Instruments and methods for exposing a receptacle to multiple thermal zones
원문보기
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
C12M-001/00
C12M-003/00
출원번호
UP-0333484
(2008-12-12)
등록번호
US-7767447
(2010-08-24)
발명자
/ 주소
Breidenthal, Scott S.
Fan, Sara H.
Lee, Richard S.
Nelson, Norman C.
Poirier, Michael S.
Scott, Matthew J.
Taylor, Jason A.
출원인 / 주소
Gen-Probe Incorporated
Qualigen, Inc.
대리인 / 주소
Rothwell, Figg, Ernst & Manbeck, PC
인용정보
피인용 횟수 :
36인용 특허 :
272
초록▼
A receptacle having a plurality of interconnected chambers arranged to permit multiple process steps or processes to be performed independently or simultaneously. The receptacles are manufactured to separate liquid from dried reagents and to maintain the stability of the dried reagents. An immiscibl
A receptacle having a plurality of interconnected chambers arranged to permit multiple process steps or processes to be performed independently or simultaneously. The receptacles are manufactured to separate liquid from dried reagents and to maintain the stability of the dried reagents. An immiscible liquid, such as an oil, is included to control loading of process materials, facilitate mixing and reconstitution of dried reagents, limit evaporation, control heating of reaction materials, concentrate solid support materials to prevent clogging of fluid connections, provide minimum volumes for fluid transfers, and to prevent process materials from sticking to chamber surfaces. The receptacles can be adapted for use in systems having a processing instrument that includes an actuator system for selectively moving fluid substances between chambers and a detector. The actuator system can be arranged to concentrate an analyte present in a sample. The detector can be used to detect an optical signal emitted by the contents of the receptacle.
대표청구항▼
The invention claimed is: 1. An instrument for processing a sample in a receptacle having a plurality of interconnected chambers, the instrument being constructed and arranged to support the receptacle in an operative position during the processing and comprising: one or more thermal elements each
The invention claimed is: 1. An instrument for processing a sample in a receptacle having a plurality of interconnected chambers, the instrument being constructed and arranged to support the receptacle in an operative position during the processing and comprising: one or more thermal elements each defining a multiple chamber thermal zone disposed to be in thermal communication with the receptacle and constructed and arranged to transmit thermal energy between each multiple chamber thermal zone and an associated region of the receptacle, wherein the associated region of the receptacle encompasses all or a portion of each of two or more but less than all chambers of the receptacle; and a controller programmed to control operation of the one or more thermal elements defining the multiple chamber thermal zones to selectively heat or cool the chambers encompassed within the regions associated with the multiple chamber thermal zones. 2. The instrument of claim 1, further comprising one or more thermal elements defining one or more single chamber thermal zones disposed to be in thermal communication with the receptacle and constructed and arranged to transmit thermal energy between each single chamber thermal zone and an associated region of the receptacle encompassing all or a portion of one chamber of the receptacle, wherein the controller is programmed to control operation of the thermal elements defining the single chamber thermal zones to selectively heat or cool the chambers encompassed within the regions associated with the single chamber thermal zones. 3. The instrument of claim 1, wherein the thermal elements comprise one or more Peltier devices controlled by the controller to selectively heat or cool a body with which the Peltier' device is in thermal contact. 4. The instrument of claim 1, wherein the thermal elements comprise a heat transfer element formed from a thermally conductive material and having a peripheral shape corresponding to a predetermined shape of a multiple chamber thermal zone defined by the heat transfer element. 5. The instrument of claim 4, further comprising a temperature sensor in thermal communication with the heat transfer element and adapted to sense the temperature of the heat transfer element and communicate the sensed temperature to the controller. 6. The instrument of claim 4, wherein the heat transfer element is held within the instrument in a fixed position with respect to the receptacle. 7. The instrument of claim 4, wherein the heat transfer element is formed from aluminum. 8. The instrument of claim 1, wherein the thermal elements comprise: one or more Peltier devices controlled by the controller to selectively heat or cool a body with which the Peltier devices are in thermal contact; and a heat transfer element associated with each multiple chamber thermal zone, said heat transfer element being formed from a thermally conductive material and having a generally flat surface and a peripheral shape corresponding to a predetermined shape of a multiple chamber thermal zone defined by the heat transfer element, wherein the Peltier devices are in thermal contact with the heat transfer element. 9. The instrument of claim 4, wherein the instrument comprises multiple thermal elements, each thermal element being separated from other thermal elements by isolating structure comprising a thermally non-conductive material. 10. The instrument of claim 9, wherein the heat transfer element and the isolating structure are held within the instrument in fixed positions with respect to the receptacle. 11. The instrument of claim 3, further comprising a heat dissipation element constructed and arranged to dissipate heat from the Peltier devices. 