A microfluidic fluid flow system (100) is disclosed having a fluid chamber or channel (150) with inlet and outlet ports (104, 106), allowing the fluid channel to be filled with a fluid. One or more flow obstructions or perturbances, such as cylinders (152), are provided in the channel. An oscillator
A microfluidic fluid flow system (100) is disclosed having a fluid chamber or channel (150) with inlet and outlet ports (104, 106), allowing the fluid channel to be filled with a fluid. One or more flow obstructions or perturbances, such as cylinders (152), are provided in the channel. An oscillatory boundary condition is applied, for example, with a piezoelectric driver (130), that is selected to induce a conservative, low-intensity steady streaming flow in the channel. The low-intensity streaming flow produces distinct eddies that can be utilized, for example, for fluid-dynamically trapping or retaining particles (90) such as cells (92) at well defined locations in the channel. The system may be used to trap and study individual cells or for concentrating or filtering particles in a fluid.
대표청구항▼
The embodiments of the invention in which an exclusive property or privilege is claimed are defined as follows: 1. A method for fluid-dynamically trapping one or more particles in a fluid, the method comprising the steps of: providing a channel with at least one channel feature that causes deviatio
The embodiments of the invention in which an exclusive property or privilege is claimed are defined as follows: 1. A method for fluid-dynamically trapping one or more particles in a fluid, the method comprising the steps of: providing a channel with at least one channel feature that causes deviation from unidirectional flow; filling the channel with a fluid containing a plurality of particles having a diameter of at least 20 μm; and oscillating a boundary of the fluid at a frequency and an amplitude to generate a low-intensity steady streaming flow such that eddies are generated at fixed locations in the fluid, wherein the low-intensity steady streaming is driven by time-averaged Reynolds stresses resulting from inertial terms in the boundary layer in oscillating flow; wherein the fluid exerts a force that moves at least one of the plurality of particles into one of the eddies. 2. The method of claim 1, wherein the eddies are created by a conservative effect that does not require compression of the fluid. 3. The method of claim 1, wherein at least one of the eddies fluid dynamically retains at least one of the plurality of particles. 4. The method of claim 1, wherein some of the plurality of particles become concentrated within the eddies relative to the fluid in the channel away from the eddies. 5. The method of claim 1, wherein the channel feature is an obstruction in the channel. 6. The method of claim 5, wherein the channel includes a pair of oppositely disposed sidewalls, and the obstruction is disposed between the sidewalls and spaced apart from the sidewalls. 7. The method of claim 6, wherein the obstruction is a cylinder. 8. The method of claim 5, wherein the channel includes a pair of oppositely disposed sidewalls and the obstruction is a protrusion from one of the sidewalls. 9. The method of claim 1, wherein the channel further comprises at least one inlet port and at least one outlet port, and wherein there is a net fluid flow through the channel. 10. The method of claim 1, wherein the plurality of particles contained in the fluid are cells. 11. The method of claim 10, wherein the cells are motile and capable of exerting a motile force, and wherein the eddies generate a trapping force that is greater than the motile force of the cells. 12. The method of claim 1, wherein at least some of the plurality of particles contained in the fluid are denser than the fluid. 13. The method of claim 1, wherein the eddies generate a trapping force that is controlled by the oscillation amplitude. 14. The method of claim 1, wherein the size of the eddies is controlled by the oscillation frequency. 15. The method of claim 1, wherein the channel includes a plurality of channel features, with at least one eddy associated with each channel feature. 16. The method of claim 15, wherein at least one of the channel features is a cylindrical obstruction. 17. The method of claim 15, wherein the channel further comprises an inlet port and an outlet port, and wherein there is a net fluid flow through the channel. 18. The method of claim 1, further comprising the step of recovering the plurality of particles in the eddy. 19. A method of isolating cells at positions within a fluid, the method comprising: immersing at least one cell in a fluid; providing a channel having at least one channel feature that causes deviation from unidirectional flow; filling the channel with the fluid; and oscillating a boundary of the fluid at a frequency and an amplitude to generate a low-intensity steady streaming flow in the fluid such that an eddy is generated in the fluid, and fluid dynamically trapping at least one cell in the eddy, wherein the low-intensity steady streaming is driven by time-averaged Reynolds stresses resulting from inertial terms in the boundary layer in oscillating flow. 20. The method of claim 19, wherein the at least one channel feature is a cylinder. 21. The method of claim 19, wherein the at least one channel feature is a protrusion from a channel wall. 22. The method of claim 19, wherein the at least one cell is denser than the fluid. 23. The method of claim 19, wherein the at least one cell is motile and capable of generating a motile force, and wherein the eddy produces a trapping force that is greater than the motile force of the cell. 24. The method of claim 19, wherein the channel further comprises at least one inlet port and at least one outlet port, and wherein there is a net fluid flow through the channel. 25. The method of claim 19, wherein the eddy produces a trapping force that is controlled by the oscillation amplitude. 26. The method of claim 19, wherein the size of the eddy is controlled by the oscillation frequency. 27. The method of claim 19, wherein the at least one channel feature comprises a plurality of channel features, and wherein the low-intensity steady streaming generates at least one eddy associated with each channel feature. 28. The method of claim 27, wherein plurality of channel features are cylinders. 29. The method of claim 27, wherein the channel further comprises at least one inlet port and at least one outlet port, and wherein there is a net fluid flow through the channel. 30. The method of claim 19, further comprising the step of recovering the cell trapped in the eddy.
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이 특허에 인용된 특허 (3)
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