The invention disclosed herein comprises methods of using microarrays to simplify analysis and characterization of genes and their function. In one aspect of the invention the methods are used to identify and characterize antibodies having binding affinity for a specific target antigen. The inventio
The invention disclosed herein comprises methods of using microarrays to simplify analysis and characterization of genes and their function. In one aspect of the invention the methods are used to identify and characterize antibodies having binding affinity for a specific target antigen. The invention further comprises a method of determining gene expression at the protein level comprising contacting an array of characterized or uncharacterized antibodies on a solid surface with one or more proteins and identifying the antibodies to which said protein(s) binds. This method can be additionally used to compare the protein expression in two different populations of cells, such as normal cells and cancer cells or resting cells and stimulated cells. A further aspect of the invention comprises a method of determining gene expression at the protein level comprising contacting a microarray of nucleic acid samples derived from a variety of different sources with one or more nucleic acid probes then identifying the sample or samples to which the probe binds.
대표청구항▼
That which is claimed is: 1. A kit comprising: at least one antibody array, said antibody array comprising a plurality of discrete locations arrayed in a spatially addressable ordered matrix on a solid surface, wherein each of the discrete locations comprises one or more spots of an antibody bound
That which is claimed is: 1. A kit comprising: at least one antibody array, said antibody array comprising a plurality of discrete locations arrayed in a spatially addressable ordered matrix on a solid surface, wherein each of the discrete locations comprises one or more spots of an antibody bound to the solid surface, wherein a plurality of the discrete locations contain antibodies that are different from the antibodies of the other discrete locations; a first detectable label for labeling a first protein sample; optionally a second detectable label for detectably labeling a second protein sample, wherein the second detectable label is different from the first detectable label; and instructions for using the kit to perform one or more of the following: i. identifying antigens in the first or the second protein samples that bind to one or more of the antibodies bound to the solid surface; ii. comparing the protein expression profiles of two or more protein samples; iii. characterizing the first or the second protein samples based on the protein expression profile thereof; and iv. determining the effect of varying binding conditions on the affinity of the bound antibodies to antigens present in first or second protein samples. 2. The kit according to claim 1, wherein the array comprises up to about 600 discrete locations per square centimeter. 3. The kit according to claim 1, wherein the array comprises up to about 1000 discrete locations. 4. The kit according to claim 1, wherein the array comprises at least 1000 discrete locations. 5. The kit according to claim 1, wherein each spot contains between about 1 ng to about 1 μg of antibody. 6. The kit according to claim 1, wherein the antibodies are covalently bound to the solid surface. 7. The kit according to claim 1, further comprising a second antibody array. 8. The kit according to claim 1, wherein each detectable label is a fluorescent dye, biotin, an epitope tag or a colorimetric enzyme. 9. The kit according to claim 1, wherein each detectable label is a fluorescent dye. 10. The kit according to claim 9, wherein each detectable label is selected from the list fluorescein isothiocyanate (FITC), fluorescein, rhodamine, tetramethyl-rhodamine-5-(and 6)-isothiocyanate (TRITC), Texas red, a cyanine dye, Cy3 and Cy5. 11. The kit according to claim 1, wherein each discrete location in the array comprises two or more discrete spots of an antibody bound to the solid surface. 12. The kit according to claim 11, wherein each of the two or more discrete spots contains a different amount of antibody bound to the solid surface. 13. The kit according to claim 11, wherein each of the two or more discrete spots contains the same amount of antibody bound to the solid surface. 14. The kit according to claim 1, wherein each discrete location in the array comprises three or more discrete spots of an antibody bound to the solid surface. 15. The kit according to claim 1, wherein the discrete locations on the solid surface contain different antibodies. 16. The kit according to claim 1, wherein at least a portion of the discrete locations on the solid surface contain the same antibodies. 17. The kit according to claim 1, wherein at least a portion of the antibodies bound to the solid surface are uncharacterized. 18. The kit according to claim 1, wherein at least a portion of the antibodies bound to the solid surface are characterized. 19. The kit according to claim 1, wherein the antibodies in each spot are independently polyclonal antibodies, monoclonal antibodies, antibody fragments, single claim antibodies, humanized antibodies or recombinant antibodies. 20. The kit according to claim 1, further comprising an aqueous solution for incubating a sample with the antibody array. 21. The kit according to claim 1, further comprising a blocking solution. 22. The kit according to claim 1, further comprising a washing buffer. 23. The kit according to claim 1, wherein the surface comprises one or more of a plastic, a complex carbohydrate, an acrylic resin, nitrocellulose, glass, silicon or nylon.
