The invention relates to materials and methods useful for controlling the unwanted spread of transgenic traits. The methods involve an apomictic plant containing a transgene for a desired trait and a transgene causing seed infertility. The methods also involve one or more transcription activators th
The invention relates to materials and methods useful for controlling the unwanted spread of transgenic traits. The methods involve an apomictic plant containing a transgene for a desired trait and a transgene causing seed infertility. The methods also involve one or more transcription activators that activates expression of both transgenes carried by the apomictic plant. The transcription activator(s) activates expression of both transgenes in the female. Seeds that are formed on such plants are infertile.
대표청구항▼
What is claimed is: 1. A method for making infertile seed, said method comprising: a) growing a plurality of apomictic plants, wherein said plants comprise first and second nucleic acids, said first nucleic acid comprising a first transcription activator recognition site and a first promoter, said
What is claimed is: 1. A method for making infertile seed, said method comprising: a) growing a plurality of apomictic plants, wherein said plants comprise first and second nucleic acids, said first nucleic acid comprising a first transcription activator recognition site and a first promoter, said first recognition site and said first promoter operably linked to a sequence to be transcribed, said second nucleic acid comprising a second transcription activator recognition site and a second promoter, said second recognition site and said second promoter operably linked to a coding sequence that results in seed infertility, wherein said coding sequence that results in seed infertility is SEQ ID NO: 70; and wherein said plants comprise at least one activator nucleic acid comprising at least one coding sequence for a transcription activator that binds to at least one of said recognition sites, each said at least one transcription activator coding sequence having a promoter operably linked thereto, and wherein seeds that form on said plants are infertile, wherein said first and second promoters and said promoter operably linked to said at least one transcription activator coding sequence are selected from a CaMV35S minimal promoter and a rice or maize ubiquitin minimal promoter; wherein said first and second transcription activator recognition sites comprise from 1 to 5 copies of a UASHap1 upstream activator sequence element; and wherein said transcription activator is a chimeric polypeptide comprising a yeast HAP1 DNA binding domain and a herpes simplex VP16 transcription activator domain. 2. The method of claim 1, wherein said plants are dicotyledonous plants. 3. The method of claim 1, wherein said plants are monocotyledonous plants. 4. The method of claim 1, wherein said sequence to be transcribed encodes a preselected polypeptide. 5. The method of claim 4, wherein expression of said preselected polypeptide confers herbicide resistance. 6. A method for making a polypeptide, said method comprising: a) growing a plurality of apomictic plants, each of said plants comprising: i) a first nucleic acid comprising a first transcription activator recognition site and a first promoter, said first recognition site and said first promoter operably linked to a nucleic acid encoding a preselected polypeptide; and ii) a second nucleic acid comprising a second transcription activator recognition site and a second promoter, said second recognition site and said second promoter operably linked to a sequence causing seed infertility, wherein said sequence causing seed infertility is SEQ ID NO: 70 and wherein said plants comprise at least one activator nucleic acid encoding at least one transcription activator that binds to at least one of said recognition sites, each said at least one transcription activator nucleic acid having a promoter operably linked thereto, wherein said first and second promoters and said promoter operably linked to said at least one transcription activator nucleic acid are selected from a CaMV35S minimal promoter and a rice or maize ubiquitin minimal promoters; wherein said first and second transcription activator recognition sites comprise from 1 to 5 copies of a UASHap1 upstream activator sequence element; and wherein said transcription activator is a chimeric polypeptide comprising a yeast HAP1 DNA binding domain and a herpes simplex VP16 transcription activator domain; and b) expressing said preselected polypeptide in said plants, wherein said plants have a statistically significant increase in said preselected polypeptide relative to plants that do not contain or express said first nucleic acid and wherein seeds that develop on said plants are infertile. 