Semen extender composition and methods for manufacturing and using
원문보기
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
A01N-001/02
A61B-017/43
출원번호
UP-0044796
(2002-01-11)
등록번호
US-7674576
(2010-04-21)
발명자
/ 주소
Loskutoff, Naida M.
Rohr, Jennifer
Lomneth, Richard B.
Wood, David G.
Crichton, Elizabeth
출원인 / 주소
ABS Corporation
대리인 / 주소
Merchant & Gould, P.A.
인용정보
피인용 횟수 :
1인용 특허 :
16
초록▼
A semen extender composition is provided. The semen extender composition includes a sperm cell protecting amount of phospholipid, an effective amount of surfactant to reduce ice crystal formation during freezing of the composition; a carbohydrate, and a biological buffer to provide a semen extender
A semen extender composition is provided. The semen extender composition includes a sperm cell protecting amount of phospholipid, an effective amount of surfactant to reduce ice crystal formation during freezing of the composition; a carbohydrate, and a biological buffer to provide a semen extender use solution having a pH of between about 6.9 and about 7.5, and wherein the use solution exhibits an osmolality of about 250 mOsM to about 350 mOsM. Methods for manufacturing and using a semen extender composition are provided.
대표청구항▼
We claim: 1. An aqueous ready to use semen extender composition comprising: (a) about 0.1 wt. % to about 6 wt. % phospholipid obtained from a non-animal source comprising lecithin; (b) about 0.0001 wt. % to about 1 wt. % of anionic surfactant to reduce ice crystal formation during freezing of the c
We claim: 1. An aqueous ready to use semen extender composition comprising: (a) about 0.1 wt. % to about 6 wt. % phospholipid obtained from a non-animal source comprising lecithin; (b) about 0.0001 wt. % to about 1 wt. % of anionic surfactant to reduce ice crystal formation during freezing of the composition, wherein the anionic surfactant comprises a sulfate; (c) about 0.5 wt. % to about 3 wt. % carbohydrate; (d) about 3 wt. % to about 14 wt. % freeze agent comprising glycerol; and (e) biological buffer to provide the composition with a pH of about 6.9 to about 7.5, and wherein the composition comprises a sufficient amount of water so that the composition exhibits an osmolality of about 250 mOsM to about 350 mOsM, wherein the composition is substantially free of animal products. 2. An aqueous ready to use semen extender composition according to claim 1, wherein the composition comprises at least about 90 wt. % water. 3. An aqueous ready to use semen extender composition according to claim 1, further comprising antioxidant. 4. An aqueous ready to use semen extender composition according to claim 3, wherein the antioxidant comprises at least one of vitamin E, vitamin C, vitamin A, BHA, EHT, or derivatives thereof. 5. An aqueous ready to use semen extender composition according to claim 1, wherein the anionic surfactant comprises at least one of sodium lauryl sulfate, sodium laureth sulfate, or mixtures thereof. 6. An aqueous ready to use semen extender composition according to claim 1, further comprising semen. 7. A method for manufacturing an aqueous ready to use semen extender composition, the method comprising a step of: (a) mixing semen extender composition components to provide a semen extender composition having an osmolality of about 250 mOsM to about 350 mOsM and a pH of about 6.9 to about 7.5, the semen extender composition components comprising: (i) about 0.1 wt. % to about 6 wt. % phospholipid obtained from a non-animal source comprising lecithin; (ii) about 0.0001 wt. % to about 1 wt,% of anionic surfactant to reduce ice crystal formation during freezing of the composition, wherein the anionic surfactant comprises a sulfate; (iii) about 0.5 wt. % to about 3 wt.; (iv) about 3 wt. % to about 14 wt. % freeze agent comprising glycerol; (v) water; and (vi) biological buffer) wherein the composition is substantially free of animal products. 8. An aqueous ready to use semen extender composition according to claim 1, wherein the composition further comprises at least about 1 IU/ml antioxidant. 9. An aqueous ready to use semen extender composition according to claim 1, wherein the composition further comprises at least about 5 IU/ml antioxidant. 10. An aqueous ready to use semen extender composition according to claim 1, wherein the composition further comprises about 1 wt. % to about 3 wt. % antioxidant. 11. A method according to claim 7, wherein the composition components further comprise at least about 1 IU/ml antioxidant. 12. A method according to claim 7, wherein the composition components further comprise at least about 5 IU/ml antioxidant. 13. A method according to claim 7, wherein the composition components further comprise about 1 wt. % to about 3 wt. % antioxidant. 14. A method according to claim 11, wherein the antioxidant comprises at least one of vitamin E, vitamin C, vitamin A, BHA, BHT, or derivatives thereof. 15. A method according to claim 7, wherein the anionic surfactant comprises at least one of sodium lauryl sulfate, sodium laureth sulfate, or mixtures thereof.
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이 특허에 인용된 특허 (16)
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