초록 ▼

The vascular endothelial growth factor (VEGF) activity in a patient's bloodstream or other biological sample can serve as a diagnostic and prognostic index for cancer, diabetes, heart conditions, and other pathologies. Antibody-sandwich ELISA method and kits for VEGF as an antigen were developed to

The vascular endothelial growth factor (VEGF) activity in a patient's bloodstream or other biological sample can serve as a diagnostic and prognostic index for cancer, diabetes, heart conditions, and other pathologies. Antibody-sandwich ELISA method and kits for VEGF as an antigen were developed to detect VEGF levels in biological samples from animal models and human patients and are used as a diagnostic/prognostic index.

대표청구항 ▼

What is claimed is: 1. A method for detecting multiple isoforms of vascular endothelial growth factor (VEGF) in a biological sample, comprising: (a) incubating a biological sample with a capture reagent immobilized on a solid support to bind multiple isoforms of VEGF to the capture reagent at a pH

What is claimed is: 1. A method for detecting multiple isoforms of vascular endothelial growth factor (VEGF) in a biological sample, comprising: (a) incubating a biological sample with a capture reagent immobilized on a solid support to bind multiple isoforms of VEGF to the capture reagent at a pH of about 6.0 to about 9.5, wherein the capture reagent comprises a mixture comprising a polyclonal antibody that binds VEGF and a monoclonal antibody that specifically binds to amino acid residues 111-165 of human VEGF; and (b) detecting VEGF bound to the immobilized capture reagent by contacting the bound VEGF with a detectable antibody that binds to N-terminal amino acid residues 1-110 of VEGF. 2. The method of claim 1, wherein the pH is about 6.0 to about 7.0. 3. The method of claim 2, wherein the pH is about 6.0 to about 6.5. 4. The method of claim 3, wherein the pH is about 6.35. 5. The method of claim 1, wherein the incubation of step (a) is at a temperature of about 0° C. to about 40° C. 6. The method of claim 5, wherein the incubation is at a temperature of about 36° C. to about 38° C. 7. The method of claim 1, wherein the incubation of step (a) is about 0.5 to about 3.0 hours. 8. The method of claim 7, wherein the incubation is about 1.5 to about 3.0 hours. 9. The method of claim 1, wherein the incubation of step (a) is not greater than about 10 hours.