Method of reducing the sensitivity of assay devices
원문보기
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
G01N-021/00
G01N-015/06
G01N-021/76
G01N-033/53
G01N-033/543
G01N-033/544
G01N-021/77
G01N-033/566
G01N-031/22
C12M-001/34
C12M-003/00
C12Q-001/00
출원번호
UP-0718996
(2003-11-21)
등록번호
US-7713748
(2010-06-03)
발명자
/ 주소
Wei, Ning
출원인 / 주소
Kimberly-Clark Worldwide, Inc.
대리인 / 주소
Dority & Manning, P.A.
인용정보
피인용 횟수 :
4인용 특허 :
330
초록▼
A flow-through assay device for detecting the presence or quantity of an analyte residing in a test sample is provided. The device utilizes a scavenging zone that contains a capture reagent for the analyte of interest. The capture reagent may capture a quantity of the analyte that is less than or eq
A flow-through assay device for detecting the presence or quantity of an analyte residing in a test sample is provided. The device utilizes a scavenging zone that contains a capture reagent for the analyte of interest. The capture reagent may capture a quantity of the analyte that is less than or equal to a predefined base quantity of the analyte, such as a quantity considered “normal” for a particular test sample. Thus, the capture reagent is able to prevent some of the analyte from being detected. In this manner, the sensitivity of the assay device may be reduced in a simple, inexpensive, yet effective manner.
대표청구항▼
What is claimed is: 1. A method for detecting an analyte in a test sample, the method comprising: i) providing an assay device that comprises: a sampling pad that defines a scavenging zone in which is non-diffusively immobilized a first capture reagent configured to specifically bind with the analy
What is claimed is: 1. A method for detecting an analyte in a test sample, the method comprising: i) providing an assay device that comprises: a sampling pad that defines a scavenging zone in which is non-diffusively immobilized a first capture reagent configured to specifically bind with the analyte; a conjugate pad that contains detection probes and optional calibration probes, the detection probes being conjugated with a first binding member configured to specifically bind with the analyte; and a porous membrane in fluid communication with the sampling pad and the conjugate pad, the porous membrane defining a detection zone in which is immobilized a second capture reagent configured to specifically bind with the analyte and a calibration zone within which is immobilized a third capture reagent including a polyelectrolyte having a net charge opposite to that of and configured to bind with the detection probes, the calibration probes, or combinations thereof, wherein the detection zone and the calibration zone are located downstream from the sampling pad and the conjugate pad; ii) contacting the assay device with the test sample, wherein a quantity of the analyte in the test sample less than or equal to a predefined base quantity binds to the first capture reagent at the scavenging zone and a quantity of the analyte in excess of the predefined base quantity binds to the specific binding member of the detection probes to form complexes that flow through the porous membrane and bind to the second capture reagent in the detection zone to generate a detection signal, and wherein the detection probes, the calibration probes, or a combination thereof, flow through the porous membrane and bind to the third capture reagent at the calibration zone to generate a calibration signal; iii) detecting the intensity of the detection signal and the calibration signal; and iv) comparing the intensity of the detection signal to the intensity of the calibration signal, wherein the quantity of the analyte within the test sample in excess of the predefined base quantity is proportional to the intensity of the detection signal calibrated by the intensity of the calibration signal. 2. A method as defined in claim 1, wherein said first capture reagent at said scavenging zone is selected from the group consisting of antigens, haptens, protein A or G, neutravidin, avidin, streptavidin, captavidin, antibodies, and complexes thereof. 3. A method as defined in claim 1, wherein said second capture reagent at said detection zone is selected from the group consisting of antigens, haptens, protein A or G, neutravidin, avidin, streptavidin, captavidin, antibodies, and complexes thereof. 4. A method as defined in claim 1, wherein said capture reagents at said scavenging zone and said detection zone are substantially identical. 5. A method as defined in claim 1, wherein said detection probes comprise a substance selected from the group consisting of chromogens, catalysts, luminescent compounds, radioactive compounds, direct visual labels, liposomes, and combinations thereof. 6. A method as defined in claim 1, wherein the conjugate pad is located downstream from the sampling pad. 7. A method as defined in claim 1, wherein the analyte includes an antigen. 8. A method as defined in claim 7, wherein the specific binding member of the detection probes includes an antibody. 9. A method as defined in claim 8, wherein the first capture reagent and the second capture reagent include antibodies that bind to the same epitope of the analyte. 10. A method as defined in claim 7, wherein the antigen includes C-reactive protein. 11. A method as defined in claim 1, wherein the test sample is blood or derived from blood. 12. A method as defined in claim 11, wherein the analyte includes C-reactive protein. 13. A method as defined in claim 12, wherein the pre-defined base quantity is about 10 micrograms of C-reactive protein per milliliter of the test sample. 14. A method as defined in claim 1, wherein the assay device further comprises a wicking pad in fluid communication with the porous membrane and located downstream from the detection zone and the calibration zone. 15. A method as defined in claim 1, wherein said polyelectrolyte has a net positive charge. 16. A method as defined in claim 15, wherein said polyelectrolyte is selected from the group consisting of polylysine, polyethyleneimine, epichlorohydrin-functionalized polyamines or polyamidoamines, polydiallyldimethyl-ammonium chloride, cationic cellulose derivatives, and combinations thereof. 17. A method as defined in claim 1, wherein said polyelectrolyte has a net negative charge. 18. A method as defined in claim 1, wherein the detection probes comprise latex microparticles.
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이 특허에 인용된 특허 (330)
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