Disclosed are compounds capable of facilitating transport of biologically active agents or substances into cells having the general structure: wherein Q is selected from the group consisting of N, O and S; L is any bivalent organic radical capable of linking each Q, such as C, CH, (CH2)I, or {(C
Disclosed are compounds capable of facilitating transport of biologically active agents or substances into cells having the general structure: wherein Q is selected from the group consisting of N, O and S; L is any bivalent organic radical capable of linking each Q, such as C, CH, (CH2)I, or {(CH2)i-Y--(CH2)j}k, wherein Y is selected from the group consisting of CH2, an ether, a polyether, an amide, a polyamide, an ester, a sulfide, a urea, a thiourea, a guanidyl, a carbamoyl, a carbonate, a phosphate, a sulfate, a sulfoxide, an imine, a carbonyl, and a secondary amino group and wherein Y is optionally substituted by--X1-L'-X2-Z or-Z; R1-R6, independently of one another, are selected from the group consisting of H,--(CH2)p-D-Z, an alkyl, an alkenyl, an aryl, and an alkyl or alkyl ether optionally substituted by one or more of an alcohol, an aminoalcohol, an amine, an amide, an ether, a polyether, a polyamide, an ester, a mercaptan, an alkylthio, a urea, a thiourea, a guanidyl, or a carbamoyl group, and wherein at least one of R1, R3, R4 and R6 is a straight chain or branched, cyclic, alkyl, alkenyl, alkynyl or aryl group; and anyone of R1, R3, R4 and/or R6 may optionally be covalently linked with each other, with Y or with L when L is C or CH to form a cyclic moiety; Z is selected from the group consisting of amine, spermiyl, carboxyspermiyl, guanidyl, spermidinyl, putricinyl, diaminoalkyl, pyridyl, piperidinyl, pyrrolidinyl, polyamine, amino acid, peptide, and protein; X1 and X2, independently of one another, are selected from the group consisting of NH, O, S, alkylene, and arylene; L' is selected from the group consisting of alkylene, alkenylene, alkynylene, arylene, alkylene ether, and polyether; D is Q or a bond; A1 and A2, independently of one another, are selected from the group consisting of CH2O, CH2S, CH2NH, C(O), C{NH), C(S) and (CH2)t; X is a physiologically acceptable anion; m, n, r, s, u, v, w and y are 0 or 1, with the proviso that when both m and n are 0 at least one of r, s, u and y is other than 0; i, j, k, l, p and are integers from 0 to about 100; q is an integer from 1 to about 1000; and a is the number of positive charge divided by the valence of the anion.
대표청구항▼
We claim: 1. A compound having the formula: or a polycation thereof wherein R4 is a straight-chain, branched or cyclic, alkyl or alkenyl group having 8 to 24 carbon atoms, R5 is an alkyl group substituted with one or more amines and one or more alcohols, and R7 is H or a carbohydrate. 2. The c
We claim: 1. A compound having the formula: or a polycation thereof wherein R4 is a straight-chain, branched or cyclic, alkyl or alkenyl group having 8 to 24 carbon atoms, R5 is an alkyl group substituted with one or more amines and one or more alcohols, and R7 is H or a carbohydrate. 2. The compound of claim 1, wherein R5 is an alkyl group substituted with one amine and one or more alcohols. 3. The compound of claim 2, wherein the amine substituent of the R5 alkyl group comprises an aminoethanol, an aminopropanol or an aminobutanol group. 4. The compound of claim 3, wherein the amine substituent of the R5 alkyl group has the structure: wherein n is an integer from 7-23. 5. The compound of claim 1, wherein R5 is an alkyl group having 3-30 carbon atoms which is substituted by one amine and one or more alcohols. 6. The compound of claim 5, wherein the amine substituent of the R5 alkyl group comprises an aminoethanol, an aminopropanol or an aminobutanol group. 7. The compound of claim 6, wherein the amine substituent of the R5 alkyl group has the structure: wherein n is an integer from 7-23. 8. The compound of claim 5, wherein R5 is a propyl or a butyl group which is substituted by one amine and one or more alcohols. 9. The compound of claim 8, wherein the amine substituent of the R5 alkyl group comprises an aminoethanol, an aminopropanol or an aminobutanol group. 