IPC분류정보
국가/구분 |
United States(US) Patent
등록
|
국제특허분류(IPC7판) |
|
출원번호 |
UP-0513501
(2006-08-31)
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등록번호 |
US-7531319
(2009-07-01)
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발명자
/ 주소 |
- Martin, Stephanie M.
- MacDonald, J. Gavin
- Lye, Jason
- Sayre, Curtis
- Thompson, Kimberlee
|
출원인 / 주소 |
- Kimberly Clark Worldwide, Inc.
|
대리인 / 주소 |
|
인용정보 |
피인용 횟수 :
6 인용 특허 :
46 |
초록
▼
A method for detecting a microorganism or class of microorganisms is provided. More specifically, the method employs an array that contains a plurality of discrete regions (referred to as "addresses") spaced apart on a solid support in a predetermined pattern. The addresses are selected so that the
A method for detecting a microorganism or class of microorganisms is provided. More specifically, the method employs an array that contains a plurality of discrete regions (referred to as "addresses") spaced apart on a solid support in a predetermined pattern. The addresses are selected so that the array provides a distinct spectral response (e.g., pattern of colors) or "fingerprint" for a particular microorganism or class of microorganisms. For example, the array may provide a certain spectral response in the presence of one microorganism or class of microorganisms (e.g., gram-negative bacteria), but provide a completely different spectral response in the presence of another microorganism or class of microorganisms (e.g., gram-positive bacteria). Detection of the spectral response provided by the array may thus allow for differentiation between microorganisms.
대표청구항
▼
What is claimed is: 1. A method for detecting a microorganism in a sample, the method comprising: contacting the sample with an array, the array comprising a plurality of individual array addresses spaced apart in a predetermined pattern on a solid support, wherein the addresses each contain a colo
What is claimed is: 1. A method for detecting a microorganism in a sample, the method comprising: contacting the sample with an array, the array comprising a plurality of individual array addresses spaced apart in a predetermined pattern on a solid support, wherein the addresses each contain a colorant so that the array produces a visually observable spectral response; detecting the spectral response; and correlating the detected spectral response to the presence of one or more microorganisms. 2. The method of claim 1, wherein one or more of the addresses contain a pH-sensitive colorant. 3. The method of claim 2, wherein the pH sensitive colorant is a phthalein, hydroxyanthraquinone, arylmethane, aromatic azo, or a derivative thereof. 4. The method of claim 1, wherein one or more of the addresses contain a metal complexing colorant. 5. The method of claim 1, wherein one or more of the addresses contain a solvatochromoatic colorant. 6. The method of claim 1, wherein the array contains from 2 to 50 individual array addresses. 7. The method of claim 1, wherein the array contains from 3 to 40 individual array addresses. 8. The method of claim 1, wherein at least two of the addresses are spaced apart a distance of from about 0.01 to about 100 millimeters. 9. The method of claim 1, wherein at least two of the addresses are spaced apart a distance of from about 0.1 to about 50 millimeters. 10. The method of claim 1, wherein the spectral response is correlated to the presence of one or more microorganisms at a concentration of about 1×103 or more colony forming units per milliliter of the sample. 11. The method of claim 1, wherein the spectral response is correlated to the presence of one or more microorganisms at a concentration of about 1×106 or more colony forming units per milliliter of the sample. 12. The method of claim 1, wherein the colorant is applied to the solid support in the form of a composition that comprises a carrier. 13. The method of claim 12, wherein the colorant constitutes from about 0.1 to about 100 milligrams per milliliter of the carrier. 14. The method of claim 12, wherein the colorant constitutes from about 0.5 to about 60 milligrams per milliliter of the carrier. 15. The method of claim 1, wherein the array is printed onto the support. 16. The method of claim 1, wherein the spectral response is detected and compared to a control array. 17. The method of claim 1, wherein the spectral response is visually detected. 18. The method of claim 1, wherein the spectral response is produced in about 30 minutes or less after the array is contacted with the sample. 19. The method of claim 1, wherein the spectral response is produced in about 5 minutes or less after the array is contacted with the sample. 20. The method of claim 1, wherein the microorganism is a fungus. 21. The method of claim 20, wherein the fungus is C. albicans, A. pullulans, P. janthinellum, or a mixture thereof. 22. The method of claim 1, wherein the microorganism is a bacteria. 23. The method of claim 22, wherein the bacteria is a gram-positive bacteria. 24. The method of claim 23, wherein the gram-positive bacteria is S. aureus, L. acidophilus, S. epidermidis, B. subtilis, E. faecalis, S. pyogenes, S. pneumoniae, or a mixture thereof. 25. The method of claim 22, wherein the bacteria is a gram-negative bacteria. 26. The method of claim 25, wherein the gram-negative bacteria is E. coli, P. aeruginosa, K. pneumoniae, P. mirabilis, M. lacunata, H. influenzae, or a mixture thereof.
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