초록 ▼

The present invention relates to an improved system for efficiently and accurately performing immunoassays, such as ELISAs. The invention provides an immunoassay assembly which includes a flow-through unit and an aspiration pump. The immunoassay flow-through unit includes an outer seal; at least one

The present invention relates to an improved system for efficiently and accurately performing immunoassays, such as ELISAs. The invention provides an immunoassay assembly which includes a flow-through unit and an aspiration pump. The immunoassay flow-through unit includes an outer seal; at least one bed support; an inner seal; and a packed non-porous bed. The unit is releasably attached to an aspiration pump which enables the controlled flow rate of liquid passing through the packed bed of the flow-through unit. The invention also provides a method of using the immunoassay assembly to identify analytical targets of interest.

대표청구항 ▼

We claim: 1. Apparatus for conducting an immunoassay or selective adsorption separation, the apparatus comprising: an assay unit having a passage formed in its center to form a packed bed; porous bed supports mounted in the assay unit at opposite ends of the packed bed; solid phase support beads lo

We claim: 1. Apparatus for conducting an immunoassay or selective adsorption separation, the apparatus comprising: an assay unit having a passage formed in its center to form a packed bed; porous bed supports mounted in the assay unit at opposite ends of the packed bed; solid phase support beads located in the packed bed and having a reaction reagent mounted on their surface; an outlet from the assay unit having an outer diameter and located below the packed bed; two frusto-conical sealing surfaces on an upper surface of the assay unit above and opening into the packed bed; and a frusto-conical sample cup positioned between the two frusto-conical sealing surfaces, wherein the sample cup has a volume of from about 5 to about 100 μL; wherein the two frusto-conical sealing surfaces comprise an upper sealing surface dimensioned to form a fluid-tight, friction seal with a pipette holder, and a lower sealing surface abutting the packed bed and dimensioned to form a fluid-tight, friction seal with a device selected from the group consisting of straight wall tubing have an outer diameter of from about 0.75 to about 1 mm, hypodermic needles having a gauge of from about 19 to 21, and pipette tips having a volume of from about 10 μL to about 200 μl; and wherein the outer diameter of the outlet from the assay unit is dimensioned to form a fluid-tight, friction seal with the lower sealing surface when two assay units are nested together. 2. The apparatus of claim 1, wherein the lower sealing surface is dimensioned to form a fluid-tight, friction seal with a pipette tip.