IPC분류정보
국가/구분 |
United States(US) Patent
등록
|
국제특허분류(IPC7판) |
|
출원번호 |
UP-0576406
(2005-09-30)
|
등록번호 |
US-7634917
(2010-01-08)
|
우선권정보 |
DE-10 2004 047 965(2004-10-01) |
국제출원번호 |
PCT/EP05/054947
(2005-09-30)
|
§371/§102 date |
20070413
(20070413)
|
국제공개번호 |
WO06/037760
(2006-04-13)
|
발명자
/ 주소 |
- Fuhr, Günter
- Zimmermann, Heiko
- Oh, Young Joo
- Schön, Uwe
|
출원인 / 주소 |
- Fraunhofer Gesellschaft zur Forderung der Angewandten Forschung E.V.
|
대리인 / 주소 |
Caesar, Rivise, Bernstein, Cohen & Pokotilow, Ltd.
|
인용정보 |
피인용 횟수 :
0 인용 특허 :
10 |
초록
▼
The invention relates to a cryoapparatus for freezing and/or thawing a sample (1), especially in the cryopreservation of a biological sample (1), with a coolable cooling space (3) and with a sample container (10) arranged in the cooling space (3) for temporarily receiving the sample (1) when freezin
The invention relates to a cryoapparatus for freezing and/or thawing a sample (1), especially in the cryopreservation of a biological sample (1), with a coolable cooling space (3) and with a sample container (10) arranged in the cooling space (3) for temporarily receiving the sample (1) when freezing or thawing the sample (1). It is suggested that the sample container (10) can be tempered separately from the cooling space. Furthermore, the invention includes an associated operating method.
대표청구항
▼
The invention claimed is: 1. A cryoapparatus for freezing and thawing a sample, comprising: (a) a coolable cooling space, (b) a sample container arranged in the cooling space for temporarily receiving the sample when freezing or thawing the sample, (c) a thermally insulated, extractable transport c
The invention claimed is: 1. A cryoapparatus for freezing and thawing a sample, comprising: (a) a coolable cooling space, (b) a sample container arranged in the cooling space for temporarily receiving the sample when freezing or thawing the sample, (c) a thermally insulated, extractable transport container that is arranged in the cooling space for extracting the sample from the cooling space after the freezing and for inserting the sample into the cooling space for thawing, (d) sample transferring means for transferring within the cooling space the sample out of the sample container and into the transport container and out of the transport container and into the sample container, (e) temperature adjusting means for adjusting a temperature of the sample container separately from the cooling space between the insertion of the sample and the extraction of the sample, and (f) cooling means for cooling a lower area of the cooling space to a given target temperature of a freezing process so as to cool the sample container from a given starting temperature of the freezing process to the target temperature of the freezing process. 2. The cryoapparatus according to claim 1, further comprising a lifting apparatus with which the sample container can be lowered and raised in the cooling space in a controlled or regulated manner. 3. The cryoapparatus according to claim 1, wherein the sample container has a temperature sensor that measures a temperature in the sample container. 4. The cryoapparatus according to claim 3, wherein several temperature sensors are attached to the sample container at different vertical distances in order to determine a local vertical temperature gradient in the cooling space. 5. The cryoapparatus according to claim 1, wherein the sample container is stationarily arranged on a bottom of the cooling space. 6. The cryoapparatus according to claim 1, further comprising a heating apparatus for heating the cooling space. 7. The cryoapparatus according to claim 6, wherein the heating apparatus heats only an upper partial area of the cooling space. 8. The cryoapparatus according to claim 1, further comprising a vertical temperature stratification in the cooling space with a lower cold layer and an upper warm layer. 9. The cryoapparatus according to claim 8, wherein the warm layer has a temperature that corresponds substantially to a given starting temperature of a freezing process whereas the cold layer has a temperature that corresponds substantially to a given target temperature of the freezing process. 10. The cryoapparatus according to claim 1, wherein the cooling space is cooled by a first cooling apparatus and the sample container is cooled by a second cooling apparatus. 11. The cryoapparatus according to claim 10, further comprising a control apparatus, wherein the control apparatus is connected on an output side to the first cooling apparatus and to the second cooling apparatus and adjusts a cooling capacity of the cryoapparatus, whereas the control apparatus is connected on an input side to a temperature sensor of the sample container. 12. The cryoapparatus according to claim 1, wherein the sample container is thermally insulated and has a cover that can be opened in order to extract the sample and in order to insert the sample and can be closed in order to thermally insulate the sample container during cooling. 13. The cryoapparatus according to claim 1, wherein the sample container is not thermally insulated against the cooling space. 14. The cryoapparatus according to claim 1, wherein a substantially vertically running shaft with a wall of a heat-conducting material is arranged in the cooling space, wherein the sample container can move vertically in the shaft. 15. The cryoapparatus according to claim 1, wherein the transport container is arranged on a bottom of the cooling space. 16. The cryoapparatus according to claim 1, wherein the cooling space is vat-shaped and is covered on top by a removable protective hood. 17. The cryoapparatus according to claim 16, wherein the protective hood is at least partially transparent. 18. The cryoapparatus according to claim 1, wherein several storage surfaces are arranged in the cooling space at different heights that correspond to different temperatures. 19. An operating method for a cryoapparatus, comprising the following steps: (a) insertion of the sample into a sample container arranged in a coolable cooling space, (b) adjusting a temperature of the sample present in the sample container, (c) extraction of the sample from the sample container, wherein the sample container is temperature adjusted separately from the cooling space between the insertion of the sample and the extraction of the sample, and in order to freeze the sample: a lower area of the cooling space is cooled down to a given target temperature of a freezing process, the sample container is cooled down from a given starting temperature of the freezing process to the target temperature of the freezing process, the sample is transferred, after attaining the target temperature, into a thermally insulated transport container located in the cooling space and the transport container containing the sample is extracted from the cooling space, and in order to thaw the sample: the lower area of the cooling space is cooled down to a given target temperature of a freezing process, a thermally insulated transport container containing the sample is inserted into the cooling space, the sample is transferred out of the transport container into the sample container, the sample container is heated from a given starting temperature of a thawing process to a given target temperature of the thawing process, and the sample is extracted from the sample container after the target temperature of the thawing process has been attained. 20. The operating method according to claim 19, wherein the sample container is raised and lowered in the cooling space. 21. The operating method according to claim 19, wherein a temperature of the sample container is adjusted by raising or lowering the sample container in the cooling space in a controlled or regulated manner. 22. The operating method according to claim 19, wherein the sample container is actively cooled by a cooling apparatus. 23. The operating method according to claim 19, wherein a vertical temperature stratification with an upper warm layer and a lower cold layer is generated in the cooling space. 24. The operating method according to claim 23, wherein the warm layer is brought to a temperature that corresponds substantially to a given starting temperature of a freezing process, whereas the cold layer is brought to a temperature that corresponds substantially to a given target temperature of the freezing process. 25. The operating method according to claim 19, wherein the cooling space is actively heated. 26. The operating method according to claim 25, wherein the cooling space is actively heated only in an upper partial area. 27. The cryoapparatus according to claim 8, wherein the warm layer has a temperature that corresponds substantially to a given target temperature of a thawing process whereas the cold layer has a temperature that corresponds substantially to a given starting temperature of the thawing process. 28. The operating method according to claim 23, wherein the warm layer is brought to a temperature that corresponds substantially to a given target temperature of a thawing process, whereas the cold layer is brought to a temperature that corresponds substantially to a given starting temperature of the thawing process.
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