Methods for increasing stearate content in soybean oil
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
A01H-005/00
C12N-015/82
출원번호
US-0305021
(2005-12-19)
등록번호
US-7504563
(2009-03-17)
발명자
/ 주소
Kridl,Jean
출원인 / 주소
Monsanto Technology LLC
대리인 / 주소
Li,Chunping
인용정보
피인용 횟수 :
5인용 특허 :
19
초록▼
This invention relates to a method for increasing stearate as a component of total triglycerides found in soybean seed. The method generally comprises growing a soybean plant having integrated into its genome a DNA construct comprising, in the 5' to 3' direction of transcription, a promoter function
This invention relates to a method for increasing stearate as a component of total triglycerides found in soybean seed. The method generally comprises growing a soybean plant having integrated into its genome a DNA construct comprising, in the 5' to 3' direction of transcription, a promoter functional in a soybean plant seed cell, a DNA sequence encoding an acyl-ACP thioesterase protein having substantial activity on C18:0 acyl-ACP substrates, and a transcription termination region functional in a plant cell. The present invention also provides a soybean seed with about 33 weight percent or greater stearate as a component of total fatty acids found in seed triglycerides.
대표청구항▼
What is claimed is: 1. A soybean seed comprising an introduced nucleic acid sequence having in the 5' to 3' direction of transcription: a promoter that is functional in a soybean seed cell and a DNA sequence encoding a plant FatA thioesterase, wherein said plant FatA thioesterase has the sequence o
What is claimed is: 1. A soybean seed comprising an introduced nucleic acid sequence having in the 5' to 3' direction of transcription: a promoter that is functional in a soybean seed cell and a DNA sequence encoding a plant FatA thioesterase, wherein said plant FatA thioesterase has the sequence of Garcinia mangostana (Garm) FatA1 (SEQ ID NO: 2), and said plant FatA thioesterase has a high relative activity for 18:0-acyl-acyl-carrier protein (ACP) as compared to 16:0-ACP, and the fatty acid moieties in the soybean oil from said soybean seed comprise greater than about 20% stearate and less than about 53% stearate. 2. The soybean seed of claim 1, wherein said DNA sequence encoding a plant FatA thioesterase is SEQ ID NO:1. 3. The soybean seed of claim 1, wherein said introduced nucleic acid sequence has a transcription termination sequence in the 3' direction of said DNA sequence encoding a plant FatA thioesterase. 4. The soybean seed of claim 1, wherein said soybean oil contains about 50 weight percent or greater stearate expressed as a component of total fatty acids contained in the soybean seed oil. 5. The soybean seed of claim 1, wherein said oil comprises 25 percent or greater of said total fatty acids in a saturated-unsaturated-saturated form. 6. The soybean seed of claim 1, wherein said plant FatA thioesterase 16:0 activity represents only approximately 3% of said plant FatA thioesterase 18:1 activity. 7. The soybean seed of claim 1, wherein said fatty acid moieties including 16:0, 18:0, 20:0, and 22:0 comprise at least 30 percent of the total fatty acids as a percentage of weight. 8. The soybean seed of claim 1, wherein said fatty acid moieties including 16:0, 18:0, 20:0, and 22:0 comprise at least 50 percent of the total fatty acids as a percentage of weight. 9. The soybean seed of claim 1, wherein the percentage of saturated fatty acid moieties greater than 18:0 are increased as compared to null progeny which do not contain the Garm FatA1 transgene. 10. The soybean seed of claim 1, wherein stearate levels increase 4 fold to approximately 7 fold as compared to seeds from nontransformed control plants. 11. The soybean seed of claim 1, wherein said promoter functional in a soybean plant seed cell is selected from the group consisting of an ACP transcriptional initiation control region, a napin transcriptional initiation control region, a β-conglycinin 7S transcriptional initiation control region, Lesquerella hydroxylase promoter, and a stearoyl-ACP desaturase promoter. 12. The soybean seed of claim 11, wherein said promoter that is functional in a soybean seed cell is said β-conglycinin 7S transcriptional initiation control region. 13. The soybean seed of claim 11, wherein said promoter that is functional in a soybean seed cell is said napin transcriptional initiation control region. 14. The soybean seed of claim 1, wherein said promoter that is functional in a soybean seed cell is preferentially expressed in said soybean cell. 15. The soybean seed of claim 1, wherein said encoding sequence has no activity on 16:0 substrates. 16. The soybean seed of claim 1, wherein said promoter is from a gene preferentially expressed in plant seed tissue. 17. The soybean seed of claim 1, wherein said DNA sequence encoding a plant FatA thioesterase is from the genus Garcinia. 18. The soybean seed of claim 1, wherein said fatty acid moieties in the soybean oil from said soybean seed comprise about 40% by weight stearate. 19. The soybean seed according to claim 1 comprising about 50 percent or greater stearate as the fatty acid found at the sn-1 and sn-3 positions of seed triglycerides. 20. The soybean seed according to claim 18 comprising about 60 percent or greater stearate as the fatty acid found in said sn-1 and sn-3 positions of seed triglycerides. 21. The soybean seed according to claim 4 comprising about 75 percent or greater stearate as the fatty acid found in said sn-1 and sn-3 positions of seed triglycerides. 22. The soybean seed according to claim 19, and comprising oleic acid as the predominant fatty acid at the sn-2 position of seed triglycerides.
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이 특허에 인용된 특허 (19)
McCabe Dennis E. (Middleton WI) Martinell Brian J. (Madison WI), Apparatus for genetic transformation.
Christou Paul (Madison WI) McCabe Dennis (Middleton WI) Swain William F. (Madison WI) Barton Kenneth A. (Middleton WI), Particle-mediated transformation of soybean plants and lines.
Hitz William Dean ; Yadav Narendra S., Specific for palmitoyl, stearoyl and oleoyl-alp thioesters nucleic acid fragments encoding acyl-acp thiosesterase enzyme.
Nikolau, Basil J.; Yandeau-Nelson, Marna; Jing, Fuyuan, Materials and methods for using an acyl-acyl carrier protein thioesterase and mutants and chimeras thereof in fatty acid synthesis.
Nikolau, Basil J.; Yandeau-Nelson, Marna; Jing, Fuyuan, Materials and methods for using an acyl-acyl carrier protein thioesterase and mutants and chimeras thereof in fatty acid synthesis.
Nikolau, Basil J.; Yandeau-Nelson, Marna; Jing, Fuyuan, Materials and methods for using an acyl—acyl carrier protein thioesterase and mutants and chimeras thereof in fatty acid synthesis.
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