Low volume filtration column devices and methods of filtering therewith
원문보기
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
C07H-021/00
B01D-015/08
C02F-001/28
출원번호
UP-0209508
(2002-07-30)
등록번호
US-7749388
(2010-07-26)
발명자
/ 주소
Pai, Derek S.
Kunitake, Steven T.
Richardson, Derrick
Monteon, Jorge
출원인 / 주소
Life Technologies Corporation
대리인 / 주소
Fish & Richardson P.C.
인용정보
피인용 횟수 :
2인용 특허 :
50
초록▼
This relates to filter columns for isolating nucleic acids, particularly at small elution volumes. The filter column is adapted for stable placement within the upper portion of standard plastic collection tubes of various sizes. The body of the filter column has a number of surfaces to accommodate p
This relates to filter columns for isolating nucleic acids, particularly at small elution volumes. The filter column is adapted for stable placement within the upper portion of standard plastic collection tubes of various sizes. The body of the filter column has a number of surfaces to accommodate placement within variously sized collection tubes. The filter column contains nucleic acid-specific filter which can be located at alternate regions within the filter column, providing different filter surface areas and loading volume capacities using the same column body. The filter column has an opening on an upper end adapted to be sealed by a cap. A method for recovering nucleic acids using such filter column is also provided.
대표청구항▼
We claim: 1. A filter column, comprising: a body having a passageway extending therethrough, the body having a first end, a second end and a longitudinal axis, an outer surface and an inner surface, a first body portion proximate to the first end, a third body portion proximate to the second end an
We claim: 1. A filter column, comprising: a body having a passageway extending therethrough, the body having a first end, a second end and a longitudinal axis, an outer surface and an inner surface, a first body portion proximate to the first end, a third body portion proximate to the second end and configured to receive and retain a filter assembly comprising a retainer, a filter and a filter support, and a second body portion disposed between the first and third body portions; the outer surface having at least one bearing surface for seating on at least one collection tube; wherein the inner surface comprises at least one angled surface located proximate or above the at least one bearing surface that forms an angle with the longitudinal axis that is less than approximately 90 degrees; a filter disposed within the third body portion, the filter comprising a first side proximal to the first end and a second side proximal to the second end and defining a perimeter; a filter support disposed within the third body portion and contacting the second side of the filter and supporting the filter; and a retainer disposed within the third body portion and contacting the first side of the filter; the retainer having an outer edge, an inner edge, and an upper surface. 2. The filter column of claim 1 wherein the outer surface further includes a second bearing surface for seating on at least a second collection tube that has a different size from the at least one collection tube. 3. The filter column of claim 1 wherein at least one bearing surface is located between the first body portion and the second body portion. 4. The filter column of claim 1 wherein at least one angled surface is located between the first body portion and the second body portion. 5. The filter column of claim 1 wherein the second body portion is configured to be removably locatable within a collection tube having a volume of approximately 1.5 to 2.0 milliliters. 6. The filter column of claim 1 wherein the cross-sectional area of the passageway at the first body portion is greater than the cross-sectional area of the passageway at second body portion. 7. The filter column of claim 1 wherein at least one bearing surface is located between the second body portion and the third body portion. 8. The filter column of claim 1 wherein at least one angled surface is located between the second body portion and the third body portion. 9. The filter column of claim 1 wherein the third body portion is configured to be removably locatable within a collection tube having a volume of approximately 0.5 milliliters. 10. The filter column of claim 1 wherein the passageway at the third body portion has a cross-sectional area; the cross-sectional area of the passageway at the second body portion being greater than the cross-sectional area of the passageway at the third body portion. 11. The filter column of claim 1 wherein the filter support is a porous membrane. 12. The filter column of claim 11 wherein the filter support has a substantially smooth upper surface. 13. The filter column of claim 1 further including a lid adapted to mate with the first end of the body. 14. The filter column of claim 13 wherein at least one vent is defined in the body at the first end. 15. The filter column of claim 1 wherein the inner surface is configured such that fluid disposed into the filter column does not pool on the angled surface while stationary or while undergoing centrifugation. 16. The filter column claim 1 wherein the at least one angled surface forms an angle with the longitudinal axis that is approximately 45 degrees or less. 17. The filter column of claim 1 wherein each of the bearing surfaces is adapted to seat on respective collection tubes of different sizes. 