12. The instrument of claim 11, wherein the heat dissipation element comprises a heat sink formed from a conductive material and including a block having one side in thermal communication with at least one Peltier device and an opposite side from which heat dissipation fins extend from the block. 13. The instrument of claim 12, wherein the heat dissipation element further comprises a fan disposed adjacent the heat sink and configured to generate an air flow over the heat dissipation fins of the heat sink. 14. The instrument of claim 13, wherein operation of the fan is controlled by the controller. 15. The instrument of claim 12, wherein the heat sink is formed from aluminum. 16. The instrument of claim 1, further comprising one or more temperature sensors for sensing the temperature of each thermal zone and communicating the sensed temperature to the controller. 17. The instrument of claim 1, wherein the controller is configured to control the operation of the thermal elements to establish an ambient temperature within a prescribed temperature range. 18. The instrument of claim 17, wherein the prescribed temperature range is about 20° C. to 40° C. 19. The instrument of claim 17, wherein the prescribed temperature range is about 25° C. to 37° C. 20. The instrument of claim 1, wherein the controller is configured to control operation of the thermal elements to heat one or more chambers to temperatures within a prescribed temperature range. 21. The instrument of claim 20, wherein the prescribed temperature range encompasses temperatures required to perform a process requiring thermal cycling. 22. The instrument of claim 21, wherein the prescribed temperature range is about 5° C. to 95° C. 23. The instrument of claim 21, wherein the process is a PCR amplification reaction. 24. The instrument of claim 1, wherein the controller is configured to control operation of the thermal elements to heat or cool the contents of chambers encompassed within a region associated with the multiple chamber thermal zone to a predetermined temperature for a predetermined period of time. 25. The instrument of claim 1, wherein the one or more thermal elements define at least two multiple chamber thermal zones. 26. The instrument of claim 1, wherein when a receptacle is supported in the operative position within the instrument, filling a chamber encompassed within the region associated with the multiple chamber thermal zone with fluid will increase thermal communication between the chamber and the multiple chamber thermal zone. 27. A method for heating or cooling substances within a receptacle having a plurality of interconnected chambers, the method comprising the steps of: positioning the receptacle in thermal communication with one or more multiple chamber thermal zones contained in an analyzer, each multiple chamber thermal zone being associated with a region of the receptacle encompassing all or a portion of each of two or more but less than all chambers of the receptacle; and transmitting thermal energy between each multiple chamber thermal zone and the chambers encompassed by the region associated with the multiple chamber thermal zone to selectively heat or cool substances contained within the encompassed chambers to a temperature different than the temperature of the chambers encompassed by at least one other region. 28. The method of claim 27, further comprising positioning the receptacle in thermal communication with one or more single chamber thermal zones contained in the analyzer, each single chamber thermal zone being associated with a region of the receptacle encompassing all or a portion of one chamber of the receptacle. 29. The method of claim 27, wherein the transmitting step comprises heating or cooling the multiple chamber thermal zone with one or more Peltier devices. 30. The method of claim 27, wherein the ambient temperature of the analyzer is different than the temperature of substances contained within chambers encompassed by the regions of the receptacle associated with each multiple chamber thermal zone during the method. 31. The method of claim 27, wherein the transmitting step comprises alternately heating and cooling at least one of the multiple chamber thermal zones. 32. The method of claim 27, further comprising thermally separating each multiple chamber thermal zone from other multiple chamber thermal zones. 33. The method of claim 27, further comprising dissipating heat from the multiple chamber thermal zone. 34. The method of claim 27, further comprising sensing the temperature of each multiple chamber thermal zone. 35. The method of claim 27, further comprising expanding a chamber encompassed by the region of the receptacle associated with a multiple chamber thermal zone to increase the thermal communication between the expanded chamber and the associated multiple chamber thermal zone. 36. The method of claim 27, wherein the analyzer contains at least three multiple chamber thermal zones. 37. The method of claim 27, wherein each of the plurality of interconnected chambers is adjacent to at least one other chamber of the receptacle. 38. The method of claim 27, wherein each of the chambers of the receptacle comprises a flexible portion to facilitate substance movement between chambers. 39. The method of claim 38, wherein the receptacle comprises opposed members, at least one of which members comprises a flexible sheet. 40. The method of claim 39, wherein each of the opposed members comprises a flexible sheet. 41. The method of claim 29, wherein the heat is transferred to/from the multiple chamber thermal zone from/to the Peltier device using a thermal element. 42. The method of claim 41, wherein the thermal element comprises a metal plate having a size and shape encompassing all or a portion of each of two or more but less than all chambers of the receptacle. 43. The instrument of claim 1, wherein the each of the one or more thermal elements comprises a metal plate having a size and shape encompassing all or a portion of each of two or more but less than all chambers of the receptacle.