연구과제 타임라인
LOADING...
LOADING...
LOADING...
LOADING...
LOADING...
이 특허에 인용된 특허 (192)
Soane David S. ; Soane Zoya M., Acrylic microchannels and their use in electrophoretic applications.
Ribi Hans O. (Hillsborough CA) Guion Todd (San Mateo CA) Shafer Paul T. (Campbell CA), Analyte detection with multilayered bioelectronic conductivity sensors.
Kornguth Steven E. (1114 Shorewood Blvd. Madison WI 53705) Corn Robert M. (4018 Manitou Way Madison WI 53711) Jordan Claire E. (707 W. Main St. Madison WI 53715) Frey Brian L. (526 Spruce St. Madison, Analytical biosensor.
Zentgraf, Hanswalter; Tessmer, Claudia; Velhagen, Iris; Schwinn, Susanne; Frey, Manfred, Antibodies active against a fusion polypeptide comprising a histidine portion.
Herron James N. (Salt Lake City UT) Christensen Douglas A. (Salt Lake City UT) Wang Hsu-Kun (Salt Lake City UT) Caldwell Karin D. (Salt Lake City UT) Janatova Vera (Prague CSX) Huang Shao-Chie (Salt , Apparatus and methods for multi-analyte homogeneous fluoro-immunoassays.
Chee Mark ; Gingeras Thomas R. ; Fodor Stephen P. A. ; Hubble Earl A. ; Morris MacDonald S., Array of nucleic acid probes on biological chips for diagnosis of HIV and methods of using the same.
Fodor Stephen P. A. (Palo Alto CA) Pirrung Michael C. (Durham NC) Read J. Leighton (Palo Alto CA) Stryer Lubert (Stanford CA), Array of oligonucleotides on a solid substrate.
Chee Mark ; Cronin Maureen T. ; Fodor Stephen P. A. ; Huang Xiaohua X. ; Hubbell Earl A. ; Lipshutz Robert J. ; Lobban Peter E. ; Morris MacDonald S. ; Sheldon Edward L., Arrays of nucleic acid probes on biological chips.
Ekins Roger P. (Department of Molecular Endocrinology University College and Middlesex School of Medicine Mortimer Street London W1N 8AA GBX) Chu Frederick W. (Department of Molecular Endocrinology U, Binding assay employing labelled reagent.
Olinger Jill M. (Fishers IN) Sligar Stephen G. (Urbana IL) McLean Mark A. (Champaign IL) Bohn Paul W. (Champaign IL) Stayton Patrick (Seattle WA), Coated substrates employing oriented layers of mutant heme proteins and methods of making and using same.
Winkler James L. (Palo Alto CA) Fodor Stephen P. A. (Palo Alto CA) Buchko Christopher J. (Ann Arbor MI) Ross Debra A. (Fremont CA) Aldwin Lois (San Mateo CA) Modlin Douglas N. (Palo Alto CA), Combinatorial strategies for polymer synthesis.
Winkler James L. ; Fodor Stephen P. A. ; Buchko Christopher J. ; Ross Debra A. ; Aldwin Lois ; Modlin Douglas N., Combinatorial strategies for polymer synthesis.
Winkler, James L.; Fodor, Stephen P. A.; Buchko, Christopher J.; Ross, Debra A.; Aldwin, Lois; Modlin, Douglas N., Combinatorial strategies for polymer synthesis.
Parekh Rajesh Bhikhu,GBX ; Amess Robert,GBX ; Bruce James Alexander,GBX ; Prime Sally Barbara,GBX ; Platt Albert Edward,GBX ; Stoney Richard Michael,GBX, Computer-assisted methods and apparatus for identification and characterization of biomolecules in a biological sample.
Haugland Richard P. (Eugene OR) Yue Stephen T. (Eugene OR) Millard Paul J. (Eugene OR) Roth Bruce L. (Corvallis OR), Cyclic-substituted unsymmetrical cyanine dyes.
Sundberg Steven A. ; Jacobs Jeffrey W. ; Schullek John R. ; McGall Glenn H. ; Mirzabekov Andrei ; Timofeey Edward,RUX ; Fujimoto David ; Holmes Christopher P. ; Yang Hua, Derivatization of solid supports and methods for oligomer synthesis.
