DNA encoding a plant quinolate phosphoribosyl transferase (QPRTase) enzyme, and constructs comprising such DNA are provided. Methods of altering quinolate phosphoribosyl transferase expression are provided.
대표청구항▼
That which is claimed: 1. A tobacco product comprising a transgenic plant cell from a transgenic tobacco plant of the genus Nicotiana having reduced quinolate phosphoribosyl transferase (QPRTase) production relative to a non-transformed control plant, said transgenic plant comprising transgenic pla
That which is claimed: 1. A tobacco product comprising a transgenic plant cell from a transgenic tobacco plant of the genus Nicotiana having reduced quinolate phosphoribosyl transferase (QPRTase) production relative to a non-transformed control plant, said transgenic plant comprising transgenic plant cells comprising a heterologous nucleic acid; comprising a nucleotide sequence selected from the group consisting of: (a) the nucleotide sequence of SEQ ID NO:1; (b) a nucleotide sequence that encodes a quinolate phosphoribosyl transferase enzyme having the amino acid sequence of SEQ ID NO:2; (c) a nucleotide sequence that is at least 95% identical to the nucleotide sequence of (a) or (b) above and that encodes a quinolate phosphoribosyl transferase enzyme; and (d) a nucleotide sequence that differs from the nucleotide sequence of (a) or (b) above due to the degeneracy of the genetic code and that encodes a quinolate phosphoribosyl transferase enzyme. 2. A tobacco product comprising a transgenic plant cell from a transgenic tobacco plant of the genus Nicotiana having a decreased level of nicotine in leaves of said plant relative to a non-transformed control plant, wherein said plant comprises transgenic plant cells comprising a heterologous nucleic acid, comprising a nucleotide sequence selected from the group consisting of: (a) the nucleotide sequence of SEQ ID NO:1; (b) a nucleotide sequence that encodes a quinolate phosphoribosyl transferase enzyme having the amino acid sequence of SEQ ID NO:2; (c) a nucleotide sequence that is at least 95% identical to the nucleotide sequence of (a) or (b) above and that encodes a quinolate phosphoribosyl transferase enzyme; and (d) a nucleotide sequence that differs from the nucleotide sequence of (a) or (b) above due to the degeneracy of the genetic code and that encodes a quinolate phosphoribosyl transferase enzyme. 3. The tobacco product of claim 1, wherein said heterologous nucleic acid is in antisense orientation. 4. The tobacco product of claim 2, wherein said heterologous nucleic acid is in antisense orientation. 5. The tobacco product of claim 1, wherein said heterologous nucleic acid is in sense orientation. 6. The tobacco product of claim 2, wherein said heterologous nucleic acid is in sense orientation. 7. A tobacco product comprising a transgenic plant cell from a transgenic plant of the genus Nicotiana having reduced quinolate phosphoribosyl transferase (QPRTase) production relative to a non-transformed control plant, wherein said transgenic plant is a progeny of the plant of claim 1. 8. A tobacco product comprising a transgenic plant cell from a transgenic plant of the genus Nicotiana having reduced nicotine in leaves of said plant, wherein said transgenic plant is a progeny of the plant of claim 2. 9. The tobacco product of claim 1, wherein the tobacco product is selected from the group consisting of a cigarette, cigarette tobacco, cigar tobacco, a cigar, pipe tobacco, chewing tobacco, leaf tobacco, shredded tobacco and cut tobacco. 10. The tobacco product of claim 2, wherein the tobacco product is selected from the group consisting of a cigarette, cigarette tobacco, cigar tobacco, a cigar, pipe tobacco, chewing tobacco, leaf tobacco, shredded tobacco and cut tobacco. 11. The tobacco product of claim 3, wherein the tobacco product is selected from the group consisting of a cigarette, cigarette tobacco, cigar tobacco, a cigar, pipe tobacco, chewing tobacco, leaf tobacco, shredded tobacco and cut tobacco. 12. The tobacco product of claim 4, wherein the tobacco product is selected from the group consisting of a cigarette, cigarette tobacco, cigar tobacco, a cigar, pipe tobacco, chewing tobacco, leaf tobacco, shredded tobacco and cut tobacco. 13. The tobacco product of claim 5, wherein the tobacco product is selected from the group consisting of a cigarette, cigarette tobacco, cigar tobacco, a cigar, pipe tobacco, chewing tobacco, leaf tobacco, shredded tobacco and cut tobacco. 14. The tobacco product of claim 6, wherein the tobacco product is selected from the group consisting of a cigarette, cigarette tobacco, cigar tobacco, a cigar, pipe tobacco, chewing tobacco, leaf tobacco, shredded tobacco and cut tobacco. 15. The tobacco product of claim 7, wherein the tobacco product is selected from the group consisting of a cigarette, cigarette tobacco, cigar tobacco, a cigar, pipe tobacco, chewing tobacco, leaf tobacco, shredded tobacco and cut tobacco. 16. The tobacco product of claim 8, wherein the tobacco product is selected from the group consisting of a cigarette, cigarette tobacco, cigar tobacco, a cigar, pipe tobacco, chewing tobacco, leaf tobacco, shredded tobacco and cut tobacco.
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