초록 ▼

Transgenic plants, plant tissue, and propagation materials are disclosed that exhibit or convey increased resistance to pathogens of multiple higher-order phylogenetic lineages. The disclosed transgenic plants and plant tissues include plant cells containing a DNA construct encoding Gastrodia Anti-F

Transgenic plants, plant tissue, and propagation materials are disclosed that exhibit or convey increased resistance to pathogens of multiple higher-order phylogenetic lineages. The disclosed transgenic plants and plant tissues include plant cells containing a DNA construct encoding Gastrodia Anti-Fungal Protein (GAFP), also known as gastrodianin, an anti-fungal gene naturally occurring in a Chinese orchid, Gastrodia elata. Transgenic plants disclosed include herbaceous plants as well as woody plants, including fruit trees. Disclosed transgenic plants can also be beneficially utilized as rootstock, for instance rootstock for stone fruit crops such as peach, thereby conferring enhanced disease resistance to the rootstock without genetically altering the scion.

대표청구항 ▼

What is claimed is: 1. A method for increasing the resistance of a plant to root pathogenic oomycetes comprising cultivating a transgenic plant selected from the group consisting of fruit trees, ornamental plants, and vegetables, the transgenic plant containing transformed plant cells, the transfor

What is claimed is: 1. A method for increasing the resistance of a plant to root pathogenic oomycetes comprising cultivating a transgenic plant selected from the group consisting of fruit trees, ornamental plants, and vegetables, the transgenic plant containing transformed plant cells, the transformed plant cells including a DNA construct encoding a gastrodianin protein in a sense orientation, the gastrodianin protein selected from the group consisting of GAFP-VNF protein, GAFP-VGM protein, GAFP-MGM protein, and GAFP-MNF protein, wherein the transgenic plant has been transformed from the wild-type plant so as to express the gastrodianin protein, and wherein the transgenic plant exhibits increased resistance to pathogenic oomycetes as compared to the same type of plant that does not express the gastrodianin protein. 2. The method of claim 1, wherein the transgenic plant exhibits increased resistance to multiple pathogens that cause disease in plant roots. 3. The method of claim 1, wherein the transgenic plant further exhibits increased resistance to fungi of the phylum Basidiomycota or Ascomycota. 4. The method of claim 1, wherein the transgenic plant further exhibits increased resistance to metazoa. 5. The method of claim 1, further comprising grafting a scion to the transgenic plant, wherein the scion does not express the gastrodianin protein. 6. The method of claim 5, further comprising harvesting a crop from the scion. 7. The method of claim 6, wherein the crop is an edible crop. 8. The method of claim 1, wherein the transgenic plant has been further transformed with a heterologous second DNA encoding a second protein. 9. The method of claim 8, wherein the second protein is either a gastrodianin or a protein that is different from the gastrodianin. 10. A transgenic rootstock containing transformed plant cells, the transformed plant cells including a DNA construct encoding a gastrodianin protein selected from the group consisting of GAFP-VNF protein, GAFP-VGM protein, GAFP-MGM protein, and GAFP-MNF protein, wherein the transgenic rootstock expresses the gastrodianin protein, and wherein the transgenic rootstock exhibits increased resistance to multiple pathogens as compared to the same type of rootstock that does not express the gastrodianin protein. 11. The transgenic rootstock of claim 1, wherein the gastrodianin protein is GAFP-VNF or GAFP-VGM. 12. The transgenic rootstock of claim 11, wherein the gastrodianin protein is GAFP-VNF. 13. The transgenic rootstock of claim 10, wherein the DNA construct includes a c-DNA that encodes the gastrodianin protein. 14. The transgenic rootstock of claim 10, wherein the transgenic rootstock expresses a second protein, wherein the wild-type rootstock does not express the second protein. 15. A transgenic plant containing transformed plant cells, the transformed plant cells including a DNA construct encoding a gastrodianin protein in a sense orientation, wherein the transgenic plant expresses the gastrodianin protein selected from the group consisting of gastrodianin-VNF protein, gastrodianin-VGM protein, gastrodianin-MGM protein, and gastrodianin-MNF protein, wherein the transgenic plant exhibits increased resistance to root pathogenic oomycetes as compared to the same type of plant that does not express the gastrodianin protein, and wherein the transgenic plant is selected from the group consisting of fruit trees, ornamental plants and vegetables. 16. The transgenic plant of claim 15, wherein the gastrodianin protein is GAFP-VNF or GAFP-VGM. 17. The transgenic plant of claim 16, wherein the gastrodianin protein is GAFP-VNF. 18. The transgenic plant of claim 15, wherein the DNA construct includes a c-DNA that encodes the gastrodianin protein. 19. The method according to claim 2, wherein the transgenic plant further exhibits increased resistance to a nematode.