IPC분류정보
국가/구분 |
United States(US) Patent
등록
|
국제특허분류(IPC7판) |
|
출원번호 |
UP-0529197
(2003-09-26)
|
등록번호 |
US-7521261
(2009-07-01)
|
국제출원번호 |
PCT/US03/030623
(2003-09-26)
|
§371/§102 date |
20060130
(20060130)
|
국제공개번호 |
WO04/029217
(2004-04-08)
|
발명자
/ 주소 |
- Haselton, Rick
- McQuain, Mark
|
출원인 / 주소 |
|
대리인 / 주소 |
Fulbright & Jaworski, LLP
|
인용정보 |
피인용 횟수 :
2 인용 특허 :
9 |
초록
Systems and methods are described for screening molecular interactions using a filamentous based platform for molecule presentation. Method includes screening for protein-protein, DNA-DNA and other chemical interactions.
대표청구항
▼
The invention claimed is: 1. A method of detecting target-probe interactions comprising: (a) providing a filament with a plurality of a first probe disposed in an annular fashion thereon; (b) traversing the filament through a first chamber, wherein the first chamber contains the target in solution;
The invention claimed is: 1. A method of detecting target-probe interactions comprising: (a) providing a filament with a plurality of a first probe disposed in an annular fashion thereon; (b) traversing the filament through a first chamber, wherein the first chamber contains the target in solution; and (c) assessing binding of the target to a member of the plurality of the first probe. 2. The method of claim 1, wherein the plurality of the first probe is associated with a probe identifier. 3. The method of claim 2, wherein the probe identifier is a bar code. 4. The method of claim 3, wherein the bar code is disposed in an annular fashion. 5. The method of claim 3, wherein the bar code is disposed in a linear fashion. 6. The method of claim 1, wherein the filament has a plurality of different probes disposed thereon. 7. The method of claim 6, wherein the plurality of different probes are disposed in a single ring around the filament. 8. The method of claim 7, wherein each of the plurality of different probes is associated with a distinct probe identifier. 9. The method of claim 1, further comprising traversing the filament through a second chamber, wherein the second chamber contains a solution that lacks the target. 10. The method of claim 9, wherein the second chamber comprises a solution for pre-processing or post-processing of the filament. 11. The method of claim 10, wherein the preprocessing comprises making an array, chemical blocking of a reactive group on the target, ionic blocking of a target, or denaturing of a target. 12. The method of claim 10, wherein the post-processing comprises deblocking of a reactive group on the target, removal of an ionic blocker, or renaturing of a target molecule. 13. The method of claim 1, wherein the target is labeled with a fluorescent label, a chemilluminescent label, a radioactive label, a magnetic label, or a spin resonance label. 14. The method of claim 1, further comprising convective transport of the target solution by means of filament movement through the first chamber. 15. The method of claim 14, further comprising re-circulating target solution from the first chamber. 16. The method of claim 1, wherein the filament comprises surface features to enhance mixing of the target solution. 17. The method of claim 1, wherein the first chamber comprises surface features to enhance mixing of the target solution. 18. The method of claim 1, wherein the filament is transparent. 19. The method of claim 1, wherein the filament is adapted to incorporate an electrical charge. 20. The method of claim 19, further comprising subjecting the target to electrophoretic movement. 21. The method of claim 20, wherein the electrophoretic movement promotes target-probe interaction. 22. The method of claim 20, wherein the electrophoretic movement inhibits target-probe interaction. 23. The method of claim 1, further comprising a second traversing of the filament through a chamber comprising the target. 24. The method of claim 23, wherein the chamber used for the second traversing is the same chamber in step (b). 25. The method of claim 23, wherein the chamber used for the second traversing is a different chamber than in step (b). 26. The method of claim 23, wherein a temperature in the chamber used for the second traversing is altered from that used in step (b). 27. The method of claim 23, wherein a charge in the chamber used for the second traversing is altered from that used in step (b). 28. The method of claim 23, wherein a current, amperage, voltage or polarity in the chamber used for the second traversing is altered from that used in step (b). 29. The method of claim 1, further comprising enhancing detection of binding of the target to the first probe. 30. The method of claim 29, wherein enhancing comprises traversing the filament through a second processing chamber that contains (i) a second liquid phase probe that binds to the target at a location distinct from the first probe, and wherein the second liquid phase probe contains a binding site for a third liquid phase probe; and (ii) a third liquid phase probe that is detectable. 31. The method of claim 30, wherein the third liquid phase probe is provided in an inactive state and then activated to facilitate amplification. 32. The method of claim 31, wherein the third liquid phase probe is labeled with a fluorescent, a chemilluminescent or a radioactive molecule. 33. The method of claim 31, wherein the third liquid phase probe is a linear molecule with a binding site for itself. 34. The method of claim 31, wherein the third liquid phase probe is a branched molecule with multiple binding sites for itself. 