10. The compound of claim 9, wherein the amine substituent of the R5 alkyl group has the structure: wherein n is an integer from 7-23. 11. The compound of claim 1, wherein R7 is H. 12. The compound of claim 11, wherein R4 is a straight-chain alkyl group having 8 to 24 carbon atoms. 13. The compound of claim 12, wherein R5 is an alkyl group having 3-30 carbon atoms which is substituted with one amine and one or more alcohols. 14. The compound of claim 13, wherein the amine substituent of the R5 alkyl group comprises an aminoethanol, an aminopropanol or an aminobutanol group. 15. The compound of claim 14, wherein the amine substituent of the R5 alkyl group has the structure: wherein n is an integer from 7-23. 16. The compound of claim 13, wherein R5 is a propyl or a butyl group which is substituted by one amine and one or more alcohols. 17. The compound of claim 16, wherein the amine substituent of the R5 alkyl group comprises an aminoethanol, an aminopropanol or an aminobutanol group. 18. The compound of claim 17, wherein the amine substituent of the R5 alkyl group has the structure: wherein n is an integer from 7-23. 19. The compound of claim 1, wherein R4 is a straight-chain alkyl group having 8 to 24 carbon atoms, R5 is a butyl group substituted with one amine and one or more alcohols, and R7 is H. 20. The compound of claim 19, wherein the amine substituent of the R5 alkyl group comprises an aminoethanol, an aminopropanol or an aminobutanol group. 21. The compound of claim 20, wherein the amine substituent of the R5 alkyl group has the structure: wherein n is an integer from 7-23. 22. The compound of claim 1, wherein R4 is a straight-chain alkyl group having 8 to 24 carbon atoms, R5 is a propyl group substituted with one amine and one alcohol, and R7 is H. 23. The compound of claim 22, wherein the amine substituent of the R5 alkyl group comprises an aminoethanol, an aminopropanol or an aminobutanol group. 24. The compound of claim 23, wherein the amine substituent of the R5 alkyl group has the structure: wherein n is an integer from 7-23. 25. The compound of claim 19, which is a polycation. 26. The compound of claim 1, which is a polycation. 27. A composition comprising one or more cationic compounds of claim 1 solubilized in aqueous medium. 28. The composition of claim 27, further comprising a neutral lipid. 29. The composition of claim 28, wherein the neutral lipid is DOPE, DOPC or cholesterol. 30. The composition of claim 28, wherein the one or more compounds and the neutral lipid are formed into liposomes. 31. The composition of claim 27, further comprising a transfection enhancer. 32. A composition for transfecting a cell which comprises one or more compounds of claim 1 and one or more nucleic acids. 33. The composition of claim 32, wherein the nucleic acid is RNA. 34. The composition of claim 32, further comprising a neutral lipid. 35. The composition of claim 34, wherein the one or more compounds and the neutral lipid are formed into liposomes. 36. The composition of claim 32, further comprising a transfection enhancer. 37. A lipid aggregate made by mixing one or more compounds of claim 1 with a nucleic acid for at least 15 minutes to form a complex. 38. The lipid aggregate of claim 37, further comprising a neutral lipid. 39. The lipid aggregate of claim 38, wherein the neutral lipid is DOPE, DOPC or cholesterol. 40. The lipid aggregate of claim 37, wherein the lipid aggregate is 50 to 200 nm in diameter. 41. The lipid aggregate of claim 37, wherein the lipid aggregate is formed using extrusion, freeze-thaw, or sonication. 42. A kit comprising one or more container means and optionally further comprising one or more additional components selected from the group consisting of a cell, cells, a cell culture media, a nucleic acid and instructions for transfecting a cell or cells wherein said container means comprises one or more cationic compounds of claim 1. 43. The kit of claim 42, wherein the one or more cationic compounds are solubilized in aqueous medium. 44. The kit of claim 42, wherein the one or more cationic compounds are combined with one or more neutral lipids. 