The present invention provides a common marmoset-derived glyceraldehyde-3-phosphate dehydrogenase gene and use thereof.
대표청구항▼
What is claimed is: 1. An isolated polynucleotide comprising a nucleotide sequence selected from the group consisting of: (1) the nucleotide sequence of SEQ ID NO: 1; (2) a nucleotide sequence encoding an amino acid sequence of a variant of SEQ ID NO: 2, wherein said variant is SEQ ID NO: 2 having
What is claimed is: 1. An isolated polynucleotide comprising a nucleotide sequence selected from the group consisting of: (1) the nucleotide sequence of SEQ ID NO: 1; (2) a nucleotide sequence encoding an amino acid sequence of a variant of SEQ ID NO: 2, wherein said variant is SEQ ID NO: 2 having glyceraldehyde 3-phosphate dehydrogenase activity and having one inserted, deleted or conservatively substituted amino acid; (3) a nucleotide sequence encoding the amino acid sequence of SEQ ID NO: 2; (4) a nucleotide sequence of DNA which has any of the nucleotide sequences as defined in (1) to (3), and is fully complementary to a mature mRNA containing a region from a 5' cap to a poly(A) sequence; and (5) a nucleotide sequence which is fully complementary to any of the nucleotide sequences as defined in (1) to (4). 2. A composition for detecting DNA or RNA, which comprises a polynucleotide of claim 1. 3. The composition according to claim 2, wherein said polynucleotide is immobilized on a carrier. 4. A method for measuring a difference in levels of expression of a desired gene in two or more kinds of test samples by measuring a difference in amounts of a transcript of the desired gene in said samples, which comprises: (1) measuring an amount of a transcript of a common marmoset-derived glyceraldehyde-3-phosphate dehydrogenase gene in two or more kinds of test samples using a polynucleotide of claim 1, (2) measuring an amount of a transcript of a desired gene in said test samples using a polynucleotide for detecting the desired gene, (3) normalizing the amount of the transcript of the desired gene measured in (2)in each test sample by using as an internal standard the amount of the transcript of the glyceraldehyde-3-phosphate dehydrogenase gene measured in (1) in said test sample, and (4) comparing the normalized amounts of the transcript of the desired gene in two or more kinds of test samples, thereby measuring a difference in the relative amounts of the transcript of the desired gene in the test samples. 5. The method according to claim 4, wherein said test samples are samples derived from common marmoset. 6. A method for measuring a difference in levels of expression of a desired gene in two or more kinds of test samples by measuring a difference in amounts of a transcript of the desired gene in said samples, which comprises: (1) measuring an amount of a transcript of a common marmoset-derived glyceraldehyde-3-phosphate dehydrogenase gene in two or more kinds of test samples using a polynucleotide of claim 1, (2) measuring an amount of a transcript of a desired gene in said test samples using a polynucleotide for detecting the desired gene, (3) calculating an inverse of the ratio of the amount of the transcript of the glyceraldehyde-3-phosphate dehydrogenase gene measured in (1) in each said test sample relative to the amount of the transcript of the glyceraldehyde-3-phosphate dehydrogenase gene measured in (1) a standard test sample that is to be used as a standard, (4) a fourth step of normalizing the amounts of the transcript of the desired gene measured in (2) in the test samples by multiplying the amount of the transcript of the desired gene measured in (2) in each test sample with the inverse of the ratio calculated in (3) for said test sample, and (5) comparing the normalized amounts of the transcript of the desired gene in two or more kinds of test samples, thereby measuring a difference in the relative amounts of the transcript of the desired gene in the test samples. 7. The method according to claim 6, wherein said test samples are samples derived from common marmoset. 8. The method according to claim 6, wherein the method of measuring an amount of a transcript of a gene in (1) and/or (2) is performed by either a DNA array method or a quantitative reverse transcriptase-polymerase chain reaction method. 