Methods of potentiating HIV-1-specific CD8+ immune responses involving the concomitant administration of DNA and ALVAC expression vectors
원문보기
IPC분류정보
국가/구분
United States(US) Patent
등록
국제특허분류(IPC7판)
A61K-039/12
A61K-039/21
A61K-039/275
C07H-021/04
C12N-007/04
출원번호
UP-0428815
(2006-07-05)
등록번호
US-7771729
(2010-08-30)
발명자
/ 주소
Franchini, Genoveffa
Hel, Zdenek
Pavlakis, George
Tartaglia, James
출원인 / 주소
The United States of America as represented by the Department of Health and Human Services
대리인 / 주소
Sheridan Ross PC
인용정보
피인용 횟수 :
0인용 특허 :
31
초록▼
This invention relates to improved methods of inducing an immune response for the prevention or treatment of HIV-1 infection by using a nucleic acid vaccine in conjunction with a recombinant viral vaccine, e.g., a poxvirus vaccine, to potentiate and broaden the immune response. The present invention
This invention relates to improved methods of inducing an immune response for the prevention or treatment of HIV-1 infection by using a nucleic acid vaccine in conjunction with a recombinant viral vaccine, e.g., a poxvirus vaccine, to potentiate and broaden the immune response. The present invention further provides a particularly effective vaccine regimen comprising a DNA vaccine used in combination with a poxvirus virus, especially NYVAC or ALVAC.
대표청구항▼
What is claimed is: 1. A method of potentiating a CD8+ response to human immunodeficiency virus-1 (HIV-1) epitopes in a human comprising: administering an expression vector encoding HIV-1 Gag, Pol, Pro, Tat, Nef, Rev, Vif, Vpr or Env antigens; and administering a recombinant ALVAC pox virus encodin
What is claimed is: 1. A method of potentiating a CD8+ response to human immunodeficiency virus-1 (HIV-1) epitopes in a human comprising: administering an expression vector encoding HIV-1 Gag, Pol, Pro, Tat, Nef, Rev, Vif, Vpr or Env antigens; and administering a recombinant ALVAC pox virus encoding the same antigens encoded by the expression vector; wherein the expression vector and the recombinant pox virus enter the cells of the human and intracellularly produce HIV peptides that are presented on the cell's MHC class I molecules in an amount sufficient to stimulate a CD8+ response, and further, wherein administration of the combination of the expression vector and the recombinant pox virus potentiates the immune response compared to administration of either the expression vector or the recombinant pox virus by itself. 2. The method of claim 1, wherein the recombinant pox virus is an attenuated recombinant pox virus vaccine. 3. The method of claim 1, wherein the expression vector is a DNA expression vector. 4. The method of claim 1, wherein the HIV peptides are structural viral peptides. 5. The method of claim 1, wherein the HIV peptides are non-structural viral peptides. 6. The method of claim 1, wherein the expression vector or the recombinant ALVAC pox virus is administered with an adjuvant. 7. The method of claim 1, further comprising two administrations of the expression vector. 8. The method of claim 1, further comprising three administrations of the expression vector. 9. The method of claim 1, wherein the expression vector is administered before the recombinant pox virus. 10. The method of claim 1, wherein the human is infected with HIV-1. 11. The method of claim 10, wherein the human has a viral load of less than 10,000 copies per milliliter. 12. The method of claim 1, wherein the human is not infected with the HIV-1. 13. The method of claim 1, wherein the HIV peptides are HIV-1 envelope, gag or protease peptides. 14. A method of reducing viral load in a mammal that is infected with an immunodeficiency virus, comprising: administering to the mammal an expression vector that expresses encoded immunodeficiency virus Gag, Pol and Env antigens; and later administering to the mammal a recombinant ALVAC pox virus encoding said antigens; wherein the expression vector and the recombinant pox virus enter the cells of the mammal and intracellularly produce said antigens, and wherein administration of the combination of the expression vector and the recombinant pox virus reduces the viral load more than administration of either the expression vector or the recombinant pox virus by itself.
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이 특허에 인용된 특허 (31)
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