45. The kit of claim 44, wherein the neutral lipids are selected from DOPE, DOPC and cholesterol. 46. The kit of claim 42, wherein the one or more cationic compounds are formed into liposomes. 47. A method for introducing a polyanion into a cell or cells, wherein the method comprises the steps of: (a) forming a liposome from one or more compounds having the formula: or a polycation thereof wherein R4 is a straight-chain, branched or cyclic, alkyl or alkenyl group having 8 to 24 carbon atoms, R5 is an alkyl group substituted with one or more amines and one or more alcohols, and R7 is H or a carbohydrate; (b) contacting the liposome with the polyanion to form a positively-charged polyanion-liposome complex; and (c) incubating the complex with a cell or cells to thereby introduce the polyanion into the cell or cells. 48. The method of claim 47, wherein R5 is an alkyl group having 3-30 carbon atoms which is substituted with one amine and one or more alcohols. 49. The method of claim 48, wherein the amine substituent of the R5 alkyl group comprises an aminoethanol, an aminopropanol or an aminobutanol group. 50. The method of claim 49, wherein the amine substituent of the R5 alkyl group has the structure: wherein n is an integer from 7-23. 51. The method of claim 47, wherein R7 is H. 52. The method of claim 47, wherein the one or more compounds is a polycation. 53. The method of claim 47, wherein the polyanion is a nucleic acid. 54. The method of claim 53, wherein the nucleic acid is RNA. 55. The method of claim 53, wherein the nucleic acid inhibits expression of nucleic acids in the cell or cells. 56. The method of claim 47, wherein the cell or cells are selected from primary cells, tumor cells or cells of immortalized cell lines. 57. The method of claim 47, wherein the cell or cells are selected from epithelial, fibroblastic, neuronal, or hematopoietic cells. 58. The method of claim 47, wherein the cell or cells are those of an animal, fish, insect, fungus or plant. 59. The method of claim 47, wherein the cell or cells are CHO-K1, COS-7, or HEK293 cells. 60. The method of claim 47, wherein the liposome is formed by combining the one or more cationic compounds with a neutral lipid. 61. The method of claim 60, wherein the neutral lipid is selected from DOPE, DOPC or cholesterol.
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이 특허에 인용된 특허 (207)
Cotten Matthew (Vienna ATX) Wagner Ernst (Langenzersdorf ATX), Adenovirus for delivering foreign DNA into higher eukaryotic cells.
Wolff Jon A. (1122 University Bay Dr. Madison WI 53705) Budker Vladimir (204 N. Segoe Rd. #513 Madison WI 53705) Gurevich Vladimir (2113 E. Johnson St. Madison WI 53704), Amphipathic, micellar delivery systems for biologically active polyions.
Merianos John J. (Middletown NJ), Antimicrobial, low toxicity, non-irritating composition comprising a blend of bis-quaternary ammonium compounds coprecip.
Dean Nicholas M. ; McKay Robert ; Miraglia Loren ; Baker Brenda, Antisense oligonucleotide compositions and methods for the inibition of c-Jun and c-Fos.
Crooke Stanley T. (Carlsbad CA) Mirabelli Christopher K. (Encinitas CA) Ecker David J. (Carlsbad CA) Cowsert Lex M. (Carlsbad CA), Antisense oligonucleotides directed to papillomavirus.
Tomalia Donald A. ; Baker James R. ; Cheng Roberta C. ; Bielinska Anna U. ; Fazio Michael J. ; Hedstrand David M. ; Johnson Jennifer A. ; Kaplan ; deceased Donald A. ; Klakamp Scott L. ; Kruper ; Jr., Bioactive and/or targeted dendrimer conjugates.
Ravikumar Vasulinga (Carlsbad CA) Andrade Mark (Carlsbad CA) Mulvey Dennis (Conroe TX) Cole Douglas L. (San Diego CA), Carbocation scavenging during oligonucleotide synthesis.
Scheule Ronald K. ; Bagley Rebecca G. ; Eastman Simon J. ; Cheng Seng H. ; Marshall John ; Yew Nelson S. ; Harris David J. ; Lee Edward R. ; Siegel Craig S. ; Chang Chau-Dung ; Hubbard S. Catherine, Cationic amphiphile/DNA complexes.
Rajkumar Banerjee IN; Prasanta Kumar Das IN; Gollapudi Venkata Srilakshmi IN; Nalam Madhusudhana Rao IN; Arabinda Chaudhuri IN, Cationic amphiphiles containing N-hydroxyalkyl group for intracellular delivery of biologically active molecules.
