Methods and kits for diagnosing or monitoring autoimmune and chronic inflammatory disease
원문보기
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
C12Q-001/68
C12P-019/34
출원번호
UP-0850897
(2007-09-06)
등록번호
US-7723037
(2010-06-14)
발명자
/ 주소
Richardson, Bruce
Lu, Qianjin
출원인 / 주소
The Regents of the University of Michigan
The United States of America as represented by the Department of Veterans Affairs
대리인 / 주소
Casimir Jones S.C.
인용정보
피인용 횟수 :
0인용 특허 :
27
초록▼
The present invention relates to compositions and methods for diagnosing, monitoring and/or treating an autoimmune or chronic inflammatory disease. In particular, the present invention provides methods for diagnosing, monitoring and treating an autoimmune disease (e.g., rheumatoid arthritis) or chro
The present invention relates to compositions and methods for diagnosing, monitoring and/or treating an autoimmune or chronic inflammatory disease. In particular, the present invention provides methods for diagnosing, monitoring and treating an autoimmune disease (e.g., rheumatoid arthritis) or chronic inflammatory disease (e.g., systemic lupus erythematosus) based on detecting or altering (e.g., altering expression or methylation status of) autoimmune or chronic inflammatory disease proteins (e.g., CD70 and CD40L). The present invention also provides kits for detecting methylation status of autoimmune or chronic inflammatory disease proteins (e.g., CD70 and CD40L) and for diagnosing, monitoring and/or treating autoimmune or chronic inflammatory diseases.
대표청구항▼
We claim: 1. A method for detecting methylation status of CD70 in a subject, comprising: a) providing a biological sample from said subject, wherein said biological sample comprises CD70 nucleic acid; b) exposing said sample to reagents for detecting methylation status of CD70 nucleic acid; c) dete
We claim: 1. A method for detecting methylation status of CD70 in a subject, comprising: a) providing a biological sample from said subject, wherein said biological sample comprises CD70 nucleic acid; b) exposing said sample to reagents for detecting methylation status of CD70 nucleic acid; c) detecting methylation status of CD70 nucleic acid in said sample, and d) comparing said methylation status of CD70 nucleic acid in said sample to the methylation status of CD70 nucleic acid in a control sample, wherein said control sample is from a subject without rheumatoid arthritis or systemic lupus erythematosis, and wherein said methylation status is correlated with the presence or absence of rheumatoid arthritis or systemic lupus erythematosis in said subject. 2. The method of claim 1, wherein said reagents detect methylation status of the 5′ untranslated region of CD70. 3. The method of claim 2, wherein said 5′ untranslated region comprises the −338 to −515 region of CD70. 4. The method of claim 2, wherein said 5′ untranslated region comprises the −466 to −515 region of CD70. 5. The method of claim 2, wherein said 5′ untranslated region comprises the −338 to −466 region of CD70. 6. The method of claim 1, wherein said biological sample is selected from the group consisting of bone marrow, whole blood, serum, plasma, interstitial fluid, urine, cerebrospinal fluid, and tissue. 7. The method of claim 1, wherein said nucleic acid is DNA. 8. The method of claim 1, wherein said detecting comprises use of a polymerase chain reaction. 9. The method of claim 8, wherein said polymerase chain reaction is methylation sensitive. 10. The method of claim 1, wherein said detecting comprises differential antibody binding, oligonucleotide binding assays, or use of a microarray. 11. The method of claim 1, wherein said detecting comprises restriction enzyme digestion. 12. The method of claim 1, wherein said detecting comprises using a kit comprising reagents sufficient for detecting methylation status of CD70 in a subject. 13. The method of claim 12, wherein said kit comprises a positive control that indicates CD70 methylation status. 14. The method of claim 1, further comprising detecting methylation status of one or more of CD40L, perform CD11a, CD11c, IgE, FCRγ1, and CD30.
연구과제 타임라인
LOADING...
LOADING...
LOADING...
LOADING...
LOADING...
이 특허에 인용된 특허 (27)
Nayak P. Narayan (Yarmouth ME), Apparatus and method for performing immunoassays.
Babson Arthur L. ; Palmieri Thomas ; Montalbano Anthony P. ; Montalbano Chris P. ; Montalbano Greg A. ; Fleischer Eric C., Automated immunoassay analyzer.
Brow Mary Ann D. ; Hall Jeff Steven Grotelueschen ; Lyamichev Victor ; Olive David Michael ; Prudent James Robert, Detection of nucleic acid sequences by invader-directed cleavage.
Brow Mary Ann D. ; Hall Jeff Steven Grotelueschen ; Lyamichev Victor ; Olive David Michael ; Prudent James Robert, Detection of nucleic acid sequences by invader-directed cleavage.
Dowell Barry L. (Mundelein IL) Alexander Debra B. (Gurnee IL) O\Morchoe Susan B. (Woodridge IL) King Carol A. (Highland Park IL) Smith Allan H. (Zion IL), Immunoassays for prostate specific antigen.
Dowell Barry L. (Mundelein IL) King Carol A. (Highland Park IL) Alexander Debra B. (Gurnee IL) Smith Allan H. (Zion IL) O\Morchoe Susan B. (Woodridge IL), Immunoassays for prostate specific antigen.
Livak Kenneth J. (San Jose CA) Flood Susan J. A. (Fremont CA) Marmaro Jeffrey (Foster City CA), Method for detecting nucleic acid amplification using self-quenching fluorescence probe.
Willey James C. ; Crawford Erin L. ; DeMuth Jeffery P. ; Jackson Clara M. ; Weaver David A., Method for quantitative measurement of gene expression using multiplex competitive reverse transcriptase-polymerase cha.
Willey James C. (Rochester NY), Method for quantitative measurement of gene expression using multiplex competitive reverse transcriptase-polymerase chai.
Willey James C. (Rochester NY), Method for quantitative measurement of gene expression using multiplex competitive reverse transcriptase-polymerase chai.
Herman James G. ; Baylin Stephen B., Method of detection of methylated nucleic acid using agents which modify unmethylated cytosine and distinguish modified methylated and non-methylated nucleic acids.
Herman James G. ; Baylin Stephen B., Method of detection of methylated nucleic acid using agents which modify unmethylated cytosine and distinguishing modifi.
Herman James G. ; Baylin Stephen B., Method of detection of methylated nucleic acid using agents which modify unmethylated cytosine and distinguishing modified methylated and non-methylated nucleic acids.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.