인용정보 피인용 횟수 : 30  인용 특허 : 11
초록

This invention provides a new procedure for generating cardiomyocyte lineage cells from embryonic stem cells for use in regenerative medicine. Differentiating by way of embryoid body formation or in serum is no longer required. Instead, the stem cells are plated onto a solid substrate, and differentiated in the presence of select factors and morphogens. After enrichment for cells with the appropriate phenotype, the cells are allowed to cluster into cardiac bodies��, which are remarkably homogeneous and suitable for the treatment of heart disease.

대표
청구항
▼

The invention claimed is: 1. A direct differentiation method of obtaining cardiomyocyte lineage cells from primate pluripotent stem (pPS) cells, comprising in the following order: a) obtaining undifferentiated pPS cells; b) plating the undifferentiated pPS cells without forming embryoid bodies directly onto a solid surface comprising a substrate to which cardiomyocyte lineage cells adhere without forming embryoid bodies; c) permitting the plated pPS cells to adhere onto the substrate; d) culturing the adhered cells in the absence of serum or feeder cell...

이 특허에 인용된 특허 (11)

  1. Xiao, Yong-Fu; Morgan, James P., Cardiac function comprising implantation of embryonic stem cell in which differentiation has been initiated.
  2. Mark F. Pittenger ; Stephen L. Gordon ; Alastair Morgan Mackay, Cardiac muscle regeneration using mesenchymal stem cells.
  3. Franz Wolfgang-Michael,DEX ; Wobus Anna M.,DEX, Embryonal cardiac muscle cells, their preparation and their use.
  4. Gearhart John D. ; Shamblott Michael Joseph, Human embryonic germ cell line and methods of use.
  5. Field Loren J., Myocardial grafts and cellular compositions.
  6. Field Loren J., Myocardial grafts and cellular compositions.
  7. Loren J. Field, Myocardial grafts and cellular compositions useful for same.
  8. Thomson James A., Primate embryonic stem cells.
  9. Cech Thomas R. ; Lingner Joachim,CHX ; Nakamura Toru ; Chapman Karen B. ; Morin Gregg B. ; Harley Calvin B. ; Andrews William H., Telomerase.
  10. Mickle Donald A. G.,CAX ; Li Ren-Ke,CAX ; Weisel Richard D.,CAX, Transplants for myocardial scars.
  11. Mickle Donald A. G.,CAXITX M5M 2X9 ; Li Ren-Ke,CAXITX M1V 1B4 ; Weisel Richard D.,CAXITX M4X 1S8, Transplants for myocardial scars and methods and cellular preparations.

이 특허를 인용한 특허 (30)

  1. Bohlen, Heribert; Jönsson, Kristina; Ehlich, Andreas; Schwengberg, Silke, Assay for drug discovery based on in vitro differentiated cells.
  2. Kamp, Timothy J.; Palecek, Sean; Zhang, Jianhua; Azarin, Samira; Lian, Xiaojun, Cardiac differentiation of human pluripotent stem cells under defined conditions using matrix overlay methods.
  3. Passier, Robert; Mummery, Christine Lindsay, Cardiomyocyte differentiation.
  4. Passier, Robert; Mummery, Christine Lindsay, Cardiomyocyte differentiation.
  5. Sun, Ning; Longaker, Michael T.; Robbins, Robert C.; Wu, Joseph; Lan, Feng; Lee, Andrew Stephen; Burridge, Paul W., Cardiomyocytes from induced pluripotent stem cells from patients and methods of use thereof.
  6. Terzic, Andre; Behfar, Atta, Compositions and methods for obtaining cells to treat heart tissue.
  7. Terzic, Andre; Behfar, Atta, Compositions and methods for obtaining cells to treat heart tissue.
  8. Xu, Chunhui, Compound screening using cardiomyocytes.
  9. Amit, Michal; Itskovitz-Eldor, Joseph, Culture media, cell cultures and methods of culturing pluripotent stem cells in an undifferentiated state.
  10. Lebkowski, Jane S.; Priest, Catherine A.; Okamura, Ross M., Differentiated pluripotent stem cell progeny depleted of extraneous phenotypes.
  11. Lebkowski, Jane S.; Priest, Catherine Ann; Okamura, Ross M., Differentiated pluripotent stem cell progeny depleted of extraneous phenotypes.
  12. Gold, Joseph D.; Hassanipour, Mohammad, Differentiation of primate pluripotent stem cells to cardiomyocyte-lineage cells.
  13. Xu, Chunhui, Differentiation protocol for making human cardiomyocytes.
  14. Gold, Joseph D.; Hassanipour, Mohammad; Collins, Lila R.; Xu, Chunhui, Direct differentiation method for making cardiomyocytes from human embryonic stem cells.
  15. Meng, Xingli; Schiffmann, Raphael; Shen, Jinsong, Establishment of patient- or person-specific cardiac myocyte cell lines from human induced pluripotent stem cells (iPSCs).
  16. Keller, Gordon; Yang, Lei; Kattman, Steven, Human cardiovascular progenitor cells.
  17. Amit, Michal; Itskovitz-Eldor, Joseph, Media for culturing stem cells.
  18. Amit, Michal; Itskovitz-Eldor, Joseph, Medium comprising transforming growth factor beta 1 and basic fibroblast growth factor.
  19. Amit, Michal; Itskovitz-Eldor, Joseph, Medium comprising transforming growth factor beta 1 and basic fibroblast growth factor.
  20. Amit, Michal; Itskovitz-Eldor, Joseph, Medium comprising transforming growth factor β1 and basic fibroblast growth factor.
  21. Terzic, Andre; Behfar, Atta, Methods and materials for providing cardiac cells.
  22. Amit, Michal; Itskovitz-Eldor, Joseph, Methods of expanding embryonic stem cells in a suspension culture.
  23. Amit, Michal; Itskovitz-Eldor, Joseph, Methods of expanding embryonic stem cells in a suspension culture.
  24. Bohlen, Heribert; Kolossov, Eugen; Kettenhofen, Ralf; Scholz, Melanie; Fink, Leo, Non-invasive, in vitro functional tissue assay systems.
  25. Xu, Chunhui; Gold, Joseph D., Process for making transplantable cardiomyocytes from human embryonic stem cells.
  26. Shogbon, Christopher Bankole; Zhou, Yue; Brandenberger, Ralph, Synthetic surfaces for culturing stem cell derived cardiomyocytes.
  27. Shogbon, Christopher Bankole; Zhou, Yue; Brandenberger, Ralph, Synthetic surfaces for culturing stem cell derived cardiomyocytes.
  28. Shogbon, Christopher Bankole; Zhou, Yue; Brandenberger, Ralph, Synthetic surfaces for culturing stem cell derived cardiomyocytes.
  29. Kolossov, Eugen; Hescheler, Jürgen; Bohlen, Heribert; Fleischmann, Bernd; Röll, Wilhelm; Ehlich, Andreas; Königsmann, Jessica, Tissue modeling in embryonic stem (ES) cell system.
  30. Terzic, Andre; Behfar, Atta, Treating cardiovascular tissue.

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