18. The filter column of claim 1 wherein the first end of the body is adapted to mate with a sample carrier. 19. The filter column of claim 1 wherein the upper surface of the retainer is beveled such that the upper surface forms an angle with the longitudinal axis of less than approximately 90 degrees. 20. The filter column of claim 1 wherein the upper surface of the retainer is beveled such that fluid disposed into the filter column does not pool on the upper surface while stationary or while undergoing centrifugation. 21. The filter column of claim 20 wherein the retainer has a lower surface that is beveled. 22. The filter column of claim 21 wherein the upper and lower surfaces of the retainer are beveled such that the thickness of the retainer decreases with distance from the outer edge towards the inner edge. 23. The filter column of claim 1 wherein at least a portion of the filter is compressed by the retainer. 24. The filter column of claim 23 wherein the at least a portion of the filter that is compressed by the retainer is the outer edge of the filter. 25. The filter column of claim 1 wherein the retainer is adapted to compress the filter such that at least a portion of the filter proximate to the perimeter is compressed. 26. The filter column of claim 1 wherein the outer surface has at least two bearing surfaces for seating on collection vessels of at least two different sizes. 27. The filter column of claim 1 wherein the retainer is ring-shaped. 28. A filter column, comprising: a body having a passageway extending therethrough, the body having, an outer surface, an inner surface, and a longitudinal axis; and a filter disposed within the passageway, the filter having a perimeter; wherein at least a portion of the filter has a greater density relative to another portion of the filter and the filter includes a compression profile such that the compression of the filter gradually increases with distance towards the outer edge. 29. A method for isolating nucleic acid material, comprising: providing a filter column; the filter column comprising: (a) a body having a passageway extending therethrough, the body having a first end, a second end and a longitudinal axis, an outer surface and an inner surface, a first body portion proximate to the first end, a third body portion proximate to the second end and configured to receive and retain a filter assembly comprising a retainer, a filter and a filter support, and a second body portion disposed between the first and third body portions; the outer surface having at least one bearing surface for seating on at least one collection tube; wherein the inner surface comprises at least one angled surface located proximate or above the at least one bearing surface that forms an angle with the longitudinal axis that is less than approximately 90 degrees; (b) a filter disposed within the third body portion, the filter comprising a first side proximal to the first end and a second side proximal to the second end and defining a perimeter and a wetting capacity; (c) a filter support disposed within the third body portion and contacting the second side of the filter and supporting the filter; and (d) a retainer disposed within the third body portion and contacting the first side of the filter; the retainer having an outer edge, an inner edge, and an upper surface providing a solution containing nucleic acid material; transferring the solution containing nucleic acid to the filter of the filter column; transferring solution from the filter column; adding elution buffer to the filter in an amount substantially equal to the wetting capacity of the filter; and eluting the nucleic acid from the filter column. 30. The method of claim 29 wherein the step of transferring the solution containing nucleic acid to the filter of the filter column further includes transfer the solution containing nucleic acid to the filter of the filter column in the presence of an agent capable of promoting binding of the nucleic acid material to the filter. 31. The method of claim 29 further including the step of washing the filter column with a wash solution. 32. The method of claim 29 further including the step of adding additional elution buffer to the filter column in an amount that is between zero and approximately fifty percent of the wetting capacity of the filter. 33. The method of claim 29 further including the step of adding additional elution buffer to the filter column in an amount that is between zero and approximately 66 percent. 34. A method for isolating a nucleic acid material, comprising: providing a filter column; the filter column comprising: (a) a body having a passageway extending therethrough, the body having a first end, a second end and a longitudinal axis, an outer surface and an inner surface, a first body portion proximate to the first end, a third body portion proximate to the second end and configured to receive and retain a filter assembly comprising a retainer, a filter and a filter support, and a second body portion disposed between the first and third body portions; the outer surface having at least one bearing surface for seating on at least one collection tube; wherein the inner surface comprises at least one angled surface located proximate or above the at least one bearing surface that forms an angle with the longitudinal axis that is less than approximately 90 degrees; (b) a filter disposed within the third body portion, the