Methods, compositions, and kits for the detection of bacteria in a sample
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
C12Q-001/00
C12N-011/14
C12N-011/00
C12M-001/00
출원번호
US-0149979
(2005-06-09)
등록번호
US-7491488
(2009-02-17)
발명자
/ 주소
Fleming,James E.
Somes,Jason Buck
출원인 / 주소
GenPrime, Inc.
대리인 / 주소
Seed IP Law Group PLLC
인용정보
피인용 횟수 :
0인용 특허 :
6
초록▼
The present invention includes compositions, kits, and methods useful for the detection of bacteria. These agents and methods are primarily directed to a method of detecting the presence of bacteria in a sample, involving incubating the sample with an agent that binds to bacteria, such as, e.g., an
The present invention includes compositions, kits, and methods useful for the detection of bacteria. These agents and methods are primarily directed to a method of detecting the presence of bacteria in a sample, involving incubating the sample with an agent that binds to bacteria, such as, e.g., an agent specific for peptidoglycan or a component thereof, and then detecting bound bacteria. The invention includes lateral-flow immunoassay methods and devices for assessing the total bacterial load in a liquid sample.
대표청구항▼
The invention claimed is: 1. A device for the detection of bacteria, comprising: (a) a self-contained carrier to which a liquid sample suspected of containing bacteria can be applied; (b) a first binding agent that is mobile in the carrier when in the moist state; (c) a second binding agent that is
The invention claimed is: 1. A device for the detection of bacteria, comprising: (a) a self-contained carrier to which a liquid sample suspected of containing bacteria can be applied; (b) a first binding agent that is mobile in the carrier when in the moist state; (c) a second binding agent that is immobilized in a first region of the carrier, wherein said second binding agent is an antibody, and wherein both of the binding agents bind peptidoglycan; and (d) a detectable labeled secondary agent that binds the first binding agent, wherein the secondary agent comprises a detectable label and the secondary agent is not an antibody. 2. The device of claim 1, wherein the carrier comprises the following components: (a) a liquid sample application wick; (b) a conjugate pad comprising the first binding agent; (c) a base pad comprising the second binding agent; (d) a sample pad comprising a blocking solution; and (e) an absorbent pad. 3. The device of claim 1, further comprising a control binding agent that is immobilized in a second region of the carrier. 4. The device of claim 1, wherein the first binding agent is labeled with biotin, and the labeled secondary agent comprises streptavidin. 5. The device of claim 4, wherein the streptavidin is conjugated to colloidal gold. 6. The device of claim 1, wherein the sample is a blood product. 7. The device of claim 1, wherein the first binding agent is not an antibody. 8. The device of claim 7, where the first binding agent is a peptidoglycan recognition protein (PGRP). 9. A device for the detection of bacteria, comprising: (a) a self-contained carrier to which a liquid sample suspected of containing bacteria can be applied; (b) a first binding agent that is mobile in the carrier when in the moist state, wherein the first binding agent is not an antibody; (c) a second binding agent that is immobilized in a first region of the carrier, wherein said second binding agent is an antibody, and wherein both of the binding agents bind peptidoglycan; and (d) a detectable labeled secondary agent that binds the first binding agent, wherein the secondary agent comprises a detectable label and the secondary agent is not an antibody. 10. The device of claim 9, where the first binding agent is a peptidoglycan recognition protein (PGRP).
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이 특허에 인용된 특허 (6)
Paul James Davis GB; Michael Evans Prior GB; Keith May GB, Analytical test device for imuno assays and methods of using same.
May Keith (Bedfordshire GB2) Prior Michael E. (Northamptonshire GB2) Richards Ian (Bedford GB2), Capillary immunoassay and device therefor comprising mobilizable particulate labelled reagents.
Shockman Gerald D. (Philadelphia PA) Jackson Dianne E. (Brookline MA) Wong William (Wilmington DE), Monoclonal antibodies to peptidoglycan and methods of preparing same.
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