7. An article of manufacture comprising: a) a container; b) apomictic seeds within said container, said seeds comprising at least one first nucleic acid comprising: i) a first transcription activator recognition site and a first promoter, said first recognition site and said first promoter operably linked to a sequence to be transcribed; ii) a second transcription activator recognition site and a second promoter, said second recognition site and said second promoter operably linked to a sequence causing seed infertility wherein said sequence causing seed infertility is SEQ ID NO: 70; and iii) at least one activator nucleic acid encoding at least one transcription activator that binds to at least one of said recognition sites, each said at least one transcription activator nucleic acid having a promoter operably linked thereto, wherein plants grown from said seeds are infertile, wherein said first and second promoters and said promoter operably linked to said at least one transcription activator nucleic acid are selected from a CaMV35S minimal promoter and a rice or maize ubiquitin minimal promoter; wherein said first and second transcription activator recognition sites comprise from 1 to 5 copies of a UASHap1 upstream activator sequence element; and wherein said transcription activator is a chimeric polypeptide comprising a yeast HAP1 DNA binding domain and a herpes simplex VP16 transcription activator domain. 8. The method of claim 7, wherein said sequence to be transcribed encodes a preselected polypeptide. 9. The article of claim 7, wherein said seeds are dicotyledonous seeds. 10. The article of claim 7, wherein said seeds are monocotyledonous seeds. 11. An apomictic plant comprising: a) a first nucleic acid comprising a first transcription activator recognition site and a first promoter, said first recognition site and said first promoter operably linked to a sequence to be transcribed, b) a second nucleic acid comprising a second transcription activator recognition site and a second promoter, said second recognition site and said second promoter operably linked to a sequence causing seed infertility, wherein said sequence causing seed infertility is SEQ ID NO:70, and c) at least one activator nucleic acid comprising at least one coding sequence for a transcription activator that binds to at least one of said recognition sites, each said at least one transcription activator coding sequence having a promoter operably linked thereto, wherein seeds that develop on said plant are infertile, wherein said first and second promoters and said promoter operably linked to said at least one transcription activator coding sequence are selected from a CaMV35S minimal promoter and a rice or maize ubiquitin minimal promoter; wherein said first and second transcription activator recognition sites comprise from 1 to 5 copies of a UASHap1 upstream activator sequence element; and wherein said transcription activator is a chimeric polypeptide comprising a yeast HAP1 DNA binding domain and a herpes simplex VP16 transcription activator domain. 12. The plant of claim 11, wherein said plant is a dicotyledonous plant. 13. The plant of claim 11, wherein said plant is a monocotyledonous plant. 14. The plant of claim 11, wherein said sequence to be transcribed encodes a preselected polypeptide that confers herbicide resistance.
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Leemans Jan (Heusden BEX) Botterman Johan (Zwijnaarde BEX) De Block Marc (Gentbrugge BEX) Thompson Charles (Grand Lancy/Genege CHX) Mouva Rao (Genev CHX), Genetically engineered plant cells and plants exhibiting resistance to glutamine synthetase inhibitors, DNA fragments an.
Shah Dilip M. (Creve Coeur MO) Rogers Stephen G. (Chesterfield MO) Horsch Robert B. (both St. Louis MO) Fraley Robert T. (both St. Louis MO), Glyphosate-resistant plants.
Endege Wilson O. ; Steinmann Kathleen E. ; Astle Jon H. ; Burgess Christopher C. ; Carroll ; III Eddie ; Catino Theodore J. ; Dwivedi Poornima ; Ford Donna M. ; Lewis Marcia E. ; Molino Gary A. ; Mon, Human genes and expression products: II.
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Sanford John C. (Geneva) DeVit Michael J. (Geneva NY) Bruner Ronald F. (Sewell NJ) Johnston Stephen A. (Durham NC), Method and apparatus for introducing biological substances into living cells.
Hiatt Andrew C. ; Ma Julian K.-C.,GBX ; Lehner Thomas,GBX ; Mostov Keith E., Method for producing immunoglobulins containing protection proteins in plants and their use.
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Bedbrook John R. (Piedmont CA) Chaleff Roy S. (Pennington NJ) Falco Saverio C. (Arden DE) Mazur Barbara J. (Wilmington DE) Somerville Christopher R. (Okemon MI) Yadav Narendra S. (Wilmington DE), Nucleic acid fragment encoding herbicide resistant plant acetolactate synthase.
Fischer Robert L. ; Ohad Nir,ILX ; Kiyosue Tomohiro,JPX ; Yadegari Ramin ; Margossian Linda ; Harada John ; Goldberg Robert B., Nucleic acids that control endosperm development in plants.
Strauch Eckhard (Bielefeld DEX) Arnold Walter (Bielefeld DEX) Alijah Renate (Bielefeld DEX) Wohlleben Wolfgang (Bielefeld DEX) Phler Alfred (Bielefeld DEX) Eckes Peter (Kelkheim/Taunus DEX) Donn Gunt, Phosphinothricin-resistance gene, and its use.
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