Hanagan Ted J. (Libertyville IL) Safford Lance K. (Park City IL) Schultz Steven G. (Winthrop Harbor IL) Ford Jay R. (Mt. Prospect IL) Marciniec Edmund T. (Libertyville IL) Johnson Kenneth S. (Buffalo, Diagnostic flow cell device.
Zuk Robert F. (San Francisco CA) Gibbons Ian (Menlo Park CA) Rowley Gerald L. (Cupertino CA) Ullman Edwin F. (Atherton CA), Double antibody for enhanced sensitivity in immunoassay.
Zanzucchi Peter John (West Windsor Township NJ) Cherukuri Satyam Choudary (Cranbury NJ) McBride Sterling Edward (Lawrence Township NJ), Etching to form cross-over, non-intersecting channel networks for use in partitioned microelectronic and fluidic device.
Swedberg Sally A. (Los Altos CA) Kaltenbach Patrick (Bischweier DEX) Witt Klaus E. (Keltern DEX) Bek Fritz (Waldbronn DEX) Mittelstadt Laurie S. (Belmont CA), Fully integrated miniaturized planar liquid sample handling and analysis device.
Ogata Naoya (Tokyo JPX), High modulus blend of poly(a
상세보기
Schnur Joel M. (6009 Lincolnwood Ct. Burke VA 22015) Schoen Paul E. (5006 Taney Ave. Alexandria VA 22304) Peckerar Martin C. (12917 Buccaneer Rd. Silver Spring MD 20904) Marrian Christie R. K. (6805 , High resolution patterning on solid substrates.
Kallury Krishna M. R. (Scarborough CAX) Thompson Michael (Mississauga CAX) Lee William E. (Medicine Hat CAX), Immobilization of biologically active protein on a support with a 7-18 carbon spacer and a bifunctional phospholipid.
Pirrung Michael C. (Durham NC) Read J. Leighton (Palo Alto CA) Fodor Stephen P. A. (Palo Alto CA) Stryer Lubert (Stanford CA), Large scale photolithographic solid phase synthesis of an array of polymers.
Pirrung Michael C. (Durham NC) Read J. Leighton (Palo Alto CA) Fodor Stephen P. A. (Palo Alto CA) Stryer Lubert (Stanford CA), Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof.
Matson Robert S. (Orange CA) Coassin Peter J. (San Juan Capistrano CA) Rampal Jang B. (Yorba Linda CA) Southern Edwin M. (Kidlington GB2), Method and apparatus for creating biopolymer arrays on a solid support surface.
Stavrianopoulos Jannis G. (New York NY) Kirtikar Dollie (Elmhurst NY) Johnston Kenneth H. (New York NY) Thalenfeld Barbara E. (New York NY), Method and structures employing chemically-labelled polynucleotide probes.
Cherukuri Satyam C. (Cranbury NJ) Demers Robert R. (Cranbury NJ) Fan Zhong H. (Middlesex NJ) Levine Aaron W. (Lawrenceville NJ) McBride Sterling E. (Lawrence Township ; Mercer County NJ) Zanzucchi Pe, Method and system for inhibiting cross-contamination in fluids of combinatorial chemistry device.
Clark Noel A. (Boulder CO) Douglas Kenneth (Boulder CO) Rothschild Kenneth J. (Newton MA), Method for parallel fabrication of nanometer scale multi-device structures.
Mirzabekov Andrei Darievich,RUX ; Lysov Yuri Petrovich,RUX ; Dubley Svetlana A.,RUX, Method for performing site-specific affinity fractionation for use in DNA sequencing.
Singhvi Rahul ; Kumar Amit ; Whitesides George M. ; Ingber Donald E. ; Lopez Gabriel P. ; Wang Daniel I. C. ; Stephanopoulos Gregory N., Method of formation of microstamped patterns on plates for adhesion of cells and other biological materials, devices an.
Ershov Gennady Moiseevich,RUX ; Timofeev Eduard Nikolaevich,RUX ; Ivanov Igor Borisovich,RUX ; Florentiev Vladimir Leonidovich,RUX ; Mirzabekov Andrei Darievich,RUX, Method of immobilizing water-soluble bioorganic compounds on a capillary-porous carrier.
Lee Stephen C. (St. Louis MO) Leusch Mark S. (Manchester MO) Luckow Verne A. (Chesterfield MO) Olins Peter O. (Glencoe MO), Method of producing recombinant eukaryotic viruses in bacteria.
Wilding Peter (Paoli PA) Kricka Larry J. (Berwyn PA) Zemel Jay N. (Jenkintown PA), Methods and apparatus for the detection of an analyte utilizing mesoscale flow systems.