Harris David J. ; Lee Edward R. ; Siegel Craig S. ; Cheng Seng H. ; Eastman Simon J. ; Marshall John ; Scheule Ronald K., Cationic amphiphiles containing dialkylamine lipophilic groups for intracellular delivery of therapeutic molecules.
Keynes Mikaela N. ; Siegel Craig S. ; Lee Edward R. ; Harris David J., Cationic amphiphiles containing linkers derived from neutral or positively charged amino acids.
Lee Edward R. ; Harris David J. ; Siegel Craig S. ; Cheng Seng H. ; Eastman Simon J. ; Marshall John ; Scheule Ronald K., Cationic amphiphiles containing spermine or spermidine cationic group for intracellular delivery of therapeutic molecul.
Harris David J. (Lexington MA) Lee Edward R. (Quincy MA) Siegel Craig S. (Woburn MA) Cheng Seng H. (Wellesley MA) Eastman Simon J. (Marlboro MA) Marshall John (Milford MA), Cationic amphiphiles for intracellular delivery of therapeutic molecules.
Gebeyehu Gulilat (Silver Spring MD) Jessee Joel A. (Mt. Airey MD) Ciccarone Valentina C. (Gaithersburg MD) Hawley-Nelson Pamela (Silver Spring MD) Chytil Anna (Nashville TN), Cationic lipids.
Felgner Philip L. (Rancho Santa Fe CA) Kumar Raj (San Diego CA) Basava Channa (San Diego CA) Border Richard C. (Poway CA) Hwang-Felgner Jiin-Yu (Rancho Santa Fe CA), Cationic lipids for intracellular delivery of biologically active molecules.
Felgner, Philip L.; Kumar, Raj; Basava, Channa; Border, Richard C.; Hwang-Felgner, Jiin-Yu, Cationic lipids for intracellular delivery of biologically active molecules.
Nantz Michael H. (Department of Chemistry ; Univ. or California Davis CA 95616) Bennett Michael J. (4849 El Cemonte Ave. ; #163 Davis CA 95616) Malone Robert W. (1016 Bienville St. Davis CA 95616), Cationic transport reagents.
Curiel David T. (Chapel Hill NC) Birnstiel Max L. (Vienna ATX) Cotten Matthew (Vienna ATX) Wagner Ernst (Langenzersdorf ATX) Zatloukal Kurt (Vienna ATX) Plank Christian (Vienna ATX) Oberhauser Berndt, Composition for introducing nucleic acid complexes into higher eucaryotic cells.
Fasbender Allen J. ; Welsh Michael J. ; Siegel Craig S. ; Lee Edward R. ; Chang Chau-Dung ; Marshall John ; Cheng Seng H. ; Harris David J. ; Eastman Simon J. ; Hubbard Shirley C. ; Lane Mathieu B. ;, Compositions comprising cationic amphiphiles and co-lipids for intracellular delivery of therapeutic molecules.
Curiel David T. (Chapel Hill NC) Hu Ping-chuan (Chapel Hill NC) Birnstiel Max L. (Vienna ATX) Cotten Matthew (Vienna ATX) Wagner Ernst (Langenzersdorf ATX), Conjugates for introducing nucleic acid into higher eucaryotic cells.
Felgner Philip L. (Rancho Santa Fe CA) Wolff Jon A. (Madison WI) Rhodes Gary H. (Leucadia CA) Malone Robert W. (Chicago IL) Carson Dennis A. (Del Mar CA), Delivery of exogenous DNA sequences in a mammal.
Tomalia Donald A. (Midland MI) Wilson Larry R. (Beaverton MI) Hedstrand David M. (Midland MI) Tomlinson Ian A. (Midland MI) Fazio Michael J. (Midland MI) Kruper ; Jr. William J. (Sanford MI) Kaplan D, Dense star polymer conjugates.
Vary Calvin P. H. (Califon NJ) Diamond Steven E. (Springfield NJ) Wolfman Neil M. (Brookline MA), Diagnostic reagent, kit and method employing polynucleotide displacement, separation, enzymatic cleavage and adenosine p.
Piwnica-Worms David R. (Wesseslley MA) Kronauge James F. (Brookline MA), Enhancement of cellular accumulation of lipophilic cationic organometallic compounds by reduction of intramembrane poten.