연구과제 타임라인
LOADING...
LOADING...
LOADING...
LOADING...
LOADING...
이 특허에 인용된 특허 (272)
Gabrielson Donn E., 1D finder pattern for 2D bar codes.
Birkenmeyer Larry G. (Chicago IL) Carrino John J. (Gurnee IL) Dille Bruce J. (Antioch IL) Hu Hsiang-Yun (Libertyville IL) Kratochvil Jon D. (Kenosha WI) Laffler Thomas G. (Libertyville IL) Marshall R, Amplification of target nucleic acids using gap filling ligase chain reaction.
Subramanian Kumar (Alameda CA) Sugarman Jeffrey (Sunnyvale CA) Sterling Bernhard B. (Danville CA) Voss Fred (Pleasanton CA) Saltman Marina (Redwood City CA), Analytical cartridge and system for detecting analytes in liquid samples.
Zaun Peter (Libertyville IL) Bouma Stanley R. (Grayslake IL) Gordon Julian (Lake Bluff IL) Kotlarik John J. (Vernon Hills IL), Apparatus and method for amplifying and detecting target nucleic acids.
Johnson Larry J. (San Jose CA) Widunas Joseph T. (Berkeley CA), Apparatus and method for performing automated amplification of nucleic acid sequences and assays using heating and cooli.
Mullis Kary B. (La Jolla CA) Johnson Larry (San Jose CA) Leath Richard A. (Berkley CA) Wennberg Timothy J. (Mariposa CA) Mezei Louis M. (Madison WI) Widunas Joseph T. (Freemont CA), Apparatus and method for performing automated amplification of nucleic acid sequences and assays using heating and cooli.
Caprio Craig A. (Rochester NY) Van der Gaag Michael R. (Rochester NY) Hinckley Charles C. (Santa Rosa CA) Chemelli John B. (Webster NY), Apparatus for heating a fluid-carrying compartment of reaction cuvette.
Miriam Lisa Ronchi ; David J. Regester ; Robert Kenneth Kobos ; Raymond E. Jackson, Jr., Apparatus for integrated polymerase chain reaction and capillary electrophoresis.
Kristen L. Manion (2600 San Leandro Blvd. ; Apt. 1508 San Leandro CA 94578) Wardlaw Stephen C. (191 N. Cove Rd. Old Saybrook CT 06475) Levine Robert A. (31 Pilgrim La. Guilford CT 06437), Apparatus for measuring blood constituent counts.
Zander Dennis R. (Penfield NY) Chemelli John B. (Rochester NY) Caprio Craig A. (Rochester NY), Automatic sealing closure means for closing off a passage in a flexible cuvette.
McNeil Mark D. (Irvine CA) Goldstein Robert H. (Tustin CA) Stahl Robert M. (Indianapolis IN) Piepho Sandra L. (Indianapolis IN), Blood analyzer system.
Hogan James J. (Coronado CA) Arnold ; Jr. Lyle J. (San Diego CA) Nelson Norman C. (San Diego CA) Bezverkov Robert (Cardiff by the Sea CA), Branched nucleic acid probes.
