초록 ▼

It is an object of the present invention to provide magnetic substance-encapsulated particles which have uniform magnetism, high dispersion stability and a narrow particle size distribution, a method of producing the same, particles for immunoassay formed by using the magnetic substance-encapsulated

It is an object of the present invention to provide magnetic substance-encapsulated particles which have uniform magnetism, high dispersion stability and a narrow particle size distribution, a method of producing the same, particles for immunoassay formed by using the magnetic substance-encapsulated particles and a method of immunoassay in which the magnetic substance-encapsulated particles or the particles for immunoassay are used. The present invention relates to a magnetic substance-encapsulated particle, which comprises an organic polymer material and a magnetic substance having an average particle size of 1 to 30 nm, the magnetic substance being contained within a particle in a state of being dispersed.

대표청구항 ▼

The invention claimed is: 1. A magnetic substance-encapsulated particle, which comprises an organic polymer material and a magnetic substance, wherein the magnetic substance has an average particle size of 1 to 30 nm, the magnetic substance being dispersed and encapsulated within the organic polyme

The invention claimed is: 1. A magnetic substance-encapsulated particle, which comprises an organic polymer material and a magnetic substance, wherein the magnetic substance has an average particle size of 1 to 30 nm, the magnetic substance being dispersed and encapsulated within the organic polymer material of said particle; wherein said organic polymer material comprises a monomer not having a hydrophilic group for forming a core of said particle and a monomer having a hydrophilic group for forming a shell of said particle and forming a particle having dispersion stability in water; and wherein an absolute deviation of a component ratio between a carbon element comprising the organic polymer material and a metal element comprising the magnetic substance is 0.27 or less, wherein the monomer not having a hydrophilic group comprises a monomer having a glycidyl group and a styrenic monomer and wherein the monomer having a hydrophilic group is polyethylene glycol (meth)acrylate represented by the following general formula (1) or a compound represented by the following general formula (2): CH2═CR--COO--(CH2--CH2--O) n--H (1) in the formula, R represents H or CH3, and n represents an integer of 1 to 20, in the formula, X represents H or SO3-NH4+, and n represents an integer of 3 to 30. 2. The magnetic substance-encapsulated particle according to claim 1, wherein the magnetic substance is formed by oxidization of a metal ion within a particle in a polymerization process of forming the magnetic substance-encapsulated particle. 3. The magnetic substance-encapsulated particle according to claim 2, wherein the metal ion is an iron ion. 4. The magnetic substance-encapsulated particle according to claim 1, wherein the proportion of a monomer unit derived from the styrenic monomer in the organic polymer material is 5 to 90% by weight. 5. The magnetic substance-encapsulated particle according to claim 1, wherein the organic polymer material is crosslinked. 6. The magnetic substance-encapsulated particle according to claim 1, which has at least a functional group selected from the group consisting of a carboxyl group, a hydroxyl group, an epoxy group, an amino group, a triethylammonium group, a dimethylamino group and a sulfonic acid group at the surface of the particle. 7. The magnetic substance-encapsulated particle according to claim 1, wherein an average particle size is 0.05 to 1 μm. 8. The magnetic substance-encapsulated particle according to claim 1, wherein a content of the magnetic substance is 0.1 to 50% by weight. 9. The magnetic substance-encapsulated particle according to claim 1, wherein an average particle size of the magnetic substance is 2 to 10 nm. 10. The magnetic substance-encapsulated particle according to claim 1, wherein a linker having a functional group capable of forming a covalent bond with an antigen or an antibody binds to a particle surface. 11. The magnetic substance-encapsulated particle according to claim 10, wherein the functional group capable of forming a covalent bond with an antigen or an antibody is an epoxy group. 12. The magnetic substance-encapsulated particle according to claim 10, wherein the linker is polyethylene glycol diglycidyl ether.