filter comprising a first side proximal to the first end and a second side proximal to the second end and defining a perimeter and having a shape that defines a volume; (c) a filter support disposed within the third body portion and contacting the second side of the filter and supporting the filter; and (d) a retainer disposed within the third body portion and contacting the first side of the filter; the retainer having an outer edge, an inner edge, and an upper surface; providing a solution containing nucleic acid material; transferring the solution containing nucleic acid to the filter; transferring the solution from the filter column; adding elution buffer to the filter column in an amount substantially equal to the volume defined by the shape of the filter; and eluting the nucleic acid from the filter column. 35. The method of claim 34 wherein the step of transferring the solution containing nucleic acid to the filter further includes transferring the solution containing nucleic acid to the filter in the presence of an agent capable of promoting binding of the nucleic acid material to the filter. 36. The method of claim 34 further including the step of washing the filter column with a wash solution. 37. A method for isolating a nucleic acid material comprising: providing a filter column; the filter column comprising: (a) a body having a passageway extending therethrough, the body having a first end, a second end and a longitudinal axis, an outer surface and an inner surface, a first body portion proximate to the first end, a third body portion proximate to the second end and configured to receive and retain a filter assembly comprising a retainer, a filter and a filter support, and a second body portion disposed between the first and third body portions; the outer surface having at least one bearing surface for seating on at least one collection tube; wherein the inner surface comprises at least one angled surface located proximate or above the at least one bearing surface that forms an angle with the longitudinal axis that is less than approximately 90 degrees; (b) a filter disposed within the third body portion, the filter comprising a first side proximal to the first end and a second side proximal to the second end and defining a perimeter and a wetting capacity; (c) a filter support disposed within the third body portion and contacting the second side of the filter and supporting the filter; and (d) a retainer disposed within the third body portion and contacting the first side of the filter; the retainer having an outer edge, an inner edge, and an upper surface providing solution containing nucleic acid material in the filter column; locating the filter column in fluid communication with the at least one collection tube; transferring the solution containing nucleic acid to the filter of the filter column; transferring the solution from the filter column at a first speed followed by a second speed wherein the first speed is slower than the second speed; adding elution buffer into the filter column; and eluting the nucleic acid material from the filter column. 38. The method of claim 37 wherein the step of transferring the solution containing nucleic acid to the filter further includes transferring the solution containing nucleic acid to the filter in the presence of an agent capable of promoting binding of the nucleic acid material to the filter. 39. The method of claim 37 wherein the first speed is approximately 500 g or less. 40. The method of claim 37 wherein the first speed is approximately 1000 g or less. 41. The method of claim 37 wherein the first speed is approximately 1500 g or less. 42. The method of claim 37 wherein the first speed is approximately 2000 g or less. 43. The method as in one of claims 37-42 wherein the second speed is approximately 10,000 g or more. 44. The method of claim 37 the step of transferring the solution from the filter column is performed via centrifugal force. 45. The method of claim 37 wherein the outer surface of the filter column further includes a second bearing surface for seating at least a second collection tube that has a different size from the at least one collection tube; and wherein the step of transferring the solution containing nucleic acid from the filter column includes transferring the solution containing nucleic acid to the at least one collection tube. 46. The method of claim 37 wherein the step of adding elution buffer includes adding elution buffer in an amount that is substantially equal to the wetting capacity of the filter. 47. The method of claim 45 further including the steps of: removing the filter column from the at least one collection tube; seating the filter column on a the second collection tube; and wherein the step of eluting the nucleic acid material from the filter column includes eluting the nucleic acid material into the second collection tube. 48. The method of claim 47 wherein the second collection tube is smaller than the at least one collection tube. 49. The method of claim 37 further including the step of washing the filter with a wash solution. 50. The method of claim 37 wherein the step of eluting the nucleic acid material from the filter column is performed a first speed followed by a second speed wherein the first speed is slower than the second speed. 