Hillman Robert S. (Cupertino CA) Cobb Michael E. (Sunnyvale CA) Allen Jim (Los Altos CA) Gibbons Ian (Menlo Park CA) Ostoich Vladimir (San Jose CA) Winfrey Laura J. (Belmont CA), Capillary flow device.
Hillman Robert S. (Cupertino CA) Cobb Michael E. (Sunnyvale CA) Allen Jimmy D. (Los Altos CA) Gibbons Ian (Menlo Park CA) Ostoich Vladimir E. (San Jose CA) Winfrey Laura J. (Belmont CA), Capillary flow device.
Shartle Robert (Livermore CA) Besemer Donald (Los Altos Hills CA) Gorin Michael (Los Altos CA), Capillary stop-flow junction having improved stability against accidental fluid flow.
Curtis Huntington W. (Chelsea NY) Kellogg Robert M. (Washington Crossing PA) Kissinger Kerry W. (Pennington NJ) Mappes Robert P. (Cranbury NJ) Stephans Emery J. (Plainsboro NJ), Chemical analysis system including a test package and rotor combination.
Mahan Donald E. (Grafton MA) Kearney Kevin R. (Worcester MA) Shimei Thomas M. (Franklin MA) Bate Ernest (Great Chart GB2) Missing Philip (Egerton GB2) Robinson David (Folkestone GB2), Closed disposable package.
Bloch Will (San Mateo CA) Raymond Jonathan C. (Orinda CA) Read Alan R. (Belmont CA), Compositions and methods for inhibiting dimerization of primers during storage of polymerase chain reaction reagents.
Nelson Norman Charles (San Diego CA) Woodhead James Stuart (Raglan GB3) Weeks Ian (Cardiff GB3) Cheikh Azzouz Ben (Del Mar CA), Compositions and methods for the simultaneous detection and quantification of multiple specific nucleic acid sequences.
Schnipelsky Paul N. (Rochester NY) Seaberg Leonard J. (Penfield NY) Hinckley Charles C. (Pittsford NY) Wellman Jeffrey A. (Rochester NY) Donish William H. (Rochester NY) Findlay John B. (Rochester NY, Containment cuvette for PCR and method of use.
Schnipelsky, Paul Nicholas; Seaberg, Leonard Joseph; Hinckley, Charles Cullis; Wellman, Jeffrey Allen; Donish, William Harold; Findlay, John Bruce, Containment cuvette for PCR and method of use.
Murphy Harold R. (209 N. Bellinger St. Herkimer NY 13350) DuBois Jeffrey A. (R.D. #1 Box 424 Wynn Rd. Holland Patent NY 13350) Strickland Reid A. (R.D. #1 Box 424 AA Wynn Rd. Holland Patent NY 13350), Cuvette for automated testing machine.
Woodhead James Stuart (Cardiff GBX) Weeks Ian (Cardiff GBX) Batmanghelich Shariar (Cardiff GBX), Detecting or quantifying multiple analytes using labelling techniques.
Bouma Stanley R. (Grayslake IL) Coules Ronald A. (Barrington IL) Gordon Julian (Lake Bluff IL) Shain Eric B. (Glencoe IL) Solomon Natalie A. (Buffalo Grove IL) Zaun Peter (Libertyville IL), Device and method for amplifying and detecting target nucleic acids.
Corbett John M. (Drummoyne AUX) Reed Kenneth C. (Monash CA AUX) Riggs Arthur D. (La Verne CA), Device and method for the automated cycling of solutions between two or more temperatures.
Blumenfeld Martin ; Bar-Cohen Avram ; Cibuzar Gregory T. ; Schiller Peter ; Arik Mehmet, Device and method to directly control the temperature of microscope slides.
Green Nancy F. (Pittsford NY) Cummins Thomas J. (Rochester NY) Oakes Fred T. (Melvern PA), Device for moving a target-bearing solid through a liquid for detection while being contained.
Woudenberg Timothy W. ; Albin Michael ; Kowallis Reid B. ; Raysberg Yefim ; Ragusa Robert P. ; Winn-Deen Emily S., Device for multiple analyte detection.