9. A method for measuring a difference in levels of expression of a desired gene in two or more kinds of test samples by measuring a difference in amounts of a transcript of the desired gene in said samples, which comprises: (1) measuring an amount of a transcript of a common marmoset-derived glyceraldehyde-3-phosphate dehydrogenase gene in two or more kinds of test samples using a polynucleotide of claim 1, (2) measuring an amount of a transcript of a desired gene in said test samples using a polynucleotide for detecting the desired gene, (3) normalizing the amount of the transcript of the desired gene measured in (2) in the test samples by calculating the ratio of the amount of the transcript of the desired gene measured in (2) in each test sample relative to the amount of the transcript of the glyceraldehyde-3-phosphate dehydrogenase gene measured in (1) said test sample, and (4) comparing the normalized amounts of the transcript of the desired gene in two or more kinds of test samples, thereby measuring a difference in the relative amounts of the transcript of the desired gene in the test samples. 10. The method according to claim 9, wherein said test samples are samples derived from common marmoset. 11. The method according to claim 9, wherein the method of measuring an amount of a transcript of a gene is (1) and/or (2) is performed by either a DNA array method or a quantitative reverse transcriptase-polymerase chain reaction method. 12. An isolated polynucleotide consisting of a nucleotide sequence selected from the group consisting of: (1) the nucleotide sequence of SEQ ID NO:4 or 10; (2) the nucleotide sequence represented by nucleotide numbers 622 to 1221 of the nucleotide sequence of SEQ ID NO: 1; (3) a nucleotide sequence selected from the group consisting of: a consecutive nucleotide sequence of 60 nucleotides in length, which begins at any nucleotide of nucleotide number 805 to 806, 853 to 858, or 906 to 943 of the nucleotide sequence of SEQ ID NO: 1, and terminates at any nucleotide of nucleotide number 864 to 865, 912 to 917, or 965 to 1002 of the nucleotide sequence of SEQ ID NO: 1, respectively; a consecutive nucleotide sequence of 50 nucleotides in length, which begins at any nucleotide of nucleotide number 613, 643, 807 to 847, 902 to 927, 1005, 1039, 1057 to 1058, 1070 to 1086, or 1140 to 1160 of the nucleotide sequence of SEQ ID NO: 1, and terminates at any nucleotide of nucleotide number 662, 692, 856 to 896, 951 to 976, 1054, 1088, 1106 to 1107, 1119 to 1135 or 1189 to 1209 of the nucleotide sequence of SEQ ID NO: 1, respectively; a consecutive nucleotide sequence of 35 nucleotides in length, which begins at any nucleotide of nucleotide number 816 to 862, 902 to 942, 967 to 980, 1018, 1028 to 1042, 1070 to 1101, or 1150 to 1175 of the nucleotide sequence of SEQ ID NO: 1, and terminates at any nucleotide of nucleotide number 850 to 896, 936 to 976, 1001 to 1014, 1052, 1062 to 1076, 1104 to 1135, or 1184 to 1209 of the nucleotide sequence of SEQ ID NO: 1, respectively; a consecutive nucleotide sequence of 30 nucleotides in length, which begins at any nucleotide of nucleotide number 823 to 867, 902 to 945, 971 to 985, 1021 to 1037, 1070 to 1106, or 1152 to 1180 of the nucleotide sequence of SEQ ID NO: 1, and terminates at any nucleotide of nucleotide number 852 to 896, 931 to 974, 1000 to 1014, 1050 to 1066, 1099 to 1135, or 1181 to 1209 of the nucleotide sequence of SEQ ID NO: 1, respectively; and a consecutive nucleotide sequence of 20 nucleotides in length, which begins at any nucleotide of nucleotide number 836, 837, 859, 907, 908, 910, 921, 993, 994, 1004, 1032 or 1082 of the nucleotide sequence of SEQ ID NO: 1, and terminates at any nucleotide of nucleotide number 855, 856, 878, 926, 927, 929, 940, 1012, 1013, 1023, 1051 or 1101 of the nucleotide sequence of SEQ ID NO: 1, respectively; and (4) a nucleotide sequence which is fully complementary to any of the nucleotide sequences as defined in (1) to (3). 13. A composition for detecting DNA or RNA, which comprises a polynucleotide of claim 12. 14. The composition according to claim 13, wherein said polynucleotide is immobilized on a carrier. 