Sosnowski Ronald G. ; Butler William F. ; Tu Eugene ; Nerenberg Michael I. ; Heller Michael J. ; Edman Carl F., Methods and procedures for molecular biological analysis and diagnostics.
Zebedee, Suzanne; Inchauspe, Genevieve; Nasoff, Marc S.; Prince, Alfred S.; Helting, Torsten B.; Nunn, Michael F., Methods and systems for producing recombinant viral antigens.
Fodor Stephen P. A. ; Stryer Lubert ; Winkler James L. ; Holmes Christopher P. ; Solas Dennis W., Methods for determining receptor-ligand binding using probe arrays.
Mirzabekov Andrei Darievich,RUX ; Proudnikov Dimitri Y.,RUX ; Timofeev Edward N.,RUX ; Kochetkova Svetlana V.,RUX ; Florentiev Vladimir L.,RUX ; Shick Valentine V.,RUX, Methods for immobilizing nucleic acids on a gel substrate.
Rava Richard P. (San Jose CA) Fodor Stephen P. A. (Palo Alto CA) Trulson Mark (San Jose CA), Methods for making a device for concurrently processing multiple biological chip assays.
Unger, Marc A.; Chou, Hou-Pu; Thorsen, Todd A.; Scherer, Axel; Quake, Stephen R.; Enzelberger, Markus M.; Adams, Mark L.; Hansen, Carl L., Microfabricated elastomeric valve and pump systems.
Ekstrm Bjrn (Upsala SEX) Jacobson Gunilla (Upsala SEX) Ohman Ove (Upsala SEX) Sjdin Hakan (Upsala SEX), Microfluidic structure and process for its manufacture.
Bamdad Cynthia C. (Sharon MA) Sigal George B. (Arlington MA) Strominger Jack L. (Lexington MA) Whitesides George M. (Newton MA), Molecular recognition at surfaces derivatized with self-assembled monolayers.
Feickert Hans-Joachim (New York NY) Rettig Wolfgang (New York NY) Chorney Karen (New York NY) Cordon-Cardo Carlos (New York NY) Melamed Myron R. (Scarsdale NY) Lloyd Kenneth O. (Bronx NY) Oettgen Her, Monoclonal antibodies to human lung cancers and method.
Wohlstadter Jacob ; Wilbur James ; Sigal George ; Martin Mark ; Guo Liang-Hong ; Fischer Alan ; Leland Jon, Multi-array, multi-specific electrochemiluminescence testing.
Wohlstadter Jacob ; Wilbur James ; Sigal George ; Martin Mark ; Guo Liang-Hong ; Fischer Alan ; Leland Jon, Multi-array, multi-specific electrochemiluminescence testing.
Wohlstadter Jacob N. ; Wilbur James ; Sigal George ; Martin Mark ; Guo Liang-Hong ; Fischer Alan ; LeLand Jon, Multi-array, multi-specific electrochemiluminescence testing.
Eggers Mitchell D. (The Woodlands TX) Hogan Michael E. (The Woodlands TX) Beattie Kenneth L. (The Woodlands TX) Shumaker John (Houston TX) Ehrlich Daniel J. (Lexington MA) Hollis Mark (Concord MA), Multi-site detection apparatus.
Monforte Joseph Albert (Berkeley CA) Becker Christopher Hank (Menlo Park CA) Shaler Thomas Andrew (San Francisco CA) Pollart Daniel Joseph (Menlo Park CA), Oligonucleotide sizing using immobilized cleavable primers.
Hollis Mark A. (Concord MA) Ehrlich Daniel J. (Lexington MA) Murphy R. Allen (Boxboro MA) Kosicki Bernard B. (Acton MA) Rathman Dennis D. (Ashland MA) Chen Chang-Lee (Sudbury MA) Mathews Richard H. (, Optical and electrical methods and apparatus for molecule detection.
Zanzucchi Peter J. (Lawrenceville NJ) Cherukuri Satyam C. (Cranbury NJ) McBride Sterling E. (Lawrenceville NJ), Partitioned microelectronic and fluidic device array for clinical diagnostics and chemical synthesis.
Zanzucchi Peter J. (West Windsor Township NJ) Cherukuri Satyam C. (Cranbury NJ) McBride Sterling E. (Lawrence Township NJ) Judd Amrit K. (Belmont CA), Partitioned microelectronic device array.