Shih PoJen (Columbia MD) Hawley-Nelson Pamela (Silver Spring MD) Jessee Joel A. (Mt. Airy MD), Enhancement of lipid cationic transfections in the presence of serum.
Nantz Michael H. ; Bennett Michael J. ; Balasubramaniam Rajiv P. ; Aberle Alfred M. ; Malone Robert W., Formulations and methods for generating active cytofectin: polynucleotide transfection complexes.
Felgner Philip L. (Rancho Santa Fe CA) Wolff Jon Asher (Madison WI) Rhodes Gary H. (Leucadia CA) Malone Robert Wallace (Davis CA) Carson Dennis A. (Del Mar CA), Generation of antibodies through lipid mediated DNA delivery.
Flotte Terence R. (Glen Burnie MD) Carter Barrie J. (Seattle WA) Guggino William B. (Baltimore MD) Solow Rikki (Gaithersburg MD), Generation of high titers of recombinant AAV vectors.
Piwnica-Worms David R. (Wellesley MA) Kronauge James F. (Brookline MA), In vivo enhancement of cellular accumulation of lipophilic cationic organometallic compounds by reduction of intramembra.
Felgner Philip L. (Rancho Santa Fe CA) Wolff Jon A. (Madison WI) Rhodes Gary H. (Leucadia CA) Malone Robert W. (Chicago IL) Carson Dennis A. (Del Mar CA), Induction of a protective immune response in a mammal by injecting a DNA sequence.
Felgner Philip L. ; Wolff Jon Asher ; Rhodes Gary H. ; Malone Robert Wallace ; Carson Dennis A., Induction of a protective immune response in a mammal by injecting a DNA sequence.
Cohen Jack S. (Bethesda MD) Neckers Len (Bethesda MD) Stein Cy (Gaithersburg MD) Loke She L. (Wheaton MD) Shinozuka Kazuo (Kazo JPX), Inhibitors for replication of retroviruses and for the expression of oncogene products.
Cohen Jack S. (Bethesda MD) Neckers Len (Bethesda MD) Stein Cy (Gaithersburg MD) Loke She L. (Wheaton MD) Shinozuka Kazuo (Kazo JPX), Inhibitors for replication of retroviruses and for the expression of oncogene products.
Cohen Jack S. (Bethesda MD) Neckers Len (Bethesda MD) Stein Cy (Gaithersburg MD) Loke Shee L. (Wheaton MD) Shinozuka Kazuo (Kazo JPX), Inhibitors for replication of retroviruses and for the expression of oncogene products.
Rainer Bischoff SE; Abdesslame Nazih FR; Yves Cordier FR, Lipid compounds and compositions containing same used for the transfer of at least an active substance, in particular a polynucleotide, in a target cell and therapeutic use.
Epand Richard M. (Hamilton CAX) Bottega Remo (Ancaster,both of PA CAX) Huang Leaf (Upper St. Claim PA), Method for delivering nucleic acids into cells.
Jack J. Hawiger ; Daniel Robinson ; Ruth Ann Veach ; Xue Yan Liu ; Danya Liu ; Sheila Timmons ; Robert D. Collins, Method for importing biologically active molecules into cells.
Ludwig Linda B. (East Aurora NY), Method for preselecting recombinant clones containing a specific nucleic acid sequence and subsequent transformation wit.
Papahadjopoulos Demetrios P. (78 Heathwood Williamsville NY 14221) Szoka ; Jr. Francis C. (375 Leroy Ave. Buffalo NY 14214), Method of encapsulating biologically active materials in lipid vesicles.
Li Wu-Bo (N. Potomac MD) Gruber Christian E. (Frederick MD) Jessee Joel A. (Mt. Airy MD) Lin Jhy-Jhu (Gaithersburg MD), Method of nucleic acid sequence selection.
Eppstein Deborah A. (Menlo Park CA) Felgner Philip L. (Los Altos CA) Gadek Thomas R. (Oakland CA) Jones Gordon H. (Cupertino CA) Roman Richard B. (Fairhope AL), N-(1,(1-1)-dialkyloxy)-and N-(1,(1-1)-dialkenyloxy alk-1-yl-N-N,N-tetrasubstituted ammonium lipids and uses therefor.