15. A method for measuring a difference in levels of expression of a desired gene in two or more kinds of test samples by measuring a difference in amounts of a transcript of the desired gene in said samples, which comprises: (1) measuring an amount of a transcript of a common marmoset-derived glyceraldehyde-3-phosphate dehydrogenase gene in two or more kinds of test samples using a polynucleotide of claim 12, (2) measuring an amount of a transcript of a desired gene in said test samples using a polynucleotide for detecting the desired gene, (3) normalizing the amount of the transcript of the desired gene measured in (2) in each test sample by using as an internal standard the amount of the transcript of the glyceraldehyde-3-phosphate dehydrogenase gene measured in (1) in said test sample, and (4) comparing the normalized amounts of the transcript of the desired gene in two or more kinds of test samples, thereby measuring a difference in the relative amounts of the transcript of the desired gene in the test sample. 16. The method according to claim 15, wherein said test samples are samples derived from common marmoset. 17. A method for measuring a difference in levels of expression of a desired gene in two or more kinds of test samples by measuring a difference in amounts of a transcript of the desired gene in said samples, which comprises: (1) measuring an amount of a transcript of a common marmoset-derived glyceraldehyde-3-phosphate dehydrogenase gene in two or more kinds of test samples using a polynucleotide of claim 12, (2) measuring an amount of a transcript of a desired gene in said test samples using a polynucleotide for detecting the desired gene, (3) calculating an inverse of the ratio of the amount of the transcript of the glyceraldehyde-3-phosphate dehydrogenase gene measured in (1) in each said test sample relative to the amount of the transcript of the glyceraldehyde-3-phosphate dehydrogenase gene measured in (1) a standard test sample that is to be used as a standard, (4) normalizing the amounts of the transcript of the desired gene measured in (2) in the test samples by multiplying the amount of the transcript of the desired gene measured in (2) in each test sample with the inverse of the ratio calculated in (3) for said test sample, and (5) comparing the normalized amounts of the transcript of the desired gene in two or more kinds of test samples, thereby measuring a difference in the relative amounts of the transcript of the desired gene in test samples. 18. The method according to claim 17, wherein said test samples are samples derived from common marmoset. 19. The method according to claim 17, wherein the method of measuring an amount of a transcript of a gene in (1) and/or (2) is performed by either a DNA array method or a quantitative reverse transcriptase-polymerase chain reaction method. 20. A method for measuring a difference in levels of expression of a desired gene in two or more kinds of test samples by measuring a difference in amounts of a transcript of the desired gene in said samples, which comprises: (1) measuring an amount of a transcript of a common marmoset-derived glyceraldehyde-3-phosphate dehydrogenase gene in two or more kinds of test samples using a polynucleotide of claim 12, (2) measuring an amount of a transcript of a desired gene in said test samples using a polynucleotide for detecting the desired gene, (3) normalizing the amount of the transcript of the desired gene measured in (2) in the test samples by calculating the ratio of the amount of the transcript of the desired gene measured in (2) in each test sample relative to the amount of the transcript of the glyceraldehyde-3-phosphate dehydrogenase gene measured in (1) in said test sample, and (4) comparing the normalized amounts of the transcript of the desired gene in two or more kinds of test samples, thereby measuring a difference in the relative amounts of the transcript of the desired gene in the test samples. 21. The method according to claim 20, wherein said test samples are samples derived from common marmoset. 22. The method according to claim 20, wherein the method of measuring an amount of a transcript of a gene in (1) and/or (2) is performed by either a DNA array method or a quantitive reverse transcriptase-polymerase chain reaction method.
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