35. The method of claim 1, wherein the filament is 1 μm to about 0.5 cm in diameter. 36. The method of claim 1, wherein the processing chamber is greater than 1 μm in diameter and less than 2.0 cm. 37. The method of claim 1, wherein the target solution in the processing chamber is present in a volume of less than 100 μl. 38. The method of claim 1, wherein the filament comprises an axial or radial probe density of greater than 1 probe region per cm. 39. A method of detecting target-probe interactions comprising: (a) providing a filament with a plurality of different probes disposed thereon; (b) traversing the filament through a first chamber, wherein the first chamber contains the target in solution; and (c) assessing binding of the target to more than one of the probes. 40. The method of claim 39, wherein the plurality of probes are disposed on said filament in annular fashion. 41. The method of claim 39, wherein the plurality of probes is associated with a probe identifier. 42. The method of claim 41, wherein the probe identifier is a bar code. 43. The method of claim 42, wherein the bar code is disposed in a linear fashion. 44. The method of claim 39, wherein each of the plurality of different probes is associated with a distinct probe identifier. 45. The method of claim 39, further comprising traversing the filament through a second chamber, wherein the second chamber contains a solution that lacks the target. 46. The method of claim 45, wherein the second chamber comprises a solution for pre-processing or post-processing of the filament. 47. The method of claim 46, wherein the preprocessing comprises making an array, chemical blocking of a reactive group on the target, ionic blocking of a target, or denaturing of a target. 48. The method of claim 46, wherein the post-processing comprises deblocking of a reactive group on the target, removal of an ionic blocker, or renaturing of a target molecule. 49. The method of claim 39, wherein the target is labeled with a fluorescent label, a chemilluminescent label, a radioactive label, a magnetic label, or a spin resonance label. 50. The method of claim 39, wherein the bar code is disposed in an annular fashion. 51. The method of claim 39, further comprising convective transport of the target solution by means of filament movement through the first chamber. 52. The method of claim 51, further comprising re-circulating target solution from the first chamber. 53. The method of claim 39, wherein the filament comprises surface features to enhance mixing of the target solution. 54. The method of claim 39, wherein the first chamber comprises surface features to enhance mixing of the target solution. 55. The method of claim 39, wherein the filament is transparent. 56. The method of claim 39, wherein the filament is adapted to incorporate an electrical charge. 57. The method of claim 39, further comprising subjecting the target to electrophoretic movement. 58. The method of claim 57, wherein the electrophoretic movement promotes target-probe interaction. 59. The method of claim 57, wherein the electrophoretic movement inhibits target-probe interaction. 60. The method of claim 39, further comprising a second traversing of the filament through a chamber comprising the target. 61. The method of claim 60, wherein the chamber used for the second traversing is the same chamber in step (b). 62. The method of claim 60, wherein the chamber used for the second traversing is a different chamber than in step (b). 63. The method of claim 60, wherein a temperature in the chamber used for the second traversing is altered from that used in step (b). 64. The method of claim 60, wherein a charge in the chamber used for the second traversing is altered from that used in step (b). 65. The method of claim 60, wherein a current, amperage, voltage or polarity in the chamber used for the second traversing is altered from that used in step (b). 66. The method of claim 39, further comprising enhancing detection of binding of the target to one of the probes. 67. The method of claim 66, wherein enhancing comprises traversing the filament through a second processing chamber that contains (i) a second liquid phase probe that binds to the target at a location distinct from the first probe, and wherein the second liquid phase probe contains a binding site for a third liquid phase probe; and (ii) a third liquid phase probe that is detectable. 68. The method of claim 67, wherein the third liquid phase probe is provided in an inactive state and then activated to facilitate amplification. 69. The method of claim 68, wherein the third liquid phase probe is labeled with a fluorescent, a chemilluminescent or a radioactive molecule. 70. The method of claim 68, wherein the third liquid phase probe is a linear molecule with a binding site for itself. 71. The method of claim 68, wherein the third liquid phase probe is a branched molecule with multiple binding sites for itself. 72. The method of claim 39, wherein the filament is 1 μm to about 0.5 cm in diameter. 73. The method of claim 39, wherein the processing chamber is greater than 1 μm in diameter and less than 2.0 cm. 74. The method of claim 39, wherein the target solution in the processing chamber is present in a volume of less than 100 μl. 75. The method of claim 39, wherein the filament comprises an axial or radial probe density of greater than 1 probe region per cm.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.