Guigan Jean (5 ; rue des Ursulines 75005 Paris FRX), Device for performing biological analyses by immunoenzymatic detection of antibodies or antigens in a serum.
Robinson David (Folkestone GB2) Bate Ernest (Great Chart GB2) Kellard Simon (Ashford GB2) Watson Mark (Ashford MA GB2) Mahan Donald E. (Grafton MA) Shimei Thomas M. (Franklin MA) Kearney Kevin R. (Wo, Diagnostics instrument.
Johnson Raymond (Mount Longstanton MD GB2) Livingston Dwight (Baltimore MD) Tite Robert C. (Baltimore MD) Wood-Helie Sheila J. (Hampstead MD), Disposable device for use in chemical, immunochemical and microorganism analysis.
Burns Rickey D. (Alpharetta GA) Heitz Bernhard H. (Woodstock GA) Meincke Drew F. (Lawrenceville GA) Wentzel Karl H. (Roswell GA), Disposable liquid reagent cartridge and receptacle therefor.
Bryan Kluttz ; Lawrence Burg ; Garry Tegeler ; Louis Graziano ; Christopher Cotter ; Michel Guy ; James Greer ; Geoff McKinley ; Luigi Catanzariti ; Robert Glassfold ; James Clement Bishop , Disposable test devices for performing nucleic acid amplification reactions.
Sutton Richard C. (Rochester NY) Ponticello Ignazio S. (Pittsford NY) Cummins Thomas J. (Rochester NY) Zander Dennis R. (Penfield NY) Donish William H. (Rochester NY), Element and method for nucleic acid amplification and detection using adhered probes.
Donald J. Hayes ; David B. Wallace ; Christopher J. Frederickson, Flexible apparatus with ablation formed chamber(s) for conducting bio-chemical analyses.
Walker G. Terrance (Chapel Hill NC) Nadeau James G. (Chapel Hill NC) Linn C. Preston (Durham NC), Fluorerscence polarization detection of nucleic acid amplication.
Belt William E. (Kansas City MO) Avery C. Frederick (Rockford IL) Klein Vernon W. (Kansas City MO), Gas generator/indicator unit adapted for use in an upright position.
White Raymond L. (Salt Lake City UT) Nakamura Yusuke (Salt Lake City UT) O\Connell Peter (Midvale UT) Leppert Mark F. (Salt Lake City UT), Genetic identification employing DNA probes of variable number tandem repeat loci.
Potter Derek Henry,GBX ; Potter Colin Gerald,GBX ; Old John Michael,GBX ; Bell John Irving,GBX, Method and apparatus for temperature control of multiple samples.
Dunn James M.,CAX ; Leushner James,CAX ; Renfrew John,CAX ; Waterhouse Paul,CAX ; Izmailov Alexandre M.,CAX ; Zaleski Henryk,CAX, Method and apparatus for thermal cycling and for automated sample preparation with thermal cycling.
Katamine Tomoaki (Urawa JPX) Sato Hiroshi (Kitakatsushika JPX) Mochida Ei (Tokyo JPX), Method and device for measuring a target substance in a liquid sample.
Fouillet, Yves; Vauchier, Claude; Clerc, Jean-Frederic; Peponnet, Christine; Claustre, Patricia; Charles, Raymond; Sarrut, Nicolas, Method for carrying out a biochemical protocol in continuous flow in a microreactor.
Woudenberg Timothy W. ; Albin Michael ; Kowallis Reid B. ; Raysberg Yefim ; Ragusa Robert P. ; Winn-Deen Emily S., Method for multiple analyte detection.
Sutton Richard Calvin ; Ponticello Ignazio Salvatore ; Cummins Thomas Joseph ; Zander Dennis Roland ; Donish William Harold ; Chen Paul Hong-Dze ; Findlay John Bruce, Method for nucleic acid amplification and detection using adhered probes.
Adams Christopher P. (Winter Hill MA) Kron Stephen Joseph (Boston MA), Method for performing amplification of nucleic acid with two primers bound to a single solid support.
Chen Yan (Palo Alto CA) Rose Samuel J. (Los Altos CA) Ullman Edwin F. (Atherton CA), Method for preventing amplification of nucleic acid contaminants in amplification mixtures using nuclease-receptor conju.