51. The method of claim 50 wherein the first speed of the elution step is approximately 500 g or less. 52. The method of claim 50 wherein the first speed of the elution step is approximately 1000 g or less. 53. The method of claim 50 wherein the first speed of the elution step is approximately 1500 g or less. 54. The method of claim 50 wherein the first speed of the elution step is approximately 2000 g or less. 55. The method as in one of claims 50-54 wherein the second speed of the elution step is approximately 10,000 g or more. 56. The method of claim 37, wherein the solution containing a nucleic acid material consists of a lysate solution of prepared from biological material disrupted with a disruption solution. 57. A method for isolating a nucleic acid material comprising: providing a filter column; the filter column comprising: (a) a body having a passageway extending therethrough, the body having a first end, a second end and a longitudinal axis, an outer surface and an inner surface, a first body portion proximate to the first end, a third body portion proximate to the second end and configured to receive and retain a filter assembly comprising a retainer, a filter and a filter support, and a second body portion disposed between the first and third body portions; the outer surface having at least one bearing surface for seating on at least one collection tube; wherein the inner surface comprises at least one angled surface located proximate or above the at least one bearing surface that forms an angle with the longitudinal axis that is less than approximately 90 degrees; (b) a filter disposed within the third body portion, the filter comprising a first side proximal to the first end and a second side proximal to the second end and defining a perimeter; (c) a filter support disposed within the third body portion and contacting the second side of the filter and supporting the filter; and (d) a retainer disposed within the third body portion and contacting the first side of the filter; the retainer having an outer edge, an inner edge, and an upper surface; providing solution containing nucleic acid material in the filter column; locating the filter column in fluid communication with the at least one collection tube; transferring the solution containing nucleic acid to the filter of the filter column; transferring solution from the filter column; adding elution buffer into the filter column; and eluting the nucleic acid material from the filter column at a first speed followed by a second speed wherein the first speed is slower than the second speed. 58. The method of claim 57 wherein the step of transferring the solution containing nucleic acid to the filter of the filter column further includes transferring the solution containing nucleic acid to the filter of the filter column in the presence of an agent capable of promoting binding of the nucleic acid material to the filter. 59. The method of claim 57 wherein the first speed of the elution step is approximately 500 g or less. 60. The method of claim 57 wherein the first speed of the elution step is approximately 1000 g or less. 61. The method of claim 57 wherein the first speed of the elution step is approximately 1500 g or less. 62. The method of claim 57 wherein the first speed of the elution step is approximately 2000 g or less. 63. The method as in one of claims 57-62 wherein the second speed of the elution step is approximately 10,000 g or more. 64. The method of claim 57 wherein the step of transferring the solution from the filter column is performed via centrifugal force. 65. The method of claim 57 wherein the outer surface of the filter column further includes a second bearing surface for seating at least a second collection tube that has a different size from the at least one collection tube; and wherein the step of transferring the solution containing nucleic acid from the filter column includes transferring the solution containing nucleic acid to the at least one collection tube. 66. The method of claim 65 further including the steps of: removing the filter column from the at least one collection tube; locating the filter column in fluid communication with the second collection tube; and wherein the step of eluting the nucleic acid material from the filter column includes eluting the nucleic acid material into the second collection tube. 67. The method of claim 66 wherein the second collection tube is smaller than the first collection tube. 68. The method of claim 57 further including the step of washing the filter with a wash solution. 69. The method of claim 57 wherein the step of transferring solution from the filter is performed at a first speed followed by a second speed wherein the first speed is slower than the second speed. 70. The method of claim 69 wherein the first speed of the transfer step is approximately 500 g or less. 71. The method of claim 69 wherein the first speed of the transfer step is approximately 1000 g or less. 72. The method of claim 69 wherein the first speed of the transfer step is approximately 1500 g or less. 73. The method of claim 69 wherein the first speed of the transfer step is approximately 2000 g or less. 74. The method as in one of claims 69-73 wherein the second speed of the transfer step is approximately 10,000 g or more. 75. The method of claim 57, wherein the solution containing a nucleic acid material consists of a lysate solution of prepared from biological material disrupted with a disruption solution.
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이 특허에 인용된 특허 (50)
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