Eppstein Deborah A. (Menlo Park CA) Felgner Philip L. (Los Altos CA) Gadek Thomas R. (Oakland CA) Jones Gordon H. (Cupertino CA) Roman Richard B. (Fairhope AL), N-(www
상세보기
Eppstein Deborah A. (Menlo Park CA) Felgner Philip L. (Los Altos CA) Gadek Thomas R. (Oakland CA) Jones Gordon H. (Cupertino CA) Roman Richard B. (Fairhope AL), N-(www
상세보기
Eppstein Deborah A. (Menlo Park CA) Felgner Philip L. (Los Altos CA) Gadek Thomas R. (Oakland CA) Jones Gordon H. (Cupertino CA) Roman Richard B. (Fairhope AL), N-www
상세보기
Deborah A. Eppstein ; Philip L. Felgner ; Thomas R. Gadek ; Gordon H. Jones ; Richard B. Roman, N-[1, (1-1) -dialkyloxy] - and N- [1, (1-1) -dialkenyloxy]- alk-1-yl-N,N,N-tetrasubstituted ammonium lipids and uses therefor.
Eppstein Deborah A. (Menlo Park CA) Felgner Philip L. (Los Altos CA) Gadek Thomas R. (Oakland CA) Jones Gordon H. (Cupertino CA) Roman Richard B. (Fairhope AL), N-[1, (1-1)-dialkyloxy]-and N-[1, (1-1)-dialkenyloxy]-alk-1-yl-n,n,n-tetrasubstituted ammonium lipids and uses therefor.
Eppstein Deborah A. (Menlo Park CA) Felgner Philip L. (Los Altos CA) Gadek Thomas R. (Oakland CA) Jones Gordon H. (Cupertino CA) Roman Richard B. (Fairhope AL), N[www
상세보기
Tomalia Donald A. ; Hedstrand David M. ; Yin Rui, Non-crosslinked, polybranched polymers.
Draper Kenneth G. (Boulder CO) Crooke Stanley T. (Carlsbad CA) Mirabelli Christopher K. (Encinitas CA) Ecker David J. (Leucadia CA) Hanecak Ronnie C. (San Clemente CA) Anderson Kevin P. (Carlsbad CA), Oligonucleotide therapies for modulating the effects of herpes viruses.
Meyer ; Jr. Rich B. (Woodinville WA) Gall Alexander A. (Bothell WA) Reed Michael W. (Seattle WA), Peptide linkers for improved oligonucleotide delivery.
Hawley-Nelson Pamela ; Lan Jianqing ; Shih PoJen ; Jessee Joel A. ; Schifferli Kevin P. ; Gebeyehu Gulilat, Peptide-enhanced cationic lipid transfections.
Pamela Hawley-Nelson ; Jianqing Lan ; PoJen Shih ; Joel A. Jessee ; Kevin P. Schifferli ; Gulilat Gebeyehu ; Valentina C. Ciccarone ; Krista L. Evans, Peptide-enhanced transfections.
Kahne Daniel E. (Princeton NJ) Kahne Suzanne W. (Princeton NJ), Pharmaceutical compositions and methods of transporting compounds across biological membrane.
Nantz Michael H. ; Bennett Michael J. ; Balasubramaniam Rajiv P. ; Aberle Alfred M. ; Malone Robert W., Polyfunctional cationic cytofectins, formulations and methods for generating active cytofectin: polynucleotide transfec.
Haas Peter (Haan DEX) Blahak Johannes (Cologne DEX) Meiners Hans-Joachim (Leverkusen DEX) Mormann Werner (Leverkusen DEX), Polyurethanes made with linear polyamines.
Mullis Kary B. (La Jolla CA) Erlich Henry A. (Oakland CA) Arnheim Norman (Woodland Hills CA) Horn Glenn T. (Emeryville CA) Saiki Randall K. (Richmond CA) Scharf Stephen J. (Berkeley CA), Process for amplifying, detecting, and/or cloning nucleic acid sequences.
Mullis Kary B. (La Jolla CA) Erlich Henry A. (Oakland CA) Gelfand David H. (Oakland CA) Horn Glenn (Emeryville CA) Saiki Randall K. (Richmond CA), Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme.