McMillan, William A.; Christel, Lee A.; Borkholder, David A.; Young, Steven J., Method for quantitative analysis of a nucleic acid amplification reaction.
Kikuchi Masayoshi (Tsukuba JPX) Kunai Kenji (Ushiku JPX) Yamada Takafumi (Sagamihara JPX), Method of assaying fibrinogen, dry reagent therefor, and process for the preparation thereof.
Kourilsky Philippe (Paris FRX) Avrameas Stratis (Paris FRX) Cami nee Contamine Brigitte (Paris FRX) Guesdon Jean-Luc (Paris FRX), Method of detecting and characterizing a nucleic acid or reactant for the application of this method.
Becker Michael (Palatine IL) Chase Jim (Grayslake IL) Papa Christopher (Naperville IL) Jones Jeff (Antioch IL), Method of making and filling a multi-chamber container.
Ryder Thomas Brendan ; Shannon Karen W. ; Kacian Daniel Louis ; Harvey Richard C. ; McDonough Sherrol H. ; Gonzales Frank R. ; Castillo Maria R. ; Billyard Elizabeth R. ; Shen Nancy Lau Liu, Methods for determining pre-amplification levels of a nucleic acid target sequence from post-amplification levels of pr.
McDonough Sherrol H. ; Kacian Daniel L. ; Dattagupta Nanibhushan ; McAllister Diane L. ; Hammond Philip W. ; Ryder Thomas B., Methods of amplifying nucleic acids using promoter-containing primer sequence.
Bernhard H. Weigl ; Paul Yager ; James P. Brody ; Mark R. Holl ; Fred K. Forster ; Eric Altendorf ; Paul C. Galambos ; Margaret Kenny ; David Schutte ; Gregory Hixson ; Diane Zebert ; Andr, Microfabricated devices and methods.
Weigl Bernhard H. ; Yager Paul ; Brody James P. ; Holl Mark R. ; Kenny Margaret ; Schutte David ; Hixson Gregory ; Zebert M. Diane ; Kamholz Andrew ; Wu Caicai ; Altendorf Eric, Microfabricated diffusion-based chemical sensor.
Robert J. Nelson ; Herbert H. Hooper ; Alan K. Hauser ; Sharat Singh ; Stephen J. Williams ; Alexander P. Sassi, Microfluidic method for nucleic acid purification and processing.
Haff Lawrence A. ; Picozza Enrico ; Bloch Will ; Ragusa Robert ; DiCesare Joseph ; Tracy David ; Saviano Paul ; Woudenberg Timothy M., Nucleic acid amplification reaction apparatus.
Haff Lawrence A. ; Picozza Enrico ; Bloch Will ; Ragusa Robert ; DiCesare Joseph ; Tracy David ; Saviano Paul ; Woudenberg Timothy M. ; Noreiks Richard W., Nucleic acid amplification reaction apparatus and method.
Bryan W. Kluttz ; Geoff A. McKinley ; Fabio Gennari IT; Michel Guy ; Christopher Cotter ; Luigi Catanzariti ; Louis Graziano ; Bruno Colin FR; Cecile Jaravel FR; Jacques Dachaud FR, Nucleic acid amplification reaction station for disposable test devices.
Chen Paul H.-D. (Boston MA) Findlay John B. (Rochester NY) Atwood Susan M. (Newark NY) Bergmeyer Lynn (Rochester NY), Nucleic acid material amplification and detection without washing.
Urdea Michael S. (Alamo CA) Warner Brian (Martinez CA) Horn Thomas (Berkeley CA), Nucleic acid multimers and amplified nucleic acid hybridization assays using same.
Hogan James (San Diego CA) Smith Richard (San Diego CA) Kop Joann (San Marcos CA), Nucleic acid probes for detection and/or quantitation of non-viral organisms.
Vogel Peter (Hemsbach DEX) Braun Hans-Peter (Hemsbach DEX) Berger Dieter (Viernheim DEX) Werner Wolfgang (Mannheim DEX), Process and composition for separating plasma or serum from whole blood.