Fodor Stephen P. A. (Palo Alto CA) Stryer Lubert (Stanford CA) Winkler James L. (Palo Alto CA) Holmes Christopher P. (Sunnyvale CA) Solas Dennis W. (San Francisco CA), Photolabile nucleoside and peptide protecting groups.
Siiman Olavi ; Burshteyn Alexander ; Gupta Ravinder K., Polymeric particles having a biodegradable gelatin or aminodextran coating and processes for making same.
Sparks Andrew B. ; Hoffman Noah ; Kay Brian K. ; Fowlkes Dana M. ; McConnell Stephen J., Polypeptides having a functional domain of interest and methods of identifying and using same.
Griffiths Andrew David,GBX ; Hoogenboom Hendricus Renerus Jacobus Mattheus,GBX ; Marks James David ; McCafferty John,GBX ; Winter Gregory Paul,GBX ; Grigg Geoffrey Walter,AUX, Production of anti-self antibodies from antibody segment repertoires and displayed on phage.
Cabilly Shmuel (Monrovia CA) Heyneker Herbert L. (Burlingame CA) Holmes William E. (Pacifica CA) Riggs Arthur D. (La Verne CA) Wetzel Ronald B. (San Francisco CA), Recombinant immunoglobin preparations.
Grandi Guido (Segrate ITX) De Ferra Francesca (Lodi ITX) Tosi Claudio (Busto Arsizio ITX) Tortora Ornella (Milan ITX) Cuzzoni Anna (Pavia ITX), Recombinant vector and use thereof for exocellular preparation of antibodies in single molecule form from Bacillus su.
Chrisey Linda A. (Bowie MD) Dressick Walter J. (Ft. Washington MD) Calvert Jeffrey M. (Alexandria VA), Selective attachment of nucleic acid molecules to patterned self-assembled surfaces.
Clark Noel A. (Boulder CO) Douglas Kenneth (Boulder CO) Rothschild Kenneth J. (Newton MA), Self-assembled nanometer lithographic masks and templates and method for parallel fabrication of nanometer scale multi-d.
Bergstrm Jan (Blinge SEX) Lfas Stefan (Uppsala SEX) Johnsson Bo (Storvreta SEX), Sensing surfaces capable of selective biomolecular interactions, to be used in biosensor systems.
Colapicchioni Claudio (Rome ITX) Porcelli Filippo (Rome ITX) Nuzzolo Carlo A. (Rome ITX), Sensor with antigen chemically bonded to a semiconductor device.
Barrett Ronald W. (Sunnyvale CA) Pirrung Michael C. (Durham NC) Stryer Lubert (Stanford CA) Holmes Christopher P. (Sunnyvale CA) Sundberg Steven A. (San Francisco CA), Spatially-addressable immobilization of anti-ligands on surfaces.
McGall Glenn H. (Mountain View CA) Fodor Stephen P. A. (Palo Alto CA) Sheldon Edward L. (Menlo Park CA), Spatially-addressable immobilization of oligonucleotides and other biological polymers on surfaces.
Fodor Stephen P. A. (Palo Alto CA) Pirrung Michael C. (Durham NC) Read J. Leighton (Palo Alto CA) Stryer Lubert (Stanford CA), Synthesis and screening of immobilized oligonucleotide arrays.
Merkh Carl W. (Lindenhurst IL) Defreese James D. (Wildwood IL) Durley ; III ; deceased Benton A. (late of Antioch IL by Robert W. Durley ; executrix), Test array for performing assays.
Yates ; III John R. (Seattle WA) Eng James K. (Seatac WA), Use of mass spectrometry fragmentation patterns of peptides to identify amino acid sequences in databases.
Fodor Stephen P. A. (Palo Alto CA) Stryer Lubert (Stanford CA) Pirrung Michael C. (Durham NC) Read J. Leighton (Palo Alto CA), Very large scale immobilized polymer synthesis.
Winkler James L. (Palo Alto CA) Fodor Stephen P. A. (Palo Alto CA) Buchko Christopher J. (Palo Alto CA) Ross Debra A. (Fremont CA) Aldwin Lois (San Mateo CA), Very large scale immobilized polymer synthesis using mechanically directed flow paths.
Herron James N. (Salt Lake City UT) Christensen Douglas A. (Salt Lake City UT) Caldwell Karin D. (Salt Lake City UT) Janatov Vera (Prague UT CSX) Huang Shao-Chie (Salt Lake City UT) Wang Hsu-Kun (Sal, Waveguide immunosensor with coating chemistry providing enhanced sensitivity.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.