Mullis Kary B. (Kensington CA) Erlich Henry A. (Oakland CA) Arnheim Norman (Woodland Hills CA) Horn Glenn T. (Emeryville CA) Saiki Randall K. (Richmond CA) Scharf Stephen J. (Berkeley CA), Process for amplifying, detecting, and/or-cloning nucleic acid sequences.
Yin Rui (Mount Pleasant MI) Tomalia Donald A. (Midland MI) Hedstrand David M. (Midland MI) Swanson Douglas R. (Midland MI), Process for producing hyper-comb-branched polymers.
Banerjee Rajkumar,INX ; Das Prasanta Kumar,INX ; Srilakshmi Gollapudi Venkata,INX ; Rao Nalam Madhusudhana,INX ; Chaudhuri Arabinda,INX, Process for synthesis of novel cationic amphiphiles containing N-hydroxyalkl group for intracellular delivery of biologically active molecules.
Banerjee, Rajkumar; Das, Prasanta Kumar; Srilakshmi, Gollapudi Venkata; Rao, Nalam Madhusudhana; Chaudhuri, Arabinda, Process for synthesis of novel cationic amphiphiles containing N-hydroxyalkyl group for intracellular delivery of biologically active molecules.
Wolff Jon A. ; Fritz Jeffery ; Budker Vladimir ; Hagstrom James, Process of transfecting a cell with a polynucleotide mixed with an amphipathic compound and a DNA-binding protein.
Wolff Jon A. ; Hagstrom James E. ; Budker Vladimir G. ; Fritz Jeffery, Process of transfecting a cell with a polynucleotide mixed with an amphipathic compound and a DNA-binding protein.
Harman Gary E. ; Broadway Roxanne M. ; Tronsmo Arne,NOX ; Lorito Matteo ; Hayes Christopher K. ; Di Pietro Antonio,CHX, Purified chitinases and use thereof.
Gelfand David H. (Oakland CA) Stoffel Susanne (El Cerrito CA) Lawyer Frances C. (Oakland CA) Saiki Randall K. (Richmond CA), Purified thermostable enzyme.
Gelfand David H. (Oakland CA) Stoffel Susanne (El Cerrito CA) Lawyer Frances C. (Oakland CA) Saiki Randall K. (Richmond CA), Purified thermostable enzyme.
Pastan Ira H. (Potomac MD) Trevor Prior (Bethesda MD) Fitzgerald David J. (Silver Spring MD) Debinski Waldemar (Gaithersburg MD) Siegall Clay (Silver Springs MD), Recombinant chimeric proteins deliverable across cellular membranes into cytosol of target cells.
Szoka ; Jr. Francis C. (45 Mendosa Ave. San Francisco CA 94116) Haensler Jean (117 ; Rue Principale 57540 Petite-Rosselle FRX), Self-assembling polynucleotide delivery system comprising dendrimer polycations.
Oberst Richard D. (Manhattan KS) Gwaltney Sharon M. (Manhattan KS) Hays Michael P. (Leonardville KS), Specific DNA primers and method to use same detect Eperythrozoon suis.
Tomalia Donald A. (Midland MI) Kaplan Donald A. (Cincinnati OH) Kruper ; Jr. William J. (Sanford MI) Cheng Roberta C. (Midland MI) Tomlinson Ian A. (Midland MI) Fazio Michael J. (Midland MI) Hedstran, Starburst conjugates.
Hedstrand David M. (Midland MI) Helmer Bradley J. (Midland MI) Tomalia Donald A. (Midland MI), Structured copolymers and their use as absorbents, gels and carriers of metal ions.
Barnes Wayne M. (223 Renaldo Dr. Chesterfield MO 63017), Thermostable DNA polymerase with enhanced thermostability and enhanced length and efficiency of primer extension.
Nabel Elizabeth G. (Ann Arbor MI) Nabel Gary J. (Ann Arbor MI), Treatment of diseases by site-specific instillation of cells or site-specific transformation of cells and kits therefor.
Yang, Zhiwei; Angrish, Parul; de Mollerat du Jeu, Xavier; Wiederholt, Kristin; Wu, Tao, Amine-containing transfection reagents and methods for making and using same.
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