Mullis Kary B. (Kensington CA) Erlich Henry A. (Oakland CA) Arnheim Norman (Woodland Hills CA) Horn Glenn T. (Emeryville CA) Saiki Randall K. (Richmond CA) Scharf Stephen J. (Berkeley CA), Process for amplifying, detecting, and/or-cloning nucleic acid sequences.
DeVaney ; Jr. Mark J. (Rochester NY) Lercher John S. (Rochester NY) Wellman Jeffrey A. (Rochester NY), Processing apparatus for a chemical reaction pack.
Wang Alice M. (Walnut Creek CA) Doyle Michael V. (Oakland CA) Mark David F. (Plainsboro NJ), Quantitation of nucleic acids using the polymerase chain reaction.
Grandone Cass J. (Lake Forest IL) Zuck Gary L. (Prospect Heights IL) Herchenbach Stephen L. (Grayslake IL) Tyranski James T. (Oakwood Hills IL) Nelson Robert J. (Highland Park IL), Reagent pack for immunoassays.
Weigl Bernhard H. ; Holl Mark R. ; Zebert Diane ; Kenny Margaret ; Wu Caicai, Simultaneous analyte determination and reference balancing in reference T-sensor devices.
Weigl Bernhard H. ; Holl Mark R. ; Zebert Diane ; Kenny Margaret ; Wu Caicai, Simultaneous analyte determination and reference balancing in reference T-sensor devices.
Shen Nancy Lau Liu ; Kacian Daniel Louis ; Putnam James Garfield ; Davis William Michael, Stabilized enzyme compositions for nucleic acid amplification.
Mullis Kary B. (LaJolla CA) Johnson Larry (San Jose CA) Leath Richard A. (Berkley CA) Wennberg Timothy J. (Mariposa CA) Mezei Louis M. (Madison WI) Widunas Joseph T. (Freemont CA), System for automated performance of the polymerase chain reaction.
Devaney ; Jr. Mark J. (Eastman Kodak Co. Rochester NY 14650-2201) Wellman Jeffrey A. (Eastman Kodak Co. Rochester NY 14650-2201) Lercher John S. (Eastman Kodak Co. Rochester NY 14650-2201), Temperature control device and reaction vessel.
Columbus Richard L. (Rochester NY) Helfer Jeffrey L. (Webster NY) Porte Johannes J. (Webster NY) Wellman Jeffrey A. (Rochester NY), Temperature cycling cuvette.
Atwood,John Girdner; Mossa,Albert Carmelo; Goven,Lisa May; Williams,Fenton; Woudenberg,Timothy M.; Margulies,Marcel; Ragusa,Robert P.; Leath,Richard; Miles,Clive, Thermal cycler for automatic performance of the polymerase chain reaction with close temperature control.
Weisburg, William G.; Shaw, Jay H.; Becker, Michael M.; Majlessi, Mehrdad R.; Brentano, Steven T.; Nunomura, Kiyotada, Two-step hybridization and capture of a polynucleotide.
Ismagilov, Rustem F.; Shen, Feng; Kreutz, Jason E.; Sun, Bing; Du, Wenbin, Analysis devices, kits, and related methods for digital quantification of nucleic acids and other analytes.
Ludwig, Lester, Chemical synthesis and analysis via integrated, sequential and series-parallel photochemical and other chemical processes for microfluidic, lab-on-a-chip, and green-chemistry applications.
Ismagilov, Rustem F.; Shen, Feng; Kreutz, Jason E.; Du, Wenbin; Sun, Bing, Multivolume devices, kits and related methods for quantification and detection of nucleic acids and other analytes.
Ismagilov, Rustem F.; Shen, Feng; Kreutz, Jason E.; Du, Wenbin; Sun, Bing, Multivolume devices, kits and related methods for quantification and detection of nucleic acids and other analytes.
Ismagilov, Rustem F.; Shen, Feng; Kreutz, Jason E.; Du, Wenbin; Sun, Bing, Multivolume devices, kits and related methods for quantification of nucleic acids and other analytes.
Opalsky, David; Walker, George T.; Nelson, Norman C.; Lee, Richard S.; Fan, Sara H., Systems and methods for detecting a signal and applying thermal energy to a signal transmission element.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.