Genes encoding novel proteins with pesticidal activity against coleopterans
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
A01H-005/00
A01H-005/10
C12N-015/82
C12N-015/32
출원번호
UP-0032717
(2001-10-23)
등록번호
US-7605304
(2009-11-10)
발명자
/ 주소
Abad, Andre R.
Duck, Nicholas B.
Feng, Xiang
Flannagan, Ronald D.
Kahn, Theodore W.
Sims, Lynne E.
출원인 / 주소
E.I. du Pont de Nemours and Company
대리인 / 주소
Alston & Bird LLP
인용정보
피인용 횟수 :
3인용 특허 :
4
초록▼
The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding δ-endotoxins having pesticidal activity against pests of the order Coleoptera. The invention further provides mutagenized nucleic acids that have been modified to e
The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding δ-endotoxins having pesticidal activity against pests of the order Coleoptera. The invention further provides mutagenized nucleic acids that have been modified to encode endotoxins having improved pesticidal activity and/or altered pest specificity. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, expression cassettes comprising such nucleic acids, and transformed plants and seeds comprising a nucleic acid of the invention. These compositions find use in methods for controlling pests, especially plant pests.
대표청구항▼
That which is claimed: 1. An isolated nucleic acid comprising a nucleotide sequence that encodes an amino acid sequence having at least 90% sequence identity to the amino acid sequence set forth in SEQ ID NO:2, wherein said nucleotide sequence encodes a polypeptide which is pesticidal for at least
That which is claimed: 1. An isolated nucleic acid comprising a nucleotide sequence that encodes an amino acid sequence having at least 90% sequence identity to the amino acid sequence set forth in SEQ ID NO:2, wherein said nucleotide sequence encodes a polypeptide which is pesticidal for at least one pest selected from the group consisting of western corn rootworm, southern corn rootworm, Colorado potato beetle, and boll weevil. 2. The nucleic acid according to claim 1, wherein the nucleotide sequence is optimized for expression in a plant. 3. An expression cassette comprising a nucleic acid according to claim 1, wherein said nucleotide sequence is operably linked to a promoter that drives expression in a microorganism or in a plant cell. 4. The nucleic acid of claim 1, wherein said nucleotide sequence encodes an amino acid sequence having at least 95% sequence identity to the amino acid sequence set forth in SEQ ID NO:2. 5. The nucleic acid of claim 1, wherein said nucleotide sequence encodes the amino acid sequence set forth in SEQ ID NO:2. 6. The nucleic acid of claim 1, wherein said nucleotide sequence is set forth in SEQ ID NO:1. 7. The nucleic acid of claim 1, wherein said nucleotide sequence encodes an amino acid sequence having at least 93% sequence identity to the amino acid sequence set forth in SEQ ID NO:2. 8. The nucleic acid of claim 1, wherein said nucleotide sequence encodes an amino acid sequence having at least 94% sequence identity to the amino acid sequence set forth in SEQ ID NO:2. 9. The nucleic acid of claim 1, wherein said nucleotide sequence is optimized for expression in a plant. 10. A transformed plant comprising in its genome at least one stably incorporated nucleotide construct comprising a nucleotide sequence encoding a polypeptide operably linked to a promoter that drives expression of said polypeptide, wherein said polypeptide is pesticidal for at least one pest selected from the group consisting of western corn rootworm, southern corn rootworm, Colorado potato beetle, and boll weevil and wherein said nucleotide sequence encodes an amino acid sequence having at least 90% sequence identity to the amino acid sequence set forth in SEQ ID NO:2. 11. The plant according to claim 10, wherein the plant is a monocot. 12. The plant according to claim 10, wherein said plant is a dicot. 13. Transformed seed of the plant according to claim 10. 14. The transformed plant of claim 10, wherein said nucleotide sequence encodes an amino acid sequence having at least 95% sequence identity to the amino acid sequence set forth in SEQ ID NO:2. 15. The transformed plant of claim 10, wherein said nucleotide sequence encodes the amino acid sequence set forth in SEQ ID NO:2. 16. The transformed plant of claim 10, wherein said nucleotide sequence is set forth in SEQ ID NO:1. 17. The transformed plant of claim 10, wherein said nucleotide sequence is optimized for expression in a plant. 18. The transformed plant of claim 11, wherein said nucleotide sequence encodes an amino acid sequence having at least 93% sequence identity to the amino acid set forth in SEQ ID NO:2. 19. The plant of claim 18, wherein said plant is a dicot. 20. The plant of claim 18, wherein said plant is a monocot. 21. The plant of claim 18, wherein said monocot is maize. 22. The transformed plant of claim 11, wherein said nucleotide sequence encodes an amino acid sequence having at least 94% sequence identity to the amino acid set forth in SE ID NO:2. 23. A method for impacting an insect pest comprising introducing into a plant or cell thereof at least one nucleotide construct comprising a nucleotide sequence encoding a polypeptide operably linked to a promoter that drives expression of said polypeptide in plant cells, wherein said polypeptide is pesticidal for at least one pest selected from the group consisting of western corn rootworm, southern corn rootworm, Colorado potato beetle, and boll weevil and wherein said nucleotide sequence encodes an amino acid sequence having at least 90% sequence identity to the amino acid sequence set forth in SEQ ID NO:2, whereby an insect pest feeding on said plant or cell thereof is impacted. 24. The method according to claim 23, wherein the plant produces a polypeptide characterized by pesticidal activity against at least one pest selected from the group consisting of western corn rootworm, southern corn rootworm, Colorado potato beetle, and boll weevil. 25. The method of claim 23, wherein said nucleotide sequence encodes an amino acid sequence having at least 95% sequence identity to the amino acid sequence set forth in SEQ ID NO:2. 26. The method of claim 23, wherein said nucleotide sequence encodes the amino acid sequence set forth in SEQ ID NO:2. 27. The method of claim 23, wherein said nucleotide sequence is set forth in SEQ ID NO:1. 28. The method of claim 23, wherein said nucleotide sequence is optimized for expression in a plant. 29. The method of claim 23, wherein said nucleotide sequence encodes an amino acid sequence having at least 93% sequence identity to the amino acid sequence set forth in SEQ ID NO:2. 30. The method of claim 23, wherein said nucleotide sequence encodes an amino acid sequence having at least 94% sequence identity to the amino acid sequence set forth in SEQ ID NO:2.
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이 특허에 인용된 특허 (4)
Michaels Tracy E. (Ames IA) Narva Kenneth E. (San Diego CA) Foncerrada Luis (Vista CA), Bacillus thuringiensis toxins active against scarab pests.
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